BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0022.p (395 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 150 3e-36 PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic... 97 6e-20 SMa2305 [G] COG1653 ABC-type sugar transport system, periplasmic... 87 4e-17 PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic ... 84 4e-16 BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasm... 78 2e-14 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 101/383 (26%), Positives = 179/383 (46%), Gaps = 18/383 (4%) Query: 3 HLWPAGISAQQNKLVGQIIDDYQKDYPNVTIKQEVLENEQYKSKLKVLSASNELPDVGIT 62 H WP + +++ +++KD+P++ I+ E + N+ YK K+KV+ + PD+ + Sbjct: 38 HRWP---KEPEKSYFEEVVKEFEKDHPDIDIQIEAVLNDSYKDKIKVMLGTTSPPDIYFS 94 Query: 63 WAAGFMEPYVKGGLFAPLDDVLDGE-QLKDKFVPGTTEAFVVDGKTYALPIELNISPVYY 121 W+ F +++G L + + V F + K Y +P +++ +Y Sbjct: 95 WSDEFAFKFIRGNKALDLSSYYKNDTDWSSQLVQSQITPFTYENKQYGVPWQMDAKSFFY 154 Query: 122 NKDIFAKYNLQVPATYNEFKHVVKKLSDNGVAPIALGNKDRWTGSLWYMYLADRIAGSDT 181 NKDIF K NL P T++E V KKL ++G PI+ G K WT S + L R+ T Sbjct: 155 NKDIFQKLNLDPPKTWDELIDVSKKLKEHGYTPISFGTKATWTISHYIGTLNQRMVDEKT 214 Query: 182 LKRATNG-TGSFDDPGLIRAAVEVQTLVDMNAFNKGFNGLSNDEGKSEFVNEKAAMYLTG 240 ++ N TG F D G ++A ++Q L M FNK N + ++ + +F + K+AM Sbjct: 215 REKDYNAKTGEFTDEGYVKALEKLQEL--MPYFNKHVNSVDHEYVRQQFKSGKSAMIYAE 272 Query: 241 TWELPNFTTNPDIRQEFKDKVKFFKFPTVDGGKGDINSWVGGPGVGLFVAESSKVKEEAK 300 T E+ P + F FP + G KG + G P G ++ +K +EA Sbjct: 273 TAEIK--LVEP-------VNLGMFPFPEISGQKGSSEALTGAPE-GFMISSRTKHPKEAM 322 Query: 301 AFVEYFVTK-WGQDSVTTAGVIPATKVDTSNSELPHLYVDLLNELNHASSLTLFADVQMK 359 F+++ +K G+ V G A + + + + + + A S+ + D+ + Sbjct: 323 EFLQFLTSKRMGEKLVKDVGKYSAVQGTATEENATAIQREAVQHIVDAKSMVPWFDMDVD 382 Query: 360 PGAAQVHLDMIQALFGKAVTPEQ 382 A +L +Q + G +TP+Q Sbjct: 383 VEVADAYLTGVQQMLGGDMTPQQ 405 >PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 96.7 bits (239), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 93/332 (28%), Positives = 145/332 (43%), Gaps = 32/332 (9%) Query: 10 SAQQNKLVGQIIDDYQKDYPNVTIKQEVLENEQYKSKLKVLSASNELP-DVGITWAAGFM 68 S + + Q++D+YQK PNV + E+ L + + P DV IT F+ Sbjct: 72 SGAEYEYFKQVLDEYQKINPNVEFEYVTRRAEEIAQILPTQLEAKQAPADVVITSFGWFV 131 Query: 69 EPYVKGGLFAPLDDVLDGEQLKDKFVPGTTEAFVVDGKTYALPIELNISP-VYYNKDIFA 127 K G L +V++ +++V G + +GK +A P + + P +Y K F Sbjct: 132 VEMAKRGHVVDLTNVIN----PNEYVTGIFDPVTYEGKIWATPFTIVLKPGFWYRKSFFQ 187 Query: 128 KYNLQVPATYNEFKHVVKKLSDNGVAPIALGNKDRWTGSLWYMYLADRIAGSDTLKRATN 