BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0017.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF2176 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 170 2e-42 DR0851 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 154 2e-37 TVN0407 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and ... 110 2e-24 APE1570 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and ... 107 3e-23 Ta1198 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and r... 107 3e-23 >AF2176 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 291 Score = 170 bits (431), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 101/286 (35%), Positives = 151/286 (52%), Gaps = 7/286 (2%) Query: 12 KLKMFSELVMFSHTLFSLPFAVISMVWAAGGWPSGHMMLWGLIALIGARNGANAFNRLVD 71 ++KM+ + + HTLF+LPFA A GG S + L A G R A FNR++D Sbjct: 3 RIKMYLDFIKIEHTLFALPFAYAGAFLAEGGLISLRLAFLILTAFTGLRTAAMTFNRIID 62 Query: 72 RTFDGKNPRTAHRHLPQHLLAEKEVIFFIIINYALFIVASGMXXXXXXXXSPVAIVLISS 131 R D KNPRTA RHLP L++ KE + A++ +++ + SP+ + Sbjct: 63 REIDAKNPRTAMRHLPAGLISLKEAYTIAFLALAIYFISAALINKTALMLSPIPAITAYI 122 Query: 132 YSYTKRFTFLSHLYLGFVIASAPIGAWFAVTGNI-----AFTPFVIGTVVMLWIAGFDII 186 Y Y KRFT L H LG +A AP+G W AVT +I P +IG VM W+AGFD+I Sbjct: 123 YPYLKRFTCLCHYVLGLNLAFAPLGGWIAVTDSIDIFGSELVPTLIGVAVMFWVAGFDMI 182 Query: 187 YGTQDIDFDRRNGLWSIPSFFGLENALRISKGLHFIMIXXXXXXXXXXXXGWMYLVGIGI 246 YG QD+DFDR N L SI + FG+ AL +S+ L+ ++ + + + + Sbjct: 183 YGLQDVDFDRSNNLHSIGAHFGVRAALWLSR-LNHVIFFALIAIALQIHNAKLAELFLPL 241 Query: 247 ATLLLMTEHRIIKP-HNRRLMKVASYNLNQVISMVILLCTLIDYFF 291 LLL EH I++ ++ +++A + +N ++S +L D F Sbjct: 242 IALLLFYEHYIVRDGYDEAKIQIAFFYVNALVSSALLAAIFADAIF 287 >DR0851 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 313 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 98/293 (33%), Positives = 157/293 (53%), Gaps = 7/293 (2%) Query: 2 ILNAVKTSALKLKMFSELVMFSHTLFSLPFAVISMVWAA-----GGWPSGHMMLWGLIAL 56 +++A S ++K + ELV F HT+F+LPFA M+ A+ GWP H ++W +A+ Sbjct: 15 VVSAAARSGTQVKTYLELVKFEHTVFALPFAYAGMLLASMAQNGTGWPGWHTLIWVTVAM 74 Query: 57 IGARNGANAFNRLVDRTFDGKNPRTAHRHLPQHLLAEKEVIFFIIINYALFIVASGMXXX 116 AR A A NR++DR D +NPRTA R +P ++ + + ++ A+ + A+ Sbjct: 75 AAARTAAMAANRVIDRAIDARNPRTAGREVPSGKVSPAQAWGLVAVSVAVLLFAAWQLNP 134 Query: 117 XXXXXSPVAIVLISSYSYTKRFTFLSHLYLGFVIASAPIGAWFAVTGNIAFTPFVIGTVV 176 P+A++ + Y YTKRFT+L H +LG +A G W AVTG+ + + + V Sbjct: 135 LCVALLPLALLFLIGYPYTKRFTWLCHAWLGVTDGAAAAGGWIAVTGHFSPVAWALWATV 194 Query: 177 MLWIAGFDIIYGTQDIDFDRRNGLWSIPSFFGLENALRISKGLHFIMIXXXXXXXXXXXX 236 + W+ G D IY T D FDR NG+ SIP+ FG+ AL I+ H + Sbjct: 195 VFWMIGLDTIYATMDYAFDRANGVKSIPARFGIPAALNIAAVSHVLTFGLLLLVGVLAGA 254 Query: 237 G-WMYLVGIGIATLLLMTEHRIIKPHNRRLMKVASYNLNQVISMVILLCTLID 288 W YL + + +LL EHRI+ P + + VA ++ N +++ +LL ++D Sbjct: 255 SVWYYLAALVMGAILLF-EHRIVDPQDLGRVNVAFFDANMWLAITMLLGVVMD 306 >TVN0407 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 286 Score = 110 bits (276), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 80/282 (28%), Positives = 135/282 (47%), Gaps = 7/282 (2%) Query: 11 LKLKMFSELVMFSHTLFSLPFAVISMVWAAGGWPSGHMMLWGLIALIGARNGANAFNRLV 70 +K K + + HT+F LPF + V AAG + ++ LIA + AR+ A + NR+ Sbjct: 1 