BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0029.p (237 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA3051 [R] COG1011 Predicted hydrolase (HAD superfamily) 100 2e-21 MA3043 [R] COG1011 Predicted hydrolase (HAD superfamily) 80 3e-15 VCA0608 [R] COG1011 Predicted hydrolase (HAD superfamily) 64 2e-10 PAB1316 [R] COG1011 Predicted hydrolase (HAD superfamily) 62 8e-10 SPy1066 [R] COG1011 Predicted hydrolase (HAD superfamily) 60 3e-09 >MA3051 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 217 Score = 100 bits (249), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 68/207 (32%), Positives = 107/207 (51%), Gaps = 6/207 (2%) Query: 6 IFDLYGTLIDIETDEERPELWERLSLHFSYHGLNISGVELQQRFLRERDGQLAAAGQHCA 65 IFD Y TL+DI+TDE E ER+S Y G+ I L++ + + G+L +GQ Sbjct: 11 IFDCYKTLVDIKTDEGGRETNERVSKWLLYQGVRIEPDRLREEYRWKVIGRLGNSGQQ-- 68 Query: 66 YPDFVMEEVFRAV-ARDLGGNPGQAWLH-ETVRWLRTLSMIKISLYSGVEEILRGLRSRG 123 +PD +EE+F + A + WL ET + RT S+ K+ Y +L R+ Sbjct: 69 HPDIRIEEIFAEICAENAFREIDPFWLGIETAKVFRTASLKKLEAYPQSLRLLEKYRNIP 128 Query: 124 KKVFLLSNGQKTFIEAELTMLGILHLFDGVAISSEAGISKPDPLFYRYLTEKYGADLSSA 183 K + +SN Q+ F E EL LG+ F+ V +SS+ I KPD +R + G + Sbjct: 129 KCI--VSNAQRVFTEQELRFLGLYDRFNFVIMSSDHRIKKPDTRLFRMALDGLGLEPWEV 186 Query: 184 IMIGNDPRTDMAGAAAVGIDSCYIRTA 210 + IG+ P D+ ++G+++ +IR A Sbjct: 187 LSIGDTPENDIYPPQSLGMNAMHIRDA 213 >MA3043 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 254 Score = 80.1 bits (196), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 59/209 (28%), Positives = 103/209 (49%), Gaps = 8/209 (3%) Query: 6 IFDLYGTLIDIETDEERPELWERLSLHFSYHGLNISGVELQQRFLRERDGQLAAAGQHCA 65 IFD Y TLIDI T+E R E +E +S +YHG+ I +L ++ + + ++ + + Sbjct: 42 IFDCYQTLIDIHTEEHRVETYETVSAWLAYHGVKIKPEKLWDTYMFKVEERMDESQE--I 99 Query: 66 YPDFVMEEVFRAVARD--LGGNPGQAWLHETVRWLRTLSMIKISLYSGVEEILRGLRSRG 123 YP+ +EE+F + R+ + + ET R R S+ K+ + +++ + Sbjct: 100 YPEVRVEEIFAEICRENSIWKIDEKIMGIETSRVFRAASIRKLRPFPQSIKLIEHCINIP 159 Query: 124 KKVFLLSNGQKTFIEAELTMLGILHLFDGVAISSEAGISKPDPLFYRYLTEKYGADLS-- 181 K ++SNGQ+ F E EL LGI FD V SS+ G KPD + ++ +L Sbjct: 160 K--CIISNGQRVFSELELRFLGIYDYFDFVIFSSDVGYKKPDLRLFMTALKRMELELEPR 217 Query: 182 SAIMIGNDPRTDMAGAAAVGIDSCYIRTA 210 + +G+ ++ A +G+ + I A Sbjct: 218 CVMSLGDSYENEILPARKLGMRAMTIEEA 246 >VCA0608 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 224 Score = 63.5 bits (153), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 30/108 (27%), Positives = 58/108 (53%), Gaps = 2/108 (1%) Query: 107 SLYSGVEEILRGLRSRGKKVFLLSNGQKTFIEAELTMLGILHLFDGVAISSEAGISKPDP 166 +L G E+++ L+ + + + +++NG + L G+ FD + IS E GI+KPD Sbjct: 95 TLLPGAMELMQALQGKAR-LGIITNGFTELQDVRLAKTGMTDFFDQIVISEEVGIAKPDA 153 Query: 167 LFYRYLTEKYGADLSS-AIMIGNDPRTDMAGAAAVGIDSCYIRTASSP 213 + + E+ G S +M+G++P +D+ G GI++C++ P Sbjct: 154 GIFAHALERMGNPTKSRVLMVGDNPHSDILGGLNFGIETCWLNVHQHP 201 >PAB1316 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 229 Score = 62.0 bits (149), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 61/246 (24%), Positives = 98/246 (39%), Gaps = 38/246 (15%) Query: 1 MYQTYIFDLYGTLIDIETDEERPELWERLSLHFSYHGLNISGVELQQRFLRE------RD 54 M + FD GTL+ E + H + G L R + E R+ Sbjct: 1 MIRAVFFDFVGTLLSSEGE---------AVTHLKIMEEVLKGYNLDPRKILEEYEKLTRE 51 Query: 55 GQLAAAGQHCAYPDFVMEEVFRAVARDLGGNPGQAWLHETVRWLRTLSMIKI--SLYSGV 112 A AG+ + EE+ R ++ + G + + W L M + LY V Sbjct: 52 AFSAYAGKPYRPIRDIEEEIMRKISEEYGFKYPENF------WEIHLKMHQEYGKLYPEV 105 Query: 113 EEILRGLRSRGKKVFLLSNGQKTFIEAELTMLGILHLFDGVAISSEAGISKPDPLFYRYL 172 E+L+ LR + V ++++ ++ A L LG+ LFD + S EAG KP P + Sbjct: 106 VEVLKELR-QSYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIA 164 Query: 173 TEKYGADLSSAIMIGNDPRTDMAGAAAVGIDSCYIRTASSPADVPVQSTVQIWD------ 226 K G A+ +G++P D GA +G+ S + D +WD Sbjct: 165 LRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKD--------LWDRCDFVV 216 Query: 227 GDLRKI 232 DLR++ Sbjct: 217 SDLREV 222 >SPy1066 [R] COG1011 Predicted hydrolase (HAD superfamily) Length = 233 Score = 60.1 bits (144), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 34/122 (27%), Positives = 61/122 (50%), Gaps = 5/122 (4%) Query: 108 LYSGVEEILRGLRSRGKKVFLLSNGQKTFIEAELTMLGILHLFDGVAISSEAGISKPDPL 167 +Y+G +E+L L ++G ++ +NG T + L G+ F + IS ++G KP Sbjct: 104 VYAGAKELLADLTAQGYNLYAATNGIATIQQGRLQASGLAPYFKAIFISEQSGSQKPKKA 163 Query: 168 FYRYLTEK---YGADLSSAIMIGNDPRTDMAGAAAVGIDSCYIRTASSPADVPVQSTVQI 224 FY ++T++ Y D A+MIG+ D+ G G+D+ + + PV T ++ Sbjct: 164 FYDWMTQQVSNYQPD--QALMIGDSLSADVQGGINAGMDTLWYNPKHLLNNSPVHPTYEV 221 Query: 225 WD 226 D Sbjct: 222 SD 223 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,415,833 Number of extensions: 745572 Number of successful extensions: 1667 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 1683 Number of HSP's successfully gapped: 24 Length of query: 237 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 134 Effective length of database: 26,499,029 Effective search space: 3550869886 Effective search space used: 3550869886 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)