BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0013.p (312 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0088 [E] COG0031 Cysteine synthase 404 e-113 BS_cysK [E] COG0031 Cysteine synthase 396 e-110 SA0471 [E] COG0031 Cysteine synthase 387 e-107 BS_ytkP [E] COG0031 Cysteine synthase 349 3e-96 all2521 [E] COG0031 Cysteine synthase 329 3e-90 >BH0088 [E] COG0031 Cysteine synthase Length = 307 Score = 404 bits (1039), Expect = e-113, Method: Compositional matrix adjust. Identities = 200/309 (64%), Positives = 246/309 (79%), Gaps = 4/309 (1%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEGLL 62 +V NS+TDLIG TPLV+LNR+V + A++YLKLE+ NPGSSVKDRIA++++E AEK G L Sbjct: 2 RVANSITDLIGQTPLVKLNRLVSDKHADVYLKLEFFNPGSSVKDRIALAMIEAAEKSGAL 61 Query: 63 KPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGSEG 122 KP TIVE TSGNTGIGLA+VAAAKGYK +VMPETMSLERRNLLRAYGAELVLTPG EG Sbjct: 62 KPDDTIVEPTSGNTGIGLAMVAAAKGYKTKLVMPETMSLERRNLLRAYGAELVLTPGPEG 121 Query: 123 MNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXXXX 182 M GA++KA ++ EN YF+ +QF+N+AN +IHRETTG E++E ++ G LD F Sbjct: 122 MGGAIRKATELAQEN-GYFMPQQFENEANPEIHRETTGRELLEQVD---GQLDGFVSGIG 177 Query: 183 XXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNVYD 242 +LK +PN+ I AVEPA SP+L+GG PGPHKIQGIGA F+P IL+ ++YD Sbjct: 178 TGGTITGAGGLLKENFPNLHIAAVEPADSPVLSGGNPGPHKIQGIGAGFVPKILDTDIYD 237 Query: 243 EIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGERY 302 E+I V ++AFE ARR AKEEG+L GISSGAA++AAL+VA++LG GKKVV +IPSNGERY Sbjct: 238 EVIKVSTEQAFEYARRAAKEEGILGGISSGAAIYAALQVAEKLGPGKKVVAVIPSNGERY 297 Query: 303 LSTPLYNFE 311 LSTPLY FE Sbjct: 298 LSTPLYQFE 306 >BS_cysK [E] COG0031 Cysteine synthase Length = 308 Score = 396 bits (1018), Expect = e-110, Method: Compositional matrix adjust. Identities = 208/311 (66%), Positives = 251/311 (80%), Gaps = 3/311 (0%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 M +V NS+T+LIG TP+V+LNR+ E SA++YLKLEY NPGSSVKDRI ++++E AEKEG Sbjct: 1 MVRVANSITELIGNTPIVKLNRLADENSADVYLKLEYMNPGSSVKDRIGLAMIEAAEKEG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LK G TI+E TSGNTGIGLA+VAAAKG KA++VMP+TMS+ERRNLLRAYGAELVLTPG+ Sbjct: 61 KLKAGNTIIEPTSGNTGIGLAMVAAAKGLKAILVMPDTMSMERRNLLRAYGAELVLTPGA 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 EGM GA+KKAE+ L+E YF+ +QF N +N +IHR+TTG EIVE LDAF Sbjct: 121 EGMKGAIKKAEE-LAEKHGYFVPQQFNNPSNPEIHRQTTGKEIVEQFGD--DQLDAFVAG 177 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 EVLK YP++KIYAVEP+ SP+L+GGKPGPHKIQGIGA F+PDILN V Sbjct: 178 IGTGGTITGAGEVLKEAYPSIKIYAVEPSDSPVLSGGKPGPHKIQGIGAGFVPDILNTEV 237 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDEI V+N+EAFE ARR A+EEG+L GISSGAA++AAL+VAK+LG GKKV+ IIPSNGE Sbjct: 238 YDEIFPVKNEEAFEYARRAAREEGILGGISSGAAIYAALQVAKKLGKGKKVLAIIPSNGE 297 Query: 301 RYLSTPLYNFE 311 RYLSTPLY F+ Sbjct: 298 RYLSTPLYQFD 308 >SA0471 [E] COG0031 Cysteine synthase Length = 310 Score = 387 bits (993), Expect = e-107, Method: Compositional matrix adjust. Identities = 191/309 (61%), Positives = 243/309 (78%), Gaps = 3/309 (0%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEGLL 62 K V+++T +IGGTP+V+L VV + +A++Y+KLEYQNPG SVKDRIA++++E+AE+EG + Sbjct: 4 KPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGSVKDRIALAMIEKAEREGKI 63 Query: 63 KPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGSEG 122 KPG TIVE TSGNTGIGLA V AAKGYKAV MPETMS ERRNLL+AYGAELVLTPGSE Sbjct: 64 KPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQERRNLLKAYGAELVLTPGSEA 123 Query: 123 MNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXXXX 182 M GA+KKA++ L E YF +QF+N AN ++H TTGPE+++ E G +DAF Sbjct: 124 MKGAIKKAKE-LKEEHGYFEPQQFENPANPEVHELTTGPELLQQFE--GKTIDAFLAGVG 180 Query: 183 XXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNVYD 242 +VLK +YPN++I A+EP ASP+L+GG+PGPHK+QG+GA FIP LN +YD Sbjct: 181 TGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPGPHKLQGLGAGFIPGTLNTEIYD 240 Query: 243 EIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGERY 302 II V ND A E +RRVAKEEG+L+GISSGAA++AA++ AKELG GK VV ++PSNGERY Sbjct: 241 SIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAAIQKAKELGKGKTVVTVLPSNGERY 300 Query: 303 LSTPLYNFE 311 LSTPLY+F+ Sbjct: 301 LSTPLYSFD 309 >BS_ytkP [E] COG0031 Cysteine synthase Length = 311 Score = 349 bits (896), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 171/310 (55%), Positives = 222/310 (71%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 M K+V + DLIG TPLVRLNR+ PE +A++YLKLE+ NP SVKDR A ++ EAE+ G Sbjct: 1 MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LLKPG I+E TSGNTGIGLA+ AAA+GYKA++VMP+TM+ ER NLL+AYGAE+VLTPG Sbjct: 61 LLKPGSVIIEPTSGNTGIGLAMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGE 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 E M G++KKA+++ + P+ ++ QF N AN HR+TT PEI AIE IG PL AF Sbjct: 121 ERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTAPEIARAIEEIGKPLGAFVAS 180 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 E LK +P++ ++ VEPA SP+L+GGKPG HK+ G FIP ILN++V Sbjct: 181 SGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDV 240 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDEII + +++A+ T RR+A EEG+L G SSGAA FAA++ AK L K VV + GE Sbjct: 241 YDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKRLSPDKVVVCMTADTGE 300 Query: 301 RYLSTPLYNF 310 RYLST L++F Sbjct: 301 RYLSTDLWSF 310 >all2521 [E] COG0031 Cysteine synthase Length = 319 Score = 329 bits (844), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 173/312 (55%), Positives = 222/312 (71%), Gaps = 9/312 (2%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGS---AEIYLKLEYQNPGSSVKDRIAISIVEEAEKE 59 ++ N VT+LIGGTPLV+LN++ P+ A I +KLE NP SSVKDRI +S++ AE E Sbjct: 2 RIANDVTELIGGTPLVKLNKI-PQAEGVVARIVVKLEGMNPASSVKDRIGVSMINAAEAE 60 Query: 60 GLLKPGGTI-VEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTP 118 GL+ PG TI VE TSGNTGI LA+VAAA+GY+ ++ MPETMS ERR +LRAYGA L LTP Sbjct: 61 GLIIPGKTILVEPTSGNTGIALAMVAAARGYRLILTMPETMSQERRAMLRAYGATLELTP 120 Query: 119 GSEGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFX 178 G+EGM GA++KAE+I++ PD + +QF+N AN KIHRETT EI + G +D Sbjct: 121 GTEGMRGAIRKAEEIVASTPDTHMLQQFRNPANPKIHRETTAEEIWNDTD---GEVDIVI 177 Query: 179 XXXXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQ 238 EVLK + P+ + AVEP+ SPIL+GG+ GPHKIQGIGA F+PD+L Sbjct: 178 AGVGTGGTITGIAEVLKQRKPSFQAIAVEPSNSPILSGGQAGPHKIQGIGAGFVPDVLRL 237 Query: 239 NVYDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKEL-GAGKKVVVIIPS 297 + DE+I V +D+A RR+A+EEG+LSGISSGAA+ AAL+V K AGK +V+I PS Sbjct: 238 ELVDEVIRVSDDQAMSYGRRLAREEGLLSGISSGAALCAALQVGKRPENAGKLIVMIQPS 297 Query: 298 NGERYLSTPLYN 309 GERYLSTPL+ Sbjct: 298 FGERYLSTPLFQ 309 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,899,786 Number of extensions: 953472 Number of successful extensions: 2277 Number of sequences better than 1.0e-05: 107 Number of HSP's gapped: 2091 Number of HSP's successfully gapped: 107 Length of query: 312 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 206 Effective length of database: 26,153,027 Effective search space: 5387523562 Effective search space used: 5387523562 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 116 (49.3 bits)