BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0010.p (330 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0754 [T] COG0642 Signal transduction histidine kinase 184 2e-46 CAC0225 [T] COG0642 Signal transduction histidine kinase 167 3e-41 CAC0372 [T] COG0642 Signal transduction histidine kinase 151 1e-36 lin1852 [T] COG0642 Signal transduction histidine kinase 150 3e-36 SP1632 [T] COG0642 Signal transduction histidine kinase 138 1e-32 >BH0754 [T] COG0642 Signal transduction histidine kinase Length = 353 Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 100/245 (40%), Positives = 148/245 (60%), Gaps = 11/245 (4%) Query: 89 AVSELLQATHRHYQEQLQSHISRMDQHIIFINRWVHQMKTPLSVIQLTLQELDDEA---- 144 A+ +L Y +L + + + F NRW HQMKTPLSVI+L LQE D+ + Sbjct: 98 AMIKLFGQAFEDYSTRLHTAQEQHSRSYHFANRWTHQMKTPLSVIRLILQEQDEWSKEVL 157 Query: 145 AGSIQEELERLRKGLEMVIYTSRLERFEDDFQVQSLPLRRIISEAVAENRRLFIRRGIKV 204 I+EE+ +L GL M++ T RL++FE DF ++ + L +I + R+ FIR I Sbjct: 158 VDQIEEEVGKLEDGLHMMLSTVRLQKFELDFSIERIALLSVIRSVLHGKRKTFIRYRIYP 217 Query: 205 DIRVDESLTVY--SDAKWLMFMLTQILVNAVNYTSG----SGKTVAVTAQAMGKDIQLDI 258 + + E+ V+ +D KWL+F+L Q++ NA+ Y+S K + V + + + I Sbjct: 218 RL-ISEAEEVFAATDKKWLIFVLEQLIQNALKYSSTLAREPSKYITVRVEETDNGVTVSI 276 Query: 259 ADQGIGISSEDLKRVFNPYFTGERGRQYHESTGMGLYLVREICSRLGHKVGLESKPGEGT 318 D+GIGI +EDL RVF+P+FTGE GR+ ESTGMGLYLV++IC +LGH + +ES EGT Sbjct: 277 QDEGIGIVAEDLPRVFDPFFTGENGRKLQESTGMGLYLVKQICDQLGHSITIESTVNEGT 336 Query: 319 TVRII 323 TV + Sbjct: 337 TVTLF 341 >CAC0225 [T] COG0642 Signal transduction histidine kinase Length = 339 Score = 167 bits (422), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 97/329 (29%), Positives = 173/329 (52%), Gaps = 15/329 (4%) Query: 4 FLKDQRPLLLFYLLQMILVPVLYYLSGESRPLSIILYGMXXXXXXXXXXXXXXXIQHHKL 63 +LKD +L Y+L V YL ++R LS + + + Sbjct: 6 YLKDNFRFILLYVLLGAFVSAFTYLDEKNRMLS---SNIGYMIFVMFFILLIFLCVDYSI 62 Query: 64 YSRLAQPAAAPGELS--VPLDDAPL---SEAVSELLQATHRHYQEQLQSHISRMDQHIIF 118 ++ + A GE+ P+ PL E +++ ++ Y E++ S + + F Sbjct: 63 KNKHIKKLIANGEIKDKTPILPEPLEYKDEVYISIIEDLYKDYNERIVSLEKEFEDNNEF 122 Query: 119 INRWVHQMKTPLSVIQLTLQ--ELDDEAAGSIQEELERLRKGLEMVIYTSRLERFEDDFQ 176 + W+H++KTP++ +L L+ E+D + EE+E++ +E V+Y SR + F D+ Sbjct: 123 MTAWIHEIKTPIATAKLLLESGEIDSQL---FMEEIEKIDDYVEKVLYYSRSDNFSKDYI 179 Query: 177 VQSLPLRRIISEAVAENRRLFIRRGIKVDIRVDESLTVYSDAKWLMFMLTQILVNAVNYT 236 + + + ++I E + + ++FI++ IK+ + +DE V +D KW +F+L QI+ NA+ YT Sbjct: 180 ISEVNISKLIKECIKSHSKIFIKKHIKLKLEIDERFNVETDKKWFLFILNQIISNALKYT 239 Query: 237 SGSGKTVAVTAQAMGKDIQLDIADQGIGISSEDLKRVFNPYFTGERGRQYH-ESTGMGLY 295 + G + + + + + I D GIGI EDL R+F+ FTG GR+ + ++TGMGLY Sbjct: 240 NDMGNIIIKAFEDDNEKVVI-IEDNGIGIKKEDLDRIFSKSFTGYNGRKENSKATGMGLY 298 Query: 296 LVREICSRLGHKVGLESKPGEGTTVRIIF 324 L ++ +LGH + +ESK EGT + I F Sbjct: 299 LSNKLAEKLGHNITIESKYNEGTKLYIHF 327 >CAC0372 [T] COG0642 Signal transduction histidine kinase Length = 334 Score = 151 bits (382), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 82/249 (32%), Positives = 139/249 (55%), Gaps = 10/249 (4%) Query: 84 APLSEAVSELLQATHRHYQEQLQSHISRMDQHI-IFINRWVHQMKTPLSVIQLTLQELDD 142 P E + H++Y ++ S I + I FI++W+H MKTP+SVI L +Q + Sbjct: 84 