BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0015.p (268 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 276 2e-74 SA0021 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 270 1e-72 CT738 [R] COG1235 Metal-dependent hydrolases of the beta-lactama... 164 1e-40 CPn0879 [R] COG1235 Metal-dependent hydrolases of the beta-lacta... 162 4e-40 PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactam... 153 3e-37 >lin0319 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Length = 252 Score = 276 bits (706), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 0/240 (0%), Positives = 0/240 (0%) Query: 3 ISFTVLSSGSTGNVTVVRNGETTLMIDAGLSAKRIDELLAMRELTGQDIDGILVTHEHSD 62 L G S + + +TG D ++ S Sbjct: 15 ERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSA 74 Query: 63 HIKGLGAMARKYDLPIYANTNTWGAIEKGIGNIAEQNRVVLETGQYRDFGSLRVESFAIS 122 A + + A G L V+ Sbjct: 75 LPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNW 134 Query: 123 HDAAEPVGYNFYDGKEKLCVATDLGYVSDKVRTAISDADVLVLEANHDIEMLRMGRYPWN 182 + + + + +S+A V Sbjct: 135 LEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAII 194 Query: 183 TKRRILGDLGHLSNEAAGAALSEILTGRTKRTYLAHLSRDHNMMDLAKMSVRGAMEDRGC 242 Sbjct: 195 254 >SA0021 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Length = 252 Score = 270 bits (691), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 0/259 (0%), Positives = 0/259 (0%), Gaps = 1/259 (0%) Query: 3 ISFTVLSSGSTGNVTVVRNGETTLMIDAGLSAKRIDELLAMRELTGQDIDGILVTHEHSD 62 L + + V + + A L Q +T S Sbjct: 5 NPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSS 64 Query: 63 HIKGLGAMARKYDLPIYANTNTWGAIEKGIGNIAEQNRVVLETGQYRDFGSLRVESFAIS 122 A + D + + I + Y F L Sbjct: 65 PAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQG 124 Query: 123 HDAAEPVGYNFYDGKEKLCVATDLGYVSDKVRTAISDADVLVLEANHDIEMLRMGRYPWN 182 A + + D+ R ++ + + + R + Sbjct: 125 VLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYY 184 Query: 183 TKRRILGDLGHLSNEAAGAALSEILTGRTKRTYLAHLSRDHNMMDLAKMSVRGAMEDRGC 242 + + + LT ++ +L + + + + Sbjct: 185 HQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENG-QILLAFPR 243 Query: 243 FFKDSEFRLCDTYYDRPTP 261 Sbjct: 244 262 >CT738 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Length = 252 Score = 164 bits (415), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 14/235 (5%), Positives = 33/235 (14%), Gaps = 2/235 (0%) Query: 5 FTVLSSGSTGNVTVVRNGETTLMIDAGLSAKRIDELLAMRELTGQDIDGILVTHEHSDHI 64 + E + D T Sbjct: 4 WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDS 63 Query: 65 KGLGAMARKYDLPIYANTNTWGAIEKGIGNIAEQNRVVLETGQYRDFGSLRVESFAISHD 124 + LP + + + + Q+ + Sbjct: 64 SPAMIAEARSALPDCQFVEADIRNWQPV-QALDLIFA-NASLQWLPDHYELFPHLVSLLN 121 Query: 125 AAEPVGYNFYDGKEKLCVATDLGYVSDKVRTAISDADVLVLEANHDIEMLRMGRYPWNTK 184 + D + ++ + + A +DI Sbjct: 122 PQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRT 181 Query: 185 RRILGDLGHLSNEAAGAALSEILTGRTKRTYLAHLSRDHNMMDLAKMSVRGAMED 239 H + A + L L + Sbjct: 182 TYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ 236 >CPn0879 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Length = 252 Score = 162 bits (411), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 14/234 (5%), Positives = 33/234 (14%), Gaps = 4/234 (1%) Query: 5 FTVLSSGSTGNVTVVRNGETTLMIDAGLSAKRID-ELLAMRELTGQDIDGILVTHEHSDH 63 + E + D T Sbjct: 4 WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN-STALLQQRWPAARITGID 62 Query: 64 IKGLGAMARKYDLPIYANTNTWGAIEKGIGNIAEQNRVVLETGQYRDFGSLRVESFAISH 123 + LP + + + + Q+ Sbjct: 63 SSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIF--ANASLQWLPDHYELFPHLVSLL 120 Query: 124 DAAEPVGYNFYDGKEKLCVATDLGYVSDKVRTAISDADVLVLEANHDIEMLRMGRYPWNT 183 + + D + ++ + + A +DI Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 184 KRRILGDLGHLSNEAAGAALSEILTGRTKRTYLAHLSRDHNMMDLAKMSVRGAM 237 H + A + L L + Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQEN 234 >PA1012 [R] COG1235 Metal-dependent hydrolases of the beta-lactamase superfamily I Length = 252 Score = 153 bits (386), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 12/230 (5%), Positives = 32/230 (13%), Gaps = 5/230 (2%) Query: 3 ISFTVLSSGSTGNVTVVRNGETTLMIDAGLSAKRIDELLAMRELTGQDIDGILVTHEHSD 62 +S S + R L + + + +L + + + Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 63 HIKGLGAMARKYDLPIYANTNTWGAIEKGIGNIAEQNRVVLETGQYRDFGSLRVESFAIS 122 +A L Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQAL-----DLIFANASLQWLPDHYELFPH 115 Query: 123 HDAAEPVGYNFYDGKEKLCVATDLGYVSDKVRTAISDADVLVLEANHDIEMLRMGRYPWN 182 + + + + A + Sbjct: 116 LVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCE 175 Query: 183 TKRRILGDLGHLSNEAAGAALSEILTGRTKRTYLAHLSRDHNMMDLAKMS 232 + + A R L + + +M Sbjct: 176 VDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQML 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,430,349 Number of extensions: 825470 Number of successful extensions: 1972 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1988 Number of HSP's successfully gapped: 10 Length of query: 268 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 163 Effective length of database: 26,268,361 Effective search space: 4281742843 Effective search space used: 4281742843 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)