BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0014.p (414 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yyxA [O] COG0265 Trypsin-like serine proteases, typically per... 267 3e-71 lin0320 [O] COG0265 Trypsin-like serine proteases, typically per... 264 3e-70 BH4022 [O] COG0265 Trypsin-like serine proteases, typically peri... 251 1e-66 BS_htrA [O] COG0265 Trypsin-like serine proteases, typically per... 227 2e-59 SA1549 [O] COG0265 Trypsin-like serine proteases, typically peri... 224 2e-58 >BS_yyxA [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 400 Score = 267 bits (682), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 162/395 (41%), Positives = 242/395 (61%), Gaps = 37/395 (9%) Query: 33 PRRSRRGLATWQVAMICSVVSAVTAVLLFSLVTGQLTHEKAESPAV-----------IGK 81 P+RS++G + S++ + +L + + L++E ++ A+ I Sbjct: 16 PKRSKKGY------FLSSLIGVIVGAVLMAFIMPYLSNEGLDTGALDQQQNNNGRESIRT 69 Query: 82 VAASSGDPYDRIIQAAALVRPAVVSIINHKEDNKELNILDESA---LGSGVIYKKDDGKA 138 V S + +I+ + PAVV ++N ++ + I ES GSGVIYKK+D A Sbjct: 70 VNVSVNNAVTKIVSNMS---PAVVGVVNIQKSD----IWGESGEAGSGSGVIYKKNDHSA 122 Query: 139 FIITNNHVIEGAGKLEVVTVNGETRKATLVGADKVSDIAVLSIDAEGIKTVAQIGDSSKL 198 +++TN+HVIEGA ++E+ +G A LVG+D++ D+AVL + ++ IK VA G+S K+ Sbjct: 123 YVVTNHHVIEGASQIEISLKDGSRVSADLVGSDQLMDLAVLRVKSDKIKAVADFGNSDKV 182 Query: 199 RLGETVIAIGNPLGL--GDTLTSGIVSYTERTIPVSLNQDGVYDWEQEVIQTDAAINEGN 256 + GE VIAIGNPLGL ++T G++S TER IPV N DG DW EV+QTDAAIN GN Sbjct: 183 KSGEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINPGN 242 Query: 257 SGGALVDLNGKVIGINTMKISDTGVEGLGFAIPANHVMETADELAAKGKIARSYLGVYSV 316 SGGAL++++GKVIGIN+MKI+++ VEG+G +IP+ V+ ++L GK+ R +LG+ Sbjct: 243 SGGALLNMDGKVIGINSMKIAESAVEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEMK 302 Query: 317 DLNNPYVPLAEDQRKE-LNLPDSVKDGVVVL--DAVGPAKVAGLQLNDVITKFDNQSITS 373 L++ +A E L LP +V +G VV+ DA PA AGL+ DVIT+FD + Sbjct: 303 SLSD----IASYHWDETLKLPKNVTNGAVVMGVDAFSPAGKAGLKELDVITEFDGYKVND 358 Query: 374 TLSLRKYLYDHTKIGDELKITFYRNGEVKQVTVKL 408 + LRK LY K+GD +K+ FYR G+ K V +KL Sbjct: 359 IVDLRKRLY-QKKVGDRVKVKFYRGGKEKSVDIKL 392 >lin0320 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 499 Score = 264 bits (674), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 150/325 (46%), Positives = 214/325 (65%), Gaps = 13/325 (4%) Query: 93 IIQAAALVRPAVVSIINHKEDNK---ELNILDESALGSGVIYKKDDGKAFIITNNHVIEG 149 + +A V+ AVVS++N++ + + E++ GSGVIYKK +GKA+I+TNNHV+ Sbjct: 172 VTKAVDKVQDAVVSVLNYQSSSSLDGTTSSEKEASSGSGVIYKKANGKAYIVTNNHVVAD 231 Query: 150 AGKLEVVTVNGETRKATLVGADKVSDIAVLSIDAEGIKTVAQIGDSSKLRLGETVIAIGN 209 A KLEV NG+ +A L+G D+ +D+AVL ID + + TVA GDS L+LGE IAIG+ Sbjct: 232 ANKLEVTFTNGKKSEAKLLGTDEWNDLAVLEIDDKNVSTVATFGDSDSLKLGEPAIAIGS 291 Query: 