BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0019.p (461 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yisQ [V] COG0534 Na+-driven multidrug efflux pump 353 4e-97 BH2936 [V] COG0534 Na+-driven multidrug efflux pump 323 4e-88 lin0614 [V] COG0534 Na+-driven multidrug efflux pump 273 4e-73 FN0162 [V] COG0534 Na+-driven multidrug efflux pump 268 1e-71 Cj0619 [V] COG0534 Na+-driven multidrug efflux pump 208 1e-53 >BS_yisQ [V] COG0534 Na+-driven multidrug efflux pump Length = 455 Score = 353 bits (905), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 172/442 (38%), Positives = 276/442 (62%) Query: 1 MNQTKHQEFNLVKLTWPIFLELFLFMLMGSVDTFMIXXXXXXXXXXXXXXNQXXXXXXXX 60 + + + F+L LTWPIF+E+ L+M MG+ DT M+ NQ Sbjct: 11 LKKPADKTFSLFSLTWPIFIEVSLYMFMGNADTLMLSQYSDNSVAAVGVSNQILNLIIVM 70 Query: 61 XXXXGNGAAIVVSQYLGSRKPKEAAEVTGNAVTLNLAVGIIFSTAILVFGEALLRALNVT 120 G +++SQ+LGSR+ KEA EV ++ N + + S + LL + ++ Sbjct: 71 FSFIATGTTVIISQFLGSRQKKEAMEVAYVSIGANFFISLAISAVVFFAAVPLLHMMGLS 130 Query: 121 GDILIHAKVYIHIVGGGIFLQALINALATTIRTYGFTKQTMMVSLLMNLIHVAGNYLLIF 180 ++ AKV++ +VGG F+QALI + +++YG+TK TM V++ MN++++AGN+++IF Sbjct: 131 DALMPDAKVFLQVVGGLSFIQALIMTFSAILKSYGYTKDTMFVTIGMNILNIAGNFVVIF 190 Query: 181 GHFGLPALGVQGAAISTVASRLICLVIFFLLLYRIMEVRVKLAYYIHLSKKYVLQILKIG 240 G FG P LGV G A+ST +R+I L+ +++ + +++++ L H+ K+++ ++LKIG Sbjct: 191 GLFGFPVLGVAGVAMSTSIARVIGLIAMIVIVNKRIQLKMSLKKVFHMHKEHLRKLLKIG 250 Query: 241 IPSAFESVIYQSCQLIFTLYITYLGAEAMATRQYALNISNYIYLFSVAVAMGTSIIVGHL 300 IPSA E + Y Q+I T +I +GA+A+ T+ Y NI+ +I LF A++ GT I++G Sbjct: 251 IPSAGEQLSYNLSQMIVTYFIAIMGAQALTTKVYTQNITMFILLFGTAISQGTQILIGRY 310 Query: 301 VGARRPEEAYKRVFKSVKWALLVTVIIDVAVIFFRVPLMGLFTDNLEIIALGAQVILLSI 360 +G ++ + AY+R KS+ WAL + V + F L+GLFT + +IIA + +I ++I Sbjct: 311 IGGKQFDAAYERCMKSLYWALGIAAATSVLMTIFSKDLIGLFTQSPDIIATASLLIAMTI 370 Query: 361 FLETGRTCNLVIINSLRASGDAKFPVYMGFISMVCISLPLGYVLVFQLHLGLAGVWLATA 420 LE GR+ N++IINSLRA+GDAKFPVYM ISM I LPL Y+ L GLAG+W++ Sbjct: 371 ILEPGRSFNVIIINSLRAAGDAKFPVYMAMISMWGIGLPLAYLFGIHLGFGLAGIWISFI 430 Query: 421 ADEWLRAVIMFFRWKSRAWVKH 442 ADEW+R ++M+ RW+SR W++ Sbjct: 431 ADEWVRGILMYRRWRSRIWIQK 452 >BH2936 [V] COG0534 Na+-driven multidrug efflux pump Length = 452 Score = 323 bits (828), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 180/440 (40%), Positives = 273/440 (62%), Gaps = 1/440 (0%) Query: 1 MNQTKHQEFNLVKLTWPIFLELFLFMLMGSVDTFMIXXXXXXXXXXXXXXNQXXXXXXXX 60 M ++ L LTWPIF+E+ L MLMG+ DT M+ NQ Sbjct: 1 MKGETKKQLTLFALTWPIFIEILLHMLMGNADTLMLSQYSDDAVAAVGVSNQILAVIIVM 60 Query: 61 XXXXGNGAAIVVSQYLGSRKPKEAAEVTGNAVTLNLAVGIIFSTAILVFGEALLRALNVT 120 G +I+V+Q+LG+++ + A +V ++ NL GI+ ++ FG +L+A+ + Sbjct: 61 FGFVATGTSILVAQHLGAKERENAGKVAVVSIGANLIFGIVLGLLLIAFGPPILKAMQLD 120 Query: 121 GDILIHAKVYIHIVGGGIFLQALINALATTIRTYGFTKQTMMVSLLMNLIHVAGNYLLIF 180 +L A +Y+ IVGG +Q+LI +R++ FTK M V++ MN+++V GNYL IF Sbjct: 121 DSLLQEATLYLQIVGGFSVVQSLIMTAGAILRSHSFTKDVMYVTIGMNILNVIGNYLFIF 180 Query: 181 GHFGLPALGVQGAAISTVASRLICLVIFFLLLYRIMEVRVKLAYYIHLSKKYVLQ-ILKI 239 G FG+P LGV G A+STV SR I L + +LLY+ + + AY + + L+ +LKI Sbjct: 181 GPFGIPVLGVTGVALSTVVSRTIGLFVIAILLYKRIRGELPFAYLLKRFPRVELRNLLKI 240 Query: 240 GIPSAFESVIYQSCQLIFTLYITYLGAEAMATRQYALNISNYIYLFSVAVAMGTSIIVGH 299 GIPSA E + Y + QL+ T +I +G EA+ T+ Y N+ +++LF+VA+ GT I++GH Sbjct: 241 GIPSAGEQLSYNASQLVITYFIAMMGTEALTTKVYTQNLMMFVFLFAVAIGQGTQILIGH 300 Query: 300 LVGARRPEEAYKRVFKSVKWALLVTVIIDVAVIFFRVPLMGLFTDNLEIIALGAQVILLS 359 VGA++ + AY R F+S+ A+ V+V + V F PL+G+FTDN +I++LG ++LL+ Sbjct: 301 QVGAKQIQAAYVRCFRSLWIAMTVSVSMAVVFFAFSTPLLGIFTDNPDILSLGTTLLLLT 360 Query: 360 IFLETGRTCNLVIINSLRASGDAKFPVYMGFISMVCISLPLGYVLVFQLHLGLAGVWLAT 419 I LE GR CNLV+I+SLRA+GD KFPVY+ +SM I++P+ Y+L L LGL GVW+A Sbjct: 361 IILEPGRACNLVVISSLRAAGDVKFPVYLAIVSMWGIAVPIAYLLGLPLGLGLIGVWIAF 420 Query: 420 AADEWLRAVIMFFRWKSRAW 439 ADEW R ++M +RW+ W Sbjct: 421 IADEWFRGLLMIWRWRKGKW 440 >lin0614 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 273 bits (698), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 30/440 (6%), Positives = 74/440 (16%), Gaps = 1/440 (0%) Query: 1 MNQ-TKHQEFNLVKLTWPIFLELFLFMLMGSVDTFMIXXXXXXXXXXXXXXNQXXXXXXX 59 M++ + + + + + Sbjct: 1 MHRYVQKDIQGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTC 60 Query: 60 XXXXXGNGAAIVVSQYLGSRKPKEAAEVTGNAVTLNLAVGIIFSTAILVFGEALLRALNV 119 + V +R + V + + + I+V + + Sbjct: 61 YRLVWNHMTGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTL 120 Query: 120 TGDILIHAKVYIHIVGGGIFLQALINALATTIRTYGFTKQTMMVSLLMNLIHVAGNYLLI 179 + + L+ + A T + T + + + N Sbjct: 121 ANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGS 180 Query: 180 