BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0057.0001.p (847 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc04255 [G] COG3250 Beta-galactosidase/beta-glucuronidase 464 e-130 AGc2809 [G] COG3250 Beta-galactosidase/beta-glucuronidase 457 e-128 TM1624 [G] COG3250 Beta-galactosidase/beta-glucuronidase 408 e-113 AGc4665 [G] COG3250 Beta-galactosidase/beta-glucuronidase 189 2e-47 >SMc04255 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 831 Score = 464 bits (1193), Expect = e-130, Method: Compositional matrix adjust. Identities = 287/818 (35%), Positives = 413/818 (50%), Gaps = 48/818 (5%) Query: 9 NWQFRACGDEQWLPAAVPGTVHTDLLCNELIPSPFYGKNEHELQWIDKKDWEY-RTVL-- 65 +W A + L A+PG VH+ L +I P+ G+NE ++QW+ +KDW RTV+ Sbjct: 25 DWLLAAADNSHALTIALPGDVHSALQRAGIIADPYRGRNEADVQWVARKDWVLERTVVID 84 Query: 66 --QLGGEWQQRAVAELVFKGLDTYADVYVNDVHALSADNMFRAWTVDVKELLRAGENEIR 123 L G W L F+ +DT A VYVND L A+N FR + DV + AGEN IR Sbjct: 85 ADDLNGGWY------LDFESIDTVASVYVNDELVLQAENCFRRYRPDVSGAIVAGENRIR 138 Query: 124 VRFRSAVHEDLPKLEQLGYALPAPNDQSALGGLGEQQISVFARKAPYHYGWDWGPRFLTS 183 + SA+ E + + +P + S + RK H+GWDW Sbjct: 139 IVLHSAIAEGARRQAAQPFYVPYHDGNSPIAN------GNMLRKPQCHFGWDWNIAIAPL 192 Query: 184 GIWREAVLEGRDQVAITDLYIRQNAVNPEEARLTVLVEADAPSAWNGLLRVSCGGREWTQ 243 GI+ L + I + RQ ++ L V V+ A + G Sbjct: 193 GIYGSLALCRLETARIEHVTTRQMWLDDGSVDLQVTVQLFAREPGIVPVHFMLDGVRERL 252 Query: 244 SVSLQQGKQRLELEIVLDQPRLWWCNGLGKPELTAFRAELLKAGEAAAQAEVTTGLREIK 303 ++ G+ RL + +PR WW G G + + E+ GE + G R I+ Sbjct: 253 DCAVGAGETRLTHVFTVQEPRRWWPAGSGDQAFSTLKVEV--PGETVTR---QIGFRTIE 307 Query: 304 LIREPDDRGASFYFELNGVRVFAKGSNHIPNDSFSTEVTEERYRHEIASAVASNMNMLRV 363 L+ + D+ G+ F F +NG +F +G+N IP D+ + VT E + SAV +NMNM+RV Sbjct: 308 LVTDKDEAGSRFAFRVNGREIFCRGANWIPADALMSRVTPESVEDLLRSAVDANMNMIRV 367 Query: 364 WGGGIYEEETFYRLCDEYGLLVWQDFMFACSMYPGDEPFLENVREEAVYNIRRLRNHPCI 423 WGGG YE + FY LCD GLLVWQDFMFAC++YP FLENV E Y +RRL HP + Sbjct: 368 WGGGFYEPDWFYDLCDRLGLLVWQDFMFACNLYPSTPDFLENVAAEVDYQVRRLSTHPSL 427 Query: 424 ALWCGNNEMDSAWSHYEDNKGWGWKEKFSREIR-EKLWKDYEAVFHRILPEAVASCHPDV 482 ALWCG+NE+ A + +E+++ +++ + ++L + EAV + PEA+ Sbjct: 428 ALWCGDNELVGALTWFEESR----RDRDRYLVSYDRLNRTVEAVMKQACPEAI------- 476 Query: 483 DYWASSP-MRALTHDMNQHATRMAGEGDVHYWGVWHGSEPFENYNIKVGRFMSEYGFQSF 541 +W SSP + L HA G GD+HYW VWH ++ F+NY RF SE+GFQS+ Sbjct: 477 -WWPSSPSVGYLDFGDAWHAD---GAGDMHYWSVWHENKSFDNYRTVRPRFCSEFGFQSY 532 Query: 542 PELKSVLSYAEENDMELESEVMLAHQKNGRGNLLIKEYMDIYLPRPKDFKSFLYMSQLLQ 601 ++ + +AE +D+ + S VM AHQKN GN I M Y PKDF SF+Y+SQ+ Q Sbjct: 533 TSMQVIRQFAEAHDLNIASPVMEAHQKNAGGNERIAGTMFRYFRFPKDFPSFVYLSQIQQ 592 Query: 602 AEAMRIAIESHRRNKPYCMGTLYWQMNDCWPVASWAGMDYYGRWKALQYTVRKSYQEL-L 660 A+R A++ R KP+CMGTLYWQ+ND WPVASW+ +DY G WKA+ Y R+ +Q + + Sbjct: 593 GLAIRTAVDYWRSLKPHCMGTLYWQLNDTWPVASWSSLDYGGHWKAMHYMARRFFQPVAV 652 Query: 661 LSIDGSDGENLQIHAVSDRQEGLEGELRVRLQDFSGAVLQEWSQPVALAADSASIVFAAN 720 +I +DG + V+D E EL+ L G + + AD AS + Sbjct: 653 AAIPSADGREIAFSMVNDTAEPATVELQAFLVSLDGNRQRLLAAAGTCPADRASTLVTVA 712 Query: 721 IAGLLEGREPRRVXXXXXXXXXXXXXXXKHHYFVSAKEIPLARPAVKVEEV---QGSAAF 777 + P H+ + K + L + +E V GS Sbjct: 713 AKDI-----PPDTLLFWSFEASNGATGEGHYAHGTYKALDLVPSGLTIEAVPRPDGSFDV 767 Query: 778 TVSTDVLARGVYLTADEDGIFSDNFFDLLPGEAKTVEF 815 V+ LA V + AD +G +SDN FDL GE K++ F Sbjct: 768 AVTAAGLALHVMIEADVEGRYSDNAFDLTAGETKSIRF 805 >AGc2809 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 218 Score = 457 bits (1175), Expect = e-128, Method: Compositional matrix adjust. Identities = 0/820 (0%), Positives = 0/820 (0%), Gaps = 41/820 (5%) Query: 10 WQFRACGDEQWLPAAVPGTVHTDLLCNELIPSPFYGKNEHELQWIDKKDWEYRTVLQLGG 69 + + T I + ++ + Sbjct: 22 QELILASSSASRQMLMRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKAL- 80 Query: 70 EWQQRAVAELVFKGLDTYADVYVNDVHALSADNMFRAWTVDVKELLRAGENEIRVRFRSA 129 A+ V+ + A + N V + Sbjct: 81 --AVSALHPEALVLGCDQTMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNG 138 Query: 130 VHEDLPKLEQLGYALPAPNDQSALGGLGEQQISVFARKAPYHYGWDWGPRFLTSGIWREA 189 + + KA G R Sbjct: 139 KVVWQTVSSAELAVRTLSAEFVSRHL------QRVGEKALSSVGAYQLEREGIQLFTSIE 192 Query: 190 VLEGRDQVAITDLYIRQNAV-NPEEARLTVLVEADAPSAWNGLLRVSCGGREWTQSVSLQ 248 + + Sbjct: 193 GDYFTILGLPLLPLLSKLRDM-- 250 Query: 249 