BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0012.p (328 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yulF [R] COG0673 Predicted dehydrogenases and related proteins 407 e-113 CAC1231 [R] COG0673 Predicted dehydrogenases and related proteins 366 e-101 ZygjR [R] COG0673 Predicted dehydrogenases and related proteins 302 6e-82 ECs3969 [R] COG0673 Predicted dehydrogenases and related proteins 302 6e-82 ZygjR [R] COG0673 Predicted dehydrogenases and related proteins 300 2e-81 >BS_yulF [R] COG0673 Predicted dehydrogenases and related proteins Length = 328 Score = 407 bits (1046), Expect = e-113, Method: Compositional matrix adjust. Identities = 184/325 (56%), Positives = 249/325 (76%) Query: 3 IRFGVIGTNWITDRFIQAGLENEEFILTAVYSRTEEKGRDFAAKYAGASIYTNLEKMVAS 62 IRF +IGTNWITDRF+++ + E+F LTAVYSR+ E+ +FAAK+ A +++L++M AS Sbjct: 2 IRFAIIGTNWITDRFLESAADIEDFQLTAVYSRSAERAGEFAAKHNAAHAFSDLQEMAAS 61 Query: 63 EEVDAVYIASPNSMHAEHALVCMNHGKHVICEKPAASNSAELRAMIEAARDNDVLFMEAM 122 + DAVYIASPN++H E A++ MNHGKHV+CEKP ASN+ E MI AA+ N V+ MEAM Sbjct: 62 DCFDAVYIASPNALHKEQAVLFMNHGKHVLCEKPFASNTKETEEMISAAKANGVVLMEAM 121 Query: 123 KSTFMPNFGVIQDNLYKLGQVRRYFAGYCQYSSRYDAYRQGTVLNAFNPAFSNGSLMDLG 182 K+TF+PNF ++ +L+K+G VRR+ A YCQYSSRYDA+R GTVLNAF P SNGSLMD+G Sbjct: 122 KTTFLPNFKELKKHLHKIGTVRRFTASYCQYSSRYDAFRSGTVLNAFQPELSNGSLMDIG 181 Query: 183 IYCLYPMVALFGKPESVRAVGLMLASGVDGEGSIVMRYPDMDAVVMHSKIADSYLPAEIQ 242 +YC+YP V LFG P+ V+A G L+SGVDGEG++++ Y +AV+MHSKI+ SY PAEIQ Sbjct: 182 VYCIYPAVVLFGAPKDVKANGYALSSGVDGEGTVILSYDGFEAVLMHSKISTSYAPAEIQ 241 Query: 243 GENGTMVIDKINQPYQVKIHYRDGTVEELTLPQVFEPMYYEVEEFIRLIRSGQRESSVNS 302 GE+GT+VID I++P +V+I YRDG +E + +P M+YE EEF+ LI+ + ES N+ Sbjct: 242 GEDGTIVIDTIHRPERVEIRYRDGRLENIAIPDPKPAMFYEAEEFVTLIKENKLESEENT 301 Query: 303 HANSLAVAEVMEEARAQIGLRYASD 327 SL A++MEEAR Q+G+ Y +D Sbjct: 302 FERSLTTAKIMEEARKQMGIVYPAD 326 >CAC1231 [R] COG0673 Predicted dehydrogenases and related proteins Length = 331 Score = 366 bits (940), Expect = e-101, Method: Compositional matrix adjust. Identities = 167/326 (51%), Positives = 240/326 (73%) Query: 2 TIRFGVIGTNWITDRFIQAGLENEEFILTAVYSRTEEKGRDFAAKYAGASIYTNLEKMVA 61 TIRFG++GT+ IT++F+ ++F LTAVYSR E K RDFA KY +I+T+LEKM Sbjct: 4 TIRFGIVGTSSITEKFLDGSNHVKDFSLTAVYSREESKARDFAKKYGAENIFTDLEKMAK 63 Query: 62 SEEVDAVYIASPNSMHAEHALVCMNHGKHVICEKPAASNSAELRAMIEAARDNDVLFMEA 121 S+ +DAVYIASPN+MHA+ A V + + KHV+CEK ASN E+ MI +++N ++ MEA Sbjct: 64 SDLIDAVYIASPNAMHAKQAEVFLKNKKHVLCEKAFASNVEEVNKMISVSKENKMVLMEA 123 Query: 122 MKSTFMPNFGVIQDNLYKLGQVRRYFAGYCQYSSRYDAYRQGTVLNAFNPAFSNGSLMDL 181 MK+ ++P F +++NL+++G+VR+YF YCQYSSRYD ++QG VLNAF P SNGS+MD+ Sbjct: 124 