BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0049.0002.p (318 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2261 [R] COG3481 Predicted HD-superfamily hydrolase 227 2e-59 BH1175 [R] COG3481 Predicted HD-superfamily hydrolase 217 2e-56 BH3191 [R] COG3481 Predicted HD-superfamily hydrolase 132 9e-31 CAC0749 [R] COG3481 Predicted HD-superfamily hydrolase 64 3e-10 >CAC2261 [R] COG3481 Predicted HD-superfamily hydrolase Length = 322 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 123/315 (39%), Positives = 190/315 (60%), Gaps = 6/315 (1%) Query: 4 IKQLSPQDEFVGFYLLRELAIKQTNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFF 63 I +L + GF++++ + ++ + K Y D GD +G I+ K WDAS D++ F Sbjct: 6 ISELEAGNRIDGFFIIK--SAEKRISSNNKKYLDFTFGDKTGDINGKLWDASDEDEDAFV 63 Query: 64 PMALVKIRGIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMAS 123 LVK+RG ++ LQ+KI KIR D DNV + DF+ SAP P D+ TI + Sbjct: 64 DNMLVKVRGTVIEWQNNLQLKIDKIRKTVDSDNVKVADFVPSAPYLPDDMYSTILEYVEK 123 Query: 124 ITDPEIATIVSFCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLN 183 I + +I I+ + +++M++PAAK +HH+ +GL YH ML++GE + + FLN Sbjct: 124 IKNADIKNILYDVLESAGEEIMYWPAAKKNHHSVRSGLLYHTFTMLKVGEKISEIYTFLN 183 Query: 184 PDLMKAGIILHDIAKPEEMI-SQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLES 242 DL+ AG+ILHD+AK EEM+ S+ GIV+DYSV+G+L+GHI + + +A + G D Sbjct: 184 TDLIYAGVILHDMAKLEEMVSSELGIVTDYSVEGQLLGHIILGVEKVRNSAKKVGAD--K 241 Query: 243 EKVLGLQHLVLSHHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWT 302 E + L+H+VLSHH E+GSP +P EA LHH+D +DA + ++ AL+TT E + Sbjct: 242 EISMMLEHMVLSHHYEAEYGSPKKPMFPEAEVLHHLDDMDAAMFDMKKALETTAPG-EMS 300 Query: 303 PFIRGLENKAVYRMK 317 I L+ + +Y+ K Sbjct: 301 DAIWSLDRRRIYKSK 315 >BH1175 [R] COG3481 Predicted HD-superfamily hydrolase Length = 320 Score = 217 bits (553), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 121/301 (40%), Positives = 181/301 (60%), Gaps = 10/301 (3%) Query: 17 YLLRELAIKQ--TNGTPPKDYFDIVLGDSSGQISAKYWDASTTDKETFFPMALVKIRGIA 74 Y L + A K +NG P + ++L D +G+I AK W S D+ TF A+V I G Sbjct: 17 YFLIKSATKAVASNGKP---FLTLILSDHTGEIEAKLWGCSPEDEATFVSGAIVHISGQL 73 Query: 75 HTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKGVMASITDPEIATIVS 134 YR + Q+KI IR D V ++DF+RSAP+ P D++ I + + +P+I + Sbjct: 74 SEYRGRQQLKIGSIRPTTAMDQVKVSDFVRSAPLSPDDMLEQITQYIFEMKNPKIQRMTR 133 Query: 135 FCVGKVEKKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCKQRPFLNPDLMKAGIILH 194 + K + + YPAA T+HH + +GLAYH+V ML + + L P L+ DL+ AGIILH Sbjct: 134 HLLKKHQTAFLEYPAATTNHHEFVSGLAYHVVCMLNVAKSLAALYPTLDTDLLYAGIILH 193 Query: 195 DIAKPEEMISQFGIVSDYSVQGKLIGHISMASNWITEAAIRSGIDLESEKVLGLQHLVLS 254 D+ K +E+ I + Y+++GKL+GHIS+ N I E A GI E E+V+ LQHLVL+ Sbjct: 194 DLGKVKELSGP--IDTTYTIEGKLLGHISILVNEIGETANELGI--EGEEVIILQHLVLA 