BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0017.p (389 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO2553 [R] COG2159 Predicted metal-dependent hydrolase of the T... 160 3e-39 Rv1879 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 86 9e-17 MT1928 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 86 9e-17 >SSO2553 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 339 Score = 160 bits (405), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 105/342 (30%), Positives = 181/342 (52%), Gaps = 35/342 (10%) Query: 43 TRNTL--LFKIVRKEMIQLLGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLL 100 T N+L + +R E+ LLG++ I +RN+ + +P Y+ +LF D G++ + Sbjct: 25 TMNSLRPFYLYLRNELKLLLGVN-------FIEERNKLIKNDPVNYMRLLFDDAGIDGFV 77 Query: 101 VDYGFPVMGSRLAQDELDWFHQTSSSIPTRR--IHRVEATYHR-LFEEKVPFGELEETYI 157 +D GF + E++ IP + + R+E+ + +F+ + F + E + Sbjct: 78 IDTGF-------GKKEME--------IPAKLKLLFRIESIINDDIFQ--MSFDKALEYFE 120 Query: 158 RELRDEVENQGTIAFKTVIAYHTGLKINKISKSQAAASYSRFLESPDNTEAEKDVRDYLV 217 LR ++ +G FK++IAY TGLK+N + +S E + K RDYL+ Sbjct: 121 ETLRRKITKEGYAGFKSIIAYRTGLKVNCNIEEARRDFHSN--EKDWFGKVAKGFRDYLL 178 Query: 218 LLAFELCMEYDIPIQMHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFV 277 + E +P+Q+H+GAGD + ++L+ P + +IV Y+ K+IF+H YP+ Sbjct: 179 CETLRIAKELRVPVQIHTGAGDRDI-KLELSRPSYLTNIVR--KYEG-KVIFVHAGYPYH 234 Query: 278 EEAGYLVNQYHNVYLDLSSMIPFSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLG 337 E ++ + +VYLD S +IPF+ + L +IFE+AP KVM+GSD F IPE+SWL Sbjct: 235 RETAWMSYLFPSVYLDTSQVIPFAPLAAYNILNEIFEVAPLNKVMHGSDAFHIPEISWLA 294 Query: 338 GRMIKKELGKMLTDLVTDGIIDEEYAYTAAGLVLSENARNLY 379 ++ KK + K ++ I+DE++A L N++ +Y Sbjct: 295 AKLAKKAIQKTTELMIEKNILDEKHAKEMVERFLYYNSKEMY 336 >Rv1879 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 378 Score = 85.9 bits (211), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 88/364 (24%), Positives = 151/364 (41%), Gaps = 36/364 (9%) Query: 1 MTIDISRLPIIDDHCHP-FMPTREKKSFDNCFTLSLIPPKSR--DTRNTLLFKIVRKEMI 57 +T + +P+ID H H ++ ++ F+N + P + ++ L VR Sbjct: 9 LTRHTAEVPLIDQHVHGCWLTEGNRRRFENALNEANTEPLADFDSGFDSQLGFAVRNHCA 68 Query: 58 QLLGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPV----MGSRLA 113 +LGL PR D + R R Q + E + G+ LV+ G M S Sbjct: 69 PILGL-PRHVDPQTYWDR--RSQFSEAELARRFLQAAGVTDWLVETGIGYDVSGMASVAG 125 Query: 114 QDELDWFHQTSSSIPTRRIHRVEATYHRLFEEKVPFGELEETYIRELRDEVENQGTIAFK 173 EL H + R+E + + G+ + LR +A K Sbjct: 126 LGELSGSH-------AHEVVRLEQVAEQAVQAS---GDYASAFNEILRRRAAT--AVATK 173 Query: 174 TVIAYHTGLK--INKISKSQAAASYSRFLESPDNTEAEKDVRDYLVLLAFEL--CMEYDI 229 +++AY G + + +Q A + R+ + ++ VLL F L + Sbjct: 174 SILAYRGGFDGDLTEPPAAQVAEAAKRWRDRGGVRLQDR------VLLRFGLHQALRLGK 227 Query: 230 PIQMHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLVNQYHN 289 P+Q H G GD D+ A+PL + + I+ +H YP+ EAGYL ++N Sbjct: 228 PLQFHVGFGDRDA-DLHKANPLYLLDFLRQS--GNTPIVLLHC-YPYEREAGYLAQAFNN 283 Query: 290 VYLDLSSMIPFSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKELGKML 349 VYLD + + + ++ E+APF K++Y SDGF E+ +LG + + + ++L Sbjct: 284 VYLDGGLSVHYLGARSPAFIGRLLELAPFRKIVYSSDGFGPAELHFLGATLWRSGIQRVL 343 Query: 350 TDLV 353 V Sbjct: 344 RGFV 347 >MT1928 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 378 Score = 85.9 bits (211), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 88/364 (24%), Positives = 151/364 (41%), Gaps = 36/364 (9%) Query: 1 MTIDISRLPIIDDHCHP-FMPTREKKSFDNCFTLSLIPPKSR--DTRNTLLFKIVRKEMI 57 +T + +P+ID H H ++ ++ F+N + P + ++ L VR Sbjct: 9 LTRHTAEVPLIDQHVHGCWLTEGNRRRFENALNEANTEPLADFDSGFDSQLGFAVRNHCA 68 Query: 58 QLLGLDPRATDDELILQRNQRYQANPKEYVDMLFKDVGLESLLVDYGFPV----MGSRLA 113 +LGL PR D + R R Q + E + G+ LV+ G M S Sbjct: 69 PILGL-PRHVDPQTYWDR--RSQFSEAELARRFLQAAGVTDWLVETGIGYDVSGMASVAG 125 Query: 114 QDELDWFHQTSSSIPTRRIHRVEATYHRLFEEKVPFGELEETYIRELRDEVENQGTIAFK 173 EL H + R+E + + G+ + LR +A K Sbjct: 126 LGELSGSH-------AHEVVRLEQVAEQAVQAS---GDYASAFNEILRRRAAT--AVATK 173 Query: 174 TVIAYHTGLK--INKISKSQAAASYSRFLESPDNTEAEKDVRDYLVLLAFEL--CMEYDI 229 +++AY G + + +Q A + R+ + ++ VLL F L + Sbjct: 174 SILAYRGGFDGDLTEPPAAQVAEAAKRWRDRGGVRLQDR------VLLRFGLHQALRLGK 227 Query: 230 PIQMHSGAGDAPLLDVKLASPLLMHSIVSDPHYQAVKIIFIHTAYPFVEEAGYLVNQYHN 289 P+Q H G GD D+ A+PL + + I+ +H YP+ EAGYL ++N Sbjct: 228 PLQFHVGFGDRDA-DLHKANPLYLLDFLRQS--GNTPIVLLHC-YPYEREAGYLAQAFNN 283 Query: 290 VYLDLSSMIPFSSIGVQPKLRQIFEMAPFTKVMYGSDGFTIPEVSWLGGRMIKKELGKML 349 VYLD + + + ++ E+APF K++Y SDGF E+ +LG + + + ++L Sbjct: 284 VYLDGGLSVHYLGARSPAFIGRLLELAPFRKIVYSSDGFGPAELHFLGATLWRSGIQRVL 343 Query: 350 TDLV 353 V Sbjct: 344 RGFV 347 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,216,359 Number of extensions: 1206414 Number of successful extensions: 1817 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 1816 Number of HSP's successfully gapped: 3 Length of query: 389 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 281 Effective length of database: 25,922,359 Effective search space: 7284182879 Effective search space used: 7284182879 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)