BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0022.p (361 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc3896 [E] COG3842 ABC-type spermidine/putrescine transport sys... 291 2e-78 ECs0413 [E] COG3842 ABC-type spermidine/putrescine transport sys... 287 1e-77 ZafuC [E] COG3842 ABC-type spermidine/putrescine transport syste... 286 5e-77 afuC [E] COG3842 ABC-type spermidine/putrescine transport system... 283 2e-76 ZafuC [E] COG3842 ABC-type spermidine/putrescine transport syste... 283 2e-76 >AGc3896 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 182 Score = 291 bits (744), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 0/328 (0%), Positives = 0/328 (0%), Gaps = 21/328 (6%) Query: 1 MSQLIIENLVKNYGQKPVVNNLNITVESGEMLSLLGPSGCGKTTTLRMIAGMHDPTSGSI 60 M + L + + L + + Sbjct: 1 MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPG 60 Query: 61 RLDGRELTTIAPHKRNIGLVFQNYALFPHLSVFENVAFGLKRHGVQKSEIRGRVEAALHS 120 + N V + + + + Sbjct: 61 VWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPV 120 Query: 121 VRLNDFAERFPAQMSGGQQQRVALARTLVLKPPLVLFDEPLSNLDAKLRQALGIEIRVLQ 180 + Q LA L P + A R+A Q Sbjct: 121 FAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVMQATNREARKRLSAFTQ 180 Query: 181 REYGFTGIFVTHDQEEAMVISDRIAVMNGGNIVQTGTPQQIYMHPADPFVADFVGESNLF 240 Sbjct: 181 240 Query: 241 PITNIMESEGMWRF---ELTKGNPISAEKTGNGSAPTYVMVRPEAVSVRRKSLEPHPVSG 297 Sbjct: 241 ------------ 288 Query: 298 HGLNTLAGEVAFINYSGSSYLIGVVIKS 325 Sbjct: 289 ------ 310 >ECs0413 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 352 Score = 287 bits (735), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 147/347 (42%), Positives = 223/347 (64%), Gaps = 20/347 (5%) Query: 6 IENLVKNYGQKPVVNNLNITVESGEMLSLLGPSGCGKTTTLRMIAGMHDPTSGSIRLDGR 65 + N+ K +G V++N+N+T+ G+M++LLGPSGCGKTT LR++AG+ P+ G I +DG Sbjct: 13 LRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDGE 72 Query: 66 ELTTIAPHKRNIGLVFQNYALFPHLSVFENVAFGLKRHGVQKSEIRGRVEAALHSVRLND 125 ++T + +R+I +VFQ+YALFPH+S+ ENV +GLK GV +SE++ RV+ AL V L Sbjct: 73 DVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVSRSEVKQRVKEALAMVDLEG 132 Query: 126 FAERFPAQMSGGQQQRVALARTLVLKPPLVLFDEPLSNLDAKLRQALGIEIRVLQREYGF 185 F +R+ Q+SGGQQQRVALAR L+LKP ++LFDEPLSNLDA LR+++ +IR LQ+++ Sbjct: 133 FEDRYVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIRELQKQFNI 192 Query: 186 TGIFVTHDQEEAMVISDRIAVMNGGNIVQTGTPQQIYMHPADPFVADFVGESNLFPITNI 245 T ++VTHDQ EA +SD + VMN G+I+Q G+PQ +Y PA F+A F+G++NLFP Sbjct: 193 TSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDANLFPANFS 252 Query: 246 MESEGMWRFELTKGNPISAEKTGNGSAPTYVMVRPEAVSVRRKSLEPHPVSGHGLNTLAG 305 E ++ + L + + +G V VRPEA++ +S HG + Sbjct: 253 EEYVDIYGYRLPRAAHFPVQGSGT------VGVRPEAIT----------LSNHGEESQRC 296 Query: 306 EVAFINYSGSSYLIGVVIKSLDQQFIIRLQNTRRELNLRVGEEAVVE 352 + + Y G Y V ++ Q+ ++++ TR L +GE +E Sbjct: 297 VIRHVAYMGPQY--EVTVEWHGQEILLQVNATR--LQPDIGEHYYLE 339 >ZafuC [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 352 Score = 286 bits (731), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 146/347 (42%), Positives = 222/347 (63%), Gaps = 20/347 (5%) Query: 6 IENLVKNYGQKPVVNNLNITVESGEMLSLLGPSGCGKTTTLRMIAGMHDPTSGSIRLDGR 65 + N+ K +G V++N+N+T+ G+M++LLGPSGCGKTT LR++AG+ P+ G I +DG Sbjct: 13 LRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDGE 72 Query: 66 ELTTIAPHKRNIGLVFQNYALFPHLSVFENVAFGLKRHGVQKSEIRGRVEAALHSVRLND 125 ++T + +R+I +VFQ+YALFPH+S+ ENV +GLK GV +SE++ RV+ AL V Sbjct: 73 DVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVSRSEVKQRVKEALAXVDXEG 132 Query: 126 FAERFPAQMSGGQQQRVALARTLVLKPPLVLFDEPLSNLDAKLRQALGIEIRVLQREYGF 185 F +R+ Q+SGGQQQRVALAR L+LKP ++LFDEPLSNLDA LR+++ +IR LQ+++ Sbjct: 133 FEDRYVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIRELQKQFNI 192 Query: 186 