BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0009.p (339 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1726 [R] COG0820 Predicted Fe-S-cluster redox enzyme 259 5e-69 aq_416 [R] COG0820 Predicted Fe-S-cluster redox enzyme 229 5e-60 TM1715 [R] COG0820 Predicted Fe-S-cluster redox enzyme 217 2e-56 PA3806 [R] COG0820 Predicted Fe-S-cluster redox enzyme 209 5e-54 PA1839 [R] COG0820 Predicted Fe-S-cluster redox enzyme 130 4e-30 >CAC1726 [R] COG0820 Predicted Fe-S-cluster redox enzyme Length = 345 Score = 259 bits (661), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 138/329 (41%), Positives = 206/329 (62%), Gaps = 12/329 (3%) Query: 13 QMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEHFVFQTMDEHVKQ 72 ++ W+ ++G K RA + ++ +Y DF +M + T + L ++F + M VK+ Sbjct: 11 ELKQWMDKNGEKTFRAKQFFDWIYNGTF-DFKDMKNLPQSTRERLEKNF-YIGMPSVVKR 68 Query: 73 -ESADG-TIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCASGLLAKSRDL 130 S G T+KFLFR DGN+IE V+M+ YG S+C++TQVGC +GCSFCAS + + RDL Sbjct: 69 LNSKKGDTVKFLFRYNDGNIIECVVMKYDYGNSICISTQVGCRMGCSFCASTIGGRVRDL 128 Query: 131 SSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTIIKDHKGLAIAG 190 +SGEI+ QI+K Q + G+R+S++V+MG GEP DN+ N++ F+ I+ KGL I Sbjct: 129 TSGEILAQILKAQKEI-----GERISNIVLMGSGEPLDNYDNVIKFIRIVNSEKGLNIGQ 183 Query: 191 KGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPIEKLMPAIDYY 250 + IT+ST G+ +I + N+Q+ LAISLHA ++E R +IM I I +++ Y Sbjct: 184 RHITLSTCGIVPRIYDLMKENLQITLAISLHASDDETRKKIMPIANRYSISEIIDCCKKY 243 Query: 251 LEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVDEHSQYQRSER 310 + T RRIT EY L+KDVND +E A +L EL+ G + +VNLIP N V+E S Y++ E Sbjct: 244 SDFTGRRITFEYSLVKDVNDDKESAKKLGELLSG--MLCHVNLIPVNTVNETS-YEKPES 300 Query: 311 ESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 ++ F DTL K + + R E G DI+A Sbjct: 301 SKIKKFCDTLLKYKIESTIRKEMGADINA 329 >aq_416 [R] COG0820 Predicted Fe-S-cluster redox enzyme Length = 348 Score = 229 bits (584), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 122/330 (36%), Positives = 204/330 (61%), Gaps = 8/330 (2%) Query: 10 TLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEHFVFQTMDEH 69 TLE++ E G + RA +++ +Y++ V DF +MT + + +LL EHF F +++ Sbjct: 8 TLEELKKRFTELGLEPYRAKQVFRWVYKKFVTDFEKMTDLGKKHRELLKEHFAFHPLEKL 67 Query: 70 VKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCASGLLAKSRD 129 + E+ D +K+LF+ KDG+++ETVL++++ ++CV++Q+GC +GC+FCA+ L R+ Sbjct: 68 DRVEAPDA-VKYLFKTKDGHILETVLIKERDHYTLCVSSQIGCAVGCTFCATALDGLKRN 126 Query: 130 LSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTIIKDHKGLAIA 