BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0045.0005.p (118 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurQ [L] COG0322 Nuclease subunit of the excinuclease complex 159 1e-39 >BS_yurQ [L] COG0322 Nuclease subunit of the excinuclease complex Length = 124 Score = 159 bits (401), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 79/124 (63%), Positives = 96/124 (77%), Gaps = 6/124 (4%) Query: 1 MISITIPAPDVTIMKQEN------PVLSHIYGFTDFHLITREKGGIFMFYNDKDELLFVG 54 MI I IPAP+VTI +++ P + I+GF DFH I R+KGGIFMFYN DELLFVG Sbjct: 1 MIKIDIPAPNVTITERQQSANDNEPKIKPIHGFIDFHQIPRDKGGIFMFYNIHDELLFVG 60 Query: 55 KARKLRPRIKKHFEDNVSVMKPHRDEVAKIEVCIIDDAVDREIYETYIVNKLRAKYNVEK 114 KARKLR RIKKHFED VS +K HRDEV KIEVC++DD ++REIYETYI+N +KYN++K Sbjct: 61 KARKLRQRIKKHFEDTVSPIKHHRDEVYKIEVCVVDDPMEREIYETYIINTQHSKYNIDK 120 Query: 115 VLYK 118 V +K Sbjct: 121 VFFK 124 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.142 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,817,742 Number of extensions: 337257 Number of successful extensions: 533 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 535 Number of HSP's successfully gapped: 1 Length of query: 118 Length of database: 38,378,431 Length adjustment: 85 Effective length of query: 33 Effective length of database: 28,575,041 Effective search space: 942976353 Effective search space used: 942976353 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 109 (46.6 bits)