BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0034.p (262 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE0797 [IQR] COG1028 Dehydrogenases with different specificitie... 174 1e-43 SSO2276 [IQR] COG1028 Dehydrogenases with different specificitie... 173 2e-43 PH1901 [IQR] COG1028 Dehydrogenases with different specificities... 165 8e-41 APE0912 [IQR] COG1028 Dehydrogenases with different specificitie... 164 2e-40 PAB2177 [IQR] COG1028 Dehydrogenases with different specificitie... 160 2e-39 >PAE0797 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 97/253 (38%), Positives = 139/253 (54%) Query: 5 LTGKSVFVAAASKGLGLATALEFAREGAKVTIASRNLEQLEAARRAIAEATGREVAVVQM 64 L + V AAS+G+G A AREG+ + IA+RNLE+LE + ++ G V VQ Sbjct: 3 LKDRVSIVTAASRGIGAGVATVLAREGSNLVIAARNLERLEGLAKQLSSQYGVSVVPVQA 62 Query: 65 DVNRPEEIGRAIEAAADYGGGLDVLVTNAGGPPGGGFEEMTDADWSGGYELTLMGTVRMI 124 D+ R E++ + ++ A + G +D+L N G P G F E+T+ DW G L LM V + Sbjct: 63 DLTRREDVRKIVDEALRHFGKVDILAYNTGPPKPGTFLELTEDDWDYGVRLLLMSAVWIT 122 Query: 125 REALPYLXXXXXXXXXXXXXXXXKQPIGGLILSNVFRAGVSALFKTLASELAPEGILINS 184 ++ LPY+ KQPI L LSNV R ++ L KTLA +L IL+N Sbjct: 123 KDVLPYMIERRWGRLIYITSSTLKQPIPTLTLSNVIRISLAGLVKTLAYQLGKYNILVNG 182 Query: 185 LAPGRIGTDRILQLDSKRADARGISREQVEQEALAQIPAGRSGTPEEFGKAAVFLGSFAN 244 + G I T+R+ ++ RA G S +++ +E +IP GR G PEE G+ FL S Sbjct: 183 IMQGYIDTERVREVAEARAKREGRSLDEILRELEREIPLGRIGKPEEIGELVAFLASEKA 242 Query: 245 TYITGQSLLIDGG 257 +YITG +LIDGG Sbjct: 243 SYITGSLILIDGG 255 >SSO2276 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 261 Score = 173 bits (439), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 98/260 (37%), Positives = 137/260 (52%), Gaps = 2/260 (0%) Query: 1 MDLGLTGKSVFVAAASKGLGLATALEFAREGAKVTIASRNLEQLEAARRAIAEATGREVA 60 MDLG+ GK V V AASKG+GLA A F EGAKV I+S + L A + E V+ Sbjct: 1 MDLGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSNDESNLMKAYNKLKEIHSENVS 60 Query: 61 VVQMDVNRPEEIGRAIEAAADYGGGLDVLVTNAGGPPGGGFEEMTDADWSGGYELTLMGT 120 ++ D+ P E+ + GGLDVL G P G ++D DW + L L+ Sbjct: 61 YIKADLMNPHEVKSLVHEGYRKLGGLDVLAYVTGSPKPGNLFSLSDKDWMDAFNLLLLSA 120 Query: 121 VRMIREALPYLXXXXXXXXXXXXXXXXKQPIGGLILSNVFRAGVSALFKTLASELAPEGI 180 V +REA + +QPI L LSNV R ++ L K + EL P+GI Sbjct: 121 VVAVREAGQLMQSGGRIILSTSTTL--RQPIDNLDLSNVVRLSLAGLIKVASRELGPKGI 178 Query: 181 LINSLAPGRIGTDRILQLDSKRADARGISREQVEQEALAQIPAGRSGTPEEFGKAAVFLG 240 L+N + PG T+R+LQ+ RA G S ++V + +A +P GR+G PEE +FL Sbjct: 179 LVNGVMPGWTKTERLLQIIRDRAIREGKSEDEVLKSIVADVPLGRAGEPEEIANVVLFLA 238 Query: 241 SFANTYITGQSLLIDGGMVK 260 S +TY+TG + +DGG +K Sbjct: 239 SNLSTYVTGALIPVDGGSIK 258 >PH1901 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 282 Score = 165 bits (417), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 97/262 (37%), Positives = 136/262 (51%), Gaps = 1/262 (0%) Query: 1 MDLGLTGKSVFVAAASKGLGLATALEFAREGAKVTIASRNLEQLEAARRAIAEATGREVA 60 + + L+GK F A+SKG+G A AR GA V I SRN E L+ A+ I + EV Sbjct: 21 LRIDLSGKLAFTTASSKGIGFGVARVLARAGADVIILSRNEENLKRAKEKIKSESNVEVH 80 Query: 61 VVQMDVNRPEEIGRAIEAAADYGGGLDVLVTNAGGPPGGGFEEMTDADWSGGYELTLMGT 120 + D+ + E++ R I+ D G D+ + GGP G F EM+ DW G +L L Sbjct: 81 YIVADLTKMEDLERTIKELKDIGDP-DIFFFSTGGPKPGYFMEMSMEDWEGAVKLLLYPA 