BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0011.p (410 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pepT [E] COG2195 Di- and tripeptidases 594 e-170 lin1892 [E] COG2195 Di- and tripeptidases 561 e-160 FN0733 [E] COG2195 Di- and tripeptidases 461 e-130 SA0698 [E] COG2195 Di- and tripeptidases 423 e-118 pepT [E] COG2195 Di- and tripeptidases 417 e-116 >BS_pepT [E] COG2195 Di- and tripeptidases Length = 408 Score = 594 bits (1532), Expect = e-170, Method: Compositional matrix adjust. Identities = 0/410 (0%), Positives = 0/410 (0%) Query: 1 MKEKLIERFISYAQMDTQSNDDNEACPSTPGQMELARKLAAELQELGLVEVTVDEHAYVM 60 M + L + + + L + + ++ + Sbjct: 1 MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEEMGLINVTLSEKGTLMA 60 Query: 61 ATLPANTDKEVPVIGFLAHLDTATDFTGAGVKPQLVENYDGKDIVLNKELDIVLSTESFP 120 + + + G + + + Sbjct: 61 TLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGDIALGIGDEVLSPVMFPVL 120 Query: 121 ELAGYKXXXXXXXXXXXXXXADNKAGIAEIMTAMAHLLEHPEIKHGKIRVAFTPDEEIGR 180 + A+ + P + + Sbjct: 121 HQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKKIPHGDIRVAFTPDEEVGKGAK 180 Query: 181 GPHKFDVARFGASHAYTVDGGPLGELEYESFNAAGVKISFKGVNVHPGTAKGKMIHSAKI 240 A G L + + + + + A Sbjct: 181 HFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIVGNNVHPGTAKGVMVNALSLAAR 240 Query: 241 AMAFHLRLPSGEAPEFTEGYEGFYHLISMQGTAEQSKLQYIIRDFDREAFENRKAYIAAI 300 A S E E EG+ + A+ + EA + + IA Sbjct: 241 IHAEVPADESPEMTEGYEGFYHLASMKGTVERADMHYIIRDFDRKQFEARKRKMMEIAKK 300 Query: 301 VEEFQSTYGAENIVLEMKDQYYNMREKIEPVRHIVDIAQEAMENLGITPVIRPIRGGTDG 360 V + +V+E + P + ++ Sbjct: 301 VGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGAQ 360 Query: 361 SQLSYMGLPTPNIFTGGENFHGKFEYASVDVMLKAVQVIVEIAKLTEQKA 410 Sbjct: 361 410 >lin1892 [E] COG2195 Di- and tripeptidases Length = 410 Score = 561 bits (1447), Expect = e-160, Method: Compositional matrix adjust. Identities = 267/409 (65%), Positives = 320/409 (78%) Query: 1 MKEKLIERFISYAQMDTQSNDDNEACPSTPGQMELARKLAAELQELGLVEVTVDEHAYVM 60 MKE+L++RF Y ++DTQSN+++ CP+TPGQMELA L EL+E+G+ EVTVDE YVM Sbjct: 1 MKEELLKRFTKYVKVDTQSNEESTVCPTTPGQMELANILVTELKEIGMQEVTVDEFGYVM 60 Query: 61 ATLPANTDKEVPVIGFLAHLDTATDFTGAGVKPQLVENYDGKDIVLNKELDIVLSTESFP 120 ATLP+NT KEVPVIGFLAHLDTATD TG V PQ+ ENYDGKDIVLNK+L++VLS + FP Sbjct: 61 ATLPSNTTKEVPVIGFLAHLDTATDLTGKNVNPQVHENYDGKDIVLNKDLNVVLSPKQFP 120 Query: 121 ELAGYKXXXXXXXXXXXXXXADNKAGIAEIMTAMAHLLEHPEIKHGKIRVAFTPDEEIGR 180 ELA YK AD+KAGI EIM AM +L+ HPEIKHGKIRVAFTPDEEIGR Sbjct: 121 ELANYKGKTLITTDGTTLLGADDKAGITEIMVAMNYLINHPEIKHGKIRVAFTPDEEIGR 180 Query: 181 GPHKFDVARFGASHAYTVDGGPLGELEYESFNAAGVKISFKGVNVHPGTAKGKMIHSAKI 240 GP +FDV FGA +AYT+DGGPLGELEYESFNAAG K++F G +VHPGTAK KMI++ K+ Sbjct: 181 GPERFDVEAFGAKYAYTMDGGPLGELEYESFNAAGAKLTFNGNSVHPGTAKNKMINAVKM 240 Query: 241 AMAFHLRLPSGEAPEFTEGYEGFYHLISMQGTAEQSKLQYIIRDFDREAFENRKAYIAAI 300 AM F +P EAPE+T+GYEGFYHLIS+ G EQ+K YIIRDFD F RK +IA+I Sbjct: 241 AMEFDAEIPKEEAPEYTDGYEGFYHLISLNGDVEQAKSYYIIRDFDHLKFVERKTHIASI 