BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0055.0007.p
         (159 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

STM0844 [O] COG1180 Pyruvate-formate lyase-activating enzyme           70   2e-12

>STM0844 [O] COG1180 Pyruvate-formate lyase-activating enzyme
          Length = 299

 Score = 69.7 bits (169), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 12/158 (7%), Positives = 28/158 (17%), Gaps = 8/158 (5%)

Query: 1   MFIQIISVGKLKEKYLTLGIAEYAKRLTPYLKFQMIEVADEKAPDNLSEAEVVQVKVREG 60
           M   I       +      +             Q  E    +A       +         
Sbjct: 1   MIFNIQRYSTH-DGPGIRTVVFLKGCSLGCRWCQNPES---RARAQDLLYDARL---CLE 53

Query: 61  ERILAHIKSEAHVIALAIDGKLWSSEELAAELDRLGTYGTSHVVFVIGGSHGLSDDVMRR 120
              L    +   +        +   +   A    L     +     + G     D++M  
Sbjct: 54  GCDLCAQAAPDVIERALNGLLIHREKLTDAHFSILAHCCPT-QALTVCGEIKSVDEIMAT 112

Query: 121 AQQRMSFGRMTLPHQLMRLVLTEQIYRAVKINRGEPYH 158
             +   F   +     +                   + 
Sbjct: 113 VLRDKPFYDRSGGGLTLSGGEPFMQPELAAELFKASHD 150


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.320    0.136    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 11,343,417
Number of extensions: 419687
Number of successful extensions: 827
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 828
Number of HSP's successfully gapped: 1
Length of query: 159
Length of database: 38,378,431
Length adjustment: 98
Effective length of query: 61
Effective length of database: 27,075,699
Effective search space: 1651617639
Effective search space used: 1651617639
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)