139 Query: 121 VRMIREALPYLXXXXXXXXXXXXXXXXKQPIGGLILSNVFRAGVSALFKTLASELAPEGI 180 V + + +P + K+PI + LSNV R ++ L +TLA EL P+GI Sbjct: 140 VYLTKALVPGMERKGFGRIVYSTSVAIKEPIPNIALSNVVRIAMAGLVRTLAKELGPKGI 199 Query: 181 LINSLAPGRIGTDRILQLDSKRADARGISREQVEQEALAQIPAGRSGTPEEFGKAAVFLG 240 +N + PG I TDR++QL RA G S E+ +E IP GR G PEE G FL Sbjct: 200 TVNGIMPGIIRTDRVIQLAQDRAKREGKSVEEALKEYAKPIPLGRLGEPEEIGYLVAFLA 259 Query: 241 SFANTYITGQSLLIDGGMVKSL 262 S +YI G + +DGG + S+ Sbjct: 260 SELGSYINGAMIPVDGGRLNSV 281 >APE0912 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 164 bits (414), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 102/258 (39%), Positives = 137/258 (53%), Gaps = 2/258 (0%) Query: 1 MDLGLTGKSVFVAAASKGLGLATALEFAREGAKVTIASRNLEQLEAARRAIAE-ATGREV 59 MDLG+ GK V A S GLG A+ALE AR GA++ + SRN E+LEAA IA +G +V Sbjct: 1 MDLGIQGKLAVVTAGSSGLGFASALELARNGARLLLFSRNREKLEAAASRIASLVSGAQV 60 Query: 60 AVVQMDVNRPEEIGRAIEAAADYGGGLDVLVTNAGGPPGGGFEEMTDADWSGGYELTLMG 119 +V D+ P +I R E A D GG D+LV + GGP G F E+ DW Y L Sbjct: 61 DIVAGDIREPGDIDRLFEKARDLGGA-DILVYSTGGPRPGRFMELGVEDWDESYRLLARS 119 Query: 120 TVRMIREALPYLXXXXXXXXXXXXXXXXKQPIGGLILSNVFRAGVSALFKTLASELAPEG 179 V + R A + +P L LSN+ R V + +TLA ELAP G Sbjct: 120 AVWVGRRAAEQMVEKGWGRMVYIGSVTLLRPWQDLALSNIMRLPVIGVVRTLALELAPHG 179 Query: 180 ILINSLAPGRIGTDRILQLDSKRADARGISREQVEQEALAQIPAGRSGTPEEFGKAAVFL 239 + +N++ P I TDR+ L +RA GI+ E+ + ++IP GR G PEE FL Sbjct: 180 VTVNAVLPSLILTDRVRSLAEERARRSGITVEEALKSMASRIPMGRVGKPEELASVVAFL 239 Query: 240 GSFANTYITGQSLLIDGG 257 S ++ITG + +DGG Sbjct: 240 ASEKASFITGAVIPVDGG 257 >PAB2177 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 263 Score = 160 bits (405), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 93/262 (35%), Positives = 136/262 (51%), Gaps = 1/262 (0%) Query: 1 MDLGLTGKSVFVAAASKGLGLATALEFAREGAKVTIASRNLEQLEAARRAIAEATGREVA 60 + + L+GK F A+SKG+G A A+ GA V I SRN E L+ AR I E + EV Sbjct: 2 LKIDLSGKLAFTTASSKGIGFGVARVLAKAGADVIILSRNEENLKRAREKIKEESNVEVH 61 Query: 61 VVQMDVNRPEEIGRAIEAAADYGGGLDVLVTNAGGPPGGGFEEMTDADWSGGYELTLMGT 120 + D+ + +++ R ++ + G D+ + GGP G F EM+ DW +L L Sbjct: 62 YIVADLTKMDDLERTVKELKNIGEP-DIFFFSTGGPKPGYFMEMSMEDWEEAVKLLLYPA 120 Query: 121 VRMIREALPYLXXXXXXXXXXXXXXXXKQPIGGLILSNVFRAGVSALFKTLASELAPEGI 180 + + + +P + K+PI + LSNV R ++ L +TLA EL P+GI Sbjct: 121 IYLTKALVPAMERKGFGRIIYSTSVAIKEPIPNIALSNVVRIAMAGLVRTLAKELGPKGI 180 Query: 181 LINSLAPGRIGTDRILQLDSKRADARGISREQVEQEALAQIPAGRSGTPEEFGKAAVFLG 240 +N + PG I TDR++QL RA G + E+ QE IP GR G PEE G FL Sbjct: 181 TVNGIMPGIIRTDRMIQLAQDRAKREGKTVEEALQEYAKPIPLGRLGEPEEIGYLVAFLA 240 Query: 241 SFANTYITGQSLLIDGGMVKSL 262 S +YI G + +DGG + S+ Sbjct: 241 SDLGSYINGAMIPVDGGRLNSV 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,004,792 Number of extensions: 739526 Number of successful extensions: 2729 Number of sequences better than 1.0e-05: 256 Number of HSP's gapped: 2291 Number of HSP's successfully gapped: 259 Length of query: 262 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 158 Effective length of database: 26,383,695 Effective search space: 4168623810 Effective search space used: 4168623810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)