300 Query: 301 VEEFQSTYGAENIVLEMKDQYYNMREKIEPVRHIVDIAQEAMENLGITPVIRPIRGGTDG 360 E+ YG ++ L++ DQYYNMREKIEPV+ IVDI AM NL I P I PIRGGTDG Sbjct: 301 AEKLAEKYGEGSVELKLNDQYYNMREKIEPVKEIVDIVSAAMRNLDIEPKISPIRGGTDG 360 Query: 361 SQLSYMGLPTPNIFTGGENFHGKFEYASVDVMLKAVQVIVEIAKLTEQK 409 +QLSY GLPTPNIF GGENFHGKFEY +++ M+KA +VI+E+A+L E+K Sbjct: 361 AQLSYKGLPTPNIFGGGENFHGKFEYVALESMVKATEVIIEVARLFEEK 409 >FN0733 [E] COG2195 Di- and tripeptidases Length = 412 Score = 461 bits (1187), Expect = e-130, Method: Compositional matrix adjust. Identities = 223/405 (55%), Positives = 288/405 (71%), Gaps = 1/405 (0%) Query: 5 LIERFISYAQMDTQSNDDNEACPSTPGQMELARKLAAELQELGLVEVTVDEHAYVMATLP 64 L ERF+ Y + +T+S+D +E PSTP QME A+ L EL+ELGL + +++ +V ATLP Sbjct: 9 LKERFLRYVKFNTRSDDASETIPSTPSQMEFAKMLKKELEELGLSNIFINKACFVNATLP 68 Query: 65 ANTDKEVPVIGFLAHLDTATDFTGAGVKPQLVENYDGKDIVLNKELDIVLSTESFPELAG 124 +N DK+V +GF+AH+DTA DF G+ PQ+VENYDGKDIVLNKE +IVL E FP L Sbjct: 69 SNIDKKVATVGFIAHMDTA-DFNAEGISPQIVENYDGKDIVLNKEQNIVLKVEEFPNLKN 127 Query: 125 YKXXXXXXXXXXXXXXADNKAGIAEIMTAMAHLLEHPEIKHGKIRVAFTPDEEIGRGPHK 184 Y AD+K+GI EI+ A+ +L EHPEIKHG I++AF PDEEIGRG Sbjct: 128 YISKTLITTDGTTLLGADDKSGIVEIIEAVKYLKEHPEIKHGDIKIAFGPDEEIGRGADY 187 Query: 185 FDVARFGASHAYTVDGGPLGELEYESFNAAGVKISFKGVNVHPGTAKGKMIHSAKIAMAF 244 FDV F A +AYT+DGGP+GELEYESFNAA K KGV+VHPGTAKGKMI+++ IA Sbjct: 188 FDVKEFAADYAYTMDGGPIGELEYESFNAAQAKFKIKGVSVHPGTAKGKMINASLIASEI 247 Query: 245 HLRLPSGEAPEFTEGYEGFYHLISMQGTAEQSKLQYIIRDFDREAFENRKAYIAAIVEEF 304 P E PE TEGYEGFY L M+ E+ ++ YIIRD D+ F +K ++ +VE+ Sbjct: 248 IEMFPKDEVPEKTEGYEGFYFLDEMKSNCEEGEVVYIIRDHDKAKFLAKKEFVKELVEKV 307 Query: 305 QSTYGAENIVLEMKDQYYNMREKIEPVRHIVDIAQEAMENLGITPVIRPIRGGTDGSQLS 364 YG E + LE+KD+YYNM E I+ ++VDIA++AMENLGI P+I+ IRGGTDGS++S Sbjct: 308 NKKYGREVVKLELKDEYYNMGEIIKDHMYVVDIAKQAMENLGIKPLIKAIRGGTDGSKIS 367 Query: 365 YMGLPTPNIFTGGENFHGKFEYASVDVMLKAVQVIVEIAKLTEQK 409 +MGLPTPNIF GGENFHGK+E+ +++ M KA VIVEI KL ++ Sbjct: 368 FMGLPTPNIFAGGENFHGKYEFVALESMEKATDVIVEIVKLNAER 412 >SA0698 [E] COG2195 Di- and tripeptidases Length = 408 Score = 423 bits (1088), Expect = e-118, Method: Compositional matrix adjust. Identities = 213/404 (52%), Positives = 269/404 (66%), Gaps = 2/404 (0%) Query: 1 MKEKLIERFISYAQMDTQSNDDNEACPSTPGQMELARKLAAELQELGLVEVTVDEHAYVM 60 MK +LI+R Y +DTQS+ + PST Q +L L ELQ+LGL +DE+ Y+ Sbjct: 1 MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQQLGL-PTDLDENGYLF 59 Query: 61 ATLPANTDKEVPVIGFLAHLDTATDFTGAGVKPQLVENYDGKDIVLNKELDIVLSTESFP 120 ATL +N D +VP +GFLAH+DT+ DF + VKPQ++ENYDGK L VL + FP Sbjct: 60 ATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKPYKLGN-TKRVLDPKVFP 118 Query: 121 ELAGYKXXXXXXXXXXXXXXADNKAGIAEIMTAMAHLLEHPEIKHGKIRVAFTPDEEIGR 180 EL AD+KAGI EIM A+ +L EHPEIKHG IR+ FTPDEEIGR Sbjct: 119 ELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPEIKHGTIRIGFTPDEEIGR 178 Query: 181 GPHKFDVARFGASHAYTVDGGPLGELEYESFNAAGVKISFKGVNVHPGTAKGKMIHSAKI 