BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0057.0015.p (297 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3547_2 [G] COG0524 Sugar kinases, ribokinase family 197 2e-50 PA1950 [G] COG0524 Sugar kinases, ribokinase family 194 2e-49 HI0505 [G] COG0524 Sugar kinases, ribokinase family 185 9e-47 VCA0131 [G] COG0524 Sugar kinases, ribokinase family 182 4e-46 all1784 [G] COG0524 Sugar kinases, ribokinase family 179 4e-45 >STM3547_2 [G] COG0524 Sugar kinases, ribokinase family Length = 312 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 109/297 (36%), Positives = 165/297 (55%), Gaps = 5/297 (1%) Query: 1 MDMVVRTSRAPNAGETLFGQGFALSPXXXXXXXXXXXXXXXXDVQMIGRVGRDTFGSELL 60 +D++ R P+ GE+L F SP DV I +VG D F + Sbjct: 10 VDIISYLPRLPSTGESLLADKFIFSPGGKGCNQALAASYADSDVHFITKVGSDHFSDYAI 69 Query: 61 EVMRKEQINTEHIGQSETQTTGVASIVVDGE-GENRIIVVPGANIEMGPADIAALEPVIS 119 + +I+ I Q++ TG A+I+V+G+ G+N I + PGAN+ + P +I + I Sbjct: 70 NFINSSKIHKSVIYQTKETQTGTATIMVNGDTGDNVIAIYPGANMTISPDEITIQKEAIV 129 Query: 120 QAEIVVMQLETDLXXXXXXXXXXXXXGIPVILNPAPARELDDEFLQHVTYLTPNETEAGI 179 ++IV++QLET+ IPVI+NPAP ++ + + ++ Y+TPNETEAG+ Sbjct: 130 HSDIVLVQLETNYEALQQTIRLAQKNDIPVIINPAPYNDMVNTIIDNIDYITPNETEAGL 189 Query: 180 LAGIAVNSVAEAEQAAHILLQKGVQNVIVTLGSKGALIVNKGGTLHIPGFPVQAVDTVAA 239 LA +AVN + A+ AA I+ QKGV+N I+TLGSKG+L + ++ P FP +T A Sbjct: 190 LANMAVNDIESAKCAAKIIHQKGVKNTIITLGSKGSLAYDGTQFIYSPAFPAVVKNTAGA 249 Query: 240 GDSFNGALAWQLTQGKTLAEAARFANAVGALAVGKQGAIPSLPYLPEVEQFLQDAAG 296 GD+FNGALA L +GK+LA A +A+A +LAV P+ +PE + L G Sbjct: 250 GDAFNGALASGLAKGKSLASALCYASAFASLAV----ETPNASDMPENDSVLHRIQG 302 >PA1950 [G] COG0524 Sugar kinases, ribokinase family Length = 308 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 118/292 (40%), Positives = 165/292 (56%), Gaps = 1/292 (0%) Query: 1 MDMVVRTSRAPNAGETLFGQGFALSPXXXXXXXXXXXXXXXXDVQMIGRVGRDTFGSELL 60 MD+VVR R P GETL GQ F P +V MIG +G D +G +L Sbjct: 13 MDLVVRAPRLPRGGETLAGQSFITVPGGKGANQAVAAARLGAEVAMIGCLGDDAYGDQLY 72 Query: 61 EVMRKEQINTEHIGQSETQTTGVASIVVDGEGENRIIVVPGANIEMGPADIAALEPVISQ 120 ++ E I+ + + + +++GVA IVVD +N I++V G N + PA +A E ++ Q Sbjct: 73 RALQAEGIDCQGVERVAGESSGVALIVVDDSSQNAIVIVAGGNGHLSPAVLARHEHLLEQ 132 Query: 121 AEIVVMQLETDLXXXXXXXXXXXXXGIPVILNPAPA-RELDDEFLQHVTYLTPNETEAGI 