BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0021.p (241 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport sys... 115 5e-26 PA0606 [E] COG1177 ABC-type spermidine/putrescine transport syst... 114 1e-25 YPO2841 [E] COG1177 ABC-type spermidine/putrescine transport sys... 107 1e-23 AGl888 [E] COG1177 ABC-type spermidine/putrescine transport syst... 102 4e-22 AGl1232 [E] COG1177 ABC-type spermidine/putrescine transport sys... 99 7e-21 >SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 266 Score = 115 bits (288), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 59/216 (27%), Positives = 112/216 (51%) Query: 3 PLLVILISSFNSGEFLVFPPKGFSLRWYEEVLTGGRYTGPFLTSIKLAVLTTLFALPFGT 62 P+L I + SF S ++L+FPP G++LRWY+E+L R+ TS ++A++ T+ ++ G Sbjct: 29 PILFIAVLSFGSSQWLIFPPPGWTLRWYQELLEDPRWLDSAWTSFRIAIIVTVLSVLLGL 88 Query: 63 MIAYAIQRFEFRLKNALQGLFLSPLVIPTXXXXXXXXXXXXXXXXRMFFXXXXXXXXXXX 122 + ++ + R F + AL+ LFL+P+++P Sbjct: 89 VTSFGLNRGRFLFREALKALFLTPMILPVVVLAVALYAFFLQIGLNGTLTGFVISHLVLA 148 Query: 123 XPYVVRTMLAGFSRMERSVEEASIILGASPVKTFFLVTLPFARPXXXXXXXXXXXXXXDE 182 P+ + ++ ++S+E+A+++ GASP + VTLP DE Sbjct: 149 LPFSILSITNALEGFDKSIEDAAVLCGASPFEAKIRVTLPAISHGVFSAAIFSFLTSWDE 208 Query: 183 LVIALFLTGPGINTLPMTIYSDIQFNLSPSLAAVSS 218 +V+A+F+ P + TLP+ ++S ++ +L+P +AA S+ Sbjct: 209 VVVAIFMASPTLQTLPVKVWSTLRQDLTPVVAAAST 244 >PA0606 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 276 Score = 114 bits (285), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 71/208 (34%), Positives = 101/208 (48%) Query: 11 SFNSGEFLVFPPKGFSLRWYEEVLTGGRYTGPFLTSIKLAVLTTLFALPFGTMIAYAIQR 70 SFNSG FLV+P +GFSLRWY + + S+ +A TL A+ FGT+ A + R Sbjct: 39 SFNSGTFLVYPLQGFSLRWYADFFQSAEWMRSLTNSMIVAPAATLLAMVFGTLAAIGLTR 98 Query: 71 FEFRLKNALQGLFLSPLVIPTXXXXXXXXXXXXXXXXRMFFXXXXXXXXXXXXPYVVRTM 130 EFR K + L +SP+V+P + P+V+ T+ Sbjct: 99 GEFRGKALVMSLMISPMVVPVVIIGVASYLFFAPLGLGNSYLSLIVVHAVLGVPFVIITV 158 Query: 131 LAGFSRMERSVEEASIILGASPVKTFFLVTLPFARPXXXXXXXXXXXXXXDELVIALFLT 190 A ++ A+ LGA P+ TFF VTLP P DE+V+ LFL Sbjct: 159 SATLQGFNYNLVRAAASLGAPPLTTFFRVTLPLIAPGVISGALFAFATSFDEVVVTLFLA 218 Query: 191 GPGINTLPMTIYSDIQFNLSPSLAAVSS 218 GP TLP ++S I+ NLSP++AA ++ Sbjct: 219 GPEQATLPRQMFSGIRENLSPTIAAAAT 246 >YPO2841 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 282 Score = 107 bits (268), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 69/218 (31%), Positives = 103/218 (47%) Query: 1 MAPLLVILISSFNSGEFLVFPPKGFSLRWYEEVLTGGRYTGPFLTSIKLAVLTTLFALPF 60 + P+LVI+ SFN+G FL +P GFSLRWY+ + G S+ +A L TL A Sbjct: 35 IVPVLVIVPLSFNAGSFLSYPMAGFSLRWYQTFFNSDEWLGALGNSLLIAPLATLLATGL 94 Query: 61 GTMIAYAIQRFEFRLKNALQGLFLSPLVIPTXXXXXXXXXXXXXXXXRMFFXXXXXXXXX 120 G + A + R EFR K + + +SP+V P + Sbjct: 95 GVLAAIGLVRGEFRGKALVMAMLISPMVAPVVIIAVGMFFFFARLSLLNSYLGLVLAHAL 154 Query: 121 XXXPYVVRTMLAGFSRMERSVEEASIILGASPVKTFFLVTLPFARPXXXXXXXXXXXXXX 180 P+VV T+ A ++++ A+ LGAS + F VTLP P Sbjct: 155 LGVPFVVITVTAVLKSYDQNLSRAAASLGASQWRVFRKVTLPLIAPGVFSGALFAFAVSF 214 Query: 181 DELVIALFLTGPGINTLPMTIYSDIQFNLSPSLAAVSS 218 DE+V+ LFL P TLP+ +++ I+ NL P++AA +S Sbjct: 215 DEVVVTLFLASPRQRTLPIKMFAGIRENLDPTIAAAAS 252 >AGl888 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 832 Score = 102 bits (255), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 10/218 (4%), Positives = 27/218 (12%) Query: 1 MAPLLVILISSFNSGEFLVFPPKGFSLRWYEEVLTGGRYTGPFLTSIKLAVLTTLFALPF 60 LL+ + F + L+ Sbjct: 19 DCQLLLGCPPPAANITRQQFNVSIVVRHKPVLKPVLEPFCLCRLSGRNGGQFIGNILRRA 78 Query: 61 GTMIAYAIQRFEFRLKNALQGLFLSPLVIPTXXXXXXXXXXXXXXXXRMFFXXXXXXXXX 120 + ++ + Sbjct: 79 EGGQLSLGWKEQWDNLDFSHITVGVLSQAYEYYLRKHAPKQQQQEGGFYTPHPIANLMVS 138 Query: 121 XXXPYVVRTMLAGFSRMERSVEEASIILGASPVKTFFLVTLPFARPXXXXXXXXXXXXXX 180 + ++ +R+ A + L A+ + Sbjct: 139 AAFRGLNAQGISAAARILDPAAGAGVFLLAAFRELVAARWRSEGHRPDTAALREILYKQV 198 Query: 181 DELVIALFLTGPGINTLPMTIYSDIQFNLSPSLAAVSS 218 I L + Sbjct: 199 RGFDINEAALRFCALGLYLLSIELDPNPKPVDKLHFDD 236 >AGl1232 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 134 Score = 99.0 bits (245), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 0/216 (0%), Positives = 0/216 (0%) Query: 3 PLLVILISSFNSGEFLVFPPKGFSLRWYEEVLTGGRYTGPFLTSIKLAVLTTLFALPFGT 62 + ++ + + K ++ + L+ + Sbjct: 29 SGVPMVTAVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSG 88 Query: 63 MIAYAIQRFEFRLKNALQGLFLSPLVIPTXXXXXXXXXXXXXXXXRMFFXXXXXXXXXXX 122 Sbjct: 89 148 Query: 123 XPYVVRTMLAGFSRMERSVEEASIILGASPVKTFFLVTLPFARPXXXXXXXXXXXXXXDE 182 Sbjct: 149 208 Query: 183 LVIALFLTGPGINTLPMTIYSDIQFNLSPSLAAVSS 218 Sbjct: 209 244 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,974,358 Number of extensions: 371827 Number of successful extensions: 1592 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 1561 Number of HSP's successfully gapped: 55 Length of query: 241 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 138 Effective length of database: 26,499,029 Effective search space: 3656866002 Effective search space used: 3656866002 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 114 (48.5 bits)