MKFKDIVDYIKLEHTVFDLPFILSGYVIAAGTSIFPYKIILILIAAVTARSSAMSINRIE 60 Query: 71 DRTFDGKNPRTAHRHLPQHLLAEKEVIFFIIINYALFIVASGMXXXXXXXXSPVAIVLIS 130 +D NPR L + +E I I+ A+F +++ + SP+ I L Sbjct: 61 GLRYDLTNPRKKEWALVSGRMTVREAIALTILFIAVFEISAFLLNRLVFILSPIVIFLFV 120 Query: 131 SYSYTKRFTFLSHLYLGFVIASAPIGAWFAV----TGNIAFTPFVIGTVVMLWIAGFDII 186 S KR+T H Y+G I + + AV N+A I + LWIAGFD+I Sbjct: 121 SDPILKRYTEWRHFYMGSAIGIGTLAGYLAVIPEFPSNLAIYFLFIAS--SLWIAGFDMI 178 Query: 187 YGTQDIDFDRRNGLWSIPSFFGLENALRISKGLHFIMIXXXXXXXXXXXXGWMYLVGIGI 246 Y DI++D++N L ++ + +G++ L IS H + W YL G+ I Sbjct: 179 YVIPDIEYDQKNKLKTVMTKYGIKLGLLISDITHLAALIFFWLMLYYFRTIW-YLAGLII 237 Query: 247 ATLLLMTEHRIIKPHNRRLMKVASYNLNQVISMVILLCTLID 288 T+L++ +H I+ P + ++ + +N N I + L+ ++ Sbjct: 238 ITILVIYQHIIVDPADPLSIRKSFFNANSFIGFIFLITVILS 279 >APE1570 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 284 Score = 107 bits (266), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 65/199 (32%), Positives = 99/199 (49%), Gaps = 9/199 (4%) Query: 18 ELVMFSHTLFSLPFAVISMVWAAGGWPSGHMMLWGLIALIGARNGANAFNRLVDRTFDGK 77 LV HT+FSLPFA + + + + +L A++G R A+N + D D Sbjct: 2 RLVRIEHTIFSLPFAYVGALLSRYPFTLADAILMA-AAVVGLRMAGMAYNNIADLDIDRL 60 Query: 78 NPRTAHRHLPQHLLAEKEVIFFIIINYALFIVASGMXXXXXXXXSPVAIVLISSYSYTKR 137 NPRTA R L ++ +E + A++ ++ + SP+ + + +Y + KR Sbjct: 61 NPRTAKRPLVVGAVSLREAWALVAAGSAIYFASAALLNTYALLLSPLVLAIALTYPHAKR 120 Query: 138 FTFLSHLYLGFVIASAPIGAWFAVTGNIAFT--------PFVIGTVVMLWIAGFDIIYGT 189 L HL+LG V+ S G A +G+ A + P++ V LW+AGFD IY Sbjct: 121 LHPLPHLHLGIVLGSVVFGGAVAASGDEASSLGEVLRSVPWLYVAAVSLWVAGFDTIYSI 180 Query: 190 QDIDFDRRNGLWSIPSFFG 208 DIDFDR +GL SIP+ G Sbjct: 181 MDIDFDRSHGLGSIPALLG 199 >Ta1198 [H] COG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases Length = 286 Score = 107 bits (266), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 80/276 (28%), Positives = 127/276 (46%), Gaps = 3/276 (1%) Query: 18 ELVMFSHTLFSLPFAVISMVWAAGGWPSGHMMLWGLIALIGARNGANAFNRLVDRTFDGK 77 + + HT+F LPF V AAG + +L LIA + AR A + NR+ +D Sbjct: 8 DYIKLEHTVFDLPFIFTGYVLAAGRYIYPIKILLILIAAVSARASAMSINRIEGLRYDVI 67 Query: 78 NPRTAHRHLPQHLLAEKEVIFFIIINYALFIVASGMXXXXXXXXSPVAIVLISSYSYTKR 137 NPR L ++++E I I ALF +A+ SPV I L + KR Sbjct: 68 NPRKKDWALVSGRISKREAIAMTIFFIALFEMATYFLNRLVFILSPVVIFLFLTDPLLKR 127 Query: 138 FTFLSHLYLGFVIASAPIGAWFAVTGNI--AFTPFVIGTVVMLWIAGFDIIYGTQDIDFD 195 T H+Y+G I + + AV T ++I WIAGFD+IY DI++D Sbjct: 128 ITPWRHVYMGSTIGVGVLAGYLAVIPAFPRELTIYLIFIGSSFWIAGFDVIYVIPDIEYD 187 Query: 196 RRNGLWSIPSFFGLENALRISKGLHFIMIXXXXXXXXXXXXGWMYLVGIGIATLLLMTEH 255 R NGL ++ +GL+ L+IS H + + W YL + + + L++ +H Sbjct: 188 RINGLKTLMVRYGLKRGLQISIIFHAVTLISFWAVMLYVRTYW-YLAAMIVISFLVVYQH 246 Query: 256 RIIKPHNRRLMKVASYNLNQVISMVILLCTLIDYFF 291 I+ P ++ + +N N I + L+ ++ F Sbjct: 247 VILDPSKPETVRRSFFNANSFIGFLYLISLILGIVF 282 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.143 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,788,460 Number of extensions: 763903 Number of successful extensions: 1689 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1677 Number of HSP's successfully gapped: 12 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 115 (48.9 bits)