BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0054.0002.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2072 [P] COG0609 ABC-type Fe3+-siderophore transport system, ... 222 5e-58 BH1083 [P] COG0609 ABC-type Fe3+-siderophore transport system, p... 185 7e-47 BS_fhuB [P] COG0609 ABC-type Fe3+-siderophore transport system, ... 179 3e-45 CAC0789 [P] COG0609 ABC-type Fe3+-siderophore transport system, ... 149 5e-36 SA0603 [P] COG0609 ABC-type Fe3+-siderophore transport system, p... 134 2e-31 >lin2072 [P] COG0609 ABC-type Fe3+-siderophore transport system, permease component Length = 341 Score = 222 bits (565), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 115/270 (42%), Positives = 153/270 (56%) Query: 1 VLGGVIIGASFAVAGAIMQGMTRNPLADSGLMGINSXXXXXXXXXXXXMPNLPFMSLILY 60 V+ + IGA+FAVAGAIMQG+TRNPLADSGL+G+N+ MP L + SLI++ Sbjct: 72 VIADMAIGAAFAVAGAIMQGITRNPLADSGLLGLNAGSTFMVAVCFAFMPWLSYNSLIIF 131 Query: 61 SFXXXXXXXXIVYGVGSLAKGGLTPXXXXXXXXXXXXXXXXXXXXXXXHYKIGQDLALWY 120 SF +V+GV S+A ++P ++++ QDLA WY Sbjct: 132 SFVGAAIGAFLVFGVSSIAGSAMSPTRLVLAGAAVSSLLTALSEGLAIYFQLSQDLAFWY 191 Query: 121 AGGKAGTKWFQLKVMFPWVFAAILGDIVLSRSITMLSLGDEVARGLGQRTXXXXXXXXXX 180 AGG AG KW QL ++PW+ A++ I+LS+SIT+LSLGD++A GLG++T Sbjct: 192 AGGVAGVKWSQLAAIWPWLLGALIAAILLSKSITILSLGDDIAVGLGEKTTFVKIASMVV 251 Query: 181 XXXXXXXXXXXXXXXXFIGLIIPHLTRYLVGVDYRWIFPCSXXXXXXXXXXXXXXXXXXI 240 FIGLI+PHL R+LVGVDYR+I PCS Sbjct: 252 VLILAGLAVSVVGPVGFIGLIVPHLVRFLVGVDYRFIIPCSAVVGAFLTLAADIVARTIN 311 Query: 241 PQHETPLGAVLALIGVPFFLYLARKERREL 270 P +ETP+ + ALIGVPFFLY+ARKERR L Sbjct: 312 PPYETPISVIFALIGVPFFLYVARKERRNL 341 >BH1083 [P] COG0609 ABC-type Fe3+-siderophore transport system, permease component Length = 338 Score = 185 bits (469), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 99/269 (36%), Positives = 137/269 (50%) Query: 2 LGGVIIGASFAVAGAIMQGMTRNPLADSGLMGINSXXXXXXXXXXXXMPNLPFMSLILYS 61 + ++GA A++GAIMQGMTRNPLA ++G+ +P + F+ + S Sbjct: 70 VAAALVGACLAISGAIMQGMTRNPLASPSILGLTDGSTLAIAIAFAFIPGISFLQTMFMS 129 Query: 62 FXXXXXXXXIVYGVGSLAKGGLTPXXXXXXXXXXXXXXXXXXXXXXXHYKIGQDLALWYA 121 F +V+ + LAKG L+P H+++ Q ++ WYA Sbjct: 130 FAGAGVAAALVFSIVFLAKGQLSPVKLALAGVAIGALLDSLATGIAIHFQVAQHVSFWYA 189 Query: 122 GGKAGTKWFQLKVMFPWVFAAILGDIVLSRSITMLSLGDEVARGLGQRTXXXXXXXXXXX 181 GG AGT W Q++ + P + + ++RSIT+LSLGDEVA GLGQRT Sbjct: 190 GGVAGTNWTQIQFLVPVFLLGLFLSLFIARSITVLSLGDEVAVGLGQRTFFVRIVAILAV 249 Query: 182 XXXXXXXXXXXXXXXFIGLIIPHLTRYLVGVDYRWIFPCSXXXXXXXXXXXXXXXXXXIP 241 F+GLIIPH+TR+LVGVDYR + PCS P Sbjct: 250 LLLTGAAVSVAGAVGFVGLIIPHVTRFLVGVDYRVVLPCSAVFGALLLVLADIGSRMVNP 309 Query: 242 QHETPLGAVLALIGVPFFLYLARKERREL 270 ETP+GA+ AL+GVPFFLYLAR++RR L Sbjct: 310 PFETPIGAITALVGVPFFLYLARRDRRGL 338 >BS_fhuB [P] COG0609 ABC-type Fe3+-siderophore transport system, permease component Length = 384 Score = 179 bits (455), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 98/257 (38%), Positives = 129/257 (50%) Query: 14 AGAIMQGMTRNPLADSGLMGINSXXXXXXXXXXXXMPNLPFMSLILYSFXXXXXXXXIVY 73 +GAIMQGMTRNPLA+ +MG+ S P L M L+L+SF V Sbjct: 128 SGAIMQGMTRNPLAEPSIMGVTSGSAFAVSIAFAFFPGLSAMGLVLWSFAGAGLGASTVM 187 Query: 74 