BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0053.0005.p (488 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1894 [G] COG0366 Glycosidases 376 e-104 AGpT251 [G] COG0366 Glycosidases 304 2e-82 ZycjMm [G] COG0366 Glycosidases 161 2e-39 ycjM [G] COG0366 Glycosidases 157 4e-38 ZycjMm [G] COG0366 Glycosidases 157 4e-38 >SP1894 [G] COG0366 Glycosidases Length = 480 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 195/469 (41%), Positives = 285/469 (60%), Gaps = 10/469 (2%) Query: 2 VKNQVQLITYPDSLGGDLKSLHHALDVYFPDVFKGGVHILPPFPSSGDRGFAPLTYLEIE 61 ++N+ LITY DSLG +LK L+ L+ +F D GGVH+LP FPS+ DRGFAP+ Y E++ Sbjct: 3 IQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAI-GGVHLLPFFPSTVDRGFAPVDYDEVD 61 Query: 62 PAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADG 121 AFGDW D++ +GE++ ++ D M+NHISRQS Y++D + S + LFL DK W + Sbjct: 62 SAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKHEASEFKALFLNWDKFWPEN 121 Query: 122 QPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFIQ 181 +P Q+D+D ++ R+ + + + G +W TFG+ EQIDLD+ E + + Sbjct: 122 RPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGE----EQIDLDVTKEVTMEFIRK 177 Query: 182 FFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVHS 241 N ++RLDA Y +KKL T+ FFVEP+I+ LD ++++A ELLPE+H Sbjct: 178 TIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLDKVRDIAAEYGTELLPEIHE 237 Query: 242 HYSIQYKLAEHGCWIYDFILPYRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPVK 301 HYSIQ+K+A+H ++YDF LP L TL + + L ++L+ P KQFT LD HDG+ V Sbjct: 238 HYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKWLKMSPMKQFTTLDTHDGIGV- 296 Query: 302 PDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDAY 361 D+ D++ ++ + + GAN+ S + D++QI +YYS L DD Y Sbjct: 297 VDVKDILTDEEIDYASNELYKVGANVKRKYSSAEY--NNLDIYQINSTYYSALGDDDVKY 354 Query: 362 LAARAIQFFAPGIPQVYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVVQ 421 AR IQ FAPGIPQ+YYVGLLAG+NDL+ + ET EGR INR Y+ EI + +++ VV+ Sbjct: 355 FLARLIQAFAPGIPQIYYVGLLAGKNDLKLLEETKEGRNINRHYYSNEEIAKEVQRPVVK 414 Query: 422 RLLKLIRFRNEYEAFN--GDFTVEEAAADEIRLSWKKGEFRCTLYINLD 468 LL L FRN EAF+ G +E A I + + + T + +D Sbjct: 415 ALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQNKDKSVTAVVEID 463 >AGpT251 [G] COG0366 Glycosidases Length = 35 Score = 304 bits (779), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 0/458 (0%), Positives = 0/458 (0%), Gaps = 19/458 (4%) Query: 26 LDVYFPDVFKGGVHILPPF-PSSG-DRGFAPLTYLEIEPAFGDWADIRAIGERHDVLVDL 83 + + L + Sbjct: 5 IGRLSSRAG-CRLKPLTSIVKLTWR 63 Query: 84 MVNHISRQSPYFQDVLQHGRQSRYADLFLTLDKIWADGQPVQADIDKMFLRRSK-PYSTF 142 Sbjct: 64 - 122 Query: 143 TIQDTGEELQVWTTFGKNDPSEQIDLDIRSEKVKQLFIQFFTCFKENNIKIVRLDAVGYV 202 Sbjct: 123 ----- 177 Query: 203 IKKLGTSCFFVEPEIYGFLDWIKELADSLDIELLPEVHSHYSIQYKLAEHGCWIYDFILP 262 Sbjct: 178 - 236 Query: 263 YRILDTLLNKDSRGLREYLRTRPAKQFTMLDCHDGVPV------KPDLDDLIDTKDAQKI 316 Sbjct: 237 296 Query: 317 VDICVERGANLSLILSDEHKAPDGFDVHQIRCSYYSVLNCDDDAYLAARAIQFFAPGIPQ 376 Sbjct: 297 -- 354 Query: 377 VYYVGLLAGENDLERVRETGEGREINRRNYTLPEIGQSLEKEVVQRLLKLIRFRNEYEAF 