BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0061.0001.p (436 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3598 [O] COG0265 Trypsin-like serine proteases, typically peri... 122 1e-27 lin2645 [O] COG0265 Trypsin-like serine proteases, typically per... 102 1e-21 BS_yvjD [O] COG0265 Trypsin-like serine proteases, typically per... 94 5e-19 CAC0500 [O] COG0265 Trypsin-like serine proteases, typically per... 54 5e-07 >BH3598 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 398 Score = 122 bits (305), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 71/226 (31%), Positives = 116/226 (51%), Gaps = 4/226 (1%) Query: 163 QGARLAAPLFLKGKRGKVVGGYQLEAFWPLPLFLLIPSGAGIGDLPWHPXXXXXXXXVS- 221 +G ++P+ ++ KRGK VG ++++ W LP+ L IP GA I + P + Sbjct: 165 RGGSFSSPVVVRSKRGKWVGFHRVKRLWLLPVVLFIPEGA-IPTFSFWPILTVAGVSLQP 223 Query: 222 --LPVIIGFSEMTQGLLPGRKSARTAGRXXXXXXXXXXXXXXXXXXXXXTVXXXXXXXXX 279 +P +IG LP S + + +V Sbjct: 224 LVVPFLIGTDFKIWSSLPKVVSRKVGNQVLGLALTLALIVCITWYVPWLSVLFAIVAIVG 283 Query: 280 XXXXGWYSALEERSLSPIFVHPPAGRKVLAVLPDSPAQELGILPGEILLKVNGVLLTDAA 339 + +++RS + +F + G KVL +LP SPA+++GI GE +L+VNG ++ + Sbjct: 284 REGIHLMAKIKDRSSTSLFTNQGTGCKVLGILPGSPAEKMGIKIGETILRVNGKMVNNEY 343 Query: 340 QLHEALRMNPAFCKLEVQNRAGESKYLQRAIYDGDHHQLGIILVPE 385 +EAL+ N AFC+LEV ++AGE ++ Q A+YDG+HHQLG++LV E Sbjct: 344 SFYEALQENSAFCRLEVIDKAGEVRFAQGALYDGEHHQLGVLLVKE 389 >lin2645 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 391 Score = 102 bits (254), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 83/320 (25%), Positives = 133/320 (41%), Gaps = 12/320 (3%) Query: 78 SLTGTAVAIIW-----AVSLVLMLF-RVRYLCFAYSIGLLGILQFILSFFPDTLQ--SGL 129 +L G +V I W AV+ L++ R A++IG+ ++ + +F L + L Sbjct: 72 TLIGFSVMIEWIAIFNAVACFLLIVGMFRLSSTAFTIGITSLIFYFCYYFDVNLSPFTNL 131 Query: 130 AGTIATALREMDIPXXXXXXXXXXXXXXXXXRWQGARLAAPLFLKGKRGKVVGGYQLEAF 189 T + + ++ A+ +P K KRGK+VG +QL Sbjct: 132 EFTYDAIFIDNFMISMTFLLAILLFVEAFLIQYTSAKNPSPSLRKSKRGKLVGSFQLRKL 191 Query: 190 WPLPLFLLIPSGAGIGDLPWHPXXXXXXXXVSL---PVIIGFSEMTQGLLPGRKSARTAG 246 W +PL + IP W P SL P+ IGF + Q LP S + A Sbjct: 192 WFVPLVVFIPGDVFTKIFEWWPVFTIGSESYSLIILPLFIGFQQQVQAQLPEEASKKIAV 251 Query: 247 RXXXXXXXXXXXXXXXXXXXXXTVXXXXXXXXXXXXXGWYSALEERSLSPIFVHPPAGRK 306 + T+ + E+ L F P G Sbjct: 252 QVTTLATIIAVAGLIGIVMPVLTLFAFMFAVIGRFWISYRHYRSEQLLPKKFGPQPDGLV 311 Query: 307 VLAVLPDSPAQELGILPGEILLKVNGVLLTDAAQLHEALRMNPAFCKLEVQNRAGESKYL 366 VL +P+ L + GE + +VNG + + +L+EAL +N AFCKL+V + AGE ++ Sbjct: 312 VLGARESTPSTRLNLKAGEKITEVNGQKVHNREELYEALNLNRAFCKLKVIDNAGEPRFE 371 Query: 367 QRAIYDGDHHQLGIILVPEP 386 Q A+Y+ + +LG++LV EP Sbjct: 372 QTALYENESFELGLLLV-EP 390 >BS_yvjD [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 397 Score = 93.6 bits (231), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 61/220 (27%), Positives = 95/220 (43%), Gaps = 3/220 (1%) Query: 169 APLFLKGKRGKVVGGYQLEAFWPLPLFLLIPSGAGIGDLPWHPXXXX---XXXXVSLPVI 225 +P + RG +G W LPLFLL+P L W P + +P Sbjct: 167 SPALVVSSRGLPIGQQLANRVWLLPLFLLVPGNGLESHLSWWPVFTVPGGSFHFLWIPYF 226 Query: 226 IGFSEMTQGLLPGRKSARTAGRXXXXXXXXXXXXXXXXXXXXXTVXXXXXXXXXXXXXGW 285 +GF + QG LP TA R Sbjct: 227 VGFGQRVQGSLPETSIRITAKRVCILGLAVAVLGAASLLWTPLAGAAVCTALLGRIFLSI 286 Query: 286 YSALEERSLSPIFVHPPAGRKVLAVLPDSPAQELGILPGEILLKVNGVLLTDAAQLHEAL 345 + + + F G VL ++P++PA++L + GEI+ KVNG+ + + + +EAL Sbjct: 287 KQRVNDNAAPFYFSKRDQGLMVLGIIPNTPAEDLELKIGEIITKVNGIPVKNVSDFYEAL 346 Query: 346 RMNPAFCKLEVQNRAGESKYLQRAIYDGDHHQLGIILVPE 385 + N A+ KLE+ GE ++ QRA Y+G+HH+LGI+ V + Sbjct: 347 QHNRAYVKLEIIGLNGEIRFDQRASYEGEHHELGILFVKD 386 >CAC0500 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 434 Score = 53.9 bits (128), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 29/117 (24%), Positives = 56/117 (47%), Gaps = 4/117 (3%) Query: 81 GTAVAIIWAVSLVLMLFRVRYLCFAYSIGLLGILQFILSFFPDTLQSGLAGTIATALREM 140 +A+ +++ +S++LM R+LCF+YS +LGI+ ++ F + +A + E+ Sbjct: 85 NSAIVLLFLISVILMFAGSRFLCFSYSGSILGIISIVIQFLS---VNKIADVSSIKFLEI 141 Query: 141 DIPXXXXXXXXXXXXXXXXXRWQGARLAAPLFLKGKRGKVVGGYQLEAFWPLPLFLL 197 D+ G A P+F + K++GG+ + +W LP+ LL Sbjct: 142 DVVMIMSLVGVLHIVEGIIVMIDGKSGAIPVF-TNREDKIIGGFAFKRYWVLPITLL 197 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,592,002 Number of extensions: 831103 Number of successful extensions: 2836 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2833 Number of HSP's successfully gapped: 4 Length of query: 436 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 327 Effective length of database: 25,807,025 Effective search space: 8438897175 Effective search space used: 8438897175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)