BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0056.0002.p (431 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0293 [GEPR] COG0477 Permeases of the major facilitator superfa... 80 9e-15 TM0342 [GEPR] COG0477 Permeases of the major facilitator superfa... 75 2e-13 FN1168 [GEPR] COG0477 Permeases of the major facilitator superfa... 69 2e-11 MA1161 [GEPR] COG0477 Permeases of the major facilitator superfa... 67 8e-11 NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe ... 67 8e-11 >BH0293 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 79.7 bits (195), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 89/390 (22%), Positives = 160/390 (41%), Gaps = 15/390 (3%) Query: 24 KSRAFPFLWLGHLISFLGSSVTMVILPVLVYSLTGSTTTMGVVMAVYMLPNILMLPVSGH 83 +++ F LW+ + S L S+ + V G T +GVV+ +P +L++ + G Sbjct: 22 RNQKFLLLWVASIFSGLSFSIYFLTKSWYVVDELGLKTFLGVVLMAMTVPRVLLMMIGGV 81 Query: 84 IVDRYDRVGIMMLADIFRALIMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQPAYAAV 143 + DR+ R IM + I R++++ F PA A+ Sbjct: 82 LADRFRRSTIMFCSLITRSVLLFGMFILQFLGVINIWTLIGFAFCIGILDAFFWPANYAI 141 Query: 144 RATVFTPDIRVTANSVTQITTQTVRLIGPALGGLLITHLSAGIGFGLDAFTYVF-SFICL 202 ++ D ANS Q T +IGP + G LIT + A+ ++F + +CL Sbjct: 142 LPSIVKKDHLTRANSFLQSTNHLTLIIGPMIAGWLIT---------ITAYEWIFVTIMCL 192 Query: 203 IYLRRALIARVRTDASGTAGSMAAPSPSADWKQDFMEGLAVLRSHPWLWITILAFSFINI 262 + + LI RV+ + + PSP +D EG + LR P L + F+N Sbjct: 193 LIIGAILIFRVK-EPFDKPETKRLPSP----LKDLTEGYSYLRHSPVLLSLMWMSVFVNF 247 Query: 263 CYGGITSILVPWLFKVQHGWDPYLYGLAVTCSXXXXXXXXXXFGTRQRWSRRGLMAYGGA 322 G ++ +P + + + DP + G S+RGL+ Sbjct: 248 FITGPGTMAIPLIVEERLKGDPLELSYLESTIAGGLILGAIMIGIINLRSKRGLLIIIRL 307 Query: 323 FISGVSLLLLPFVPSAAGAVILFSLEGFGLMVFMLIWEISMQELVPQEAFGRVASLDLLG 382 + GV++ LL V S A+ + ++ G G+ + + +QE + GRV + Sbjct: 308 TVMGVAIALLGQVVSLWQAIFVLAILGMGISIGDIPARAVVQEKTDFDKIGRVMGMMSTA 367 Query: 383 SFALLPVGYILVGWLADLIGGVATIAIFSG 412 S L+P+ Y + L +++I ++ G Sbjct: 368 SSGLIPLSYGMTSVALSLNLSISSILLYCG 397 >TM0342 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 413 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 88/397 (22%), Positives = 158/397 (39%), Gaps = 46/397 (11%) Query: 25 SRAFPFLWLGHLISFLGSSVTMVILPVLVYSLTGSTTTMGVVMAVYMLPNILMLPVSGHI 84 R F L LG +S LGSSV V +P+ V LT S + + ++ YM+P IL P++G + Sbjct: 8 KRNFFLLALGRFVSILGSSVQAVAIPLFVLDLTHSGSAVALMTVAYMVPRILFTPIAGIV 67 Query: 85 VDRYDRVGIMMLADIFRALIMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQPAYAAVR 144 DR DR +M+ D R ++ F A+ Sbjct: 68 GDRGDRKFLMVSMDFLRGFLICFLALLAFRNSITLPVLYVSQIAMAIMDNLFDIPTGAMF 127 Query: 145 ATVFTPDIRVTANSVTQITTQTVRLIGPALGGLL-------ITHLSAGIGFGLDAFTYVF 197 + D + ANS+ + +++GPALGG++ I L G+ F L A + +F Sbjct: 128 PDIVPQDFLMRANSIMGMVRIFPQILGPALGGVIYGFYGIAIVFLVNGVSFVLSAISEIF 187 Query: 198 SFICLIYLRRALIARVRTDASGTAGSMAAPSPSADWKQDFMEGLAVLRSHPWLWITILAF 257 ++Y R+ P+ + +D EG + +H + TIL F Sbjct: 188 ----IVYSRKV---------------EKKPAKFSQILEDLKEGFLFIWNHELIR-TILIF 227 Query: 258 S-FINICYGGITSILVPWLFKVQHGWDPYLYGLAVTC----SXXXXXXXXXXFGTRQRWS 312 + F+N+ + ++ P+ + + +G T + + W Sbjct: 228 ALFLNLLISPLFMVVYPYTIREVMKFSAQEFGFLETSFTLGALLGNFLIASYLSKKNMWK 287 Query: 313 RRGLMAYGGAFISGVSLLLLPFV--PS-AAGAVILFSL-------EGFGLMVFMLIWEIS 362 + F+ + ++ L F+ P + ++LFSL GF + + + + Sbjct: 288 AMKI----SIFLFELPIVFLGFLVMPDFSLNRLVLFSLFFITFLIMGFLNPIINVPLDTA 343 Query: 363 MQELVPQEAFGRVASLDLLGSFALLPVGYILVGWLAD 399 Q++ P R S +L + + P+G +L G+L D Sbjct: 344 FQKMTPSHIRARAFSFLILVANGVTPLGSVLYGYLVD 380 >FN1168 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 