210 PLG--LGDTLTSGIVSYTERTIPVSLNQDGVYDWEQEVIQTDAAINEGNSGGALVDLNGK 267 PLG ++T GI+S R +PV N DG DWE +VIQTDAAIN GNSGGAL+++ G+ Sbjct: 292 PLGTEFSGSVTQGIISGLNRAVPVDTNGDGTEDWEADVIQTDAAINPGNSGGALINIEGQ 351 Query: 268 VIGINTMKISDTGVEGLGFAIPANHVMETADELAAKGKIARSYLGVYSVDLNNPYVPLAE 327 VIGIN+MKIS VEG+ FAIP+N V ++L KG++ R LGV D++ + E Sbjct: 352 VIGINSMKISMENVEGISFAIPSNTVEPIIEQLETKGEVERPSLGVSLRDVDT----IPE 407 Query: 328 DQRKE-LNLPDSVKDGVVVLDAV--GPAKVAGLQLNDVITKFDNQSITSTLSLRKYLY-D 383 Q+K L LPDSV G +V V A AGL+ DVI + + + +T++++LRK LY + Sbjct: 408 TQQKNILKLPDSVDYGAMVQQVVSGSAADKAGLKQYDVIVELNGEKVTNSMTLRKILYGN 467 Query: 384 HTKIGDELKITFYRNGEVKQVTVKL 408 KIGD++K+ +YR+G+ + +KL Sbjct: 468 DVKIGDKVKVKYYRDGKEQSTDIKL 492 >BH4022 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 406 Score = 251 bits (641), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 157/352 (44%), Positives = 222/352 (63%), Gaps = 16/352 (4%) Query: 65 TGQLTHEKAESPAVIGKV-AASSGDPYDRIIQAAALVRPAVVSIINHKEDNKELNILDES 123 TGQLT E+P I V A + D + QA V AVV I++ + + +E Sbjct: 63 TGQLT----EAPNDIETVNYAVNSD----VSQAVEKVSDAVVGIVSMTNGSMFSSSEEEE 114 Query: 124 ALGSGVIYKKDDGKAFIITNNHVIEGAGKLEVVTVNGETRKATLVGADKVSDIAVLSIDA 183 GSGVIYKK+ +AFI+TN HVI GA ++EVV +G A ++G+D +D+AVL ID Sbjct: 115 GTGSGVIYKKEGDRAFIVTNEHVISGANQVEVVLTDGSRLPAEVLGSDVFTDLAVLEIDG 174 Query: 184 EGIKTVAQIGDSSKLRLGETVIAIGNPLGL--GDTLTSGIVSYTERTIPVSLNQDGVYDW 241 ++TVA+ G+S L GE IAIGNPLGL ++T GI+S TER+IP+ L +G DW Sbjct: 175 SDVETVAEFGNSDLLSPGEPAIAIGNPLGLRFSSSVTLGIISATERSIPIDLTGNGQIDW 234 Query: 242 EQEVIQTDAAINEGNSGGALVDLNGKVIGINTMKISDTGVEGLGFAIPANHVMETADELA 301 + EV+QTDAAIN GNSGGALV++ G+VIGIN+MKI+ + VEG+GFAIP+N + ++L Sbjct: 235 QAEVLQTDAAINPGNSGGALVNIQGQVIGINSMKIAQSTVEGIGFAIPSNLAIPVIEDLE 294 Query: 302 AKGKIARSYLGVYSVDLNNPYVPLAEDQRKELNLPDSVKDGVVVLDAV--GPAKVAGLQL 359 G + R +GV L+ +P + + L LP+ VK GVV+ D V PA+ AGL+ Sbjct: 295 FYGDVQRPQMGVAFRSLSE--IP-SFHWEETLKLPEDVKGGVVITDIVPMSPAETAGLRQ 351 Query: 360 NDVITKFDNQSITSTLSLRKYLYDHTKIGDELKITFYRNGEVKQVTVKLLEK 411 DVI + + + I LRK+LY IGDE+++T+YR G+ + T+ L+E+ Sbjct: 352 YDVIVELNGEDINDGHELRKFLYTELNIGDEVEVTYYREGKKETTTLTLVEQ 403 >BS_htrA [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 449 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 139/294 (47%), Positives = 188/294 (63%), Gaps = 11/294 (3%) Query: 126 GSGVIYKKDDGKAFIITNNHVIEGAGKLEVVTVNGETRKATLVGADKVSDIAVLSIDAEG 185 GSGVI+KK++GKA+IITNNHV+EGA L+V +G A LVG+D ++D+AVL I + Sbjct: 160 GSGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDH 219 Query: 186 IKTVAQIGDSSKLRLGETVIAIGNPLG--LGDTLTSGIVSYTERTIPVSLNQDGVYDWEQ 243 + VA GDSS LR GETVIAIG+PLG L T+T GIVS +RT+ +S + + Sbjct: 220 VTKVANFGDSSDLRTGETVIAIGDPLGKDLSRTVTQGIVSGVDRTVSMSTS---AGETSI 276 