179 A++VV QLE+ L G VILNPAPA R++ E+L V YL PNETE+ + Sbjct: 133 AQVVVCQLESPLETVGHVLRRAHALGKTVILNPAPATRDVPAEWLPLVDYLVPNETESEL 192 Query: 180 LAGIAVNSVAEAEQAAHILLQKGVQNVIVTLGSKGALIVNKGGTLHIPGFPVQAVDTVAA 239 L + V+S+ A +AA L + G VIVTLG++GAL+V +G H P V+A+DT AA Sbjct: 193 LCRLPVDSLESAGRAAERLREMGAGRVIVTLGAQGALLVGEGRVEHFPVARVKALDTTAA 252 Query: 240 GDSFNGALAWQLTQGKTLAEAARFANAVGALAVGKQGAIPSLPYLPEVEQFL 291 GD+F G A L +G A A RF A A++V + GA S+P EVE+ L Sbjct: 253 GDTFVGGFAAALARGLDEAAAIRFGQAAAAISVTRLGAQTSIPSREEVERAL 304 >HI0505 [G] COG0524 Sugar kinases, ribokinase family Length = 306 Score = 185 bits (469), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 107/293 (36%), Positives = 152/293 (51%) Query: 2 DMVVRTSRAPNAGETLFGQGFALSPXXXXXXXXXXXXXXXXDVQMIGRVGRDTFGSELLE 61 D V+ GETL GQ + ++ V I +G D+ G + Sbjct: 14 DHVISVPYFTKPGETLTGQNYQIAYGGKGANQAVAAARLGAKVAFISCIGSDSIGKTMKN 73 Query: 62 VMRKEQINTEHIGQSETQTTGVASIVVDGEGENRIIVVPGANIEMGPADIAALEPVISQA 121 +E I+T HI + TG+A I V EN I++ GAN + + E I+Q+ Sbjct: 74 AFAQEGIDTTHINTVSQEMTGMAFIQVAKSSENSIVLASGANSHLSEMVVRQSEAQIAQS 133 Query: 122 EIVVMQLETDLXXXXXXXXXXXXXGIPVILNPAPARELDDEFLQHVTYLTPNETEAGILA 181 + ++MQLET L G+ V+LNPAPA+ L DE L + +TPNETEA IL Sbjct: 134 DCLLMQLETPLSGVELAAQIAKKNGVKVVLNPAPAQILSDELLSLIDIITPNETEAEILT 193 Query: 182 GIAVNSVAEAEQAAHILLQKGVQNVIVTLGSKGALIVNKGGTLHIPGFPVQAVDTVAAGD 241 G+ V A +AA + KG++ V++TLG+KG + KG + I GF VQA+DT AAGD Sbjct: 194 GVEVADEQSAVKAASVFHDKGIETVMITLGAKGVFVSRKGKSRIIKGFCVQAIDTTAAGD 253 Query: 242 SFNGALAWQLTQGKTLAEAARFANAVGALAVGKQGAIPSLPYLPEVEQFLQDA 294 +FNG L + K+ EA RF A A++V K+GA S+P E +FL+ A Sbjct: 254 TFNGGFVTALLEEKSFDEAIRFGQAAAAISVTKKGAQSSIPTRQETLEFLEHA 306 >VCA0131 [G] COG0524 Sugar kinases, ribokinase family Length = 306 Score = 182 bits (463), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 119/290 (41%), Positives = 152/290 (52%) Query: 2 DMVVRTSRAPNAGETLFGQGFALSPXXXXXXXXXXXXXXXXDVQMIGRVGRDTFGSELLE 61 D V++ P GETL G+ + + P DV I VG D+FG + E Sbjct: 13 DHVLQVPSFPRPGETLHGRNYQVIPGGKGANQAVAAARMQADVGFIACVGDDSFGINIRE 72 Query: 62 VMRKEQINTEHIGQSETQTTGVASIVVDGEGENRIIVVPGANIEMGPADIAALEPVISQA 121 + + INT + TG+A I V GEN I + AN ++ A I I A Sbjct: 73 SFKLDGINTAGVKLQPNCPTGIAMIQVSDSGENSICISAEANAKLTAAAIEPDLAAIRDA 132 Query: 