BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0057.0014.p
         (254 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ML0062 [ER] COG0493 NADPH-dependent glutamate synthase beta chai...   104   1e-22

>ML0062 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and
           related oxidoreductases
          Length = 252

 Score =  104 bits (259), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 9/211 (4%), Positives = 37/211 (17%), Gaps = 16/211 (7%)

Query: 44  DLVEWRSDFFAEVEDLAAVRKTLAEISSVLPDTPLIFTFRSAREGGEREITTEYYIRLNQ 103
           + VE+ +D      +  A+ +     +        I +  +        +    ++  + 
Sbjct: 30  ENVEYVADLGCGPGNSTALLQQRWPAA----RITGIDSSPAMIAEARSALPDCQFVEADI 85

Query: 104 A--AAESGYVDIIDVELFNEENVVRELVAAAHACGVFVIVSNHDFHGTPSQEEIVSRLRQ 161
                         +        +       +     ++   +         ++     +
Sbjct: 86  RNWQPVQAL---DLIFANASLQWL----PDHYELFPHLVSLLNPQGVLAV--QMPDNWLE 136

Query: 162 AQALGGDLPKIAVMPQQAGDVLTLLAATNTMREQYADRPIITMSMAGEGVISRLAGEIFG 221
              +              G                A    + +              I  
Sbjct: 137 PTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGC-EVDIWRTTYYHQMPSHQAIID 195

Query: 222 SALTFGAAHKPSAPGQVAVAELRRVLELLHR 252
                G         +       +    +  
Sbjct: 196 WVTATGLRPWLQDLTESEQQLFLKRYHQMLE 226


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.318    0.134    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 17,295,907
Number of extensions: 694429
Number of successful extensions: 1322
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 1319
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 38,378,431
Length adjustment: 104
Effective length of query: 150
Effective length of database: 26,383,695
Effective search space: 3957554250
Effective search space used: 3957554250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 114 (48.5 bits)