FGHFGLPALGVQGAAISTVASRLICLVIFFLLLYRIMEVRVKLAYYIHLSKKYVLQILKI 239 + A G Q V + L + + + + V + Sbjct: 181 VSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVV 240 Query: 240 GIPSAFESVIYQSCQLIFTLYITYLGAEAMATRQYALNISNYIYLFSVAVAMGTSIIVGH 299 + F RQ A T Sbjct: 241 NTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHAL 300 Query: 300 LVGARRPEEAYKRVFKSVKWALLVTVIIDVAVIFFRVPLMGLFTDNLEIIALGAQVILLS 359 + + + V L++ A G + Sbjct: 301 DTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVTL 360 Query: 360 IFLETGRTCNLVIINSLRASGDAKFPVYMGFISMVCISLPLGYVLVFQLHLGLAGVWLAT 419 T + + G ++ L + Sbjct: 361 KQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTL 420 Query: 420 AADEWLRAVIMFFRWKSRAW 439 A + Sbjct: 421 DVRNGGTATTVSMGNGGVLL 440 >FN0162 [V] COG0534 Na+-driven multidrug efflux pump Length = 446 Score = 268 bits (685), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 146/435 (33%), Positives = 238/435 (54%) Query: 11 LVKLTWPIFLELFLFMLMGSVDTFMIXXXXXXXXXXXXXXNQXXXXXXXXXXXXGNGAAI 70 L+ LT PIFLEL L ++G++DT M+ Q +I Sbjct: 12 LISLTIPIFLELLLVTVVGNIDTIMLGYYSDEAVGAIGGITQLLNIQNVIFSFINMATSI 71 Query: 71 VVSQYLGSRKPKEAAEVTGNAVTLNLAVGIIFSTAILVFGEALLRALNVTGDILIHAKVY 130 + +Q+LG++ K +V ++ LN+ +G++ L F LL+ +N+ +++ K Y Sbjct: 72 LTAQFLGAKDYKRVKQVISVSLVLNILLGVVLGGIYLFFWRGLLQKMNLPEELVGIGKYY 131 Query: 131 IHIVGGGIFLQALINALATTIRTYGFTKQTMMVSLLMNLIHVAGNYLLIFGHFGLPALGV 190 +VGG Q +I + ++++G +T+++++ +N++++ GN L IFG G+P LG Sbjct: 132 FQLVGGLCIFQGIILSCGAILKSHGRATETLIINVGVNILNILGNALFIFGWLGMPVLGP 191 Query: 191 QGAAISTVASRLICLVIFFLLLYRIMEVRVKLAYYIHLSKKYVLQILKIGIPSAFESVIY 250 G ISTV SR I V+ F ++ + + Y K V IL IG+P+A E++ + Sbjct: 192 TGVGISTVISRGIGCVVAFYMMCKYCNFTFRKKYIKPFPFKIVKNILSIGLPTAGENLSW 251 Query: 251 QSCQLIFTLYITYLGAEAMATRQYALNISNYIYLFSVAVAMGTSIIVGHLVGARRPEEAY 310 QL+ + +G +A+R Y + IS++I S+A+ GT+I +GHLVGA +E Y Sbjct: 252 NMGQLMIVAMVNTMGTTIIASRTYLMLISSFIMTLSIALGQGTAIQIGHLVGAGEIKEVY 311 Query: 311 KRVFKSVKWALLVTVIIDVAVIFFRVPLMGLFTDNLEIIALGAQVILLSIFLETGRTCNL 370 + KSVK A + + V R P+M +FT N +I+ + ++ L I LE GR N+ Sbjct: 312 NKCLKSVKIAFIFAFVATSIVCLLRKPIMNIFTTNPDILKVSLKIFPLMIILEMGRVFNI 371 Query: 371 VIINSLRASGDAKFPVYMGFISMVCISLPLGYVLVFQLHLGLAGVWLATAADEWLRAVIM 430 VIINSL A+GD KFP++MG I + I++ Y+ L GL G+W+A A DEW+R + M Sbjct: 372 VIINSLHAAGDIKFPMFMGIIFVFTIAVLFSYIFGISLGWGLVGIWIANAMDEWIRGIAM 431 