BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0027.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2180 [R] COG1073 Hydrolases of the alpha/beta superfamily 279 5e-75 L15267 [R] COG1073 Hydrolases of the alpha/beta superfamily 239 4e-63 L123536 [R] COG1073 Hydrolases of the alpha/beta superfamily 207 2e-53 BS_yqkD [R] COG1073 Hydrolases of the alpha/beta superfamily 124 2e-28 CAC2725 [R] COG1073 Hydrolases of the alpha/beta superfamily 109 6e-24 >lin2180 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 319 Score = 279 bits (713), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 137/295 (46%), Positives = 191/295 (64%), Gaps = 4/295 (1%) Query: 20 AGFFFYGIAIKRAPKEFLAK-TPDLKVDP-PVAGPSWGEGADWVSRHSFRKIEITSDDGL 77 A F+ Y A+ R K T D +A + E W+ + K TS DGL Sbjct: 23 ASFYLYSYALSRDNSSMNDKPTADQTTKTAKLAKKNRQENIAWMEQQRLTKWTETSADGL 82 Query: 78 TLRGYYLPSERAAGRTAIIAHGYSGK-GK-DMGAYAKIYYESLGYHVLLPDARGHGDSTG 135 L YL + + T I+AHGY GK GK +M A++Y + GY+VL+PDAR HG+S G Sbjct: 83 KLVANYLEAPSPSNTTIILAHGYRGKSGKVEMAGLARMYNQKFGYNVLMPDARAHGESEG 142 Query: 136 EYIGFGWPERRDYLRWIEYILAESGPEAQIVLHGVSMGGATVMMTSGEELPPQVKAIVAD 195 + IGFGWPER+DY++W +L ++G + +IVLHGVSMG +TV+MTS E+LP QVK+I+AD Sbjct: 143 KNIGFGWPERKDYVQWTNQVLDKNGDDTEIVLHGVSMGSSTVLMTSAEKLPKQVKSIIAD 202 Query: 196 CGYTSVKAQLSYQLWRMYHLPSFPFVHMASFITRIKAGYFFGEASALKQVRKARVPILFI 255 CGYTS+ A+L+YQL M+HLP FP + MAS I ++K G+FF EASA+ V + +PI +I Sbjct: 203 CGYTSMDAELAYQLKAMFHLPKFPIIPMASLINKVKEGFFFSEASAINAVAETDLPIFYI 262 Query: 256 HGDADKFVPFYMMDKLYQACRSPKDQLVIHGAGHGVAYDTDKAAYVGKVTDFVTR 310 HGD+D FVP M+++LY A +S K++ V+ GA HG A+ + Y KV F+T+ Sbjct: 263 HGDSDAFVPTSMVEELYDATKSYKEKWVVKGAEHGQAFTVEPETYEEKVRQFLTK 317 >L15267 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 320 Score = 239 bits (610), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 118/244 (48%), Positives = 166/244 (68%), Gaps = 4/244 (1%) Query: 71 ITSDDGLTLRGYYLPSER--AAGRTAIIAHGYSGKGKDMGAYAKIYYESLGYHVLLPDAR 128 +TS D L L+ Y ++ + AI+ HGY G+ DM ++ + +Y GY+V+ PD R Sbjct: 78 VTSSDKLKLKLYAADYKQPTKTNKWAIVVHGYGGQSIDMASWTRHFYNK-GYNVVTPDLR 136 Query: 129 GHGDSTGEYIGFGWPERRDYLRWIEYILAESGPEAQIVLHGVSMGGATVMMTSGEELPPQ 188 GHG S G+YIG GW +R+D L WI I+ + GP+A+IVL GVSMGGATVM TSGE+LP Sbjct: 137 GHGKSQGDYIGMGWDDRKDMLLWIAKII-QKGPQAEIVLLGVSMGGATVMNTSGEKLPSN 195 Query: 189 VKAIVADCGYTSVKAQLSYQLWRMYHLPSFPFVHMASFITRIKAGYFFGEASALKQVRKA 248 VKAIV DCG+TS +YQL ++Y LP FP ++ A+ + +++AGY ++SA+KQV K+ Sbjct: 196 VKAIVEDCGFTSTVDVFAYQLKQLYGLPKFPVLYAANTVVKMRAGYDIFKSSAIKQVAKS 255 Query: 249 RVPILFIHGDADKFVPFYMMDKLYQACRSPKDQLVIHGAGHGVAYDTDKAAYVGKVTDFV 308 + PILFIHGD D FVPF M++ LY A + K++L++HGAGHG + + Y V DFV Sbjct: 256 KTPILFIHGDKDTFVPFKMLNPLYDAAKVEKEKLIVHGAGHGESEKVNPDLYWSHVWDFV 315 Query: 309 TRYV 312 +Y+ Sbjct: 316 GKYM 319 >L123536 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 311 Score = 207 bits (527), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 111/295 (37%), Positives = 173/295 (58%), Gaps = 15/295 (5%) Query: 20 AGFFFYGIAIKRAPKEFLAKTPDLKVDPPVAGPSWGEGADWVSRHSFRKIEITSDDGLTL 79 A +FY IA R ++ +K P S + D +S+ + E +DGL L Sbjct: 27 ATLYFYQIACVRNNQQI----GQVKSSSP--NYSLVQKFDKLSKST----ESIKNDGLKL 76 Query: 80 RGYYLPSERAAGRTAIIAHGYSGKGKDMGAYAKIYYESLGYHVLLPDARGHGDSTGEYIG 