249 Query: 255 HHNLGEWGSPVQPQTAEAVALHHIDALDAKLQMVEDALDTTPETEEWTPFIRGLENKAVY 314 HH+ GEWGSP P EA LH ID +DAK+ M++ AL+ + E++ I+ ++N++ Y Sbjct: 250 HHSKGEWGSPKPPLIREAEILHMIDNIDAKMNMMDRALERV-QPGEFSERIKAMDNRSFY 308 Query: 315 R 315 + Sbjct: 309 K 309 >BH3191 [R] COG3481 Predicted HD-superfamily hydrolase Length = 315 Score = 132 bits (331), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 92/307 (29%), Positives = 156/307 (50%), Gaps = 12/307 (3%) Query: 1 MTLIKQLSPQDEFVGFYLLRELAIKQ-TNGTPPKDYFDIVLGDSSGQISAKYWDASTTDK 59 MT + + V FY++R+ +K+ +NGT DY ++ L I A+ WDA+ K Sbjct: 1 MTRFQDRQEGERVVDFYMIRDREVKRASNGT---DYLNLTLERKLEYIPARLWDATEQHK 57 Query: 60 ETFFPMALVKIRGIAHTYREKLQVKITKIRLANDGDNVSLTDFIRSAPIRPVDLIHTIKG 119 + A+VK+ G+ YR++ Q+ I +IRLA D D V++ + I + +L H ++ Sbjct: 58 QALIRRAVVKVDGVVTVYRQQKQLNIQRIRLATDEDPVTVAELISKPGLSREELWHELRM 117 Query: 120 VMASITDPEIATIVSFCVGKVE--KKLMHYPAAKTHHHAYFAGLAYHMVRMLEIGEFLCK 177 ++ I D + +V +GK E ++ PA+K HH Y+AGL H+V + + L Sbjct: 118 IIEEIQDETLLKLVKTMLGKKEIRERFTTIPASKATHHPYYAGLLEHVVEVCQSILQLSL 177 Query: 178 QRPFLNPDLMKAGIILHDIAKPEEMISQFGIVSDYSVQGKLIGHISMASNWITEAAIRSG 237 +N D++ A ILHDI K + F +Y+ +G++IGH+ + I + A G Sbjct: 178 IYGTINRDVVIAAAILHDIGKVAALHDPFA--PEYTDEGEMIGHVVLGVELINQTAQELG 235 Query: 238 IDLESEKVLGLQHLVLSHHNLGEWGSPV--QPQTAEAVALHHIDALDAKLQMVEDALDTT 295 I ++ +L ++H +LS E + P+T EA+ L +D L+ +L E L + Sbjct: 236 ISMDEPILLAVKHCILSQREQDEGATSFVYGPKTKEAILLAALDQLNGRLNSFE--LMAS 293 Query: 296 PETEEWT 302 E WT Sbjct: 294 HAEERWT 300 >CAC0749 [R] COG3481 Predicted HD-superfamily hydrolase Length = 300 Score = 63.9 bits (154), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 41/159 (25%), Positives = 79/159 (49%), Gaps = 5/159 (3%) Query: 154 HHAYFAGLAYHMVRMLEIGEFLCKQRPFLNPDLMKAGIILHDIAKPEEMISQFGIVSDYS 213 HH Y GLA H + +++I L ++ ++ LHDI K E Y+ Sbjct: 143 HHNYIGGLAEHTLNVMKITAKLSEKYKCRRMEVAVLAAKLHDIGKVYEYDCNGPF--KYT 200 Query: 214 VQGKLIGHISMASNWITEAAIRSGIDLESEKVLG-LQHLVLSHHNLGEWGSPVQPQTAEA 272 ++G++ GHI + + + A+R ++L E + ++ ++ HH E+GSP + E+ Sbjct: 201 MRGQMEGHIVIGVELV-DRAVRENLNLYPEDFVNRIKGCIVQHHGKLEFGSPREMNMEES 259 Query: 273 VALHHIDALDAKLQMVEDALDTTPETEEWTPFIRGLENK 311 +++ D++DA + + + T E + W+ F R +E K Sbjct: 260 FIINYADSIDATMNKISQLKEKT-EPDTWSEFDRRIERK 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,732,995 Number of extensions: 928357 Number of successful extensions: 1493 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 1486 Number of HSP's successfully gapped: 4 Length of query: 318 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 212 Effective length of database: 26,153,027 Effective search space: 5544441724 Effective search space used: 5544441724 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)