BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0007.p (493 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 209 1e-53 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 202 1e-51 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 159 9e-39 CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator supe... 145 2e-34 RP157 [GEPR] COG0477 Permeases of the major facilitator superfamily 144 4e-34 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 209 bits (531), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 121/396 (30%), Positives = 206/396 (52%), Gaps = 2/396 (0%) Query: 12 WLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGVIAPVVGYF 71 W+++ AV FG+F IL+ + +N A P M F A+ W+IT +++A G+ P+ GY Sbjct: 8 WMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTGYL 67 Query: 72 GDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTMTMIYQFIE 131 GDK K Y+ L F L S L ++W++ SL++FR LQG+ GG+++P +MT+I+ Sbjct: 68 GDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLIFDAFP 127 Query: 132 KEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAVALKCLPFQ 191 + ++ GPTLGG++ W+ LF++N+P G++ I A+ LP Sbjct: 128 RNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIYLPKI 187 Query: 192 RNAGETKSFDLPGFVTVILSSAFIILAFNKGNSW-GWTSWKXXXXXXXXXXXXMYFIRRE 250 +G+ K D GF+ V I+LAF + + T W F++ E Sbjct: 188 ARSGQIK-LDRWGFLFVTAGVGSILLAFGRMSDLTHLTEWINGVLLLLGALCLYVFVQVE 246 Query: 251 LSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPLTTALVLLP 310 ++PLL+L +FR ++ S+ + I ++ L++G FL+P+ LQ + P+ T LV LP Sbjct: 247 KRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPIMTGLVFLP 306 Query: 311 GSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLAWLMAVRNI 370 ++ + + G++ K GP T+ L +L L T + MA+R + Sbjct: 307 SALFTGLTMSIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIFAWMAIRGV 366 Query: 371 GIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQAT 406 G+ L MP T GM+++P+ + S++ N +RQ + Sbjct: 367 GMGLTTMPATTTGMNAIPEGLISRGSAMNNVLRQMS 402 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 202 bits (514), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 113/396 (28%), Positives = 201/396 (50%), Gaps = 5/396 (1%) Query: 14 IILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGVIAPVVGYFGD 73 I++ + G F+ IL+ T +NVA P MKDF QW+ TG++L GV+ P+ + + Sbjct: 13 ILIVMLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIE 72 Query: 74 KWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTMTMIYQFIEKE 133 +++ + LY+FA+ F + + + A L+ R++Q + G+++P ++ E Sbjct: 73 RFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPME 132 Query: 134 KQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAVALKCLPFQRN 193 ++ GPTL GW+ + W +LF I +P I+ I VA+ L +N Sbjct: 133 RRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLL---KN 189 Query: 194 AGETK--SFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXXXXMYFIRREL 251 G+ D+ G + + ++L F+ W +WK FIR + Sbjct: 190 VGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFIRYQS 249 Query: 252 SLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPLTTALVLLPG 311 S K PLLN KVF+ F + I+ + + L+ G L+P+FLQ ++ +PL + LVLLPG Sbjct: 250 SNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPG 309 Query: 312 SIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLAWLMAVRNIG 371 ++ A++SPV G ++ + G + ST+ ++L +TT + + +R+ G Sbjct: 310 ALVTAILSPVTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAG 369 Query: 372 IALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATG 407 +A+ MP+ A ++S+P ++ H S++ N +RQ G Sbjct: 370 MAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAG 405 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 159 bits (402), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 118/463 (25%), Positives = 208/463 (44%), Gaps = 12/463 (2%) Query: 14 IILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGVIAPVVGYFGD 73 I++++ F+ + S T +N+A MK+ + TVQW+ TGYLL G++ PV G Sbjct: 18 IMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQ 77 Query: 74 KWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTMTMIYQFIEKE 133 ++ + L+ +L L + + +A S L+ RI+Q + GL++P I Sbjct: 78 WFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPH 137 Query: 134 KQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAVALKCLPFQRN 193 K+ GPT G + + W +F I+LP ++A+ + + +N Sbjct: 138 KRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIA---YMQN 194 Query: 194 AGETKSFDLPGFVTVILSS---AFIILAF-NKGN-SWGWTSWKXXXXXXXXXXXXMYFIR 