189 LS+ EI++Q ++VQ L +++ +VV MG+GEP N+ N+ + I+ +GL ++ Sbjct: 127 LSTAEIIDQYLQVQQDLG----EEKIRNVVFMGMGEPLANYENVRKAVEIMVSPEGLDLS 182 Query: 190 GKGITVSTSGLADKIREFA-DANM-QVNLAISLHAPNNELRTRIMKINRAIPIEKLMPAI 247 + IT+STSG+ +I+ A D M +VNLA+SL+A + + R +M + + +E+LM + Sbjct: 183 KRRITISTSGIVAQIKRMAQDPVMKEVNLAVSLNAVSQKKREELMPLTKTNTLEELMEVL 242 Query: 248 DYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVDEHSQYQR 307 Y RRITLEY+L+K VND A LA+LIG ++ VNLIP+NP D + Y+R Sbjct: 243 KNYPLPKYRRITLEYVLIKGVNDSPNDAERLAKLIGRHKKKFKVNLIPFNP-DPNLPYER 301 Query: 308 SERESVRAFFDTLKKQGVSVSTRLEHGVDI 337 + F L K G+S R GV++ Sbjct: 302 PALTDIMKFQKVLWKYGISNFVRFSKGVEV 331 >TM1715 [R] COG0820 Predicted Fe-S-cluster redox enzyme Length = 343 Score = 217 bits (552), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 119/339 (35%), Positives = 201/339 (59%), Gaps = 14/339 (4%) Query: 4 QSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEHF-- 61 +++ L+ E++ + + G ++ RA +I + ++ ++V +F EMT ++ + LL EHF Sbjct: 2 KNLLDLSYEELVTEITNLGLERYRADQILDWVFDKKVNNFDEMTNLSKKHRALLKEHFSI 61 Query: 62 -VFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCA 120 + +D+ V + DGT KFL+ L+DGN IE+V++ ++ C++TQVGC + C FCA Sbjct: 62 SFLKLLDKKVSR--IDGTTKFLWELEDGNTIESVMLFHPDRITACISTQVGCPVKCIFCA 119 Query: 121 SGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 +G+ R+L++GEIV QI+ ++ +++ +VV MG+GEP N+ N + + I+ Sbjct: 120 TGMSGFVRNLTTGEIVAQILSMEKEEK-----KKIGNVVYMGMGEPLLNYENTIKSIRIL 174 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 K I + IT+ST G+ D+I + A+ + V LA+SLHAP N R +++ +N+ I Sbjct: 175 NHKKMGNIGIRRITISTVGIPDRIIQLAEEGLDVKLALSLHAPTNFKRDQLVPLNKKYSI 234 Query: 241 EKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNPVD 300 E+++ A+ Y KT R+T+EY+L++ +ND A +LAE++ R VNLIP NP Sbjct: 235 EEILNAVKIYQRKTGNRVTIEYVLIRGINDEISDAKKLAEIL--RNMKVFVNLIPVNPTV 292 Query: 301 EHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 E +R RE + F L + G+ R E G DI+A Sbjct: 293 EG--LRRPSRERLLTFKRILLENGIEAEIRREKGTDIEA 329 >PA3806 [R] COG0820 Predicted Fe-S-cluster redox enzyme Length = 379 Score = 209 bits (532), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 118/341 (34%), Positives = 196/341 (57%), Gaps = 8/341 (2%) Query: 3 KQSIYGLTLEQMASWLVEHGHKKSRASRIWESLYRQRVKDFAEMTGVNGETVQLLSEHFV 62 K ++ GLT Q+ + G K+ RA ++ + ++ V DF MT V + L Sbjct: 8 KVNLLGLTQPQLEQFFESIGEKRFRAGQVMKWIHHFGVDDFDAMTNVGKALREKLKASAE 67 Query: 63 FQTMDEHVKQESADGTIKFLFRLKDGNLIETVLMRQKYGLSVCVTTQVGCNIGCSFCASG 122 + + + SADGT K++ R+ G+ +ETV + Q ++CV++Q GC + CSFC++G Sbjct: 68 