MKTEYLPGFFAVKENLHRIGKVRKYFGNYCQYSSRYDKFKQGIVLNAFKPELSNGSIMDI 183 Query: 182 GIYCLYPMVALFGKPESVRAVGLMLASGVDGEGSIVMRYPDMDAVVMHSKIADSYLPAEI 241 G+YC++PMV LFG+P ++A ++L SGVDGEGSI+ Y +M+AVVM+SKI +S + +EI Sbjct: 184 GVYCIHPMVYLFGEPNKLKANAVLLESGVDGEGSILFNYDNMEAVVMYSKITNSRVSSEI 243 Query: 242 QGENGTMVIDKINQPYQVKIHYRDGTVEELTLPQVFEPMYYEVEEFIRLIRSGQRESSVN 301 QGE G +VID IN +VKI +R+G E+L+ + + M YE+EEFIRL++ + ++ Sbjct: 244 QGEEGNIVIDNINTFEKVKIVFRNGEEEDLSRVHIEDNMCYELEEFIRLVKEQDTKKALQ 303 Query: 302 SHANSLAVAEVMEEARAQIGLRYASD 327 S +V E+M+EAR QIGL + +D Sbjct: 304 RLKVSKSVMEIMDEARKQIGLVFPAD 329 >ZygjR [R] COG0673 Predicted dehydrogenases and related proteins Length = 334 Score = 302 bits (773), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 151/328 (46%), Positives = 215/328 (65%), Gaps = 8/328 (2%) Query: 3 IRFGVIGTNWITDRFIQAGLENEEFILTAVYSRTEEKGRDFAAKYAGASIYTNLEKMVAS 62 IRF VIGTNWIT +F++A E+ ++ LTAVYSR+ E+ + FA ++ ++T+LE M S Sbjct: 8 IRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFANDFSVEHLFTSLEAMAES 67 Query: 63 EEVDAVYIASPNSMHAEHALVCMNHGKHVICEKPAASNSAELRAMIEAARDNDVLFMEAM 122 + +DAVYIASPNS+H + ++H +VICEKP ASN AE+ A I AR+N V+ EA Sbjct: 68 DAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAEVDAAIACARENQVVLFEAF 127 Query: 123 KSTFMPNFGVIQDNLYKLGQVRRYFAGYCQYSSRYDAYRQGTVLNAFNPAFSNGSLMDLG 182 K+ +PNF +++ L K+G++R+ F YCQYSSRY Y G N FNPAFSNGS+MD+G Sbjct: 128 KTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYLDGENPNTFNPAFSNGSIMDIG 187 Query: 183 IYCLYPMVALFGKPESVRAVGLMLASGVDGEGSIVMRYPDMDAVVMHSKIADSYLPAEIQ 242 YCL VALFG+P+SV+A +LASGVD +G +VM Y D + HSK++DS L +EIQ Sbjct: 188 FYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMDYGDFSVTLQHSKVSDSVLASEIQ 247 Query: 243 GENGTMVIDKINQPYQVKIHYRDGTVEELTLPQVFEPMYYEVEEFIRLIRSGQRESSVNS 302 GE G++VI+K+++ +V R +++LT PQ M YE E F L+ + + Sbjct: 248 GEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHINTMLYEAELFATLV-----DEHLVD 302 Query: 303 H---ANSLAVAEVMEEARAQIGLRYASD 327 H A S A+++ E R Q G+ + +D Sbjct: 303 HPGLAVSRITAKLLTEIRRQTGVIFPAD 330 >ECs3969 [R] COG0673 Predicted dehydrogenases and related proteins Length = 334 Score = 302 bits (773), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 151/328 (46%), Positives = 215/328 (65%), Gaps = 8/328 (2%) Query: 3 IRFGVIGTNWITDRFIQAGLENEEFILTAVYSRTEEKGRDFAAKYAGASIYTNLEKMVAS 62 IRF VIGTNWIT +F++A E+ ++ LTAVYSR+ E+ + FA +++ ++T+LE M S Sbjct: 8 IRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFANEFSVEHLFTSLEAMAES 67 Query: 63 EEVDAVYIASPNSMHAEHALVCMNHGKHVICEKPAASNSAELRAMIEAARDNDVLFMEAM 122 + +DAVYIASPN++H + ++H HVICEKP ASN AE+ A I AR+N V+ EA Sbjct: 68 DAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAEVDAAIACARENQVVLFEAF 127 Query: 123 KSTFMPNFGVIQDNLYKLGQVRRYFAGYCQYSSRYDAYRQGTVLNAFNPAFSNGSLMDLG 182 K+ +PNF +++ L K+G++R+ F YCQYSSRY Y G N FNPAFSNGS+MD+G Sbjct: 128 KTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYLDGENPNTFNPAFSNGSIMDIG 187 Query: 183 IYCLYPMVALFGKPESVRAVGLMLASGVDGEGSIVMRYPDMDAVVMHSKIADSYLPAEIQ 242 YCL VALFG+P+SV+A +LASGVD G +VM Y D + HSK++DS L +EIQ Sbjct: 188 FYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMDYGDFSVTLQHSKVSDSVLASEIQ 247 Query: 243 GENGTMVIDKINQPYQVKIHYRDGTVEELTLPQVFEPMYYEVEEFIRLIRSGQRESSVNS 302 GE G++VI+K+++ +V R +++LT PQ M YE E F L+ + + Sbjct: 248 GEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHINTMLYEAELFATLV-----DEHLVD 302 Query: 303 H---ANSLAVAEVMEEARAQIGLRYASD 327 H A S A+++ E R Q G+ + +D Sbjct: 303 HPGLAVSRITAKLLTEIRRQTGVIFPAD 330 Score = 300 bits (768), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 151/328 (46%), Positives = 215/328 (65%), Gaps = 8/328 (2%) Query: 3 IRFGVIGTNWITDRFIQAGLENEEFILTAVYSRTEEKGRDFAAKYAGASIYTNLEKMVAS 62 IRF VIGTNWIT +F++A E+ ++ LTAVYSR+ E+ + FA ++ ++T+LE M S Sbjct: 8 IRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFANDFSVEHLFTSLEAMAES 67 Query: 63 EEVDAVYIASPNSMHAEHALVCMNHGKHVICEKPAASNSAELRAMIEAARDNDVLFMEAM 122 + +DAVYIASPNS+H + ++H +VICEKP ASN AE+ A I AR+N V+ EA Sbjct: 68 DAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAEVDAAIACARENQVVLFEAF 127 Query: 123 KSTFMPNFGVIQDNLYKLGQVRRYFAGYCQYSSRYDAYRQGTVLNAFNPAFSNGSLMDLG 182 K+ +PNF +++ L K+G++R+ F YCQYSSRY Y G N FNPAFSNGS+MD+G Sbjct: 128 KTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYLDGENPNTFNPAFSNGSIMDIG 187 Query: 183 IYCLYPMVALFGKPESVRAVGLMLASGVDGEGSIVMRYPDMDAVVMHSKIADSYLPAEIQ 242 YCL VALFG+P+SV+A +LASGVD +G +VM Y D + HSK++DS L +EIQ Sbjct: 188 FYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMDYGDFSVTLQHSKVSDSVLASEIQ 247 Query: 243 GENGTMVIDKINQPYQVKIHYRDGTVEELTLPQVFEPMYYEVEEFIRLIRSGQRESSVNS 302 GE G++VI+K+++ +V R +++LT PQ M YE E F L+ + + Sbjct: 248 GEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHINTMLYEAELFATLV-----DEHLVD 302 Query: 303 H---ANSLAVAEVMEEARAQIGLRYASD 327 H A S A+++ E R Q G+ + +D Sbjct: 303 HPGLAVSRITAKLLTEIRRQTGVIFPAD 330 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,623,099 Number of extensions: 989538 Number of successful extensions: 1880 Number of sequences better than 1.0e-05: 123 Number of HSP's gapped: 1854 Number of HSP's successfully gapped: 123 Length of query: 328 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 221 Effective length of database: 26,037,693 Effective search space: 5754330153 Effective search space used: 5754330153 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)