Query: 431 FFRWKSRAWVKHRLI 445 +FRWKS+ W + Sbjct: 432 YFRWKSKKWQNKSFV 446 >Cj0619 [V] COG0534 Na+-driven multidrug efflux pump Length = 438 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 137/446 (30%), Positives = 236/446 (52%), Gaps = 18/446 (4%) Query: 7 QEFNLVKLTWPIFLELFLFMLMGSVDTFMIXXXXXXXXXXXXXXNQXXXXXXXXXXXXGN 66 ++ +L +L++PIF +L L ++T M+ NQ Sbjct: 4 KQLSLTRLSFPIFWDLLSKYLTVIINTAMVSHYSNFLVGAMGAGNQILDLFITIFSFLSV 63 Query: 67 GAAIVVSQYLGSRKPKEAAEVTGNAVTLNLAVGIIFSTAILVFGEALLRALNVTGDILIH 126 G +++++Q +G++ A +V ++ LN +G + IL GE LL L + ++L Sbjct: 64 GCSVLIAQAIGAKDHVLARKVIHQSLFLNALLGFVCGVLILWHGEYLLYLLKIPQELLKD 123 Query: 127 AKVYIHIVGGGIFLQALINALATTIRTYGFTKQTMMVSLLMNLIHVAGNYLLIFGHFGLP 186 +++Y+H++ +F A+ LA +R Y M + LM+++ + GNY ++ H+ Sbjct: 124 SEIYLHMLAICLFFDAIGIVLAAIVRVYNMAYWVMFIGFLMDIVVICGNYYVL--HYTKS 181 Query: 187 ALGVQGAAISTVASRLICLVIFFLLLYRIMEVRVKLAYYIHLSKKYVLQILKIGIPSAFE 246 L G +S + +R++ +V F +L+ +++ +K+ I L K+ + ++L IG SA E Sbjct: 182 EL--FGVGLSNIFARIVAIVALFAILFYKLKIHLKIKEMIKLEKEVLKKVLNIGGFSAGE 239 Query: 247 SVIYQSCQLIFTLYITYLGAEAMATRQYALNISNYIYLFSVAVAMGTSIIVGHLVGARRP 306 ++++ I ++ LG +++ + IS I L A+++ IIVG L+GAR Sbjct: 240 NLLWIVQYTIAFAFVASLGEASLSVQTIYFQISMLIMLIGQAISIANEIIVGKLIGARYE 299 Query: 307 EEAYKRVFKSVKWALLVTVIIDVAVIF-------FRVPLMGLFTDNLEIIALGAQVILLS 359 AYK ++ AL +VI V F F + ++GL E+ L + LS Sbjct: 300 NIAYKHAWR----ALYFSVIASALVAFLNYLCQDFTMQILGL---KEELKNLMIPLFALS 352 Query: 360 IFLETGRTCNLVIINSLRASGDAKFPVYMGFISMVCISLPLGYVLVFQLHLGLAGVWLAT 419 IFLE RT N+V++NSLRASGDAKFP + G + M+ +SLP+GYVL F +LG+ GVW+ Sbjct: 353 IFLEISRTFNIVMVNSLRASGDAKFPFFSGLVFMMGVSLPVGYVLCFHFNLGILGVWIGF 412 Query: 420 AADEWLRAVIMFFRWKSRAWVKHRLI 445 ADE+LR ++ +RWKS+ W L+ Sbjct: 413 CADEFLRGMVNSYRWKSKKWQGKALV 438 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.143 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,114,528 Number of extensions: 1071186 Number of successful extensions: 4632 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 4447 Number of HSP's successfully gapped: 115 Length of query: 461 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 351 Effective length of database: 25,691,691 Effective search space: 9017783541 Effective search space used: 9017783541 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 118 (50.1 bits)