GVGSLAKGGLTPXXXXXXXXXXXXXXXXXXXXXXXHYKIGQDLALWYAGGKAGTKWFQLK 133 G+G ++GGLTP + + QD++ WYAGG AG KW ++ Sbjct: 188 GIGMFSRGGLTPVKLALAGTAVTYFFTGISTAIAIRFDVAQDISFWYAGGVAGVKWSGVQ 247 Query: 134 VMFPWVFAAILGDIVLSRSITMLSLGDEVARGLGQRTXXXXXXXXXXXXXXXXXXXXXXX 193 ++ + ++RS+T+LSLGD++A+GLGQ T Sbjct: 248 LLLIAGAVGLTLAFFIARSVTVLSLGDDLAKGLGQYTSAVKLVGMLIVVILTGAAVSIAG 307 Query: 194 XXXFIGLIIPHLTRYLVGVDYRWIFPCSXXXXXXXXXXXXXXXXXXIPQHETPLGAVLAL 253 FIGLIIPH+TR+LVGVDYRWI PCS ETP+GA+ +L Sbjct: 308 TIAFIGLIIPHITRFLVGVDYRWIIPCSAVLGAVLLVFADIAARLVNAPFETPVGALTSL 367 Query: 254 IGVPFFLYLARKERREL 270 IGVPFF YLAR+ERR L Sbjct: 368 IGVPFFFYLARRERRGL 384 >CAC0789 [P] COG0609 ABC-type Fe3+-siderophore transport system, permease component Length = 334 Score = 149 bits (376), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 74/264 (28%), Positives = 132/264 (50%) Query: 2 LGGVIIGASFAVAGAIMQGMTRNPLADSGLMGINSXXXXXXXXXXXXMPNLPFMSLILYS 61 + V +GA+ AV+GAIMQG+++NPLAD GL+G+ + P+ ++++++ Sbjct: 66 IAAVFVGAALAVSGAIMQGVSKNPLADPGLLGLTAGANAALTITITIAPSANYLTILISC 125 Query: 62 FXXXXXXXXIVYGVGSLAKGGLTPXXXXXXXXXXXXXXXXXXXXXXXHYKIGQDLALWYA 121 F +V+G+ +GG +P ++K+ +D+++W + Sbjct: 126 FLGAAAAVVLVFGISFSKRGGSSPLRIVLAGSAVSTFLFAISQGMQIYFKLSKDVSMWTS 185 Query: 122 GGKAGTKWFQLKVMFPWVFAAILGDIVLSRSITMLSLGDEVARGLGQRTXXXXXXXXXXX 181 GG GT W +L V+ P++ + +LSR +T+LSL +EVA GLGQ+T Sbjct: 186 GGLIGTSWDELAVIIPFITLGLATAFILSRQLTVLSLSEEVAVGLGQKTTPIKAILFVVV 245 Query: 182 XXXXXXXXXXXXXXXFIGLIIPHLTRYLVGVDYRWIFPCSXXXXXXXXXXXXXXXXXXIP 241 F+GL++PH+ +++G DYR++ P S Sbjct: 246 TILAGASVALASNLVFLGLMVPHIAYFIIGKDYRFVIPISTVLGAALMVFSDILGRLVAS 305 Query: 242 QHETPLGAVLALIGVPFFLYLARK 265 +ETP+ A++++IG+PFFL++ K Sbjct: 306 PYETPVAAIISIIGLPFFLFIVHK 329 >SA0603 [P] COG0609 ABC-type Fe3+-siderophore transport system, permease component Length = 334 Score = 134 bits (336), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 78/269 (28%), Positives = 121/269 (44%), Gaps = 3/269 (1%) Query: 2 LGGVIIGASFAVAGAIMQGMTRNPLADSGLMGINSXXXXXXXXXXXXMPNLPFMSLILYS 61 + VI+G + AV+GAI+QG+TRN LAD L+G+NS +PN F+ L+ Sbjct: 69 IAAVIVGMALAVSGAIIQGVTRNGLADPALIGLNSGASFALALTYAVLPNTSFLILMFAG 128 Query: 62 FXXXXXXXXIVYGVGSLAKGGLTPXXXXXXXXXXXXXXXXXXXXXXXHYKIGQDLALWYA 121 F IV +G + G P +++ Q + W A Sbjct: 129 FLGAILGGAIVLMIGRSRRDGFNPMRIILAGAAVSAMLTALSQGIALAFRLNQTVTFWTA 188 Query: 122 GGKAGTKWFQLKVMFPWVFAAILGDIVLSRSITMLSLGDEVARGLGQRTXXXXXXXXXXX 181 GG +GT W LK P + A+ + +S+ +T+L+LG+ +A+GLGQ Sbjct: 189 GGVSGTTWSHLKWAIPLIGIALFIILTISKQLTILNLGESLAKGLGQNVTMIRGICLIIA 248 Query: 182 XXXXXXXXXXXXXXXFIGLIIPHLTRYLVGVDYRWIFPCSXXXXXXXXXXXXXXXXXXIP 241 F+GL++PH+ R+L+G DY I P + Sbjct: 249 MILAGIAVAIAGQVAFVGLMVPHIARFLIGTDYAKILPLTALLGGILVLVADVIARYL-- 306 Query: 242 QHETPLGAVLALIGVPFFLYLARKERREL 270 E P+GA+++ IGVP+FLYL +K R + Sbjct: 307 -GEAPVGAIISFIGVPYFLYLVKKGGRSI 334 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.146 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,773,280 Number of extensions: 339104 Number of successful extensions: 1248 Number of sequences better than 1.0e-05: 112 Number of HSP's gapped: 1051 Number of HSP's successfully gapped: 123 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)