Query: 244 EVIQTDAAINEGNSGGALVDLNGKVIGINTMKISDTGVEGLGFAIPANHVMETADELAAK 303 VIQTDAAIN GNSGG L++ +GK++GIN+MKIS+ VEG+GFAIP+N V A+EL +K Sbjct: 277 NVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFAIPSNDVKPIAEELLSK 336 Query: 304 GKIARSYLGVYSVDLNNPYVPLAEDQRKELNLPDS-VKDGVVVLDAV--GPAKVAGLQLN 360 G+I R Y+GV +DL VP Q L L S + GV + + PA+ AGL+ Sbjct: 337 GQIERPYIGVSMLDLEQ--VP-QNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAE 393 Query: 361 DVITKFDNQSITSTLSLRKYLYDHTKIGDELKITFYRNGEVKQVTVKLLEKPEE 414 D+I + I + LR LY KIGD +++ RNG+ +KL +K E+ Sbjct: 394 DIIIGLKGKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEK 447 >SA1549 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 424 Score = 224 bits (570), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 149/409 (36%), Positives = 234/409 (57%), Gaps = 43/409 (10%) Query: 30 HKWPRRSRRGLATWQVAMICSVVSAVTAVLLFSLV--------------TGQLTHEKAES 75 H R RR W +I ++++ + LL + T Q T+ K Sbjct: 14 HSQTPRYRRPKFPWFKTVIVALIAGIIGALLVLGIGKVLNSTILNKDGSTVQTTNNKG-- 71 Query: 76 PAVIGKVAASSGDPYDRIIQAAALVRPAVVSIINHKEDNKELNIL-------DESALGSG 128 G + + + V P +V +IN ++ + ++L E+ +GSG Sbjct: 72 ----GNQLDGQSKKFGTVHEMIKSVSPTIVGVINMQKASSVDDLLKGKSSKPSEAGVGSG 127 Query: 129 VIYKKDDGKAFIITNNHVIEGAGKLEVVTVNGETRKATLVGADKVSDIAVLSID-AEGIK 187 VIY+ ++ A+I+TNNHVI+GA ++ V N + KA LVG D V+DIAVL I+ +GIK Sbjct: 128 VIYQINNNSAYIVTNNHVIDGANEIRVQLHNKKQVKAKLVGKDAVTDIAVLKIENTKGIK 187 Query: 188 TVAQIGDSSKLRLGETVIAIGNPLGL--GDTLTSGIVSYTERTIPVSLNQDGVYDWEQEV 245 + Q +SSK++ G++V A+GNPLGL +++TSGI+S +ERTI + + V Sbjct: 188 AI-QFANSSKVQTGDSVFAMGNPLGLQFANSVTSGIISASERTIDAETTGG---NTKVSV 243 Query: 246 IQTDAAINEGNSGGALVDLNGKVIGINTMKISDTGVEGLGFAIPANHVMETADELAAKGK 305 +QTDAAIN GNSGGALVD+NG ++GIN+MKI+ T VEG+GFAIP+N V T ++L GK Sbjct: 244 LQTDAAINPGNSGGALVDINGNLVGINSMKIAATQVEGIGFAIPSNEVKVTIEQLVKHGK 303 Query: 306 IARSYLGVYSVDLNNPYVPLAEDQRKELNLPDSVKDGVVVLDAVGPAKVAGLQLNDVITK 365 I R +G+ ++L + + E++R++L+ +DG+ V A L+ D+IT+ Sbjct: 304 IDRPSIGIGLINLKD----IPEEEREQLHT--DREDGIYVAKADSDID---LKKGDIITE 354 Query: 366 FDNQSITSTLSLRKYLYDHTKIGDELKITFYRNGEVKQVTVKLLEKPEE 414 D + I + LR YLY++ K G+ + +T R+G+ K+V VKL ++ E+ Sbjct: 355 IDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKEVKVKLKQQKEQ 403 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,518,328 Number of extensions: 1355234 Number of successful extensions: 3244 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 2923 Number of HSP's successfully gapped: 98 Length of query: 414 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 305 Effective length of database: 25,807,025 Effective search space: 7871142625 Effective search space used: 7871142625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)