BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0005.p (272 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl889 [E] COG1176 ABC-type spermidine/putrescine transport syst... 149 6e-36 BMEI0413 [E] COG1176 ABC-type spermidine/putrescine transport sy... 134 2e-31 PA0303 [E] COG1176 ABC-type spermidine/putrescine transport syst... 126 3e-29 YPO1333 [E] COG1176 ABC-type spermidine/putrescine transport sys... 125 1e-28 SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport sys... 122 5e-28 >AGl889 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 832 Score = 149 bits (375), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 8/220 (3%), Positives = 30/220 (13%), Gaps = 1/220 (0%) Query: 22 IPITLTIASTFVQDGKLTLEGYLHFFRDGYFNRILLTTLRVSAVTTLVCMVLGYPVAYYI 81 S ++ L+ + Sbjct: 76 RRAEGGQLSLGWKEQWDNLDFSHITVGVLSQAYEYYLRKHAPKQQQQEGGFYTPHPIANL 135 Query: 82 SRTSVRKKSVLLALSIFPLLTSPVVRSFSWMIILGKKGLVNSLLVNTGIIDKPLDILYTP 141 ++ + +S + P + +++ ++ + D Sbjct: 136 MVSAAFRGLNAQGISAAARILDPAAGAGVFLLAAFRELVAARWRSEGHRPDTAALREILY 195 Query: 142 TAMMIGLVHLFLPLIIITLLGVMENLDHELVRAARSLGASPFTAFRKITFPXXXXXXXXX 201 + ++ L ++ + L Sbjct: 196 KQVRGFDINEAALRFCALGLYLLSIELDPNPKPVDKLHFDDLRGVVLHRPVDTTDVDRPE 255 Query: 202 XXXXXXXXXTAYTTPALLGG-KERVVSTFLYQNAMTLNDW 240 N Sbjct: 256 AKQLGSLGSLIGEEHDGQYDLVIGNPPWASATGLEDWNLL 295 >BMEI0413 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 303 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 67/213 (31%), Positives = 121/213 (56%), Gaps = 1/213 (0%) Query: 29 ASTFVQDGKLTLEGYLHFFRDGYFNRILLTTLRVSAVTTLVCMVLGYPVAYYISRTSVRK 88 A+ + + +L+ + YL D + + L+++R++A++T++ +++ YP+AY I+R Sbjct: 58 AANWEKIKQLSFDNYLWLLDDPLYYKAYLSSVRIAAISTVLTLIVAYPMAYGIARAPATI 117 Query: 89 KSVLLALSIFPLLTSPVVRSFSWMIILGKKGLVNSLLVNTGIIDKPLDILYTPTAMMIGL 148 + LL + I P TS ++R ++W+ IL +GL+N L+ IID PL+IL T TA+ IG+ Sbjct: 118 RPTLLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMGLNIIDTPLNILNTDTAVFIGI 177 Query: 149 VHLFLPLIIITLLGVMENLDHELVRAARSLGASPFTAFRKITFPXXXXXXXXXXXXXXXX 208 V+ +LP +++ + +E +D+ L+ AA+ LG +PF AF KITFP Sbjct: 178 VYSYLPFMVLPIYSSLEKMDYSLIEAAQDLGCTPFAAFWKITFPLSLAGVLAGCFLVFIP 237 Query: 209 XXTAYTTPALLGGKERV-VSTFLYQNAMTLNDW 240 + P LLGG + + + ++ + DW Sbjct: 238 AVGEFVIPDLLGGSQTLMIGKTIWSEFFSNRDW 270 >PA0303 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 293 Score = 126 bits (317), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 73/204 (35%), Positives = 113/204 (55%), Gaps = 1/204 (0%) Query: 38 LTLEGYLHFFRDGYFNRILLTTLRVSAVTTLVCMVLGYPVAYYISRTSVRKKSVLLALSI 97 L L YL D + L +L+++ ++TL+C+++GYP+AY I+R+S ++VLL L + Sbjct: 57 LNLGNYLMLTDDPLYIDAYLGSLKMAFISTLICLLVGYPMAYAIARSSQSSQTVLLLLIM 116 Query: 98 FPLLTSPVVRSFSWMIILGKKGLVNSLLVNTGIIDKPLDILYTPTAMMIGLVHLFLPLII 157 P T+ ++R ++WM IL GL+NS+L+ G+I +PL IL T A+ IG+V+ +LP +I Sbjct: 117 MPTWTAILIRVYAWMGILSNNGLLNSVLMGLGLISEPLTILNTNIAVYIGIVYSYLPFMI 176 Query: 158 ITLLGVMENLDHELVRAARSLGASPFTAFRKITFPXXXXXXXXXXXXXXXXXXTAYTTPA 217 + L + D L+ AA LGA FT+F KIT P + P Sbjct: 177 LPLYANLVKHDPSLLEAASDLGARNFTSFWKITVPLSKNGIIAGCMLVFIPAVGEFVIPE 236 Query: 218 LLGGKERV-VSTFLYQNAMTLNDW 240 LLGG E + + L+Q DW Sbjct: 237 LLGGPETLMIGKVLWQEFFNNRDW 260 >YPO1333 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 321 Score = 125 bits (313), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 66/185 (35%), Positives = 106/185 (57%), Gaps = 1/185 (0%) Query: 57 LTTLRVSAVTTLVCMVLGYPVAYYISRTSVRKKSVLLALSIFPLLTSPVVRSFSWMIILG 116 L +L+V+AV+TL C+++GYP+A+ I+ + +++LL L I P TS ++R ++WM IL Sbjct: 104 LQSLQVAAVSTLCCLLIGYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILK 163 Query: 117 KKGLVNSLLVNTGIIDKPLDILYTPTAMMIGLVHLFLPLIIITLLGVMENLDHELVRAAR 176 G++N+ L+ TGIID+PL IL+T A+ IG+V+ +LP +++ + + LD+ LV AA Sbjct: 164 NNGILNNFLIWTGIIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAAL 223 Query: 177 SLGASPFTAFRKITFPXXXXXXXXXXXXXXXXXXTAYTTPALLGGKERV-VSTFLYQNAM 235 LGA PF F + P + P LLGG + + + L+Q Sbjct: 224 DLGARPFKTFISVIIPLTKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFF 283 Query: 236 TLNDW 240 DW Sbjct: 284 NNRDW 288 >SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 296 Score = 122 bits (307), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 72/220 (32%), Positives = 121/220 (55%), Gaps = 6/220 (2%) Query: 23 PITLTIASTFVQDGKLTLEGYLHFFRDGYFNRILLTTLRVSAVTTLVCMVLGYPVAYYIS 82 P+ IA +F T Y F + +L +T+ V+ V T+ ++LG+PVAY ++ Sbjct: 46 PVARLIALSFSGG---TFSHYRRIFTEPLHLEVLFSTIEVAFVVTVAGLLLGFPVAYLMA 102 Query: 83 RTSVRKKSVLLALSIF-PLLTSPVVRSFSWMIILGKKGLVNSLLVNTGIIDKPLDILYTP 141 R S R ++ +A +F PL TS ++RS++W+++L + G+VN LL +TG+ + PL ++YT Sbjct: 103 RLS-RGLAMAVAACVFVPLWTSVLIRSYAWVVLLQRNGIVNKLLADTGVTEGPLKLIYTQ 161 Query: 142 TAMMIGLVHLFLPLIIITLLGVMENLDHELVRAARSLGASPFTAFRKITFPXXXXXXXXX 201 A+++ + H+ +P +I+ + + L + VRAAR+LGA P AF +T P Sbjct: 162 GAVILAMTHVLMPFMILPIYSALRALPPDYVRAARNLGAGPIRAFVTVTLPLSLPGIFAG 221 Query: 202 XXXXXXXXXTAYTTPALLGGKER-VVSTFLYQNAMTLNDW 240 Y TPAL+GG +++T + Q L DW Sbjct: 222 SVMCFVLALGFYITPALVGGPSSMLMATLIGQQTTVLLDW 261 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.140 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,953,253 Number of extensions: 572869 Number of successful extensions: 2021 Number of sequences better than 1.0e-05: 115 Number of HSP's gapped: 2014 Number of HSP's successfully gapped: 115 Length of query: 272 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 167 Effective length of database: 26,268,361 Effective search space: 4386816287 Effective search space used: 4386816287 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)