BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0005.p (463 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH1589 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 142 9e-34 MA3022 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryla... 139 1e-32 MK0892 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryla... 135 1e-31 MA3025 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryla... 133 7e-31 PAB1115 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 130 6e-30 >MTH1589 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 268 Score = 142 bits (359), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 0/471 (0%), Positives = 0/471 (0%), Gaps = 87/471 (18%) Query: 2 AVVLAAGQGKRMKSKLY---KVLHPVCGKPMVGHVLDTVKATGCQRTVVVVGHGAEKVKA 58 V L+ + ++ + V + + + Sbjct: 3 RVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILT 62 Query: 59 YVGQ------DAEYVLQETQLGTGHAVKQAKDLLGGEAGTTIVICGDTPLVTAETLEGLM 112 Y+ +++E + + G+ + Sbjct: 63 YMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKP---VTGEYR--QGLTVKRVKKG 117 Query: 113 --ALHEGQSAAATVLTAVMDQPAGYGRIIRGEDGGVQKIVEQKDCTPEEAAVNEINTGTY 170 A ++ + + ++ ++T Sbjct: 118 TLVDIGADKLALCREKLTVN-RIMSF-RVVRLGKEILIEPDEP--EDRYWGYEVLDTRRN 173 Query: 171 CFDNAKLFAALDKVTNSNNQQEYYLTDVIGILRAQGDIVLGYQAHDAAESIGVNDRLALS 230 ++ D + ++ +T ++ A + Sbjct: 174 LAESL-KTVGADV-VVATSRNASPITSIL-DEVKTRMRGAREAAIL-FGGPYKGLPEIDA 229 Query: 231 EA-ESLMRQRIN--RQHMLNGVTIIDPASTYIAADVVIGADTTLYPGTVLKGKTVIGEDC 287 + + + + + + + Sbjct: 230 DIWVNTLPGQCTETVRTEEAVLATLSV-------- 281 Query: 288 IIGPSSEIENCVIMNGAEVKHSVLNQAQVGARTSVGPFAYLRPGAVLGEEVKIGDFVEIK 347 Sbjct: 282 --------------------------------- 308 Query: 348 NASIGDGSKVSHLSYVGDATVGKNVNVGCGAITVN--YD---------------GYNKSR 390 Sbjct: 309 368 Query: 391 TEIGDDAFIGSNVNLVAPVTVGKGAFVVAGSTITRSVSENDLAIARVRQEN 441 Sbjct: 369 -- 417 >MA3022 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 405 Score = 139 bits (349), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 126/455 (27%), Positives = 216/455 (47%), Gaps = 74/455 (16%) Query: 2 AVVLAAGQGKRMKSKLY---KVLHPVCGKPMVGHVLDTVKATGCQRTVVVVGHGAEKVKA 58 A++LAAG+G R + KV+ PV +P++ HV+ +++ G + +++VG+ E++ Sbjct: 3 AIILAAGEGLRCRPLTLTRSKVMLPVANRPILEHVISSLEKNGIKEIILIVGYEKERIMN 62 Query: 59 YV------GQDAEYVLQETQLGTGHAVKQAKDLLGGEAGTTIVICGDTPLVTAETLEGLM 112 Y G + YV Q+ QLGT HA++QAK L+ E +V+ GD LV ++T+ L+ Sbjct: 63 YFEDGLNFGVNITYVEQKAQLGTAHAIEQAKKLISPEDSEFLVLNGDN-LVESKTIADLL 121 Query: 113 ALHEGQSAAATVLTAVMDQPAGYGRIIRGEDGGVQKIVEQKDCTPEEAAVNEINTGTYCF 172 ++G A++LT M+ AGYG +++ E+ V +I+E++ P + + + +NTG Y F Sbjct: 122 NNYKGD---ASLLTVRMEDTAGYGVVLK-ENKRVTRILEKR---PGDLS-HIVNTGIYIF 173 Query: 173 DNAKLFAALDKVTNSNNQQEYYLTDVIGILRAQGDIVLGYQAHDAA-ESIGVNDRL-ALS 230 ++F ++K S N EY +TD + ++ +G IV +++ D L A S Sbjct: 174 -TPQVFETIEKTPISENG-EYAITDTLQLMIDEGKIVTSIPTESKWLDAVHAWDLLKANS 231 Query: 231 EAESLMRQRINRQHMLNGVTIIDPASTYIAADVVIGADTTLYPGTVLKGKTVIGEDCIIG 290 + R + GV I V IG ++ + GT + G VIGE+C IG