122 EIVVMQLETDLXXXXXXXXXXXXXGIPVILNPAPARELDDEFLQHVTYLTPNETEAGILA 181 ++MQLET L VILNPAPAREL DE L+ V +TPNETEA +L Sbjct: 133 RYLLMQLETPLDGILKAAQEAKTAKTNVILNPAPARELPDELLKCVDLITPNETEAEVLT 192 Query: 182 GIAVNSVAEAEQAAHILLQKGVQNVIVTLGSKGALIVNKGGTLHIPGFPVQAVDTVAAGD 241 GI V + A+QAA L KG++ VI+TLGSKG + G IPGF V+A DT AAGD Sbjct: 193 GITVYDDSSAQQAADALHCKGIEIVIITLGSKGVWLSQNGRGQRIPGFVVKATDTTAAGD 252 Query: 242 SFNGALAWQLTQGKTLAEAARFANAVGALAVGKQGAIPSLPYLPEVEQFL 291 +FNGAL L Q L A +FA+A A++V + GA S+P EVE FL Sbjct: 253 TFNGALVTGLLQEMPLESAIKFAHAAAAISVTRFGAQTSIPTRAEVEAFL 302 >all1784 [G] COG0524 Sugar kinases, ribokinase family Length = 307 Score = 179 bits (455), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 115/294 (39%), Positives = 160/294 (54%), Gaps = 1/294 (0%) Query: 1 MDMVVRTSRAPNAGETLFGQGFALSPXXXXXXXXXXXXXXXXDVQMIGRVGRDTFGSELL 60 +D+V + P AGETL G+ F P QM+GRVG +FG+EL+ Sbjct: 11 IDLVATAPKLPGAGETLLGEDFFKVPGGKGANQAVALARLGVPTQMVGRVGAQSFGAELM 70 Query: 61 EVMRKEQINTEHIGQSETQTTGVASIVVDGEGENRIIVVPGANIEMGPADIAALEPVISQ 120 + ++ + T++I ET ++GVA I V GEN+I+V+PGAN + D+ L ++ Sbjct: 71 QNLQDAGVQTDNIFVDETASSGVAMITVAHNGENQIVVIPGANGRVNQVDVERLSRILPT 130 Query: 121 AEIVVMQLETDLXXXXXXXXXXXXXGIPVILNPAPA-RELDDEFLQHVTYLTPNETEAGI 179 A +++QLE + I VIL+PAPA +L E V +TPNE EAG Sbjct: 131 ATALLLQLEIPVPAVIAAAQAARSNNITVILDPAPAPSDLPTELYSFVDIITPNEVEAGQ 190 Query: 180 LAGIAVNSVAEAEQAAHILLQKGVQNVIVTLGSKGALIVNKGGTLHIPGFPVQAVDTVAA 239 L G V+ A +AA +LLQKGV++ IV LG+KG + T +P F V +DTVAA Sbjct: 191 LVGFPVDGEDAARKAAGVLLQKGVKSAIVKLGAKGVVCATNKETFFVPAFSVDTLDTVAA 250 Query: 240 GDSFNGALAWQLTQGKTLAEAARFANAVGALAVGKQGAIPSLPYLPEVEQFLQD 293 GD+FNG LA L G +L +A + A GALA K GA SLP E FL++ Sbjct: 251 GDAFNGGLATALYSGLSLHQAVIWGAAAGALAATKLGAQTSLPDKLTFEAFLKE 304 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,515,225 Number of extensions: 749508 Number of successful extensions: 1982 Number of sequences better than 1.0e-05: 103 Number of HSP's gapped: 1908 Number of HSP's successfully gapped: 103 Length of query: 297 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 191 Effective length of database: 26,153,027 Effective search space: 4995228157 Effective search space used: 4995228157 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)