BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0020.p (285 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport sys... 171 1e-42 STM1225 [E] COG1176 ABC-type spermidine/putrescine transport sys... 155 1e-37 potB [E] COG1176 ABC-type spermidine/putrescine transport system... 154 1e-37 ZpotB [E] COG1176 ABC-type spermidine/putrescine transport syste... 154 1e-37 >SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 296 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 88/231 (38%), Positives = 140/231 (60%) Query: 50 WQQYGRFFTDPFYLQMLWRSVYIGVAVSFICLILGYCLAYGVSRAQPKHHFXXXXXXXXX 109 + Y R FT+P +L++L+ ++ + V+ L+LG+ +AY ++R Sbjct: 60 FSHYRRIFTEPLHLEVLFSTIEVAFVVTVAGLLLGFPVAYLMARLSRGLAMAVAACVFVP 119 Query: 110 XXTSAVIRNFGWIIILGRKGILNQFLLGIGLIQEPLELMYTPAGVIIALVHVMLPYMVLV 169 TS +IR++ W+++L R GI+N+ L G+ + PL+L+YT VI+A+ HV++P+M+L Sbjct: 120 LWTSVLIRSYAWVVLLQRNGIVNKLLADTGVTEGPLKLIYTQGAVILAMTHVLMPFMILP 179 Query: 170 LYSVLEGMDRNLESAAANLGASKTKVFWFVTLPLSKQGIIAGTLLVFSITLSFFVTPSLI 229 +YS L + + AA NLGA + F VTLPLS GI AG+++ F + L F++TP+L+ Sbjct: 180 IYSALRALPPDYVRAARNLGAGPIRAFVTVTLPLSLPGIFAGSVMCFVLALGFYITPALV 239 Query: 230 GGAKVKLMATEIYNQTINLLNWPYASAMGVILLLTMLGVTSLYRRMLSGRK 280 GG LMAT I QT LL+WP+A+A+ +LL L ++RR LS K Sbjct: 240 GGPSSMLMATLIGQQTTVLLDWPFAAALSTVLLAVTLLFVLMFRRTLSLSK 290 >STM1225 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 287 Score = 155 bits (391), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 84/232 (36%), Positives = 133/232 (57%), Gaps = 1/232 (0%) Query: 34 SFYQTDSSGGMEAAFVWQQYGRFFTDPFYLQMLWRSVYIGVAVSFICLILGYCLAYGVSR 93 SF D + ++ F Y R DP Y ++L S+ + + + CL+LGY A+ +++ Sbjct: 34 SFLTRDDASFVKMVFTLDNYARLL-DPLYFEVLLHSLNMALIATLSCLVLGYPFAWFLAK 92 Query: 94 AQPKHHFXXXXXXXXXXXTSAVIRNFGWIIILGRKGILNQFLLGIGLIQEPLELMYTPAG 153 K T+++IR +G I L KG LN+FLL +G+I P+ +M+TP+ Sbjct: 93 LPEKIRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSA 152 Query: 154 VIIALVHVMLPYMVLVLYSVLEGMDRNLESAAANLGASKTKVFWFVTLPLSKQGIIAGTL 213 VII LV+++LP+MV+ LYS +E +D+ L AA +LGASK + F + +PL+ GI+AG L Sbjct: 153 VIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGASKMQTFIRIIIPLTMPGIVAGCL 212 Query: 214 LVFSITLSFFVTPSLIGGAKVKLMATEIYNQTINLLNWPYASAMGVILLLTM 265 LV + F L+GGAK L+ I Q +N+ +WP+ +A + L + M Sbjct: 213 LVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVM 264 >potB [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 275 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 85/232 (36%), Positives = 133/232 (57%), Gaps = 1/232 (0%) Query: 34 SFYQTDSSGGMEAAFVWQQYGRFFTDPFYLQMLWRSVYIGVAVSFICLILGYCLAYGVSR 93 SF D + ++ F Y R DP Y ++L S+ + + + CL+LGY A+ +++ Sbjct: 24 SFLTRDDASFVKMVFTLDNYTRLL-DPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAK 82 Query: 94 AQPKHHFXXXXXXXXXXXTSAVIRNFGWIIILGRKGILNQFLLGIGLIQEPLELMYTPAG 153 K T+++IR +G I L KG LN+FLL +G+I P+ +M+TP+ Sbjct: 83 LPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSA 142 Query: 154 VIIALVHVMLPYMVLVLYSVLEGMDRNLESAAANLGASKTKVFWFVTLPLSKQGIIAGTL 213 VII LV+++LP+MV+ LYS +E +D+ L AA +LGASK + F + +PL+ GIIAG L Sbjct: 143 VIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCL 202 Query: 214 LVFSITLSFFVTPSLIGGAKVKLMATEIYNQTINLLNWPYASAMGVILLLTM 265 LV + F L+GGAK L+ I Q +N+ +WP+ +A + L + M Sbjct: 203 LVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVM 254 >ZpotB [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 275 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 85/232 (36%), Positives = 133/232 (57%), Gaps = 1/232 (0%) Query: 34 SFYQTDSSGGMEAAFVWQQYGRFFTDPFYLQMLWRSVYIGVAVSFICLILGYCLAYGVSR 93 SF D + ++ F Y R DP Y ++L S+ + + + CL+LGY A+ +++ Sbjct: 24 SFLTRDDASFVKMVFTLDNYTRLL-DPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAK 82 Query: 94 AQPKHHFXXXXXXXXXXXTSAVIRNFGWIIILGRKGILNQFLLGIGLIQEPLELMYTPAG 153 K T+++IR +G I L KG LN+FLL +G+I P+ +M+TP+ Sbjct: 83 LPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSA 142 Query: 154 VIIALVHVMLPYMVLVLYSVLEGMDRNLESAAANLGASKTKVFWFVTLPLSKQGIIAGTL 213 VII LV+++LP+MV+ LYS +E +D+ L AA +LGASK + F + +PL+ GIIAG L Sbjct: 143 VIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCL 202 Query: 214 LVFSITLSFFVTPSLIGGAKVKLMATEIYNQTINLLNWPYASAMGVILLLTM 265 LV + F L+GGAK L+ I Q +N+ +WP+ +A + L + M Sbjct: 203 LVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVM 254 Score = 154 bits (389), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 8/232 (3%), Positives = 33/232 (14%), Gaps = 1/232 (0%) Query: 34 SFYQTDSSGGMEAAFVWQQYGRFFTDPFYLQMLWRSVYIGVAVSFICLILGYCLAYGVSR 93 + + + L + Sbjct: 34 VKMVFTLDNYTRLLDPLYFEVLLH-SLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL 92 Query: 94 AQPKHHFXXXXXXXXXXXTSAVIRNFGWIIILGRKGILNQFLLGIGLIQEPLELMYTPAG 153 F + L G+++ + + + + Sbjct: 93 FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYILL 152 Query: 154 VIIALVHVMLPYMVLVLYSVLEGMDRNLESAAANLGASKTKVFWFVTLPLSKQGIIAGTL 213 + + + + + + L G Sbjct: 153 PFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLF 212 Query: 214 LVFSITLSFFVTPSLIGGAKVKLMATEIYNQTINLLNWPYASAMGVILLLTM 265 V + L + + + +++ Sbjct: 213 YVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRA 264 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,501,766 Number of extensions: 709657 Number of successful extensions: 8480 Number of sequences better than 1.0e-05: 172 Number of HSP's gapped: 8463 Number of HSP's successfully gapped: 175 Length of query: 285 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 180 Effective length of database: 26,268,361 Effective search space: 4728304980 Effective search space used: 4728304980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)