Sbjct: 232 TVLNSSRNLKQEGELEEGVII--------RGKVAIGKNSRIRAGTYIVGPVVIGENCDIG 283 Query: 291 PSSEIENCVIMNGAEVKHSVLNQAQVGARTSVGPFAYLRPGAVLGEEVKIGDFVEIKNAS 350 P N VI+ P +G+ V I F EI+N+ Sbjct: 284 P-----NVVIL----------------------------PSTTIGDNVSIRSFTEIQNSI 310 Query: 351 IGDGSKVSHLSYVGDATVGKNVNVGCG---------AITVNYDGYNKSR--TEIGDDAFI 399 I + ++S + + +G N +G G I +N ++ + T GDD I Sbjct: 311 IMNDCRISSHGQISNCIIGSNNTLGPGFTAEEKENLEININCKIHSAPKLGTIFGDDNRI 370 Query: 400 GSNVNLVAPVTVGKGAFVVAGSTITRSVSENDLAI 434 G V + A V + V +G+TI R + + + + Sbjct: 371 GGRVLVKAGVMIAVNCQVESGNTIYRDLPRDSVVL 405 >MK0892 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 425 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 125/391 (31%), Positives = 182/391 (46%), Gaps = 58/391 (14%) Query: 1 MAVVLAAGQGKRMK---SKLYKVLHPVCGKPMVGHVLDTVKATGCQRTVVVVGHGAEKVK 57 + +VLAAG+G RM+ KVL PV + ++ ++ +K G + VVVV + AEKV+ Sbjct: 2 IGIVLAAGEGTRMRPLTKTRPKVLLPVADRRLIDFSIEAMKRIGVEHLVVVVEYLAEKVE 61 Query: 58 AYVGQ------DAEYVLQETQLGTGHAVKQAKDLLGGEAGTTIVICGDTPLVTAETLEGL 111 YV + E+V Q LGT HAV A + E T+VI + +E LE Sbjct: 62 RYVKDRWGDSFELEFVRQGKPLGTAHAVYVAWREI--EPDETVVITNGDLVFDSELLERA 119 Query: 112 MALHEGQSAAATVLTAVMDQPAGYGRIIRGEDGGVQKIVEQKDCTPEEAAVNEINTGTYC 171 + HEG A+ VL V D P+ +G + R +DG V ++VE+ PEEA N N G Y Sbjct: 120 VREHEG--VASMVLVEVED-PSEFG-VARLQDGYVVELVEKP--KPEEAPSNLANAGVYV 173 Query: 172 FDNAKLFAALDKVTNSNNQQEYYLTDVIGILRAQGDIVLGYQAHDAAESIGVNDRLALSE 231 + F + + + E+ +TD + + A + VLG +G L + Sbjct: 174 AEPE--FERFLERVKPSPRGEFEITDAL-LDAAIDEGVLGISYDGFWSDVGRPWDLLDAN 230 Query: 232 AESLMRQRINRQHMLNGVTIIDPASTYIAADVVIGADTTLYPGTVLKGKTVIGEDCIIGP 291 A +L R ++R + GV + + V + D L G V++G IG C IGP Sbjct: 231 AWAL-RNAMSRPE-VEGVI---EENVELRGPVWVAEDAILRSGAVVEGPAYIGPGCEIGP 285 Query: 292 SSEIENCVIMNGAEVKHSVLNQAQVGARTSVGPFAYLRPGAVLGEEVKIGDFVEIKNASI 351 NC Y+RP L +V+IG VEIKN+ I Sbjct: 286 -----NC----------------------------YIRPATTLVRDVRIGQAVEIKNSII 312 Query: 352 GDGSKVSHLSYVGDATVGKNVNVGCGAITVN 382 +G+ VSHLSYVGD+ +G N+G G I N Sbjct: 313 MEGTNVSHLSYVGDSVIGAKCNLGAGTIIAN 343 >MA3025 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 397 Score = 133 bits (334), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 126/453 (27%), Positives = 208/453 (45%), Gaps = 91/453 (20%) Query: 2 AVVLAAGQGKRMK---SKLYKVLHPVCGKPMVGHVLDTVKATGCQRTVVVVGHGAEKVKA 58 AVVL AG+G RM+ S KV+ V KP++ H+L++ G + + + G+ E++KA Sbjct: 3 AVVLVAGKGTRMEPLTSDCPKVMLKVANKPILEHILNSAIEAGIEGFIFITGYLEEQIKA 62 Query: 59 YVGQDA------EYVLQETQLGTGHAVKQAKDLLGGEAGTTIVICGDTPLVTAETLEGLM 112 + G + EYV Q+ QLGT +A+ A+ G G +V+ GD L+ E L+ L+ Sbjct: 63 HFGDGSKWEVSIEYVQQKEQLGTANAIGYAR---GHVEGAFLVLNGDM-LIEQEDLKALV 118 Query: 113 ALHEGQSAAATVLTAVMDQPAGYGRIIRGEDGGVQKIVEQKDCTPEEAAVNEINTGTYCF 172 + E A + ++ P+ +G ++ E+ V +I+E+ P+ N N G Y F Sbjct: 119 SREE----EAVICVKEVENPSDFG-VLETENDKVVRIIEK----PKNPPTNLANAGIYLF 169 Query: 173 DNAKLFAALDKVTNSNNQQEYYLTDVIGILRAQGDIVLGYQAHDAAESIGVNDRLALSEA 232 + +F +DK T + ++E +TD I +L G V GY + + I + L +A Sbjct: 170 RES-IFDFIDK-TQPSVRRELEITDSIQMLINSGAPV-GYSSLE-GRWIDIGYPWDLLKA 225 Query: 233 ESLMRQRINRQHMLNGVT-----IIDPASTYIAADVVIGADTTLYPGTVLKGKTVIGEDC 287 +H+L + ++P + I +V IG + + G+ ++G VIGE+C Sbjct: 226 ---------NEHLLKDLKGSCEGTVEPYAI-IKGEVSIGKGSLIRSGSYIEGPVVIGENC 275 Query: 288 IIGPSSEIENCVIMNGAEVKHSVLNQAQVGARTSVGPFAYLRPGAVLGEEVKIGDFVEIK 347 IGP NC ++RP + ++IG+ VE+K Sbjct: 276 DIGP-----NC----------------------------FIRPSTAIDNHIRIGNAVEVK 302 Query: 348 NASIGDGSKVSHLSYVGDATVGKNVNVGCGAITVN--YDGYN---------------KSR 390 N I + + V HLSYVGD+ +G + N G G N +DG N K Sbjct: 303 NTIILEDTHVGHLSYVGDSVIGHHCNFGAGTKVANLRHDGKNIKVMLRGRILDSGRRKLG 362 Query: 391 TEIGDDAFIGSNVNLVAPVTVGKGAFVVAGSTI 423 +GDD G N ++ V + KG + G + Sbjct: 363 VIMGDDVHTGINTSINIGVVMHKGRATLPGEVV 395 >PAB1115 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 419 Score = 130 bits (326), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 130/465 (27%), Positives = 209/465 (44%), Gaps = 86/465 (18%) Query: 2 AVVLAAGQGKRMK---SKLYKVLHPVCGKPMVGHVLDTVKATGCQRTVVVVGHGAEKV-- 56 AVVLAAG+G+R++ K + V KP++ ++L+ + +++V + EK+ Sbjct: 3 AVVLAAGKGERLRPLTDDRPKPILKVANKPIIEYILENLDPF-VDEFIIIVKYMKEKIIE 61 Query: 57 ---KAYVGQDAEYVLQETQLGTGHAVKQAKDLLGGEAGTTIVICGDTPLVTAETLEGLMA 113 + G+ YV Q + GT A+ AKD + E I GD E + GL+ Sbjct: 62 TLGDEFHGKPITYVSQVEEEGTAKAIYSAKDYI--EEEEFFAINGDI-YFEQEAIRGLLH 118 Query: 114 LHEGQSAAATVLTAVMDQPAGYGRIIRGEDGGVQKIVEQKDCTPEEAAVNEINTGTYCFD 173 +++ A A ++ D + G +++ E G V++IVE+ A N G Y F Sbjct: 119 VYKKTKANAALVVKKFDDLSQLG-MVKTEGGYVREIVEKPGNVGGLA-----NLGIYIF- 171 Query: 174 NAKLFAALDKVTNSNNQQEYYLTDVIGILRAQGDIVLGYQAHDAAESIGVNDRLALSEAE 233 N +F +++ T + + EY +TD I ++ + G V ++ IG L L E Sbjct: 172 NPDVFEFIER-TQESERGEYEITDTINLMISDGRKVAYFEYSGFWSDIGRPWDL-LEVNE 229 Query: 234 SLMRQRIN---RQHMLNGVTIIDPASTYIAADVVIGADTTLYPGTVLKGKTVIGEDCIIG 290 +++ ++ R + G TII P V IG T + G+ + G IG++C IG Sbjct: 230 YILKTKLKHDIRGTVEEGATIIPP--------VEIGEGTVVRSGSYIIGPVKIGKNCRIG 281 Query: 291 PSSEIENCVIMNGAEVKHSVLNQAQVGARTSVGPFAYLRPGAVLGEEVKIGDFVEIKNAS 350 P NC ++RP +G+ IG+ VEIKN+ Sbjct: 282 P-----NC----------------------------FIRPYTSIGDNCHIGNAVEIKNSI 308 Query: 351 IGDGSKVSHLSYVGDATVGKNVNVGCGAITVNYDGYNKS-------------RTEIGDDA 397 I D S HL+YVGD+ VG+N N+G G IT N N++ R ++G A Sbjct: 309 IMDNSNAPHLNYVGDSIVGENTNLGAGTITANLRHDNRTIKVEIKGKLEDSGRRKLG--A 366 Query: 398 FIGSNVNLVAPVT------VGKGAFVVAGSTITRSVSENDLAIAR 436 IG NV VT +G GA V G + +++ N L +A+ Sbjct: 367 IIGHNVKTGINVTIYPGRKIGSGALVGPGVIVDKNIPPNVLVVAK 411 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,348,004 Number of extensions: 1452060 Number of successful extensions: 2857 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 2847 Number of HSP's successfully gapped: 51 Length of query: 463 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 353 Effective length of database: 25,691,691 Effective search space: 9069166923 Effective search space used: 9069166923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)