BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0020.p (263 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2748 [P] COG0619 ABC-type cobalt transport system, permease c... 179 3e-45 BS_ybaF [P] COG0619 ABC-type cobalt transport system, permease c... 176 4e-44 lin2783 [P] COG0619 ABC-type cobalt transport system, permease c... 174 2e-43 CAC3100 [P] COG0619 ABC-type cobalt transport system, permease c... 152 5e-37 L77627 [P] COG0619 ABC-type cobalt transport system, permease co... 149 3e-36 >lin2748 [P] COG0619 ABC-type cobalt transport system, permease component CbiQ and related transporters Length = 265 Score = 179 bits (455), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 87/248 (35%), Positives = 149/248 (60%) Query: 1 MNERLLLGRSIETGSWVHKLDARSKLTGMLLYVAVILLSDSWQSMALLAVFSIAVMASTR 60 M E+++LGR I SW+H++D R+K+T ++ ++A++ L+++W + AL+ + + ++ +++ Sbjct: 1 MMEKMILGRYIPGNSWLHRIDPRAKITAVMAFIAIVFLANNWLTYALMFAYVLYLVLTSK 60 Query: 61 IPLKYFIKAAKPLRYLMLFIFIVQLLSVKAGDVLWSAGSFSLHEGGLRLASFSVIRMXXX 120 +P +FIK +P+ +L+L ++Q+ K G VL G + GL + R Sbjct: 61 VPFLFFIKGLQPIFWLILITLLLQVFFTKGGTVLVDLGLLQITTLGLANGAMMFCRFVLI 120 Query: 121 XXXXXXXXXXXXPGKLNQGLEGILSPFKKFGLSPDRITLMISIALRFIPTILDESQIIMK 180 P +L GLE IL+PF+ L + LM+SI+LRFIPT++DE++ I+K Sbjct: 121 IFMTTLLTLTTSPIELTDGLEKILAPFRLVHLPVHELALMLSISLRFIPTLMDETEKILK 180 Query: 181 AQASRGADLQELPLKEKARMLVSLLVPVISSAFRRAQDLVYSMEARGFRMDAPRSRYHRL 240 AQ +RG + ++ + ++ LLVP+ SAF+RA+DL +MEARG+R R+R+ L Sbjct: 181 AQKARGVEFTSGKWSDRIKAIIPLLVPLFISAFKRAEDLAIAMEARGYRGGKGRTRFRLL 240 Query: 241 RWGAADTV 248 RW ADT Sbjct: 241 RWRFADTC 248 >BS_ybaF [P] COG0619 ABC-type cobalt transport system, permease component CbiQ and related transporters Length = 265 Score = 176 bits (445), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 90/256 (35%), Positives = 157/256 (61%) Query: 1 MNERLLLGRSIETGSWVHKLDARSKLTGMLLYVAVILLSDSWQSMALLAVFSIAVMASTR 60 M + +++G+ + S VH+LD R+KL + L+V ++ L+++ Q+ ALL +F+I V++ TR Sbjct: 1 MMDSMIIGKYVPGTSLVHRLDPRTKLITIFLFVCIVFLANNVQTYALLGLFTIGVVSLTR 60 Query: 61 IPLKYFIKAAKPLRYLMLFIFIVQLLSVKAGDVLWSAGSFSLHEGGLRLASFSVIRMXXX 120 +P + +K KP+ +++LF F++ +L G +++ G ++EGGL F +R Sbjct: 61 VPFSFLMKGLKPIIWIVLFTFLLHILMTHEGPIIFQIGFSRVYEGGLVQGIFISLRFVYL 120 Query: 121 XXXXXXXXXXXXPGKLNQGLEGILSPFKKFGLSPDRITLMISIALRFIPTILDESQIIMK 180 P ++ G+E +L+P KK L + LM+SI+LRFIPT+++E+ IMK Sbjct: 121 ILITTLLTLTTTPIEITDGMEQLLNPLKKLKLPVHELALMMSISLRFIPTLMEETDKIMK 180 Query: 181 AQASRGADLQELPLKEKARMLVSLLVPVISSAFRRAQDLVYSMEARGFRMDAPRSRYHRL 240 AQ +RG D P+KE+ + +V LLVP+ SAF+RA++L +MEARG++ R++Y +L Sbjct: 181 AQMARGVDFTSGPVKERVKAIVPLLVPLFVSAFKRAEELAVAMEARGYQGGEGRTKYRKL 240 Query: 241 RWGAADTVFVAMFVIM 256 W DT + +++ Sbjct: 241 VWTGKDTSVIVSLIVL 256 >lin2783 [P] COG0619 ABC-type cobalt transport system, permease component CbiQ and related transporters Length = 266 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 90/248 (36%), Positives = 141/248 (56%) Query: 1 MNERLLLGRSIETGSWVHKLDARSKLTGMLLYVAVILLSDSWQSMALLAVFSIAVMASTR 60 M E+L+LGR + S +H LDAR+KL Y+ ++ L+++W + AL+ +F++ ++ T Sbjct: 1 MIEKLILGRFVPGESLIHGLDARTKLLAGFYYIGILFLANNWWTYALMVLFTLMIVQMTG 60 Query: 61 IPLKYFIKAAKPLRYLMLFIFIVQLLSVKAGDVLWSAGSFSLHEGGLRLASFSVIRMXXX 120 I LK FIK KPL +L+LF ++Q+L G + + G F++ GL F +R Sbjct: 61 IKLKVFIKGVKPLIWLILFTVVMQILFASGGTIYFDWGPFTISSFGLLNGVFVFLRFVLI 120 Query: 121 XXXXXXXXXXXXPGKLNQGLEGILSPFKKFGLSPDRITLMISIALRFIPTILDESQIIMK 180 P L + IL PF + + I LMIS+ALRFIPT++ E+ IMK Sbjct: 121 IIMSTVITLTTTPMNLTDAIAYILRPFAVLKVPVNDIALMISVALRFIPTLMGETDKIMK 180 Query: 181 AQASRGADLQELPLKEKARMLVSLLVPVISSAFRRAQDLVYSMEARGFRMDAPRSRYHRL 240 AQ +RG D E L E+ +++V + +P+ S+F RA++L +MEARG++ R+R+ L Sbjct: 181 AQRARGVDFGEGNLFEQMKVVVPIFIPLFVSSFNRAEELADAMEARGYQGGEGRTRFRIL 240 Query: 241 RWGAADTV 248 W D V Sbjct: 241 HWHFGDLV 248 >CAC3100 [P] COG0619 ABC-type cobalt transport system, permease component CbiQ and related transporters Length = 267 Score = 152 bits (384), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 146/251 (58%), Gaps = 1/251 (0%) Query: 1 MNERLLLGRSIETGSWVHKLDARSKLTGMLLYVAVILLSDSWQSMALLAVFSIAVMASTR 60 M + + +G+ I S++HKLD R+K+ L+Y+ + L D ++ + F++ ++ + Sbjct: 1 MIKDITIGQYIPGKSFIHKLDPRTKILLSLVYIVNLFLIDKFKGYIFITAFTLFIIFISG 60 Query: 61 IPLKYFIKAAKPLRYLMLFIFIVQL-LSVKAGDVLWSAGSFSLHEGGLRLASFSVIRMXX 119 I +KY K+ KP+ L++F + + ++ A L+ +++ GL LA+F IR+ Sbjct: 61 IKMKYIYKSLKPILLLIIFTGALNIFMTPGAAAPLFHWSFIHIYKEGLVLAAFMSIRLIF 120 Query: 120 XXXXXXXXXXXXXPGKLNQGLEGILSPFKKFGLSPDRITLMISIALRFIPTILDESQIIM 179 P +L G+E +L+PF K GL +M++IALRFIPT++DE+ IM Sbjct: 121 LIIGTSMLTYTTSPIELTDGIEKLLTPFSKIGLPAHEFAMMMTIALRFIPTLIDETDKIM 180 Query: 180 KAQASRGADLQELPLKEKARMLVSLLVPVISSAFRRAQDLVYSMEARGFRMDAPRSRYHR 239 KAQ +RGAD+ + +KA+ L+ +LVP+ S+FRRA +L +ME+R +R R+R + Sbjct: 181 KAQMARGADIDSGNIIKKAKSLIPILVPLFISSFRRADELAMAMESRCYRGGEGRTRMKQ 240 Query: 240 LRWGAADTVFV 250 L++ + D V V Sbjct: 241 LKFTSRDCVAV 251 >L77627 [P] COG0619 ABC-type cobalt transport system, permease component CbiQ and related transporters Length = 266 Score = 149 bits (377), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 83/249 (33%), Positives = 139/249 (55%) Query: 3 ERLLLGRSIETGSWVHKLDARSKLTGMLLYVAVILLSDSWQSMALLAVFSIAVMASTRIP 62 + +L+GR I S +H++D RSKL M+ +V +I L+ W LL ++++A + ++I Sbjct: 2 QNMLMGRYIPGDSIIHRMDPRSKLLVMIAFVVIIFLAHDWLGYLLLVLYTLAGVLLSKIS 61 Query: 63 LKYFIKAAKPLRYLMLFIFIVQLLSVKAGDVLWSAGSFSLHEGGLRLASFSVIRMXXXXX 122 + YF+K +P+ L+LF I Q+L V++S + L A + R Sbjct: 62 VSYFLKGLRPMIGLILFTVIFQMLFTNGQHVIFSLWFIKISTESLINAVYIFFRFVLIIF 121 Query: 123 XXXXXXXXXXPGKLNQGLEGILSPFKKFGLSPDRITLMISIALRFIPTILDESQIIMKAQ 182 P L G+E L P KK + + LM+SI+LRFIPT++D++ +IM AQ Sbjct: 122 MSTILTLTTPPLTLADGIEKGLGPLKKIKVPVHELGLMLSISLRFIPTLMDDTTMIMNAQ 181 Query: 183 ASRGADLQELPLKEKARMLVSLLVPVISSAFRRAQDLVYSMEARGFRMDAPRSRYHRLRW 242 +RG D E L +K + ++ +L+P+ S+FRRA DL +ME+RG++ R++Y +L+W Sbjct: 182 KARGMDFGEGNLLKKIKSVIPILIPLFVSSFRRADDLAVAMESRGYQGGDGRTKYRQLKW 241 Query: 243 GAADTVFVA 251 D++ V Sbjct: 242 KMRDSLLVV 250 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.139 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,679,830 Number of extensions: 539440 Number of successful extensions: 1242 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 1231 Number of HSP's successfully gapped: 22 Length of query: 263 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 159 Effective length of database: 26,383,695 Effective search space: 4195007505 Effective search space used: 4195007505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)