BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0004.p (424 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0367 [GEPR] COG0477 Permeases of the major facilitator superfa... 140 5e-33 SPy1392 [GEPR] COG0477 Permeases of the major facilitator superf... 106 8e-23 SP1587 [GEPR] COG0477 Permeases of the major facilitator superfa... 82 2e-15 NMB1362 [GEPR] COG0477 Permeases of the major facilitator superf... 68 2e-11 NMA1574 [GEPR] COG0477 Permeases of the major facilitator superf... 68 3e-11 >AF0367 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 397 Score = 140 bits (352), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 100/390 (25%), Positives = 164/390 (42%), Gaps = 17/390 (4%) Query: 7 SKRWLIVLGTVIMQMGLGTIYTWSLFNAQLVSKFGWELXXXXXXXXXXXXXXXXXXXXXG 66 + +WL++ ++ + LG +Y WS+F L++ FGW G Sbjct: 2 ASKWLVIFAGFLLNLMLGIVYAWSVFVNPLMNTFGWSKTIASLPFSIFLLIFALMMVPAG 61 Query: 67 KLQDRLGLRRLTAAAGIMLGLGLIFSSQARSLPMLYLLA---GVVVGYADGTAYITSLSN 123 + QDR+G R++ G++LG+G + S S+ Y L GV+ G G Y + Sbjct: 62 RTQDRIGPRKVAMLGGVLLGVGFLLSGLIESIQSPYWLIFSYGVIAGAGCGLGYACPIPV 121 Query: 124 LIKWFPKNKGLISGVSVGAYGTGSLLFKYINGSLIDSAGVSNAFLYWGLMVMAMIVIGSM 183 KWFP+ GL +G+ V +G +L+F + LID+ G+S F G++++ + V + Sbjct: 122 ARKWFPERVGLATGLVVMGFGMSALIFAPLERILIDTYGISTTFYILGVILLIVAVFAAS 181 Query: 184 LVREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDYTVKEMLRTKEAYLLFVVFFTACM 243 L+ + EMLR Y+L++ FF + Sbjct: 182 LLSN---------PPELPAVQASNPAKVEVVTGKPELGPSEMLRDYRFYVLWLSFFFMAL 232 Query: 244 SGLYLIGVVKDIGVQLXXXXXXXXXXXXXXXXXXXTAGRLILGALSDRMSRLKXXXXXXX 303 +GL +IG + Q GR GALSD++ R Sbjct: 233 AGLMVIGHIAPYA-QERGLEPLAAAFAVSILSVANAVGRPGAGALSDKIGRAMTMFVLFL 291 Query: 304 XXXXXXXXXXYATLSYGLFFTCVAAIAFCFGGNITVFPAIVGDFFGLANHSKNYGIVYQG 363 + L+ + C A I F +G N ++FP+ GDFFG N NYG+V+ Sbjct: 292 IQGITLIAFPHVALTLITIYICAAIIGFNYGANFSLFPSATGDFFGTKNLGVNYGLVFTS 351 Query: 364 FGIGALSGSFVAAFL----GGFKPTFTVIG 389 +G+G L G +A ++ G ++ F V G Sbjct: 352 YGVGGLVGPIMAGYVFDVTGSYEIAFLVAG 381 >SPy1392 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 106 bits (264), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 86/371 (23%), Positives = 151/371 (40%), Gaps = 13/371 (3%) Query: 7 SKRWLIVLGTVIMQMGLGTIYTWSLFNAQLVSKFGWELXXXXXXXXXXXXXXXXXXXXXG 66 +KR++I +++ + LG+ Y WS++ ++ + GW+ G Sbjct: 4 TKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPVAFAFSLAIFCLGLSAAFMG 63 Query: 67 KLQDRLGLRRLTAAAGIMLGLGLIFSSQA---RSLPMLYLLAGVVVGYADGTAYITSLSN 123 L ++ G R + I+ G + + A + + +LY+ GV+ G G YIT +S Sbjct: 64 NLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLYIGYGVIGGLGLGAGYITPIST 123 Query: 124 LIKWFPKNKGLISGVSVGAYGTGSLLFKYINGSLIDSAGVSNAFLYWGLMVMAMIVIGSM 183 +IKWFP +G+ +G ++ +G SLL I LI++ G+ F GL+ + +++ S Sbjct: 124 IIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIETEGLVATFYLLGLIYLIVMLFASQ 183 Query: 184 LVREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDYTVKEMLRTKEAYLLFVVFFTACM 243 L+ + + T KE L+TK Y L+V+ F Sbjct: 184 LIIK---------PTAAEIAILDKKRLQNNSYLIEGMTAKEALKTKSFYCLWVILFINIT 234 Query: 244 SGLYLIGVVKDIGVQLXXXXXXXXXXXXXXXXXXXTAGRLILGALSDRMSRLKXXXXXXX 303 GL LI VV + L GRL+ +LSD + R Sbjct: 235 CGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVWASLSDYIGRRVTVILLFL 294 Query: 304 XXXXXXXXXXYATLSYGLFFTCVAAIAFCFGGNITVFPAIVGDFFGLANHSKNYGIVYQG 363 +A S +F +A + C+G ++ P + D FG + +G + Sbjct: 295 VSIIMTISLIFAHSSL-IFMISIATLMTCYGAGFSLIPPYLSDLFGAKELATLHGYILTA 353 Query: 364 FGIGALSGSFV 374 + I AL+G + Sbjct: 354 WAIAALTGPML 364 >SP1587 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 408 