BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0009.p (290 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll5833 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase 215 7e-56 mll9102 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase 212 5e-55 mll6272 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase 200 2e-51 ZnadC [H] COG0157 Nicotinate-nucleotide pyrophosphorylase 193 3e-49 >mll5833 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase Length = 293 Score = 215 bits (547), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 124/275 (45%), Positives = 169/275 (61%), Gaps = 4/275 (1%) Query: 13 IKGWLHEDVG-SGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVDPSLVFTA 71 ++ L ED+G +GDIT+ IPA ++ ++A++ G++ G+ + F +VDP + Sbjct: 16 VRSALLEDLGRAGDITSDAIIPADCKATLALNARQAGVVAGLDLVMFAFLLVDPGISIQL 75 Query: 72 LVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLPSRLV 131 EG V+ G IA V G ++LT ER ALN + +LSG+A+ T + V + G +++V Sbjct: 76 RCPEGGKVSAGQTIAIVNGPARSLLTAERTALNFLCKLSGIATATATLVNAVRGHNAKIV 135 Query: 132 DTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARANIPHT 191 TRKTTPG R+LEKYAVR GGGANHRF L DAV+IKDNHI AG IR A+ RAR I H Sbjct: 136 CTRKTTPGLRVLEKYAVRAGGGANHRFALDDAVLIKDNHIAIAGDIRTAIERARRAIGHM 195 Query: 192 MTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNVSLET 251 + I AL AG D ++LDNMS E + +AV + +A TEASG V+ + Sbjct: 196 VKIEVEVDRLDQLEIALTAGVDAVLLDNMSVEDLAQAVAMVGGRA---ITEASGRVTRAS 252 Query: 252 VRRIAETGVDVISVGRLTYSFSSLDISLDLNAKKE 286 IA TGVD+ISVG LT+S LDI LD+ A + Sbjct: 253 APAIAATGVDLISVGWLTHSAPILDIGLDMPADRN 287 >mll9102 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase Length = 284 Score = 212 bits (540), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 119/269 (44%), Positives = 164/269 (60%), Gaps = 4/269 (1%) Query: 13 IKGWLHEDVG-SGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVDPSLVFTA 71 ++ L ED+G +GD+TT +P + ++ A++ G++ G+ +A L F ++D + T Sbjct: 15 VRAALLEDLGRAGDLTTDAIVPKDLRATTVLSARQTGVVAGLDLAILAFRLIDERIETTP 74 Query: 72 LVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLPSRLV 131 + +G VT G IA V G AILT ER ALN + LSG+A+ T S V + +++V Sbjct: 75 VCPDGSEVTPGQTIALVSGPARAILTAERTALNFLSHLSGIATATASIVNAIRDHKAKIV 134 Query: 132 DTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARANIPHT 191 TRKTTPG R +EKYAVR GGG NHRFGL DA++IKDNHI AGGIR A+ R RA++ H Sbjct: 135 CTRKTTPGLRAVEKYAVRAGGGGNHRFGLDDAILIKDNHIAMAGGIRPAIERTRASVGHL 194 Query: 192 MTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNVSLET 251 + I ALA D ++LDNMS + + AV + A TEASG ++ T Sbjct: 195 IKIEVEVDTLAQLEEALALAPDAVLLDNMSLDELRHAVSMV---AGRCITEASGRITTST 251 Query: 252 VRRIAETGVDVISVGRLTYSFSSLDISLD 280 V +A TGVD+IS+G LT+S LDI LD Sbjct: 252 VPAVAATGVDLISIGWLTHSAPILDIGLD 280 >mll6272 [H] COG0157 Nicotinate-nucleotide pyrophosphorylase Length = 292 Score = 200 bits (509), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 119/273 (43%), Positives = 165/273 (60%), Gaps = 4/273 (1%) Query: 13 IKGWLHEDVG-SGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVFEVVDPSLVFTA 71 ++ L ED+G +GD+TT + H ++ ++ A++ GI+ G+ +A L F ++ P + Sbjct: 15 VRAALLEDLGRAGDVTTEAIVTPFHYARTVLAARQRGIVAGLDLAMLAFRLISPDVEILV 74 Query: 72 LVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLPSRLV 131 +G +V +G +IA V G AILT ER ALN + LSG+A+ T S V + P ++ Sbjct: 75 