QGKQRLELEIVLDQPRLWWCNGLGKPELTAFRAELLKAGEAAAQAEVTTGLREIKLIREP 308 Sbjct: 251 ----- 305 Query: 309 DDRGASFYFELNGVRVFAKGSNHIPNDSFSTEVTEERYRHEIASAVASNMNMLRVWGGGI 368 Sbjct: 306 365 Query: 369 YEEETFYRLCDEYGLLVWQDFMFACSMYPGDEPFLENVREEAVYNIRRLRNHPCIALWCG 428 Sbjct: 366 425 Query: 429 NNEMDSAWSHYEDNKGWGWKEKFSREIREKLWKDYEAVFHRILPEAVASCHPDVDYWASS 488 Sbjct: 426 ----------- 474 Query: 489 PMRA-LTHDMNQHATRMAGEGDVHYWGVWHGSEPFENYNIKVGRFMSEYGFQSFPELKSV 547 Sbjct: 475 --- 531 Query: 548 LSYAEENDMELESEVMLAHQKNGRGNLLIKEYMDIYLPRPKDFKSFLYMSQLLQAEAMRI 607 Sbjct: 532 591 Query: 608 AIESHRRNKPYCMGTLYWQMNDCWPVASWAGMDYYGRWKALQYTVRKSYQELLLS-IDGS 666 Sbjct: 592 651 Query: 667 DGENLQIHAVSDRQEGLEGELRVRLQDFSGAVLQEWSQPVALAADSASIVFAANIAGLLE 726 Sbjct: 652 711 Query: 727 GREPRRVXXXXXXXXXXXXXXXKHHYFVSAKEIPLARPAVKVEE---VQGSAAFTVSTDV 783 Sbjct: 712 ----- 766 Query: 784 LARGVYLTADEDGIFSDNFFDLLPGEAKTVEFFLSGKGEQ 823 Sbjct: 767 806 >TM1624 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 785 Score = 408 bits (1049), Expect = e-113, Method: Compositional matrix adjust. Identities = 265/797 (33%), Positives = 404/797 (50%), Gaps = 55/797 (6%) Query: 23 AAVPGTVHTDLLCNELIPSPFYGKNEHELQWIDKKDWEYRTVLQLGGEWQQRAVAELVFK 82 VPG V DL+ L+P P+ G NE + I+ ++W Y + + ++ +LVF+ Sbjct: 23 GTVPGVVQADLVRKGLLPHPYVGMNEDLFKEIEDREWIYEREFEFKEDVKEGERVDLVFE 82 Query: 83 GLDTYADVYVNDVHALSADNMFRAWTVDVKELLRAGENEIRVRFRSAVHEDLPKLEQLGY 142 G+DT +DVY+N V+ S ++MF + DV +L+ +N ++V +S + +PK + Y Sbjct: 83 GVDTLSDVYLNGVYLGSTEDMFIEYRFDVTNVLKE-KNHLKVYIKSPIR--VPKTLEQNY 139 Query: 143 ALPAPNDQSALGGLGEQQISVFARKAPYHYGWDWGPRFLTSGIWREAVLEG-RDQVAITD 201 + LGG E I + RKA Y YGWDWG R +TSGIW+ LE R ++ + Sbjct: 140 GV--------LGG-PEDPIRGYIRKAQYSYGWDWGARIVTSGIWKPVYLEVYRARLQDST 190 Query: 202 LYIRQNAVNPEEARLTVLVEADAPSAWNGLLRVSCGGREWTQSVSLQQGKQRLELEIV-L 260 Y+ + R+ V + N ++ V G + + L++ ++L + L Sbjct: 191 AYLLELEGKDALVRVNGFVHGEG----NLIVEVYVNGEKIGEFPVLEKNGEKLFDGVFHL 246 Query: 261 DQPRLWWCNGLGKPELTAFRAELLKAGEAAAQAEVTTGLREIKLIREPDDRGASFYFELN 320 +LW+ +GKP L F L + E GLR +++++EPD+ G +F FE+N Sbjct: 247 KDVKLWYPWNVGKPYLYDFVFVLKDLNGEIYREEKKIGLRRVRIVQEPDEEGKTFIFEIN 306 