BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0001.p (69 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0092 [EH] COG0115 Branched-chain amino acid aminotransferase/4... 66 1e-11 BS_pabC [EH] COG0115 Branched-chain amino acid aminotransferase/... 65 2e-11 >BH0092 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 295 Score = 66.2 bits (160), Expect = 1e-11, Method: Composition-based stats. Identities = 27/50 (54%), Positives = 36/50 (72%) Query: 3 YIGFNHQVVDAKNAVISALDHGFLYGMGLFETFRTYKGEPFLLQRHLDRL 52 Y+ N ++V + A+IS DHG++YG+GLFETFR Y+G PFLL H RL Sbjct: 2 YMYVNDKLVKEEEAIISPFDHGYMYGLGLFETFRLYEGHPFLLDDHFVRL 51 >BS_pabC [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 269 Score = 65.5 bits (158), Expect = 2e-11, Method: Composition-based stats. Identities = 6/49 (12%), Positives = 9/49 (18%) Query: 4 IGFNHQVVDAKNAVISALDHGFLYGMGLFETFRTYKGEPFLLQRHLDRL 52 N ++ A G +QR D Sbjct: 2 FLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQRLQDAC 50 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.148 0.462 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 5,071,882 Number of extensions: 145616 Number of successful extensions: 444 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 451 Number of HSP's successfully gapped: 2 Length of query: 69 Length of database: 38,378,431 Length adjustment: 41 Effective length of query: 28 Effective length of database: 33,649,737 Effective search space: 942192636 Effective search space used: 942192636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 109 (46.6 bits)