248 ET + +++ILS+ I+ F N G S GW+S + F Sbjct: 195 VSETTKPKI-DVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSI 253 Query: 249 RELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPLTTALVL 308 R+L++K+P++NL+ F+ F +I+ I + + S L+P++LQ T + LVL Sbjct: 254 RQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVL 313 Query: 309 LPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLAWLMAVR 368 LPG I MSPV G+L+ K GP W W S++T +T V + L Sbjct: 314 LPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCL 373 Query: 369 NIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQATGXXXXXXXXXXXXXXXXTHQKE 428 IGI++ MP G++ +P++ +++ N ++Q G + Sbjct: 374 MIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMST 433 Query: 429 LXXXXXXXXXLIKAQGMTMGVQDVFLTATVIGILAIPLTFLLR 471 + + Q +T GVQ F+ A ++ I+ + F ++ Sbjct: 434 VKNPADPA---VIPQALTAGVQHAFVFAMIVAIIGLIGAFFMK 473 >CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 458 Score = 145 bits (366), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 101/402 (25%), Positives = 178/402 (44%), Gaps = 5/402 (1%) Query: 3 NHTARNSSFWLIILAVFFGNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATG 62 N + F I++A F G ++ +T +N+A P F + W+ T Y LA Sbjct: 2 NERKKYIGFIGILIAAFLG----VIDSTIVNIALPNITDYFKVSLNDTSWISTIYALALA 57 Query: 63 VIAPVVGYFGDKWSYKYLYVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTT 122 V F D++ K L + + F + S LC I+ S+ L+I R +QG+ G +I P Sbjct: 58 VFMITAAKFADQFGRKKLMIIGILIFGVSSALCGISNSLLFLIIMRFVQGIGGAIITPIA 117 Query: 123 MTMIYQFIEKEKQXXXXXXXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIA 182 + M + KE+ GP LGG L Y W+S+F +N+P I A+ Sbjct: 118 LPMGIEIFGKERMNIVVGASGAVVGLAAASGPPLGGVLIKYINWQSVFFVNVPFAIAALI 177 Query: 183 VALKCLPFQRNAGETKSFDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXXX 242 + + C+ + +K D G +T+ +S +I A KG +GWTS Sbjct: 178 LIVICVNESYDNTVSKDIDWFGVMTLTISLFCLIFALLKGKDYGWTSTLIISLFLIFVVS 237 Query: 243 XMYFIRRELSLKEPLLNLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPL 302 + FI EL + P++ + +F++ FT S I I L ++ +LQ++ Q L Sbjct: 238 LILFIIIELKVSSPMIEINLFKEPTFTASSICYMITGFGLMCPLLILNYYLQNVLQYDTL 297 Query: 303 TTALVLLPGSIAMAVMSPVVGKLYSKVGPFWXXXXXXXXXXXSTWELSHLTLATTHVQLA 362 A +++ S+ +P+ L KV P + LS+L+ + + Sbjct: 298 EAAFIMMSVSLTGMFSTPIGSILGKKVSPRIINFIGMLSLGIGIFRLSYLSYGMSKGTMI 357 Query: 363 WLMAVRNIGIALAFMPVTNAGMSSVPKQITGHASSVTNWVRQ 404 + + + IG+ + ++++ + +PK+ +G S + N RQ Sbjct: 358 FDLIICGIGLGFSVQALSSS-VKFIPKEKSGIGSGIVNAARQ 398 >RP157 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 519 Score = 144 bits (362), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 101/390 (25%), Positives = 167/390 (42%), Gaps = 3/390 (0%) Query: 21 GNFMAILSTTTINVAFPVFMKDFHAEIGTVQWMITGYLLATGVIAPVVGYFGDKWSYKYL 80 G FM++L + + V A + W+ T YL+A +I P+ G+ S + Sbjct: 25 GMFMSVLDIQIVASSLSVIAAGLAASSDELSWVQTSYLIAEVIIIPITGFLARLLSTRIC 84 Query: 81 YVFALSGFTLFSGLCMIAWSIHSLVIFRILQGVFGGLIIPTTMTMIYQFIEKEKQXXXXX 140 Y A GFT+ S LC +A +I S++IFR LQG FGG +IP + +Y ++ Sbjct: 85 YFIAALGFTIMSVLCSLANNIESMIIFRALQGFFGGAMIPLVFSTVYIIFPASQRPTVTI 144 Query: 141 XXXXXXXXXXXXGPTLGGWLTGYFGWESLFMINLPIGIIAIAVALKCLPFQR-NAGETKS 199 GPTLGG++T W +F++N+ GI V + F + N K+ Sbjct: 145 LIGLVVTVAPTLGPTLGGYITEILSWHFMFLLNVIPGIFVCTVVFLYVNFDKPNYQLLKN 204 Query: 200 FDLPGFVTVILSSAFIILAFNKGNSWGWTSWKXXXXXXXXXXX-XMYFIRRELSLKEPLL 258 FD G + + L+ + +GN GW + I REL+ P+L Sbjct: 205 FDFLGILLMSLTLGLLQYVLEEGNKEGWLDDNLILFLSITVALGFILLIIRELTFINPIL 264 Query: 259 NLKVFRQNRFTYSLIINCIITVALYSGTFLIPVFLQDIQQSTPLTTALVLLPGSIAMAVM 318 +LK F FT+ I + ++ + LY +++P+FL I + L ++ +A + Sbjct: 265 DLKTFLYKDFTFGCIYSFVMGIGLYGAVYILPLFLFTIAEYDTLQIGATMMVTGVAQFLS 324 Query: 319 SPVVGKLYSKVGPFWXXXXXXXXXXXSTWEL-SHLTLATTHVQLAWLMAVRNIGIALAFM 377 +P+ G++ L S LT + VR + F+ Sbjct: 325 APLAGRMLGLGIDLRMMLTIGLGGFALGCHLNSCLTPDSKFAAFILPQFVRGFSLMFCFI 384 Query: 378 PVTNAGMSSVPKQITGHASSVTNWVRQATG 407 P N + ++PK+ G+AS + N R G Sbjct: 385 PTNNIALGNMPKEKVGNASGLYNLTRNLGG 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,590,801 Number of extensions: 1073002 Number of successful extensions: 4142 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 4055 Number of HSP's successfully gapped: 62 Length of query: 493 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 383 Effective length of database: 25,691,691 Effective search space: 9839917653 Effective search space used: 9839917653 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 118 (50.1 bits)