302 Score = 68.6 bits (166), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 49/271 (18%), Positives = 114/271 (42%), Gaps = 14/271 (5%) Query: 23 AKSRAFPFLWLGHLISFLGSSVTMVILPVLVYSLTGSTTTMGVVMAVYMLPNILMLPVSG 82 +K L LG ++S G+++ +++LP+ + ++T + T G+ A LP I++ P+ G Sbjct: 3 SKESNIKLLLLGRVVSLFGNTIYLIVLPLYILNITQNLKTTGIFFASINLPTIIISPLIG 62 Query: 83 HIVDRYDRVGIMMLADIFRALIMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQPAYAA 142 +++++++ I+++ D F ++ F+ A Sbjct: 63 TLIEKFNKKNIILICD-FLTSMLYFIPFLYFKNSNSVIFLLIVSLILNIISKFFEIASKV 121 Query: 143 VRATVFTPDIRVTANSVTQITTQTVRLIGPALGGLLITHLSAGIGFGLDAFTYVFSFICL 202 + + + TP+ N + V + GP +G T+L A T+ F+F+ + Sbjct: 122 LFSEINTPETLEKYNGLQSFLENAVMIFGPVVG----TYLFA---------TFTFNFVLI 168 Query: 203 IYLRRALIARVRTDASGTAGSMAAPSPSADWKQDFMEGLAVLRSHPWLWITILAFSFINI 262 I ++ ++ + + + +DF EG++ ++S ++ + F+N Sbjct: 169 IISLAYFLSFLQELFIKYEKNTNLSKEKSSFFKDFKEGISYIKSKKIIFNFFILAMFLNF 228 Query: 263 CYGGITSILVPWLFKVQHGWDPYLYGLAVTC 293 I+ P + ++ L+G +V C Sbjct: 229 FIANNDEIINPGILIKKYQISEKLFGFSVAC 259 >MA1161 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 443 Score = 66.6 bits (161), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 51/213 (23%), Positives = 90/213 (42%), Gaps = 8/213 (3%) Query: 27 AFPFLWLGHLISFLGSSVTMVILPVLVYSLTGSTTTMGVVMAVYMLPNILMLPVSGHIVD 86 F F+W G IS +GS +T L + VY TG+ ++ ++ LP L+ P G + D Sbjct: 11 KFLFIWFGQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILAD 70 Query: 87 RYDRVGIMMLADIFRALIMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQPAYAAVRAT 146 R+DR +M+L D+ L + +PAY A+ Sbjct: 71 RHDRRLMMILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITD 130 Query: 147 VFTPDIRVTANSVTQITTQTVRLIGPALGGLLITHLSAGIGFGLDAFTYVFSFICLIYLR 206 + + A+ + Q+ + LI P L G+++T + F +D T++ + +I++R Sbjct: 131 LLPENQYAKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIR 190 Query: 207 RALIARVRTDASGTAGSMAAPSPSADWKQDFME 239 + L S T + + AD K+ E Sbjct: 191 KIL--------SRTQITKQEQNNMADLKEGIQE 215 >NMA1161 [P] COG1629 Outer membrane receptor proteins, mostly Fe transport Length = 443 Score = 66.6 bits (161), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 51/213 (23%), Positives = 90/213 (42%), Gaps = 8/213 (3%) Query: 27 AFPFLWLGHLISFLGSSVTMVILPVLVYSLTGSTTTMGVVMAVYMLPNILMLPVSGHIVD 86 F F+W G IS +GS +T L + VY TG+ ++ ++ LP L+ P G + D Sbjct: 11 KFLFIWFGQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILAD 70 Query: 87 RYDRVGIMMLADIFRALIMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFQPAYAAVRAT 146 R+DR +M+L D+ L + +PAY A+ Sbjct: 71 RHDRRLMMILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITD 130 Query: 147 VFTPDIRVTANSVTQITTQTVRLIGPALGGLLITHLSAGIGFGLDAFTYVFSFICLIYLR 206 + + A+ + Q+ + LI P L G+++T + F +D T++ + +I++R Sbjct: 131 LLPENQYAKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIR 190 Query: 207 RALIARVRTDASGTAGSMAAPSPSADWKQDFME 239 + L S T + + AD K+ E Sbjct: 191 KIL--------SRTQITKQEQNNMADLKEGIQE 215 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.142 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,713,668 Number of extensions: 1035767 Number of successful extensions: 4770 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 4765 Number of HSP's successfully gapped: 12 Length of query: 431 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 322 Effective length of database: 25,807,025 Effective search space: 8309862050 Effective search space used: 8309862050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)