TGIFVTHDQEEAMVISDRIAVMNGGNIVQTGTPQQIYMHPADPFVADFVGESNLFPITNI 245 T ++VTHDQ EA +SD + VMN G+I+Q G+PQ +Y PA F+A F+G++NLFP Sbjct: 193 TSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDANLFPANFS 252 Query: 246 MESEGMWRFELTKGNPISAEKTGNGSAPTYVMVRPEAVSVRRKSLEPHPVSGHGLNTLAG 305 E ++ + L + + +G V VRPEA++ +S HG + Sbjct: 253 EEYVDIYGYRLPRAAHFPVQGSGT------VGVRPEAIT----------LSNHGEESQRC 296 Query: 306 EVAFINYSGSSYLIGVVIKSLDQQFIIRLQNTRRELNLRVGEEAVVE 352 + + Y G Y V ++ Q+ ++++ TR L +GE +E Sbjct: 297 VIRHVAYMGPQY--EVTVEWHGQEILLQVNATR--LQPDIGEHYYLE 339 >afuC [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 352 Score = 283 bits (725), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 149/347 (42%), Positives = 224/347 (64%), Gaps = 20/347 (5%) Query: 6 IENLVKNYGQKPVVNNLNITVESGEMLSLLGPSGCGKTTTLRMIAGMHDPTSGSIRLDGR 65 + N+ K +G V++N+N+T+ G+M++LLGPSGCGKTT LR++AG+ P+ G I +DG Sbjct: 13 LRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDGE 72 Query: 66 ELTTIAPHKRNIGLVFQNYALFPHLSVFENVAFGLKRHGVQKSEIRGRVEAALHSVRLND 125 ++T + +R+I +VFQ+YALFPH+S+ ENV +GLK GV ++E++ RV+ AL V L Sbjct: 73 DVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVPRAELKARVKEALAMVDLEG 132 Query: 126 FAERFPAQMSGGQQQRVALARTLVLKPPLVLFDEPLSNLDAKLRQALGIEIRVLQREYGF 185 F +RF Q+SGGQQQRVALAR L+LKP ++LFDEPLSNLDA LR+++ +IR LQ+++ Sbjct: 133 FEDRFVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIRELQKQFDI 192 Query: 186 TGIFVTHDQEEAMVISDRIAVMNGGNIVQTGTPQQIYMHPADPFVADFVGESNLFPITNI 245 T ++VTHDQ EA +SD + VMN G+I+Q G+PQ +Y PA F+A F+G++NLFP T Sbjct: 193 TSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDANLFPATFS 252 Query: 246 MESEGMWRFELTKGNPISAEKTGNGSAPTYVMVRPEAVSVRRKSLEPHPVSGHGLNTLAG 305 ++ + L + P+ G G V VRPEA++ +S G + Sbjct: 253 DGYVDIYGYHLPR--PLHFGTQGEG----MVGVRPEAIT----------LSDRGEESQRC 296 Query: 306 EVAFINYSGSSYLIGVVIKSLDQQFIIRLQNTRRELNLRVGEEAVVE 352 + + Y G Y V ++ Q+ ++++ TR L VGE+ +E Sbjct: 297 VIRHVAYMGPQY--EVTVEWHGQEILLQVNATR--LQPDVGEQYYLE 339 Score = 283 bits (725), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 147/347 (42%), Positives = 222/347 (63%), Gaps = 20/347 (5%) Query: 6 IENLVKNYGQKPVVNNLNITVESGEMLSLLGPSGCGKTTTLRMIAGMHDPTSGSIRLDGR 65 + N+ K +G V++N+N+T+ G+M++LLGPSGCGKTT LR++AG+ P+ G I +DG Sbjct: 13 LRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDGE 72 Query: 66 ELTTIAPHKRNIGLVFQNYALFPHLSVFENVAFGLKRHGVQKSEIRGRVEAALHSVRLND 125 ++T + +R+I +VFQ+YALFPH+S+ ENV +GLK GV +SE++ RV+ AL V Sbjct: 73 DVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVSRSEVKQRVKEALAXVDXEG 132 Query: 126 FAERFPAQMSGGQQQRVALARTLVLKPPLVLFDEPLSNLDAKLRQALGIEIRVLQREYGF 185 F +R+ Q+SGGQQQRVALAR L+LKP ++LFDEPLSNLDA LR+++ +IR LQ+++ Sbjct: 133 FEDRYVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIRELQKQFNI 192 Query: 186 TGIFVTHDQEEAMVISDRIAVMNGGNIVQTGTPQQIYMHPADPFVADFVGESNLFPITNI 245 T ++VTHDQ EA +SD + VMN G+I+Q G+PQ +Y PA F+A F+G++NLFP Sbjct: 193 TSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDANLFPANFS 252 Query: 246 MESEGMWRFELTKGNPISAEKTGNGSAPTYVMVRPEAVSVRRKSLEPHPVSGHGLNTLAG 305 E ++ + L + G+G V VRPEA++ +S HG + Sbjct: 253 EEYVDIYGYRLPR--AAHFPVQGSG----TVGVRPEAIT----------LSNHGEESQRC 296 Query: 306 EVAFINYSGSSYLIGVVIKSLDQQFIIRLQNTRRELNLRVGEEAVVE 352 + + Y G Y V ++ Q+ ++++ TR L +GE +E Sbjct: 297 VIRHVAYMGPQY--EVTVEWHGQEILLQVNATR--LQPDIGEHYYLE 339 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,102,817 Number of extensions: 1121909 Number of successful extensions: 9274 Number of sequences better than 1.0e-05: 2209 Number of HSP's gapped: 6111 Number of HSP's successfully gapped: 2458 Length of query: 361 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 253 Effective length of database: 25,922,359 Effective search space: 6558356827 Effective search space used: 6558356827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)