BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0002.p (167 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl890 [E] COG0687 Spermidine/putrescine-binding periplasmic pro... 80 1e-15 SMa2209 [E] COG0687 Spermidine/putrescine-binding periplasmic pr... 52 3e-07 mll1024 [E] COG0687 Spermidine/putrescine-binding periplasmic pr... 49 5e-06 >AGl890 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Length = 832 Score = 80.5 bits (197), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 8/94 (8%), Positives = 19/94 (20%), Gaps = 2/94 (2%) Query: 53 LVISTWGFSEDFFKESVYAPFEKEHNVKIVVEIGNNAERLNKIRQGSSD--VDVVYLSDY 110 + F S+ + + N + + Sbjct: 26 CPPPAANITRQQFNVSIVVRHKPVLKPVLEPFCLCRLSGRNGGQFIGNILRRAEGGQLSL 85 Query: 111 YAQQAINEGLFETIDRSKIPNLDKIYDIAKAPLG 144 ++ + F I + + Y AP Sbjct: 86 GWKEQWDNLDFSHITVGVLSQAYEYYLRKHAPKQ 119 >SMa2209 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Length = 340 Score = 52.4 bits (124), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 30/84 (35%), Positives = 47/84 (55%), Gaps = 2/84 (2%) Query: 53 LVISTWGFSEDFFKESVYAPFEKEHNVKIVVEIGNNAERLNKIRQGSSDVDVVYLSDYYA 112 L ++TWG S ++V FE+EHN +I +G + + L K R G DV L + A Sbjct: 27 LRVTTWGGSYQATYQAVAQKFEEEHNCRIEWVVGASPDHLIKARLGQVDVVTNTLLNSIA 86 Query: 113 QQAINEGLFETIDRSKIPNLDKIY 136 + EGL++ +D +KIPN+ +Y Sbjct: 87 GE--KEGLWQKLDPAKIPNMANLY 108 >mll1024 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Length = 182 Score = 48.5 bits (114), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 4/77 (5%), Positives = 11/77 (14%), Gaps = 1/77 (1%) Query: 63 DFFKESVYAPFEKEHNVKIVVEIGNNAERL-NKIRQGSSDVDVVYLSDYYAQQAINEGLF 121 + + Q + + + + Sbjct: 36 SSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPP 95 Query: 122 ETIDRSKIPNLDKIYDI 138 E+I I Sbjct: 96 ESIYPDFRYRATDPSGI 112 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,411,650 Number of extensions: 275416 Number of successful extensions: 568 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 583 Number of HSP's successfully gapped: 3 Length of query: 167 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 68 Effective length of database: 26,960,365 Effective search space: 1833304820 Effective search space used: 1833304820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)