BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0055.0006.p (265 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all2891 [R] COG0300 Short-chain dehydrogenases of various substr... 201 1e-51 PA0658 [R] COG0300 Short-chain dehydrogenases of various substra... 179 6e-45 AGl1280 [R] COG0300 Short-chain dehydrogenases of various substr... 177 1e-44 all3753 [R] COG0300 Short-chain dehydrogenases of various substr... 172 6e-43 Rv1544 [R] COG0300 Short-chain dehydrogenases of various substra... 167 2e-41 >all2891 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 258 Score = 201 bits (510), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 108/245 (44%), Positives = 155/245 (63%), Gaps = 3/245 (1%) Query: 11 ALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDLSKA 70 ALITGAS GIG+ +A+ELAAR +++L ARS+ KL LA E+ + H +Q + DL++ Sbjct: 4 ALITGASGGIGKAFAQELAARQTNLVLVARSQHKLHQLAQELQQQHKIQVDVIAKDLTEI 63 Query: 71 EAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHLFLP 130 +A + G+T+D LINNAGFG G F + R+ + I LN ALV+LTH FLP Sbjct: 64 DAVADVFDITKSKGLTIDCLINNAGFGDYGDFAQSDRTRQIKIIQLNVLALVDLTHRFLP 123 Query: 131 GMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALCPGA 190 M + G ++NVAS+A F P YL+VY A+KAF++SFSEALWAE R+ GVRVL CPG Sbjct: 124 LMRQNRSGSIINVASIAGFQPIPYLSVYAASKAFIVSFSEALWAENRQYGVRVLVTCPGP 183 Query: 191 TDTGFF-DAVGSRDMVE-NNTLSTPEKVVQAGFRGVDR-NSGYIIDGRINYMTTLICRIL 247 T+T FF +A + +VE N + + E+VV + ++ II + + + R++ Sbjct: 184 TETDFFAEANFPQALVETTNKVMSSEEVVLKSLKALENWEPTVIISDTSTQLRSNVARLV 243 Query: 248 PRRSL 252 PR++L Sbjct: 244 PRKTL 248 >PA0658 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 266 Score = 179 bits (453), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 104/243 (42%), Positives = 141/243 (58%), Gaps = 2/243 (0%) Query: 9 KVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDLS 68 + ALITGASSGIG A+ LA RG +IL AR +D LE++A E+ + GV+ DLS Sbjct: 3 RYALITGASSGIGLALAEALARRGRALILVARQRDALESIAYELAQRFGVEVLFRTCDLS 62 Query: 69 KAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHLF 128 + L + + +D+L+NNAG GT+G F D REQE I LN AL L H Sbjct: 63 EPLQLSGLLHELEQGERQIDLLVNNAGIGTSGPFIDQEWAREQELIELNILALARLCHSL 122 Query: 129 LPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALCP 188 M + G ++N+AS+AAF P A+++ Y A+KA+VL FSE L E +ERGV+V LCP Sbjct: 123 GARMAAQGGGQILNIASVAAFQPGAWMSNYYASKAYVLHFSEGLREELKERGVKVSVLCP 182 Query: 189 GATDTGFFDAVGSR--DMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICRI 246 G T T FF G R + + + TPE+V RG+D+N I+ G N + L R+ Sbjct: 183 GPTRTAFFREAGMRLGRLDRSKLMMTPEEVALLALRGLDKNRALIVPGWRNRLLALSPRL 242 Query: 247 LPR 249 PR Sbjct: 243 APR 245 >AGl1280 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 134 Score = 177 bits (450), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 0/244 (0%), Positives = 0/244 (0%), Gaps = 3/244 (1%) Query: 8 GKVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDL 67 + A+ + + + + + + + Sbjct: 36 AVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSGRIQTLRV 95 Query: 68 SKAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHL 127 Sbjct: 96 155 Query: 128 FLPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALC 187 Sbjct: 156 215 Query: 188 PGATDTGFFDAVGSR--DMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICR 245 Sbjct: 216 - 274 Query: 246 ILPR 249 Sbjct: 275 278 >all3753 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 263 Score = 172 bits (435), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 98/252 (38%), Positives = 138/252 (54%), Gaps = 4/252 (1%) Query: 1 MNSYHYLGKVALITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQA 60 MN+ H + ALITGA+SGIG A +++L R+K+KLE +A E G+ Sbjct: 1 MNTTH--KQTALITGAASGIGYELVCIFAENDYNLVLVDRAKEKLEEIAIEFQDKFGISV 58 Query: 61 YALPIDLSKAEAPRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAA 120 + DLSK +P + + + + + VD+L+NNAGFGT G F D N E E + +N Sbjct: 59 KPIVRDLSKTTSPEEIFQEVEQANIKVDVLVNNAGFGTYGLFNDTNLTDELEMLQVNVVC 118 Query: 121 LVELTHLFLPGMLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERG 180 LT LFL M+ + +G ++NV+S AAF P +AVY ATKA+VLSFSEAL E G Sbjct: 119 TTHLTKLFLKNMVQQGEGKILNVSSAAAFQPGPLMAVYFATKAYVLSFSEALANELDGTG 178 Query: 181 VRVLALCPGATDTGFFDAVGSRD--MVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINY 238 V V LCPG T + F G D +V+ + V G+RG+ + +I G IN Sbjct: 179 VTVTVLCPGTTQSAFHQRTGMADSKLVKGKRMMDASTVADIGYRGLMQGKTIVIPGLINK 238 Query: 239 MTTLICRILPRR 250 + R +PR+ Sbjct: 239 IMAKSIRFIPRK 250 >Rv1544 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 267 Score = 167 bits (422), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 97/241 (40%), Positives = 138/241 (57%), Gaps = 2/241 (0%) Query: 12 LITGASSGIGEVYAKELAARGCHVILTARSKDKLEALASEITRTHGVQAYALPIDLSKAE 71 +ITGASSGIG A+ LA RG ++L AR +++L+ LA ++ + H V LP+DL+ + Sbjct: 13 VITGASSGIGVELARGLAGRGFPLMLVARRRERLDELADQLRQEHCVGVEVLPLDLADTQ 72 Query: 72 APRLLAKSISELGVTVDILINNAGFGTTGRFEDINPEREQEQIMLNTAALVELTHLFLPG 131 A LA + + L N+AGFGT+GRF ++ RE E+++LN AL+ELTH LPG Sbjct: 73 ARAQLADRLRSDAIAG--LCNSAGFGTSGRFWELPFARESEEVVLNALALMELTHAALPG 130 Query: 132 MLGRKDGVVVNVASMAAFAPCAYLAVYGATKAFVLSFSEALWAETRERGVRVLALCPGAT 191 M+ R G V+N+AS+A F P Y+AVY ATKAFVL+FSEA+ E GV V ALCPG Sbjct: 131 MVKRGAGAVLNIASIAGFQPIPYMAVYSATKAFVLTFSEAVQEELHGTGVSVTALCPGPV 190 Query: 192 DTGFFDAVGSRDMVENNTLSTPEKVVQAGFRGVDRNSGYIIDGRINYMTTLICRILPRRS 251 T + + + +P V +A G+ ++ G + + R PR Sbjct: 191 PTEWAEIASAERFSIPLAQVSPHDVAEAAIAGMLSGKRTVVPGIVPKFVSTSGRFAPRSL 250 Query: 252 L 252 L Sbjct: 251 L 251 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,663,881 Number of extensions: 732065 Number of successful extensions: 2739 Number of sequences better than 1.0e-05: 326 Number of HSP's gapped: 2389 Number of HSP's successfully gapped: 326 Length of query: 265 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 161 Effective length of database: 26,383,695 Effective search space: 4247774895 Effective search space used: 4247774895 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)