187 ++ L P T++EF ++K+L + GV PIA G+ W S AG D + T Sbjct: 188 QHGLSEPQTWDEFLQLLKRLKEIGVTPIASGDGVGWPLSDVTEAFIIAYAGPDVFRGLTE 247 Query: 188 GTGSFDDPGLIRAAVEVQTLVDMNAFNKGFNGLSND--EGKSEFVNEKAAMYLTGTWELP 245 FDDP + + +L+ +G+ G + + ++ N +Y GTW L Sbjct: 248 KKVKFDDPRINAVFRDRISLL----LREGYFGEPEEWTQAVEKWWNGTYGIYFMGTWIL- 302 Query: 246 NFTTNPDIRQEFKDKVKFFKFPTVDGGKGDINSWVGGPGVGLFVAESSKVKEEAKAFVEY 305 NP D V FF P G G + +FV S V+ EA FV++ Sbjct: 303 GMVDNP-------DDVGFFPMPETKGITGGTDY--------IFVPVYSPVRNEAVEFVQW 347 Query: 306 FVTKWGQD--SVTTAGVIPA-TKVDTSNSELP 334 TK GQ+ S T AG +P+ TK D + P Sbjct: 348 LATK-GQEVHSSTKAGKLPSWTKADPNKIWAP 378 >SMa2305 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 459 Score = 87.4 bits (215), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 86/378 (22%), Positives = 153/378 (40%), Gaps = 29/378 (7%) Query: 9 ISAQQNKLVGQIIDDYQKDYPNVTIKQEVLEN---EQYKSKLKVLSASNELPDVGITWAA 65 ISA+ N +++ D+ + V+ N E +K+ ++ + PDV ++ Sbjct: 80 ISAESNANTAEVLRRIAADFGKAAGTKVVVNNMDHEAHKTAIRNYLVAGA-PDVCFWFSG 138 Query: 66 GFMEPYVKGGLFAPLDDVLDGEQLKDKFVPGTTEAFVVDGKTYALPIELNISPVYYNKDI 125 M +V GLF + D+ + E+ D + T + V+GK Y LP + ++Y KD+ Sbjct: 139 NRMRAFVTRGLFDDISDLFEKEKYAD-VLGATAGSVTVEGKQYGLPTGGTLWGMFYRKDV 197 Query: 126 FAKYNLQVPATYNEFKHVVKKLSDNGVAPIALGNKDRWTGSLWYMYLADRIAGSDTLKRA 185 F ++ L+VP ++++ +K + P+A+G KD W + W+ + RI G D Sbjct: 198 FDEHGLKVPTSWDDLLAFGEKCKSASLTPVAMGTKDLWPAAGWFDQMNLRINGLDKHFAL 257 Query: 186 TNGTGSFDDPGLIRAAVEVQTLVDMNAFNKGFNGLSNDEGKSEFVNEKAAMYLTGTWELP 245 NG ++ D L + L+ F E + ++A M G + Sbjct: 258 MNGEMAYTDETLKPVFEHWEELIKQGFFTPDHTSFGWQEAGAFLAQKRAGMMNLGAFVRA 317 Query: 246 NFTTNPDIRQEFKDKVKFFKFPTVDGGKG-----DINSWVGGPGVGLFVAESSKVKEEAK 300 F RQ+ ++ F FP ++ G G +NS + + ++K K A+ Sbjct: 318 AFP-----RQDLP-QLTFAPFPVIEDGLGRYEEFSLNS--------VHIPTNAKNKPGAR 363 Query: 301 AFVEYFVTKWGQDSVTTAG--VIPATKVDTSNSELPHLYVDLLNELNHASSLTLFADVQM 358 F+ +F + G V P + S L + V+ L + S F D Sbjct: 364 EFLTHFYKPENLAAYLEPGGNVPPRNDLPPSKDPLVNAAVESLKAVAGTSQ---FYDRDT 420 Query: 359 KPGAAQVHLDMIQALFGK 376 P AQ L Q + Sbjct: 421 DPDMAQAGLVGFQEFMAR 438 >PH1214 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 441 Score = 84.0 bits (206), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 66/248 (26%), Positives = 113/248 (45%), Gaps = 12/248 (4%) Query: 1 MMHLWPAGISAQQNKLVGQIIDDYQKDYPNVTIKQEVLENE---QYKSKLKVLSASNELP 57 + H W AG + + + ++K YPN+ IK + K L+ L + + P Sbjct: 46 