BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0004.p (346 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ycgT [O] COG0492 Thioredoxin reductase 379 e-105 BH3408 [O] COG0492 Thioredoxin reductase 308 1e-83 BS_yumC [O] COG0492 Thioredoxin reductase 288 8e-78 lin2489 [O] COG0492 Thioredoxin reductase 280 3e-75 lin2075 [O] COG0492 Thioredoxin reductase 278 1e-74 >BS_ycgT [O] COG0492 Thioredoxin reductase Length = 346 Score = 379 bits (973), Expect = e-105, Method: Compositional matrix adjust. Identities = 17/337 (5%), Positives = 46/337 (13%), Gaps = 2/337 (0%) Query: 1 MSKELELYDVTIIGGGPAGMYTAFYSGMRDLKTKLIEAKEELGGRM-LIYPEKMIWDVGG 59 M + + G T ++ G + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 VTPILCRKLIDQLAEQARTFDPTIVLGQQIIGQERQEDGTYILTSATGERHWTRTIILTI 119 + + + LA+ I + + L Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 120 GYGILQMAKLEIEGADRYEVTNLHYTVQELEPFRGKRVLISGGGDSAVDWANELEPIAAS 179 + ++ Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 180 VTIVHRREQFGGHEKNIARMKASSVQVRVPHTVSQLHSSDGEKIDQVTISHIESGESEQL 239 T +K+ + S E + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFE-VGLAEVSQYEGVELLAYSCLGFG 239 Query: 240 EVDAVIVNHGLKSDFGPLKDWGLDMGEWWANVSSRLETNLPGVFAAGDFVDYSSKVRLIA 299 + +N + + D + Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 300 GTFTDAVLALNSAKLYMDPDAPKVAYVSSHNERFKEK 336 +L + +S E Sbjct: 300 PFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEA 336 >BH3408 [O] COG0492 Thioredoxin reductase Length = 346 Score = 308 bits (788), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 19/321 (5%), Positives = 62/321 (19%), Gaps = 3/321 (0%) Query: 1 MSKELELYDVTIIGGGPAGMYTAFYSGMRDLKTKLIEAKEELGGRML-IYPEKMIWDVGG 59 M + + G T ++ G ++ + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 VTPILCRKLIDQLAEQARTFDPTIVLGQQIIGQERQEDGTYILTSATGERHWTRTIILTI 119 + + + LA+ I + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDR-KNIREALHDSLK 119 Query: 120 GYGILQMAKLEIEGADRYEVTNLHYTVQELEPFRGKRVLISGGGDSAVDWANELEPIAAS 179 + ++ R + +L + + A ++ I S Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 180 VTIVHRREQFGGHEKNIARMKASSVQVRVPHTVSQLHSSDGEKIDQVTISHIESGESEQL 239 ++ + ++Q ++ Q + + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSF-EVGLAEVSQYEGVELLAYSCLGF 238 Query: 240 EVDAVIVNHGLKSDFGPLKDWGLDMGEWWANVSSRLETNLPGVFAAGDFVDYSSKVRLIA 299 +G K + + + G + + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR 298 Query: 300 GTFTDAVLALNSAKLYMDPDA 320 F + L + + + Sbjct: 299 QPFVASTLLGATTMDQLKTNI 319 >BS_yumC [O] COG0492 Thioredoxin reductase Length = 346 Score = 288 bits (738), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 17/321 (5%), Positives = 52/321 (16%), Gaps = 4/321 (1%) Query: 1 MSKELELYDVTIIGGGPAGMYTAFYSGMRDLKTKLIEAKEELGGRM-LIYPEKMIWDVGG 59 M + + G T ++ G + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 VTPILCRKLIDQLAEQARTFDPTIVLGQQIIGQERQEDGTYILTSATGERHWTRTIILTI 119 + + + LA+ I + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDS-LK 119 Query: 120 GYGILQMAKLEIEGADRYEVTNLHYTVQELEPFRGKRVLISGGGDSAVDWANELEPIAAS 179 + ++ R + +L + + A ++ I S Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 180 VTIVHRREQFGGHEKNIARMKASSVQVRVPHTVSQLHSSDGEKIDQVTISHIESGESEQL 239 ++ + ++Q S Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGL--AEVSQYEGVELLAYSCLG 237 Query: 240 EVDAVIVNHGLKSDFGPLKDWGLDMGEWWANVSSRLETNLPGVFAAGDFVDYSSKVRLIA 299 G + + + + + + Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 Query: 300 GTFTDAVLALNSAKLYMDPDA 320 A L + + Sbjct: 298 RQPFVASTLLGATTMDQLKTN 318 >lin2489 [O] COG0492 Thioredoxin reductase Length = 346 Score = 280 bits (715), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 19/319 (5%), Positives = 60/319 (18%), Gaps = 3/319 (0%) Query: 1 MSKELELYDVTIIGGGPAGMYTAFYSGMRDLKTKLIEAKEELGGRM-LIYPEKMIWDVGG 59 M + + G T ++ G + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 VTPILCRKLIDQLAEQARTFDPTIVLGQQIIGQERQEDGTYILTSATGERHWTRTIILTI 119 + + + LA+ I + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDR-KNIREALHDSLK 119 Query: 120 GYGILQMAKLEIEGADRYEVTNLHYTVQELEPFRGKRVLISGGGDSAVDWANELEPIAAS 179 + ++ R + +L + + A ++ I S Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 180 VTIVHRREQFGGHEKNIARMKASSVQVRVPHTVSQLHSSDGEKIDQVTISHIESGESEQL 239 ++ + ++Q ++ Q + + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSF-EVGLAEVSQYEGVELLAYSCLGF 238 Query: 240 EVDAVIVNHGLKSDFGPLKDWGLDMGEWWANVSSRLETNLPGVFAAGDFVDYSSKVRLIA 299 +G K + + + G + + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR 298 Query: 300 GTFTDAVLALNSAKLYMDP 318 F + L + + Sbjct: 299 QPFVASTLLGATTMDQLKT 317 >lin2075 [O] COG0492 Thioredoxin reductase Length = 346 Score = 278 bits (710), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 23/329 (6%), Positives = 70/329 (21%), Gaps = 3/329 (0%) Query: 6 ELYDVTIIGGGPAGMYTAFYSGMRDLKTKLIEAKEELGGRM-LIYPEKMIWDVGGVTPIL 64 + + G+ T + + + + LI +M Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 65 CRKLIDQLAEQARTFDPTIVLGQQIIGQERQEDGTYILTSATGERHWTRTIILTIGYGIL 124 + +++ ++ G R D A ++ + ++ Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 125 QMAKLEIEGADRYEVTNLHYTVQELEPFRGKRVLISGGGDSAVDWANELEPIAASVTIVH 184 L + + V + D+ ++ + V+ Sbjct: 124 DYLDLYQVHWPQRPTN-CFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 185 RREQFGGHEKNIARMKASSVQVRVPHTVSQLHSSDGEKIDQVTISHIESGESEQLEVDAV 244 V ++ P+++ G + +E L + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGL-AEVSQYEGVELLAYSCLGFGTL 241 Query: 245 IVNHGLKSDFGPLKDWGLDMGEWWANVSSRLETNLPGVFAAGDFVDYSSKVRLIAGTFTD 304 + + ++ ++ ++ A +D + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPF 301 Query: 305 AVLALNSAKLYMDPDAPKVAYVSSHNERF 333 L A + +E Sbjct: 302 VASTLLGATTMDQLKTNIESLHLELSEDV 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,155,036 Number of extensions: 1076757 Number of successful extensions: 4607 Number of sequences better than 1.0e-05: 60 Number of HSP's gapped: 4516 Number of HSP's successfully gapped: 60 Length of query: 346 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 239 Effective length of database: 26,037,693 Effective search space: 6223008627 Effective search space used: 6223008627 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)