240 GPHKFDV RF A AYT+DG GEL+YESFNAA I+ GVNVHPG+AK M+++ ++ Sbjct: 179 GPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITCHGVNVHPGSAKNAMVNAIRL 238 Query: 241 AMAFHLRLPSGEAPEFTEGYEGFYHLISMQGTAEQSKLQYIIRDFDREAFENRKAYIAAI 300 F LP E PE TEGYEGFYHL++ +GT E++ LQYIIRD D++ FE RK I I Sbjct: 239 GEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATLQYIIRDHDKKQFELRKKRILEI 298 Query: 301 VEEFQSTYGAENIVLEMKDQYYNMREKIEPVRHIVDIAQEAMENLGITPVIRPIRGGTDG 360 ++ + + + +++ DQY+NM EKI P+ HI+DI + L I PIRGGTDG Sbjct: 299 RDDINAHFENYPVKVDISDQYFNMAEKILPLPHIIDIPKRVFAKLDIPANTEPIRGGTDG 358 Query: 361 SQLSYMGLPTPNIFTGGENFHGKFEYASVDVMLKAVQVIVEIAK 404 SQLS+MGLPTPNIFTG NFHG +EYAS+DVM KAVQVI+ I + Sbjct: 359 SQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEKAVQVIIGIVE 402 >pepT [E] COG2195 Di- and tripeptidases Length = 408 Score = 417 bits (1072), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/408 (52%), Positives = 279/408 (68%), Gaps = 3/408 (0%) Query: 3 EKLIERFISYAQMDTQSNDDNEACPSTPGQMELARKLAAELQELGLVEVTVDEHAYVMAT 62 +KL+ERF++Y +DTQS PST GQ +L L +L+E+GL+ VT+ E +MAT Sbjct: 2 DKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEEMGLINVTLSEKGTLMAT 61 Query: 63 LPANTDKEVPVIGFLAHLDTATDFTGAGVKPQLVENYDGKDIVLNKELDIVLSTESFPEL 122 LPAN ++P IGF++H+DT+ D +G V PQ+VENY G DI L D VLS FP L Sbjct: 62 LPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGDIALGIG-DEVLSPVMFPVL 120 Query: 123 AGYKXXXXXXXXXXXXXXADNKAGIAEIMTAMAHLLEHPEIKHGKIRVAFTPDEEIGRGP 182 AD+KAGIAEIMTA+A +L+ +I HG IRVAFTPDEE+G+G Sbjct: 121 HQLLGQTLITTDGKTLLGADDKAGIAEIMTALA-VLQQKKIPHGDIRVAFTPDEEVGKGA 179 Query: 183 HKFDVARFGASHAYTVDGGPLGELEYESFNAAGVKISFKGVNVHPGTAKGKMIHSAKIAM 242 FDV F A AYTVDGG +GELE+E+FNAA V I G NVHPGTAKG M+++ +A Sbjct: 180 KHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIVGNNVHPGTAKGVMVNALSLAA 239 Query: 243 AFHLRLPSGEAPEFTEGYEGFYHLISMQGTAEQSKLQYIIRDFDREAFENRKAYIAAIVE 302 H +P+ E+PE TEGYEGFYHL SM+GT E++ + YIIRDFDR+ FE RK + I + Sbjct: 240 RIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMHYIIRDFDRKQFEARKRKMMEIAK 299 Query: 303 EF-QSTYGAENIVLEMKDQYYNMREKIEPVRHIVDIAQEAMENLGITPVIRPIRGGTDGS 361 + + + I L ++D YYNMREK+ HI+DIAQ+AM + I P ++PIRGGTDG+ Sbjct: 300 KVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGA 359 Query: 362 QLSYMGLPTPNIFTGGENFHGKFEYASVDVMLKAVQVIVEIAKLTEQK 409 QLS+MGLP PN+FTGG N+HGK E+ +++ M KAVQVIV IA+LT Q+ Sbjct: 360 QLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKAVQVIVRIAELTAQR 407 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,896,462 Number of extensions: 1394013 Number of successful extensions: 6382 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 6344 Number of HSP's successfully gapped: 19 Length of query: 410 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 301 Effective length of database: 25,807,025 Effective search space: 7767914525 Effective search space used: 7767914525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)