BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0006.p (262 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl888 [E] COG1177 ABC-type spermidine/putrescine transport syst... 210 2e-54 AGl1232 [E] COG1177 ABC-type spermidine/putrescine transport sys... 137 1e-32 SMa2203 [E] COG1177 ABC-type spermidine/putrescine transport sys... 132 4e-31 PA0606 [E] COG1177 ABC-type spermidine/putrescine transport syst... 128 1e-29 SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport sys... 126 4e-29 >AGl888 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 832 Score = 210 bits (535), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 18/250 (7%), Positives = 41/250 (16%) Query: 10 LYTLLVFIFLLGPLVIISITSFEPGTVLKFPPEGFSLRWYKNIFEVSAFMETFRTSIVIS 69 + L++ T +F + F + Sbjct: 9 RLAGFKTLLNDCQLLLGCPPPAANITRQQFNVSIVVRHKPVLKPVLEPFCLCRLSGRNGG 68 Query: 70 XXXXXXXXXXXXXXXXXXSRYRFRGRDTLNAVFLSPVLIPGIVLGFTLLRYLIVVYNLPI 129 + ++ D + L + Sbjct: 69 QFIGNILRRAEGGQLSLGWKEQWDNLDFSHITVGVLSQAYEYYLRKHAPKQQQQEGGFYT 128 Query: 130 VAGLLIGHTVIMLPFIIRVIASSLENFDFAVEEAAQSLGATRLFTFFKVVLPNIRSGILA 189 + + S+ A L A A Sbjct: 129 PHPIANLMVSAAFRGLNAQGISAAARILDPAAGAGVFLLAAFRELVAARWRSEGHRPDTA 188 Query: 190 AVLIAFLESFNNVDISVFMTGPGISTLPIQMLTYVQNYFDPTIAAISVILMVLTAALMFI 249 A+ + DI+ L + + N + V+ + Sbjct: 189 ALREILYKQVRGFDINEAALRFCALGLYLLSIELDPNPKPVDKLHFDDLRGVVLHRPVDT 248 Query: 250 IERLMGFAYF 259 + A Sbjct: 249 TDVDRPEAKQ 258 >AGl1232 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 134 Score = 137 bits (346), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 0/240 (0%), Positives = 0/240 (0%) Query: 12 TLLVFIFLLGPLVIISITSFEPGTVLKFPPEGFSLRWYKNIFEVSAFMETFRTSIVISXX 71 V + + ++ + + + +S Sbjct: 19 AASCEKNFTVSGVPMVTAVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQINKALSSI 78 Query: 72 XXXXXXXXXXXXXXXXSRYRFRGRDTLNAVFLSPVLIPGIVLGFTLLRYLIVVYNLPIVA 131 Sbjct: 79 138 Query: 132 GLLIGHTVIMLPFIIRVIASSLENFDFAVEEAAQSLGATRLFTFFKVVLPNIRSGILAAV 191 Sbjct: 139 198 Query: 192 LIAFLESFNNVDISVFMTGPGISTLPIQMLTYVQNYFDPTIAAISVILMVLTAALMFIIE 251 Sbjct: 199 258 >SMa2203 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 264 Score = 132 bits (333), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 78/249 (31%), Positives = 141/249 (56%), Gaps = 1/249 (0%) Query: 7 ALSLYTLLVFIFLLGPLVIISITSFEPGTVLKFPPEGFSLRWYKNIFEVSAFMETFRTSI 66 AL L T + FL+ P+ + I + P + PP GF+L W++ + F+ S+ Sbjct: 10 ALRLVTFSMMAFLIFPIAVTLIVAVNPREFV-LPPNGFTLDWFRAAWSSKTFLRGMGVSL 68 Query: 67 VISXXXXXXXXXXXXXXXXXXSRYRFRGRDTLNAVFLSPVLIPGIVLGFTLLRYLIVVYN 126 V+ +R F G+ +N + ++P+LIP +L L Y + V Sbjct: 69 VLGLAAALIANALALPAAIALARTNFPGKGAINLILMTPLLIPTTILSLALYIYFVRVGY 128 Query: 127 LPIVAGLLIGHTVIMLPFIIRVIASSLENFDFAVEEAAQSLGATRLFTFFKVVLPNIRSG 186 + LL+GH + ++P+ +R++ +SL NFD + EEAA+++GA