ERPDGCSVVEGEVIASVVGPARAILTAERTALNFLCHLSGIATATASIVNAIRDHPVKIT 134 Query: 132 DTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARANIPHT 191 TRKTTPG R +EKYAVR GGGANHRFGL DA++IKDNHI AGGIR A+ RARA+ H Sbjct: 135 CTRKTTPGLRAVEKYAVRAGGGANHRFGLDDAILIKDNHIAIAGGIRAAIKRARASAGHL 194 Query: 192 MTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNVSLET 251 + I AL D ++LDNMS + + +AV + +A TEASG ++ T Sbjct: 195 VKIEVEVDTLTQLEEALTLTPDAVLLDNMSVDDLRQAVAMVAGRA---ITEASGRITAAT 251 Query: 252 VRRIAETGVDVISVGRLTYSFSSLDISLDLNAK 284 IA T VD+IS+G LT+S LDI LD A Sbjct: 252 APAIAATRVDLISIGWLTHSAPILDIGLDFRAS 284 >ZnadC [H] COG0157 Nicotinate-nucleotide pyrophosphorylase Length = 297 Score = 193 bits (490), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 118/269 (43%), Positives = 155/269 (57%), Gaps = 11/269 (4%) Query: 17 LHEDVG-----SGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVF-EVVDPSLVFT 70 L ED+G + DIT + +P S + +E+G+ CG E VF ++ + Sbjct: 31 LREDLGGTVDANNDITAKL-LPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTII 89 Query: 71 ALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLPSRL 130 V +G + + E+EG + +LTGER ALN +Q LSGVAS+ +V+ LEG ++L Sbjct: 90 WHVDDGDVINANQPLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQL 149 Query: 131 VDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARANIPH 190 +DTRKT PG R KYAV GGGANHR GL DA +IK+NHI +G +RQAV +A P Sbjct: 150 LDTRKTLPGLRSALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPD 209 Query: 191 TMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNVSLE 250 + AL AGADIIMLDN E M EAV+R KA E SGNV+ + Sbjct: 210 A-PVEVEVENLEELDEALKAGADIIMLDNFETEQMREAVKRTNGKA---LLEVSGNVTDK 265 Query: 251 TVRRIAETGVDVISVGRLTYSFSSLDISL 279 T+R AETGVD ISVG LT +LD+S+ Sbjct: 266 TLREFAETGVDFISVGALTKHVQALDLSM 294 Score = 193 bits (490), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 118/269 (43%), Positives = 155/269 (57%), Gaps = 11/269 (4%) Query: 17 LHEDVG-----SGDITTRTTIPAGHESKGIIHAKEDGIICGIPVAELVF-EVVDPSLVFT 70 L ED+G + DIT + +P S + +E+G+ CG E VF ++ + Sbjct: 31 LREDLGGTVDANNDITAKL-LPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTII 89 Query: 71 ALVQEGQAVTKGTIIAEVEGSTHAILTGERLALNLMQRLSGVASRTHSFVQKLEGLPSRL 130 V +G + + E+EG + +LTGER ALN +Q LSGVAS+ +V+ LEG ++L Sbjct: 90 WHVDDGDVINANQPLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQL 149 Query: 131 VDTRKTTPGHRMLEKYAVRVGGGANHRFGLYDAVMIKDNHIKGAGGIRQAVGRARANIPH 190 +DTRKT PG R KYAV GGGANHR GL DA +IK+NHI +G +RQAV +A P Sbjct: 150 LDTRKTLPGLRSALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPD 209 Query: 191 TMTIXXXXXXXXXXXXALAAGADIIMLDNMSPELMTEAVRRIKTKAPHVKTEASGNVSLE 250 + AL AGADIIMLDN E M EAV+R KA E SGNV+ + Sbjct: 210 A-PVEVEVENLEELDEALKAGADIIMLDNFETEQMREAVKRTNGKA---LLEVSGNVTDK 265 Query: 251 TVRRIAETGVDVISVGRLTYSFSSLDISL 279 T+R AETGVD ISVG LT +LD+S+ Sbjct: 266 TLREFAETGVDFISVGALTKHVQALDLSM 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,705,339 Number of extensions: 762367 Number of successful extensions: 11214 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 11198 Number of HSP's successfully gapped: 10 Length of query: 290 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 185 Effective length of database: 26,268,361 Effective search space: 4859646785 Effective search space used: 4859646785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)