436 Sbjct: 355 414 Query: 437 NGDFTVEEAAADEIRLSWKKGEFRCTLYINLDTMRSEI 474 Sbjct: 415 452 >ZycjMm [G] COG0366 Glycosidases Length = 568 Score = 161 bits (407), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 22/494 (4%), Positives = 79/494 (15%), Gaps = 61/494 (12%) Query: 3 KNQVQLITYPDSL-GGDLKSL-------HHALDVYFPDVFKGGVHILPPFPSSGDRGFAP 54 + + + F + F F P Sbjct: 46 TQRRKKHWDESDVVLITYADQFHSNDLKPLPTFNQF-----YHQWLQSIFSHVHLLPFYP 100 Query: 55 LTYLEIEPAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTL 114 + + GE D+ +H+ + + L Sbjct: 101 WSSDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAK--SEWFKNYLQQHP 158 Query: 115 DKIWADGQPVQADIDKMFLRRSKPYSTFTIQDTGEELQVWTTFGKNDPSEQIDLDIRSEK 174 + + + T + + ++ Sbjct: 159 GF--------EDFFIAVDPQTDLSAVTRPRALPLLTPFQMRDHS----TRHLWTTFSDDQ 206 Query: 175 VKQLFIQFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDIE 234 + + ++ + L ++ + E + K + Sbjct: 207 IDLNYRSPEVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKE-PGTSCIHLEKTHLIIKLLR 265 Query: 235 LLPEVHSHYSIQYKLAEHG------CWIYDFILPYRILDTLLNKDSRGLREY-----LRT 283 + + + ++ + + + L + L Sbjct: 266 SIIDNVAPGTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCA 325 Query: 284 RPAKQFTMLDCHDGVPVKPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGF-D 342 D I + ++ + + Sbjct: 326 WAQNLTLPSSNTTWFNFLA-SHDGIGLNPLRGLLPESEILEL-----VEALQQEGALVNW 379 Query: 343 VHQIRCSYYSVLNCDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRET 395 + + F + + + Sbjct: 380 KNNPDGTRSPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSR 439 Query: 396 GEGREINRRNYTLPEIGQSLEKEVVQR------LLKLIRFRNEYEAFNGD--FTVEEAAA 447 + + + Y + + + R L+ + Sbjct: 440 NDYAGVEKLGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDN 499 Query: 448 DEIRLSWKKGEFRC 461 + + R Sbjct: 500 NFTIDTINSSVMRI 513 >ycjM [G] COG0366 Glycosidases Length = 568 Score = 157 bits (396), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 137/516 (26%), Positives = 232/516 (44%), Gaps = 64/516 (12%) Query: 3 KNQVQLITYPDSL-GGDLKSL-------HHALDVYFPDVFKGGVHILPPFPSSGDRGFAP 54 ++ V LITY D DLK L H L F VH+LP +P S D GF+ Sbjct: 55 ESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIF-----SHVHLLPFYPWSSDDGFSV 109 Query: 55 LTYLEIEPAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTL 114 + Y ++ G+W DI+ +GE ++ D + NH+S +S +F++ LQ + + D F+ + Sbjct: 110 IDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQ--QHPGFEDFFIAV 167 Query: 115 DKIWADGQPVQADIDKMFLRRSKPYST-FTIQDTGEELQVWTTFGKNDPSEQIDLDIRSE 173 D Q D+ + R+ P T F ++D +WTTF +QIDL+ RS Sbjct: 168 DP--------QTDLSAVTRPRALPLLTPFQMRDHSTR-HLWTTFS----DDQIDLNYRSP 214 Query: 174 KVKQLFIQFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDI 233 +V + C+ + VRLDAVG++ K+ GTSC +E + + + ++ + D++ Sbjct: 215 EVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGTSCIHLE-KTHLIIKLLRSIIDNVAP 273 Query: 234 ELLPEVHSHYSIQYKLAEHGC------WIYDFILPYRILDTLLNKDSRGLREYLR--TRP 285 + ++ + +A G +Y F LP +L + ++ L + + T P Sbjct: 274 GTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLP 333 Query: 286 AKQ---FTMLDCHDGVPVKPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGF- 341 + F L HDG+ + P L L+ + ++V+ + GA L + PDG Sbjct: 334 SSNTTWFNFLASHDGIGLNP-LRGLLPESEILELVEALQQEGA-----LVNWKNNPDGTR 387 Query: 342 DVHQIRCSYYSVLNCDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRE 394 ++I +Y L+ + + ++ A AI PG+P +Y +L ND V + Sbjct: 388 SPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEK 447 Query: 395 TGEGREINRRNYTLPEIGQSLEKEVVQR------LLKLIRFRNEYEAFNGD--FTVEEAA 446 G R INR+ Y EI + L E R L +LI R + F+ D FT++ Sbjct: 448 LGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTIN 507 Query: 447 ADEIRLSWKKGEFRC-TLYINLDTMRSEIEYTDGEG 481 + +R+ + C T N+ + T+ G Sbjct: 508 SSVMRIPRSNADGNCLTGLFNVSKNIQHVNITNLHG 543 Score = 157 bits (396), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 137/516 (26%), Positives = 232/516 (44%), Gaps = 64/516 (12%) Query: 3 KNQVQLITYPDSL-GGDLKSL-------HHALDVYFPDVFKGGVHILPPFPSSGDRGFAP 54 ++ V LITY D DLK L H L F VH+LP +P S D GF+ Sbjct: 55 ESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIF-----SHVHLLPFYPWSSDDGFSV 109 Query: 55 LTYLEIEPAFGDWADIRAIGERHDVLVDLMVNHISRQSPYFQDVLQHGRQSRYADLFLTL 114 + Y ++ G+W DI+ +GE ++ D + NH+S +S +F++ LQ + + D F+ + Sbjct: 110 IDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQ--QHPGFEDFFIAV 167 Query: 115 DKIWADGQPVQADIDKMFLRRSKPYST-FTIQDTGEELQVWTTFGKNDPSEQIDLDIRSE 173 D Q D+ + R+ P T F ++D +WTTF +QIDL+ RS Sbjct: 168 DP--------QTDLSAVTRPRALPLLTPFQMRDHSTR-HLWTTFS----DDQIDLNYRSP 214 Query: 174 KVKQLFIQFFTCFKENNIKIVRLDAVGYVIKKLGTSCFFVEPEIYGFLDWIKELADSLDI 233 +V + C+ + VRLDAVG++ K+ GTSC +E + + + ++ + D++ Sbjct: 215 EVLLAMVDVLLCYLAKGAEYVRLDAVGFMWKEPGTSCIHLE-KTHLIIKLLRSIIDNVAP 273 Query: 234 ELLPEVHSHYSIQYKLAEHGC------WIYDFILPYRILDTLLNKDSRGLREYLR--TRP 285 + ++ + +A G +Y F LP +L + ++ L + + T P Sbjct: 274 GTVIITETNVPHKDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLP 333 Query: 286 AKQ---FTMLDCHDGVPVKPDLDDLIDTKDAQKIVDICVERGANLSLILSDEHKAPDGF- 341 + F L HDG+ + P L L+ + ++V+ + GA L + PDG Sbjct: 334 SSNTTWFNFLASHDGIGLNP-LRGLLPESEILELVEALQQEGA-----LVNWKNNPDGTR 387 Query: 342 DVHQIRCSYYSVLNCDDDA-------YLAARAIQFFAPGIPQVYYVGLLAGENDLERVRE 394 ++I +Y L+ + + ++ A AI PG+P +Y +L ND V + Sbjct: 388 SPYEINVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEK 447 Query: 395 TGEGREINRRNYTLPEIGQSLEKEVVQR------LLKLIRFRNEYEAFNGD--FTVEEAA 446 G R INR+ Y EI + L E R L +LI R + F+ D FT++ Sbjct: 448 LGYNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTIN 507 Query: 447 ADEIRLSWKKGEFRC-TLYINLDTMRSEIEYTDGEG 481 + +R+ + C T N+ + T+ G Sbjct: 508 SSVMRIPRSNADGNCLTGLFNVSKNIQHVNITNLHG 543 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,797,326 Number of extensions: 1738863 Number of successful extensions: 4350 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 4340 Number of HSP's successfully gapped: 15 Length of query: 488 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 378 Effective length of database: 25,691,691 Effective search space: 9711459198 Effective search space used: 9711459198 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)