BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0043.0007.p (337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvbT [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 423 e-118 ZyhbW [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetra... 381 e-105 ECs4041 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 381 e-105 YPO3484 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 380 e-105 ZyhbW [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetra... 379 e-105 >BS_yvbT [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 423 bits (1088), Expect = e-118, Method: Compositional matrix adjust. Identities = 28/332 (8%), Positives = 62/332 (18%), Gaps = 3/332 (0%) Query: 5 QDIPFSVLDLAPIVEGGTAADSFHNTLDLARHAEAWGYHRYWLAEHHNMPGIASSATSLV 64 V L +S + +A A G + +AE + +P + Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 65 IGHVAAGTKSIRVGSGGIMLSNHAPLMIAEQFGTLESLYPGRIDLGLGRAPGSDQAAARA 124 K I P ++ + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 125 MRRGLGQDGSEFPEQLSELRAYFDPDGAGSRPLGVRAVPGEGLNVPIWLLGSSGFSAQLA 184 + +L + + + + G S + A Sbjct: 127 DLYQV-HWPQRPTNCFGKLGYSWTDSAPAVSL--LDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 185 GQLGLPFAFASHFAPDYLLPALHLYRTSFKPSAVLDKPHVMVGLGITAAETTEQARWLAT 244 + A ++ + Y + V A + L Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 245 SQQQQFLNIIRGRTGKLQPPVDSMEGLWSPQEKAMLLSKQLYSIAGDQAAIKERLLQIVE 304 T + S E + ++++ A R V Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 305 ETQADEFIVASQIYDHSARLHSYELVADLVKG 336 T + + + Sbjct: 304 STLLGATTMDQLKTNIESLHLELSEDVLAEIE 335 >ZyhbW [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 381 bits (978), Expect = e-105, Method: Compositional matrix adjust. Identities = 22/329 (6%), Positives = 62/329 (18%), Gaps = 3/329 (0%) Query: 5 QDIPFSVLDLAPIVEGGTAADSFHNTLDLARHAEAWGYHRYWLAEHHNMPGIASSATSLV 64 IP S L+++ + G ++ D + L + M + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 65 IGHVAAGTKSIRVGSGGIMLSNHAPLMIAEQFGTLESLYPGRIDLGLGRAPGSDQAAARA 124 + G + GS ++ + + A + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 125 MRRGLGQDGSEFPEQLSELRAYFDPDGAGSRPLGVRAVPGEGLNVPIWLLGSSGFSAQLA 184 L Q + + + V + G + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA---VSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 185 GQLGLPFAFASHFAPDYLLPALHLYRTSFKPSAVLDKPHVMVGLGITAAETTEQARWLAT 244 + L + + + + V T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 245 SQQQQFLNIIRGRTGKLQPPVDSMEGLWSPQEKAMLLSKQLYSIAGDQAAIKERLLQIVE 304 + + Q+ D A + ++ Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 305 ETQADEFIVASQIYDHSARLHSYELVADL 333 + + + + +++ Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSED 329 >ECs4041 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 381 bits (978), Expect = e-105, Method: Compositional matrix adjust. Identities = 22/329 (6%), Positives = 62/329 (18%), Gaps = 3/329 (0%) Query: 5 QDIPFSVLDLAPIVEGGTAADSFHNTLDLARHAEAWGYHRYWLAEHHNMPGIASSATSLV 64 IP S L+++ + G ++ D + L + M + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 65 IGHVAAGTKSIRVGSGGIMLSNHAPLMIAEQFGTLESLYPGRIDLGLGRAPGSDQAAARA 124 + G + GS ++ + + A + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 125 MRRGLGQDGSEFPEQLSELRAYFDPDGAGSRPLGVRAVPGEGLNVPIWLLGSSGFSAQLA 184 L Q + + + V + G + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA---VSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 185 GQLGLPFAFASHFAPDYLLPALHLYRTSFKPSAVLDKPHVMVGLGITAAETTEQARWLAT 244 + L + + + + V T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 245 SQQQQFLNIIRGRTGKLQPPVDSMEGLWSPQEKAMLLSKQLYSIAGDQAAIKERLLQIVE 304 + + Q+ D A + ++ Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 305 ETQADEFIVASQIYDHSARLHSYELVADL 333 + + + + +++ Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSED 329 >YPO3484 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Length = 346 Score = 380 bits (975), Expect = e-105, Method: Compositional matrix adjust. Identities = 18/327 (5%), Positives = 43/327 (13%), Gaps = 3/327 (0%) Query: 7 IPFSVLDLAPIVEGGTAADSFHNTLDLARHAEAWGYHRYWLAEHHNMPGIASSATSLVIG 66 + A + + A Y E + G Sbjct: 18 LGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHG 77 Query: 67 HVAAGTKSIRVGSGGIMLSNHAPLMIAEQFGTLESLYPGRIDLGLGRAPGSDQAAARAMR 126 + +V A + + Q Sbjct: 78 SREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRP 137 Query: 127 RGLGQDGSEFPEQLSELRAYFDPDGAGSRPLGVRAVPGEGLNVPIWLLGSSGFSAQLAGQ 186 + + D A + + Sbjct: 138 TNCFGKLGYSWTDSAPAVSLLDTLDALA---EYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 187 LGLPFAFASHFAPDYLLPALHLYRTSFKPSAVLDKPHVMVGLGITAAETTEQARWLATSQ 246 + P LL + + LG Sbjct: 195 KHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGA 254 Query: 247 QQQFLNIIRGRTGKLQPPVDSMEGLWSPQEKAMLLSKQLYSIAGDQAAIKERLLQIVEET 306 + + +G+ + + + L + L + Sbjct: 255 RNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQ 314 Query: 307 QADEFIVASQIYDHSARLHSYELVADL 333 + Sbjct: 315 LKTNIESLHLELSEDVLAEIEAVHQVY 341 Score = 379 bits (973), Expect = e-105, Method: Compositional matrix adjust. Identities = 22/329 (6%), Positives = 62/329 (18%), Gaps = 3/329 (0%) Query: 5 QDIPFSVLDLAPIVEGGTAADSFHNTLDLARHAEAWGYHRYWLAEHHNMPGIASSATSLV 64 IP S L+++ + G ++ D + L + M + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 65 IGHVAAGTKSIRVGSGGIMLSNHAPLMIAEQFGTLESLYPGRIDLGLGRAPGSDQAAARA 124 + G + GS ++ + + A + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 125 MRRGLGQDGSEFPEQLSELRAYFDPDGAGSRPLGVRAVPGEGLNVPIWLLGSSGFSAQLA 184 L Q + + + V + G + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA---VSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 185 GQLGLPFAFASHFAPDYLLPALHLYRTSFKPSAVLDKPHVMVGLGITAAETTEQARWLAT 244 + L + + + + V T Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 245 SQQQQFLNIIRGRTGKLQPPVDSMEGLWSPQEKAMLLSKQLYSIAGDQAAIKERLLQIVE 304 + + Q+ D A + ++ Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 305 ETQADEFIVASQIYDHSARLHSYELVADL 333 + + + + +++ Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSED 329 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,018,826 Number of extensions: 1144722 Number of successful extensions: 3418 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 3397 Number of HSP's successfully gapped: 27 Length of query: 337 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 230 Effective length of database: 26,037,693 Effective search space: 5988669390 Effective search space used: 5988669390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)