R+ T F V LP IR+G Sbjct: 129 GSGLVPLLVGHAIHIMPYAVRILTASLLNFDPSYEEAARNVGAGRIRTLFSVTLPVIRTG 188 Query: 187 ILAAVLIAFLESFNNVDISVFMTGPGISTLPIQMLTYVQNYFDPTIAAISVILMVLTAAL 246 +++++ + F+ S+N+ ISVF+ P + LP+++ +Y++ +D AA++ L++L+A Sbjct: 189 LISSLTLCFILSWNDFPISVFLAPPSWTPLPVELYSYIKFQYDAVAAALASSLILLSAVA 248 Query: 247 MFIIERLMG 255 M +I+RL G Sbjct: 249 MVVIDRLAG 257 >PA0606 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 276 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 71/216 (32%), Positives = 126/216 (58%), Gaps = 10/216 (4%) Query: 30 SFEPGTVLKFPPEGFSLRWYKNIFEVSAFMETFRTSIVISXXXXXXXXXXXXXXXXXXSR 89 SF GT L +P +GFSLRWY + F+ + +M + S++++ +R Sbjct: 39 SFNSGTFLVYPLQGFSLRWYADFFQSAEWMRSLTNSMIVAPAATLLAMVFGTLAAIGLTR 98 Query: 90 YRFRGRDTLNAVFLSPVLIPGIVLGFTLLRYLIVVYNLPIVAG-----LLIGHTVIMLPF 144 FRG+ + ++ +SP+++P +++G + YL + P+ G L++ H V+ +PF Sbjct: 99 GEFRGKALVMSLMISPMVVPVVIIG--VASYL---FFAPLGLGNSYLSLIVVHAVLGVPF 153 Query: 145 IIRVIASSLENFDFAVEEAAQSLGATRLFTFFKVVLPNIRSGILAAVLIAFLESFNNVDI 204 +I ++++L+ F++ + AA SLGA L TFF+V LP I G+++ L AF SF+ V + Sbjct: 154 VIITVSATLQGFNYNLVRAAASLGAPPLTTFFRVTLPLIAPGVISGALFAFATSFDEVVV 213 Query: 205 SVFMTGPGISTLPIQMLTYVQNYFDPTIAAISVILM 240 ++F+ GP +TLP QM + ++ PTIAA + +L+ Sbjct: 214 TLFLAGPEQATLPRQMFSGIRENLSPTIAAAATLLI 249 >SMa0802 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 266 Score = 126 bits (316), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 68/215 (31%), Positives = 119/215 (55%) Query: 22 PLVIISITSFEPGTVLKFPPEGFSLRWYKNIFEVSAFMETFRTSIVISXXXXXXXXXXXX 81 P++ I++ SF L FPP G++LRWY+ + E ++++ TS I+ Sbjct: 29 PILFIAVLSFGSSQWLIFPPPGWTLRWYQELLEDPRWLDSAWTSFRIAIIVTVLSVLLGL 88 Query: 82 XXXXXXSRYRFRGRDTLNAVFLSPVLIPGIVLGFTLLRYLIVVYNLPIVAGLLIGHTVIM 141 +R RF R+ L A+FL+P+++P +VL L + + + + G +I H V+ Sbjct: 89 VTSFGLNRGRFLFREALKALFLTPMILPVVVLAVALYAFFLQIGLNGTLTGFVISHLVLA 148 Query: 142 LPFIIRVIASSLENFDFAVEEAAQSLGATRLFTFFKVVLPNIRSGILAAVLIAFLESFNN 201 LPF I I ++LE FD ++E+AA GA+ +V LP I G+ +A + +FL S++ Sbjct: 149 LPFSILSITNALEGFDKSIEDAAVLCGASPFEAKIRVTLPAISHGVFSAAIFSFLTSWDE 208 Query: 202 VDISVFMTGPGISTLPIQMLTYVQNYFDPTIAAIS 236 V +++FM P + TLP+++ + ++ P +AA S Sbjct: 209 VVVAIFMASPTLQTLPVKVWSTLRQDLTPVVAAAS 243 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.332 0.146 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,197,777 Number of extensions: 558555 Number of successful extensions: 2633 Number of sequences better than 1.0e-05: 185 Number of HSP's gapped: 2580 Number of HSP's successfully gapped: 198 Length of query: 262 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 158 Effective length of database: 26,383,695 Effective search space: 4168623810 Effective search space used: 4168623810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 115 (48.9 bits)