TPEQRMAQEAILKIHKNYAAKI-SEIDYKNSDIKYFISQWIHNMKTPVSVIDLIVQREKE 142 Query: 143 EAAG----SIQEELERLRKGLEMVIYTSRLERFEDDFQVQSLPLRRIISEAVAENRRLFI 198 + +G +I+EE +++ G++ V+ RL+ F D++ + + + ++ + + FI Sbjct: 143 KLSGDVIKNIEEENYKIKNGMDQVLNIIRLDEFSRDYEPEIVDIVELVKRTINLKKNQFI 202 Query: 199 RRGI--KVDIRVDESLTVYSDAKWLMFMLTQILVNAVNYTSGSGK-TVAVTAQAMGKDIQ 255 KV+ VD++L V +D KW F++ QI+ N++ Y+ K V Sbjct: 203 YGNAFPKVEFHVDKAL-VLTDKKWSTFIIDQIISNSIKYSKKEEKGYVYFNIVQSNNKTH 261 Query: 256 LDIADQGIGISSEDLKRVFNPYFTGERGRQYHESTGMGLYLVREICSRLGHKVGLESKPG 315 L I D G+GI DLKR+F P+FTGE GR + STG+GLY+ ++I LGH++ +ES+ Sbjct: 262 LVIKDNGVGIPKYDLKRIFEPFFTGENGRNFENSTGIGLYICKKIADNLGHEISVESEIN 321 Query: 316 EGTTVRIIF 324 +GT V+I + Sbjct: 322 KGTIVKITY 330 >lin1852 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 0/332 (0%), Positives = 0/332 (0%), Gaps = 14/332 (4%) Query: 4 FLKDQRPLLLFYLLQMILVPVLYYLSGESR-PLSIILYGMXXXXXXXXXXXXXXXIQHHK 62 + + L+ + G + + Sbjct: 6 VILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNS 65 Query: 63 LYSRLAQPAAAPGELSV-PLDDAPLSEAVSELLQATHRHYQEQLQSHISRMD----QHII 117 + + + + + E + R+ I + Sbjct: 66 VCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSG-IVENEVCPVFAAR 124 Query: 118 FINRWVHQMKTPLSVIQLTLQ--ELDDEAA--GSIQEELERLRKGLEMVIYTSRLERFED 173 + + L +A Sbjct: 125 TTSALQINDDEVMDYQWCDLADVLHGIDATPW 184 Query: 174 DFQVQSLPLRRIISEAVAENRRLFIRRGIKVDIRVDESLTVYSDAKWLMFMLTQILVNAV 233 Sbjct: 185 - 243 Query: 234 NYTSGSGKT-VAVTAQAMGKDIQLDIADQGIGISSEDLKRVFNPYFTGERGRQYHESTGM 292 Sbjct: 244 - 302 Query: 293 GLYLVREICSRLGHKVGLESKPGEGTTVRIIF 324 Sbjct: 303 334 >SP1632 [T] COG0642 Signal transduction histidine kinase Length = 324 Score = 138 bits (348), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 79/247 (31%), Positives = 139/247 (56%), Gaps = 8/247 (3%) Query: 84 APLSEAVSELLQATHRHYQEQLQSHISRMDQHIIFINRWVHQMKTPLSVIQLTLQEL-DD 142 +PL A++E L+A +Q ++ + + WVHQ+KTP++ QL + E+ D Sbjct: 82 SPLEIALAEKLEAREMELYQQRSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDR 141 Query: 143 EAAGSIQEELERLRKGLEMVIYTSRLERFEDDFQVQSLPLRRIISEAVAENRRLFIRRGI 202 + +++E+ ++ +V+ RLE F DD ++ + + ++ E + + FI++G+ Sbjct: 142 QLKQQLEQEIFKIDSYTNLVLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGL 201 Query: 203 KVDIRVDESLTVYSDAKWLMFMLTQILVNAVNYTSGSGKTVAVTAQAMGKDIQLDIADQG 262 V++ D + +D KWL+ ++ QI+ N++ YT G + + Q +L I D G Sbjct: 202 NVNLH-DLDKEIVTDKKWLLVVIEQIISNSLKYTKEGGLEIYMDDQ------ELCIKDTG 254 Query: 263 IGISSEDLKRVFNPYFTGERGRQYHESTGMGLYLVREICSRLGHKVGLESKPGEGTTVRI 322 IGI + D+ RVF F+G GR +S+G+GLYL ++I LGH++ +ES+ G+GTTVRI Sbjct: 255 IGIKNSDVLRVFERGFSGYNGRLTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRI 314 Query: 323 IFNGTGL 329 F L Sbjct: 315 QFAQVNL 321 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,471,487 Number of extensions: 926592 Number of successful extensions: 4045 Number of sequences better than 1.0e-05: 177 Number of HSP's gapped: 3908 Number of HSP's successfully gapped: 177 Length of query: 330 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 223 Effective length of database: 26,037,693 Effective search space: 5806405539 Effective search space used: 5806405539 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)