139 +Y+P+E T I+ HG+ M Y ++++E LGY+VL+PD RG G S G++I Sbjct: 77 DAWYVPAEHKTNNTVIVVHGFRQDKSAMRQYGQLFHE-LGYNVLMPDNRGAGQSEGKFIT 135 Query: 140 FGWPERRDYLRWIEYILAESGPEAQIVLHGVSMGGATVMMTSGEE-LPPQVKAIVADCGY 198 FG+ ++ D + W Y L + PE+QI L+G+SMG +TVMM S E+ LP VK I+ DCGY Sbjct: 136 FGYHDKFDVIAWANY-LTDKNPESQISLYGLSMGASTVMMASSEKSLPSSVKNIIEDCGY 194 Query: 199 TSVKAQLSYQLWRMYHLPSFPFVHMASFITRIKAGYFFGEASALKQVRKARVPILFIHGD 258 T+ +++YQ Y++P FP V+ S ++++ G+FF EASA K + K ++PIL IHG Sbjct: 195 TNAWDEITYQAKESYNIPPFPLVYSVSLESKLRQGWFFQEASATKALTKDKLPILLIHGS 254 Query: 259 ADKFVPFYMMDKLYQACR--SPKDQLVIHGAGHGVAYDTDKAAYVGKVTDFVTRY 311 D +VP M+ + Y+A + +PK+ LVI GA H +++T+ Y ++ F+ Y Sbjct: 255 NDTYVPTSMVYENYKAVKKGTPKELLVIKGAAHAKSFETNPELYRQTISKFMKAY 309 >BS_yqkD [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 305 Score = 124 bits (310), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 76/270 (28%), Positives = 128/270 (47%), Gaps = 9/270 (3%) Query: 44 KVDPPVAGPSWGEGADWVSRHSFRKIE-----ITSDDGLTLRGYYLPSERAAGRTAIIAH 98 K D + G D SF ++E I S G ++GY++ T II H Sbjct: 32 KTDEDIIKRETDNGHDVFE--SFEQMEKTAFVIPSAYGYDIKGYHVAPHDTPN-TIIICH 88 Query: 99 GYSGKGKDMGAYAKIYYESLGYHVLLPDARGHGDSTGEYIGFGWPERRDYLRWIEYILAE 158 G + + Y ++ + LG++VL+ D R HG S G+ +G+ E+ D + + + + Sbjct: 89 GVTMNVLNSLKYMHLFLD-LGWNVLIYDHRRHGQSGGKTTSYGFYEKDDLNKVVSLLKNK 147 Query: 159 SGPEAQIVLHGVSMGGATVMMTSGEELPPQVKAIVADCGYTSVKAQLSYQLWRMYHLPSF 218 + I +HG SMG T ++ +G +ADC + QL+Y+L Y LPS+ Sbjct: 148 TNHRGLIGIHGESMGAVTALLYAGAHCSDGADFYIADCPFACFDEQLAYRLRAEYRLPSW 207 Query: 219 PFVHMASFITRIKAGYFFGEASALKQVRKARVPILFIHGDADKFVPFYMMDKLYQACRSP 278 P + +A F +++ GY E S L + K P+LFIH D ++P ++LY+ R P Sbjct: 208 PLLPIADFFLKLRGGYRAREVSPLAVIDKIEKPVLFIHSKDDDYIPVSSTERLYEKKRGP 267 Query: 279 KDQLVIHGAGHGVAYDTDKAAYVGKVTDFV 308 K + H ++Y ++ Y V +F+ Sbjct: 268 KALYIAENGEHAMSYTKNRHTYRKTVQEFL 297 >CAC2725 [R] COG1073 Hydrolases of the alpha/beta superfamily Length = 330 Score = 109 bits (272), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 77/245 (31%), Positives = 121/245 (49%), Gaps = 7/245 (2%) Query: 69 IEITSDDGLTLRGYYLPSERAAGRTAIIAHGYSGKGKDMGAYAKIYYESLGYHVLLPDAR 128 + I S+ G L G Y+ + + +T II HG +G + YA IY + LGY+VL+ D+R Sbjct: 69 VVIESNFGYKLSGTYIKNFVDSRKTIIIVHGITGSRWESMKYADIYLD-LGYNVLIYDSR 127 Query: 129 GHGDSTGEYIGFGWPERRDYLRWIEYILAESGPEAQIVLHGVSMGGATVMMTSG-EELPP 187 HG S G I G+ E+ D ++++ ++ P I +HG SMG AT ++ S E Sbjct: 128 YHGVSGGNDITLGYFEKYDLNNCVKWVKNKT-PGGIIGIHGESMGAATALLQSNMNEKTK 186 Query: 188 QVKAIVADCGYTSVKA----QLSYQLWRMYHLPSFPFVHMASFITRIKAGYFFGEASALK 243 V V DC ++ + +L+Y++ + + V +S I KAG+ S +K Sbjct: 187 DVSFYVVDCPFSDLPQLFGEKLNYEMKNHGAVVAKMVVFYSSLIAFFKAGFSVYAISPIK 246 Query: 244 QVRKARVPILFIHGDADKFVPFYMMDKLYQACRSPKDQLVIHGAGHGVAYDTDKAAYVGK 303 ++ + PI+F HG D +P M LY + K + AGH AY +K Y K Sbjct: 247 AIQDVKTPIMFAHGADDDLIPPEMSVDLYSIKKGAKYLYIAPNAGHAEAYLKNKTDYTNK 306 Query: 304 VTDFV 308 V F+ Sbjct: 307 VRQFL 311 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,007,345 Number of extensions: 1115921 Number of successful extensions: 2151 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2140 Number of HSP's successfully gapped: 13 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)