Query: 321 GVRVFAKGSNHIPNDSFSTEVTEERYRHEIASAVASNMNMLRVWGGGIYEEETFYRLCDE 380 G +VFAKG+N IP+++ T + EE Y + A ++NMNMLRVWGGGIYE E FYRLCDE Sbjct: 307 GEKVFAKGANWIPSENILTWLKEEDYEKLVKMARSANMNMLRVWGGGIYEREIFYRLCDE 366 Query: 381 YGLLVWQDFMFACSMYPGDEPFLENV-REEAVYNIRRLRNHPCIALWCGNNEMDSAWSHY 439 G++VWQDFM+AC YP P+ + EEA +R+LR HP I LWCGNNE Sbjct: 367 LGIMVWQDFMYACLEYPDHLPWFRKLANEEARKIVRKLRYHPSIVLWCGNNE-------- 418 Query: 440 EDNKGWGWKE--KFSREIREKLWKDYEAVFHRILPEAVASCHPDVDYWASSPMRALTHDM 497 WG+ E +R++ + +F PE A P YW SSP + Sbjct: 419 ---NNWGFDEWGNMARKVDGINLGNRLYLFD--FPEICAEEDPSTPYWPSSPYGGEKANS 473 Query: 498 NQHATRMAGEGDVHYWGVWHGSEPFENYNIKVGRFMSEYGFQSFPELKSVLSYAEENDME 557 + EGD H W VW G +ENY GRF+SE+GFQ P +++ +++ + E Sbjct: 474 EK-------EGDRHVWYVWSGWMNYENYEKDTGRFISEFGFQGAPHPETIEFFSKPEERE 526 Query: 558 LESEVMLAHQKNGRGNLLIKEYMDIYLPRPKDFKSFLYMSQLLQAEAMRIAIESHRRNKP 617 + VML H K G + ++ + KDF SF+Y+SQL QAEA++ +E R K Sbjct: 527 IFHPVMLKHNKQVEGQERLIRFIFGNFGKCKDFDSFVYLSQLNQAEAIKFGVEHWRSRKY 586 Query: 618 YCMGTLYWQMNDCWPVASWAGMDYYGRWKALQYTVRKSYQELLLSIDGSDGENLQIHAVS 677 G L+WQ ND WPV SW+ +DY+ R KAL Y R+ + E+L + D + +++ VS Sbjct: 587 KTAGALFWQFNDSWPVFSWSAVDYFKRPKALYYYARRFFAEVLPVLKKRDNK-IELLVVS 645 Query: 678 DRQEGLEGELRVRLQDFSGAVLQEWSQPVALAADSASIVFAANIAGLLEGREPRRVXXXX 737 D +E E LR+ + G + E + L AD S+V + L Sbjct: 646 DLRETKEVSLRLAAYEKKGEKVFEKTYRTVLPADGVSLVDRVELPDDL---------FFF 696 Query: 738 XXXXXXXXXXXKHHYFVSAKEIPLARPAVKVEEVQGSAAFTVSTDVLARGVYLTADEDGI 797 + F +++ L P + +E++ T A GV + +DE + Sbjct: 697 VEAEVDGVKYRNYRVFRKWRDLELPDPEISLEDMGELIELTAQRP--AFGVKILSDE--V 752 Query: 798 FSDNFFDLLPGEAKTVE 814 D+F L PG + T++ Sbjct: 753 PEDDFLFLEPGRSITLK 769 >AGc4665 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 466 Score = 189 bits (479), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 0/815 (0%), Positives = 0/815 (0%), Gaps = 103/815 (12%) Query: 54 IDKKDWEYRTVLQLGGEWQQRAVAELVFKGLDTYADVYVNDVHALSADNMFRAWTVDVKE 113 K+ + + + Sbjct: 66 TLKELGAEI--RWASCNIFSTQD--HAAAAIAAAGIPVFAVKGESLTEYWEYTDKIFQW- 120 Query: 114 LLRAGENEIRVRFRSAVHEDLPKLEQLGYALPAPNDQSALGGLGEQQISVFARKAPYHYG 173 G + + A + S + + Sbjct: 121 --TDGGLSNMILDDGGD----ATMYI-----LLGARAEAGEDVLSNPGSEE-EEILFAQI 168 Query: 174 WDWGPRFLTSGIWREAVLEGRDQVAITDLYIRQNAVNPEEARLTVLVEA--DAPSAWNGL 231 + L+G + T + + L + Sbjct: 169 NKRLKASPGWFTKQRDALKGVTEETTT---GVHRLYDLAKKGLLPFPAINVNDSVT---- 221 Query: 232 LRVSCGGREWTQSVSLQQGKQ-RLELEIVLDQPRLWWCNGLGKPELTAFRAELLKAGEAA 290 + + + V+ ++ G G + + A Sbjct: 222 -KSKFDN----KYGCKESLVDGIRRATDVMMAGKVAVVCGYGDVGKGSAASLQ----GAG 272 Query: 291 AQAEVTTGLREIKLIREPDDRGASFYFELNGVRVFAKGSNHIPNDSFSTEVTEERYRHEI 350 A+ +VT L D + V N + Sbjct: 273 ARVKVTEIDPICALQAAMDGFE--VVRLEDVVSSADIFITTTGNKDVIRIEHMREMKDMA 330 Query: 351 ASAVASNMNMLRVWGGGIYEEETFYRLCDEYGLLVWQDFMFACSMYPGDEPFLENVREEA 410 + + + T + + + + S Sbjct: 331 IVGNIGHFDNEIQVASLRNLKWTNIKPQVDMIEFPKGNRIILLSEGRLLN---LGNATGH 387 Query: 411 VYNIRRLRN----HPCIALWCGNNEMDSAWSHYEDNKGWGWKEKFSREIREKLWKDYEAV 466 + I L+ E + + Sbjct: 388 PSFVMSASFTNQVLGQIELFTKPGEY------KNEVYVLP--------KHLDEKVA--RL 431 Query: 467 FHRILPEAVASCHPDVDYWASSPM-RALTHDMNQHATRMAGEGDVHYWGVWHGSEPFENY 525 L + + + Sbjct: 432 HLEKLGVRLTELSDLQADYIGISKQGPFKAEHY-------- 483 Query: 526 NIKVGRFMSE-YGFQSFPELKSVLSYAEENDMELESEVMLAHQKNGRG------------ 572 Sbjct: 484 -- 541 Query: 573 -NLLIKEYMDIYLPRPKDFKSFLYMSQLLQAEAMRIAIESHRRNKPYCMGTLYWQMNDCW 631 Sbjct: 542 -- 599 Query: 632 PVASWAGMDYYGRWKALQYTVRKSYQELLLSIDGSDGENLQIHAVSDRQEGLEGELRVRL 691 Sbjct: 600 659 Query: 692 QDFSGAVLQEWSQPVALAADSASIVFAANIAGLL--EGREPRRVXXXXXXXXXXXXXXXK 749 Sbjct: 660 719 Query: 750 HHYFVSAKEIPLARPA--------VKVEEVQGSAAFTVSTDVLARGVYLTADEDGIFSDN 801 Sbjct: 720 - 778 Query: 802 FFDLLPGEAKTVEFFLSGKGEQDFTPAAPKGLEIS 836 Sbjct: 779 ---- 809 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 64,932,130 Number of extensions: 2757855 Number of successful extensions: 5114 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 5092 Number of HSP's successfully gapped: 8 Length of query: 847 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 732 Effective length of database: 25,115,021 Effective search space: 18384195372 Effective search space used: 18384195372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)