IYHWWTAG---GEKEAFDALAKKFEKKYPNIKIKANPVSGGGGVNLKMVLQSLMLAGKPP 102 Query: 58 DVGITWAAGFMEPYVKGGLFAPLDDVLDGEQLKDKFVPGTTEAFVVDGKTYALPIELNIS 117 D A M Y+K P+DD+ E +K + + + + YA+PI ++ + Sbjct: 103 DTFQVHAGYEMARYIKANQLTPIDDIWT-EDMKKNYPKVIQQMVIFNNHYYAVPINVHRA 161 Query: 118 PV-YYNKDIFAKYNLQVPA--TYNEFKHVVKKLSDNGVAPIALGNKDRWTGSLWYMYLAD 174 V +YNK IF KY + + T ++ V +KL +NGV P ALG++++W + + + Sbjct: 162 NVIWYNKQIFKKYGIDPSSIKTIDDLFAVAEKLKENGVTPFALGDRNKWPATQVFEVMLV 221 Query: 175 RIAGSDTLKRATNGTGSFDDPGLIRAAVEVQTLVDMNAFNKGFNGLSNDEGKSEFVNEKA 234 + G D ++ NG S +DP L + V+ + N + DE + +A Sbjct: 222 AVGGVDYYEKFINGEISANDPTLKKVLEYFVKYVEYS--NPDHAAKTWDEACALVYKGEA 279 Query: 235 AMYLTGTW 242 AM L G W Sbjct: 280 AMTLMGDW 287 >BMEII0542 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 398 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 80/326 (24%), Positives = 143/326 (43%), Gaps = 25/326 (7%) Query: 7 AGISAQQNKLVGQIIDDYQKDYPNVTIKQEVLENEQYKSKLKV-LSASN--ELPDVGITW 63 A SA+ ++ ++ + P +T+ EV+ + KL +SA +L +G W Sbjct: 20 AEYSAKTGPYFEEVKKAFEAENPGITLNFEVVPWDVLLQKLTTDISAGTNADLSIIGTRW 79 Query: 64 AAGFMEPYVKGGLFAPLDDVLDGEQLKDKFVPGTTEAFVVDGKTYALPIELNISPVYYNK 123 F V+ G+ PLD ++ ++ K +F+ V+DGKTY LPI ++ +YYNK Sbjct: 80 LIDF----VQQGIAEPLDGYMN-DEFKGRFIETFLSPSVLDGKTYGLPIAASVRAMYYNK 134 Query: 124 DIFAKYNLQ-VPATYNEFKHVVKKLSDNGVAPIALGNKDRWTGSLWYMYLADRIAGSDTL 182 D+F K ++ PA ++E K K+ G G + + + Y Y A G++ L Sbjct: 135 DLFEKAGIKNPPANWDELKADAAKIKALGGENYGFGLQGKEIETDVYYYYAMWSYGTEIL 194 Query: 183 KRATNGTGSFDDPGLIRAAVEVQTLVDMNAFNKGFNGLSNDEGKSEFVNEKAAMYLTGTW 242 + +GT PG + AA ++++D G + ++ ++ F K M +T Sbjct: 195 NK--DGTSGLSTPGALEAAKLYKSMIDDGLTQPGVTSYAREDVQNLFKQGKVGMMITA-- 250 Query: 243 ELPNFTTNPDIRQEFKDKVKFFKFPTVDGGKGDINSWVGGPGV--GLFVAESSKVKEEAK 300 P + + K++ K+ G + G GV + + ++SK KEEA Sbjct: 251 --------PFLSNQIKEEAPNLKYGVAAIPAGPTGA-RGTYGVTDSVIMFQNSKNKEEAW 301 Query: 301 AFVEYFVTK-WGQDSVTTAGVIPATK 325 +++ K W G +P K Sbjct: 302 KVLDFLFQKDWRAKFTQNEGFLPVNK 327 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,344,319 Number of extensions: 1408836 Number of successful extensions: 4175 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 4172 Number of HSP's successfully gapped: 42 Length of query: 395 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 287 Effective length of database: 25,922,359 Effective search space: 7439717033 Effective search space used: 7439717033 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)