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 80/373 (21%), Positives = 146/373 (39%), Gaps = 13/373 (3%) Query: 7 SKRWLIVLGTVIMQMGLGTIYTWSLFNAQLVSKFGWELXXXXXXXXXXXXXXXXXXXXXG 66 S R++I VI+ + LG+ Y WS++ ++ K GW+ G Sbjct: 3 SNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVAFAFSLAIFCLGLSAAFMG 62 Query: 67 KLQDRLGLRRLTAAAGIMLGLGLI---FSSQARSLPMLYLLAGVVVGYADGTAYITSLSN 123 +L ++ G + + + + + G I F+ + L +LYL G++ G G YIT +S Sbjct: 63 RLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYLAYGILGGLGLGAGYITPVST 122 Query: 124 LIKWFPKNKGLISGVSVGAYGTGSLLFKYINGSLIDSAGVSNAFLYWGLMVMAMIVIGSM 183 +IKWFP +GL +G+++ +G SLL I LI G+ F G ++++ S Sbjct: 123 IIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGVGLVETFYILGASYFIIMLLASQ 182 Query: 184 LVREXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKDYTVKEMLRTKEAYLLFVVFFTACM 243 ++ + + L++ Y+L+++FF Sbjct: 183 FIKR---------PNEQELAILSSSGKEKTASLTQGMAANQALKSNRFYMLWIIFFINIA 233 Query: 244 SGLYLIGVVKDIGVQLXXXXXXXXXXXXXXXXXXXTAGRLILGALSDRMSRLKXXXXXXX 303 GL LI + ++ GRL+ +LSD + R Sbjct: 234 CGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWASLSDYIGRPLTFSILLL 293 Query: 304 XXXXXXXXXXYATLSYGLFFTCVAAIAFCFGGNITVFPAIVGDFFGLANHSKNYGIVYQG 363 T S LF ++ + C+G ++ PA + D FG + +G + Sbjct: 294 VNLFFSLSLWLFTDSV-LFVVAMSILMTCYGAGFSLIPAYLSDIFGTKELAALHGYILTA 352 Query: 364 FGIGALSGSFVAA 376 + + L+G + A Sbjct: 353 WAMAGLAGPILLA 365 >NMB1362 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 513 Score = 68.2 bits (165), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 56/198 (28%), Positives = 94/198 (47%), Gaps = 17/198 (8%) Query: 6 GSKRWLIVLGTVIMQMGLGTIYTWSLFNAQLVSKFG--------WELXXXXXXXXXXXXX 57 G RWL+ + + + +G IY +S+FNA L G W+L Sbjct: 14 GFNRWLVPPAALAVHLAIGQIYAYSVFNAPLTKLIGITESAAGDWKLTTVGWIFSIALAM 73 Query: 58 XXXXXXXXGKLQDRLGLRRLTAAAGIMLGLGLIFSS---QARSLPMLYLLAGVVVGYADG 114 G +R+G R+ AA LG S+ + +L +LYL GV+ G G Sbjct: 74 LGASAALFGTWMERVGPRKAIFAAACCFSLGFFVSAFGVRTHNLFLLYLGNGVIGGVGLG 133 Query: 115 TAYITSLSNLIKWFPKNKGLISGVSVGAYGTGSLLFKYINGSLID------SAGVSNAFL 168 YI +S L+KWFP G+ +G+++ +G G++L ++ SL++ S GV+ F Sbjct: 134 LGYIGPVSTLMKWFPDKPGMATGLAIMGFGGGAMLASPLSVSLMNTFSNAASVGVAETFA 193 Query: 169 YWGLMVMAMIVIGSMLVR 186 GL+ +A+++ G+ +R Sbjct: 194 VLGLLYLALMMFGAFTIR 211 >NMA1574 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 209 Score = 68.2 bits (165), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 0/198 (0%), Positives = 0/198 (0%), Gaps = 17/198 (8%) Query: 6 GSKRWLIVLGTVIMQMGLGTIYTWSLFNAQLVSKFG--------WELXXXXXXXXXXXXX 57 + V+ Sbjct: 14 LPPLKYGYADLEPYISEEQLRIHHDKHHQGYVNNTNALLEMMDKARKEDTDFDYKATAKA 73 Query: 58 XXXXXXXXGKLQDRLGLRRLTAAAGIMLGLGLIFSS---QARSLPMLYLLAGVVVGYADG 114 + A + + V Sbjct: 74 LSFNLSGHVLHDFFWWEMTPASNASKEPVGEFAEAIKEDFGSFERFKKEFSKVASSVEGS 133 Query: 115 TAYITSLSNLIKWFPKNKGLISGVSVGAYGTGSLLFKYINGSLID------SAGVSNAFL 168 + K + V++ + + + + Sbjct: 134 GWAALTFCKGTKRLGIVQIEKHNVNLVPDFPIIMDLDVWEHAYYIDYKNDRGKFIEGFWN 193 Query: 169 YWGLMVMAMIVIGSMLVR 186 Sbjct: 194 211 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.142 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,918,419 Number of extensions: 820141 Number of successful extensions: 3051 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 3037 Number of HSP's successfully gapped: 12 Length of query: 424 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 315 Effective length of database: 25,807,025 Effective search space: 8129212875 Effective search space used: 8129212875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)