IRGPEIVSQDISADGTRKWVVRVASGSCVETVYIPQGGRGTLCVSSQAGCALDCSFCSTG 127 Query: 123 LLAKSRDLSSGEIVEQIMKVQHHLDGLG--LGQRVSHVVVMGIGEPFDNFTNLLNFLTII 180 + DL++ E++ Q+ + + + +++VV+MG+GEP NF N++ + I+ Sbjct: 128 KQGFNSDLTAAEVIGQVWIANKSFGTVPAKIDRAITNVVMMGMGEPLLNFDNVVAAMNIM 187 Query: 181 KDHKGLAIAGKGITVSTSGLADKIREFADANMQVNLAISLHAPNNELRTRIMKINRAIPI 240 D G I+ + +T+STSG+ I + + + V+LA+SLHAPN+ELR +++ IN+ P+ Sbjct: 188 MDDLGYGISKRKVTLSTSGVVPMIDKLGEV-IDVSLALSLHAPNDELRNKLVPINKKYPL 246 Query: 241 EKLMPAIDYYLEK--TNRRITLEYILLKDVNDRQEHALELAELIGGRRQMANVNLIPYNP 298 L+ A Y+ + R +T+EY LLKDVND+ EHA ++ L+ + +NLIP+NP Sbjct: 247 GMLLDACRRYISRLGEKRVLTVEYTLLKDVNDQPEHAEQMIALL--KDTPCKINLIPFNP 304 Query: 299 VDEHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVDIDA 339 HS Y+R ++R F D L K G +V+ R G DIDA Sbjct: 305 F-PHSGYERPSNNAIRRFQDMLHKGGFNVTVRTTRGDDIDA 344 >PA1839 [R] COG0820 Predicted Fe-S-cluster redox enzyme Length = 346 Score = 130 bits (326), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 94/303 (31%), Positives = 149/303 (49%), Gaps = 18/303 (5%) Query: 37 RQRVKDFAEMTGVNGETVQLLSEHFVFQTMDEHVKQESADGTIKFLFRLKDGNLIETVLM 96 RQR +DF + GV Q+ +E + H + ++DG+ + L L D ++E+VL+ Sbjct: 39 RQRAEDFLPL-GVRHGLPQVAAELEGIARL--HSEHPASDGSSRLLVELADRQMVESVLL 95 Query: 97 RQKYGLSVCVTTQVGCNIGCSFCASGLLAKSRDLSSGEIVEQIMKVQHHLDGLGLGQRVS 156 + GL CV+TQVGC +GC FC +G R + S E+V Q++ + + V Sbjct: 96 -PRGGL--CVSTQVGCAVGCVFCMTGRSGLLRQVGSLEMVAQVVLARRR-------RAVK 145 Query: 157 HVVVMGIGEPFDNFTNLLNFLTIIKDHKGLAIAGKGITVSTSGLADKIREFADANMQVNL 216 VV MG+GEP N N+L + ++ G I K + ST G ++ L Sbjct: 146 KVVFMGMGEPAHNLDNVLEAIDLLGTDGG--IGHKNLVFSTVGDPRVFERLPRQRVKPAL 203 Query: 217 AISLHAPNNELRTRIMKINRAIPIEKLMPAIDYYLEKTNRRITLEYILLKDVNDRQEHAL 276 A+SLH+ ELR +++ + E+L+ A + Y + + I ++ LL+ +ND E Sbjct: 204 ALSLHSTRAELRRQLLPKAPPLSPEELVEAGEAYARRVDYPIQYQWTLLEGINDSLEEMD 263 Query: 277 ELAELIGGRRQMANVNLIPYNPVDEHSQYQRSERESVRAFFDTLKKQGVSVSTRLEHGVD 336 + L+ GR A +NLIPYN +D + Y+R E + L +GV R G D Sbjct: 264 GILRLLKGR--FAVMNLIPYNSMDGDA-YRRPSGERIVELVRYLHSRGVLTKVRNSAGQD 320 Query: 337 IDA 339 ID Sbjct: 321 IDG 323 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,423,155 Number of extensions: 965161 Number of successful extensions: 9830 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 9807 Number of HSP's successfully gapped: 8 Length of query: 339 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 232 Effective length of database: 26,037,693 Effective search space: 6040744776 Effective search space used: 6040744776 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)