BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0012.p (294 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0057 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) 336 3e-92 ML0363 [S] COG4842 Uncharacterized protein conserved in bacteria 286 2e-77 SA0451 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) 284 1e-76 Rv1010 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) 169 5e-42 MT1039 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) 169 5e-42 >BH0057 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) Length = 289 Score = 336 bits (861), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 168/282 (59%), Positives = 211/282 (74%) Query: 4 NNNISSPARTKEIIQRYGFSFKKSLGQNFLIDQNILDKIVXXXXXXXXXXXXEIGPGIGA 63 +I++P RTKEI+ ++GF+FKKSLGQNFLID N+L IV EIGPGIGA Sbjct: 2 TKDIATPIRTKEILAKHGFTFKKSLGQNFLIDPNVLRNIVDVASLTPQSGAIEIGPGIGA 61 Query: 64 LTERLAATAGAVTAVEIDRRLIPILRDVLASYPHVTIRNDDVLKVNLQELFAEDFAAVER 123 LTE+LA A V A EID+RLIP+LR+ LA Y +VT+ N+DVLK +++++ A F + Sbjct: 62 LTEQLARQAKRVVAFEIDQRLIPVLRETLAPYENVTVINEDVLKADVKQVIATTFEEGQD 121 Query: 124 VSVVANLPYYVTTPILMKLLEEKLPLDNIVVMIQKEVAERMAASPGGKEYGSLSIAVQYY 183 + VVANLPYYVTTPILMKLLE KLP+ IVVMIQ EVA+R+AA PG KEYGSLSIA QYY Sbjct: 122 LMVVANLPYYVTTPILMKLLEAKLPVRGIVVMIQAEVADRIAAKPGTKEYGSLSIAAQYY 181 Query: 184 SEPELVCIVPRTVFIPQPNVESAVIRLKVRERPPVEVADEKHFFEVVQASFTQRRKTIAN 243 + EL VP+TVF+PQPNV+SAV+RL +RE+PPV VADE+ F +V+ASF RRKTI N Sbjct: 182 ATAELAVKVPKTVFVPQPNVDSAVLRLTIREKPPVTVADEEWLFAIVRASFANRRKTILN 241 Query: 244 NLKARFFPEEGRERLESLLAAAGIEPSRRGETLSLEEYARLS 285 NL +E +++ LA AGI+PSRRGETL++EE+A LS Sbjct: 242 NLIHNLVGKERKDKAVQALAEAGIDPSRRGETLTIEEFANLS 283 >ML0363 [S] COG4842 Uncharacterized protein conserved in bacteria Length = 294 Score = 286 bits (733), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 151/291 (51%), Positives = 197/291 (67%), Gaps = 3/291 (1%) Query: 1 MSGNN--NISSPARTKEIIQRYGFSFKKSLGQNFLIDQNILDKIVXXXXXXXXXXXXEIG 58 M+ NN IS+ RT++I++R+ F+FKK GQNFL D NIL KI EIG Sbjct: 1 MTENNIDRISNIIRTQDILRRHDFNFKKKFGQNFLTDHNILTKITQTAELSKEVNVIEIG 60 Query: 59 PGIGALTERLAATAGAVTAVEIDRRLIPILRDVLASYPHVTIRNDDVLKVNLQELFAEDF 118 PGIG+LT+ L A V A EID+ LIPIL + +A Y + T+ + D+LKV+L + Sbjct: 61 PGIGSLTQYLLEEAAEVMAFEIDKSLIPILEETMAPYDNFTLVSADILKVDLLSEIQKFK 120 Query: 119 AAVERVSVVANLPYYVTTPILMKLLEEKLPLDNIVVMIQKEVAERMAASPGGKEYGSLSI 178 + VVANLPYY+TTPILM L+E K+P VVM+QKEVA+R+AASP K YGSLSI Sbjct: 121 NPNLPIKVVANLPYYITTPILMHLIESKIPFSEFVVMMQKEVADRIAASPKTKAYGSLSI 180 Query: 179 AVQYYSEPELVCIVPRTVFIPQPNVESAVIRLKVRERPPVEVADEKHFFEVVQASFTQRR 238 AVQYY E + IVPRTVFIP PNV+SA++++ RE P VEV DE+ FF+ + +SF RR Sbjct: 181 AVQYYMEASVAFIVPRTVFIPAPNVDSAILKMVRREAPLVEVEDEEWFFKTMHSSFVHRR 240 Query: 239 KTIANNLKARFFPEEGRERLESLLAAAGIEPSRRGETLSLEEYARLSAVLL 289 KT+ NNL+A F +E + +E LLA A I P+ RGE LS+EE+A+L+ LL Sbjct: 241 KTLMNNLQAA-FGKESKPEIEKLLAQAEISPTIRGEALSIEEFAKLADALL 290 >SA0451 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) Length = 297 Score = 284 bits (727), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 143/287 (49%), Positives = 202/287 (70%), Gaps = 5/287 (1%) Query: 1 MSGNNNISSPARTKEIIQRYGFSFKKSLGQNFLIDQNILDKIVXXXXXXXXXXXXEIGPG 60 M N +I++P+RT+ ++ +YGF+FKKSLGQNFLID NI++ I+ EIGPG Sbjct: 1 MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG 60 Query: 61 IGALTERLAATAGAVTAVEIDRRLIPILRDVLASYPHVTIRNDDVLKVNLQELFAEDFAA 120 +G+LTE+LA A V A EID+RLIP+L D L+ Y +VT+ N+D+LK N++E Sbjct: 61 MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD 120 Query: 121 VERVSVVANLPYYVTTPILMKLLEEKLPLDNIVVMIQKEVAERMAASPGGKEYGSLSIAV 180 E++ VVANLPYY+TTPIL+ L+++ +P+D VVM+QKEV ER+ A G K YGSLSI V Sbjct: 121 CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV 180 Query: 181 QYYSEPELVCIVPRTVFIPQPNVESAVIRLKVRERPPVEVADEKHFFEVVQASFTQRRKT 240 QYY+E V VP++VF+P PNV+S V++L R P V V +E+ FF++ +A+F QRRKT Sbjct: 181 QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT 240 Query: 241 IANNLKARFFPEEGRERLE---SLLAAAGIEPSRRGETLSLEEYARL 284 I NN + F ++G++ E L AGI+P RRGETLS++++A+L Sbjct: 241 INNNYQNYF--KDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKL 285 >Rv1010 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) Length = 317 Score = 169 bits (428), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 105/273 (38%), Positives = 149/273 (54%), Gaps = 6/273 (2%) Query: 14 KEIIQRYGFSFKKSLGQNFLIDQNILDKIVXXXXXXXXXXXXEIGPGIGALTERLAATAG 73 + + + F +KSLGQNF+ D N + ++V E+GPG+G+LT L Sbjct: 20 RRLAKELDFRPRKSLGQNFVHDANTVRRVVAASGVSRSDLVLEVGPGLGSLTLALLDRGA 79 Query: 74 AVTAVEIDRRLIPILRDVLASYPHVTIRNDDVLKVNLQELFAEDFAAVERVSVVANLPYY 133 VTAVEID L L+ +A + H + V+ ++ L ED AA +VVANLPY Sbjct: 80 TVTAVEIDPLLASRLQQTVAEHSHSEVHRLTVVNRDVLALRREDLAAAP-TAVVANLPYN 138 Query: 134 VTTPILMKLLEEKLPLDNIVVMIQKEVAERMAASPGGKEYGSLSIAVQYYSEPELVCIVP 193 V P L+ LL E + + VM+Q EVAER+AA PG KEYG S+ ++++ +V Sbjct: 139 VAVPALLHLLVEFPSIRVVTVMVQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVS 198 Query: 194 RTVFIPQPNVESAVIRLKVRERPPVEVAD--EKHFFEVVQASFTQRRKTIANNLKARFFP 251 TVF P P V S ++R+ E P D + FE+V +F QRRKT N + Sbjct: 199 PTVFWPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIAFAQRRKTSRNAFVQ--WA 256 Query: 252 EEGRERLESLLAAAGIEPSRRGETLSLEEYARL 284 G E LLAA+ I+P+RRGETLS++++ RL Sbjct: 257 GSGSESANRLLAAS-IDPARRGETLSIDDFVRL 288 >MT1039 [J] COG0030 Dimethyladenosine transferase (rRNA methylation) Length = 432 Score = 169 bits (428), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 23/273 (8%), Positives = 55/273 (20%), Gaps = 6/273 (2%) Query: 14 KEIIQRYGFSFKKSLGQNFLIDQNILDKIVXXXXXXXXXXXXEIGPGIGALTERLAATAG 73 + + + + + + A A A Sbjct: 20 TVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVTKAVAAVNGPIAQAL 79 Query: 74 AVTAVEIDRRLIPILRDVLASYPHVTIRNDDVLKVNLQELFAEDFAAVERVSVVANLPYY 133 + + I+ D+ + + +L V+L A A Sbjct: 80 IGKDAKDQAGIDKIMIDLDGTENKSKFGANAILAVSLANAKAAAAAKGM-PLYEHIAELN 138 Query: 134 VTTPILMKLLEEKLPLDNIVVMIQKEVAERMAASPGGKEYGSLSIAVQYYSEPELVCIVP 193 T + ++ + P G + +I + L ++ Sbjct: 139 GTPGKYSMPVPMMNIINGGEHADNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLK 198 Query: 194 RTVFIPQPNVESAVIRLKVRERPPVEVAD--EKHFFEVVQASFTQRRKTIANNLKARFFP 251 E + V K + T A+ Sbjct: 199 AKGMNTAVGDEGGYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYK--DG 256 Query: 252 EEGRERLESLLAAAGIEPSRRGETLSLEEYARL 284 + + + E + E L+ + Sbjct: 257 KYVLAGEGNKAFTS-EEFTHFLEELTKQYPIVS 288 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,988,375 Number of extensions: 709320 Number of successful extensions: 1569 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1544 Number of HSP's successfully gapped: 16 Length of query: 294 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 188 Effective length of database: 26,153,027 Effective search space: 4916769076 Effective search space used: 4916769076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)