BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0007.p (220 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2520 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epim... 140 2e-33 PA4017 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epime... 125 6e-29 STM3268 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epim... 99 6e-21 ZyraR [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimer... 97 2e-20 ECs4033 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epim... 97 2e-20 >RSc2520 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 250 Score = 140 bits (352), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 89/216 (41%), Positives = 124/216 (57%), Gaps = 10/216 (4%) Query: 6 VVLGATGLVGSALTEDLLNGEWA-EVRVLVRRPMKLRH----PG-LKQVIIDWERL-AEY 58 +V+GA+GLVG A+ L+ WA V LVRRP L PG L++ ++D+ RL A Sbjct: 28 LVVGASGLVGRAVVRRLVAQTWAGRVTALVRRPGALTAGQALPGRLRECVVDFARLDAPE 87 Query: 59 KEQFAGASAVFCCLGTTIKKAGSQEKFERVDLEYPLAAAALAKTCGVKQFLAVSSMGANV 118 + A V C LGTT++ AGSQ F RVD++YP A A G FL VS++GA+ Sbjct: 88 TQSVLAADIVICALGTTLRDAGSQAAFRRVDVDYPAEIARRAFAAGAHHFLLVSALGADA 147 Query: 119 RSRAFYSRTKGKLENGLGAIGFQGLHLFRPSLLLGDREESRPGERVAAIVMKALDFAMVG 178 RS FY+R KG+ E + ++GF + + RPSLLLG+R R ERVA + KA+ +V Sbjct: 148 RSSVFYNRCKGEAEAAILSVGFPSVTVVRPSLLLGERAAFRLRERVAQTLSKAVG-GLVP 206 Query: 179 KAAKYRAVPAAKVARAMMNIALADTGGVHIYTNEVI 214 +A +R VP VA A++ A D GV + N + Sbjct: 207 QA--WRPVPVESVAAALVTAARVDAPGVRVLENPAL 240 >PA4017 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 301 Score = 125 bits (313), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 20/212 (9%), Positives = 45/212 (21%), Gaps = 11/212 (5%) Query: 9 GATGLVGSALTEDLLNGEWAEVRVLVRRPMKLRHPGLKQVIIDWERLAEYKEQFAGA-SA 67 G + L ++ + R+ RH + E + Sbjct: 12 AIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHT---EGAYQAIYVDTPGLHM 68 Query: 68 VFCCLGTTIKKAGSQEKFERVDLEYPLAAAALAKTCGVKQFLAVSSMGANVRSRAFYSRT 127 + + V+L + + A V Sbjct: 69 EEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDN 128 Query: 128 KGKLENGLGAIGFQGLHLFRPSLLLGDREESRPGERVAAIVMKALDFAMVGKAAKYRAVP 187 + + L + F + ++ E + +AAIV K L P Sbjct: 129 VQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHL-------PEATHHFP 181 Query: 188 AAKVARAMMNIALADTGGVHIYTNEVIHVIGK 219 + ++ + + Sbjct: 182 EDYITDRSQRFMASEIIREKLMRFLGAELPYS 213 >STM3268 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 222 Score = 98.6 bits (244), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 73/213 (34%), Positives = 109/213 (51%), Gaps = 19/213 (8%) Query: 6 VVLGATGLVGSALTEDLLNG-EWAEVRVLVRRPMK----LRHPGLKQVIIDWERLAEYKE 60 ++ GATGLVG L L+N + + + RRP+ + +P Q+ + LA+ + Sbjct: 16 LITGATGLVGGHLLRMLINTPQVSAIAAPTRRPLTDIVGVYNPHDPQLT---DALAQVTD 72 Query: 61 QFAGASAVFCCLGTTIKKAGSQEKFERVDLEYPLAAAALAKTCGVKQFLAVSSMGANVRS 120 VFCCLGTT ++AGS+ F D + A + G + + VS+MGAN S Sbjct: 73 PV---DIVFCCLGTTRREAGSKAAFIHADYTLVVDTALTGRRLGAQHMVVVSAMGANAHS 129 Query: 121 RAFYSRTKGKLENGLGAIGFQGLHLFRPSLLLGDREESRPGERVAAIVMKALDFAMVGKA 180 FY+R KG++E L A + L + RPS+LLGDR R E + A + + L Sbjct: 130 PFFYNRVKGEMEEALIAQNWPRLTIARPSMLLGDRTTRRVNETLFAPLFRLL-------P 182 Query: 181 AKYRAVPAAKVARAMMNIALADTG-GVHIYTNE 212 ++++ A VARAM+ ALA GV I T+ Sbjct: 183 GNWKSIDARDVARAMLAEALAPAQEGVTILTSS 215 >ZyraR [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 226 Score = 97.1 bits (240), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 67/200 (33%), Positives = 102/200 (51%), Gaps = 18/200 (9%) Query: 6 VVLGATGLVGSALTEDLLNGEWAE-VRVLVRRPMK----LRHPGLKQVIIDWERLAEYKE 60 ++ GATGLVG L L+N + RRP+ + +P Q+ + LA+ + Sbjct: 20 LITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGVFNPHDPQLS---DALAQVTD 76 Query: 61 QFAGASAVFCCLGTTIKKAGSQEKFERVDLEYPLAAAALAKTCGVKQFLAVSSMGANVRS 120 VFCCLGTT ++AGS+E F D + A + G + L VS+MGAN S Sbjct: 77 PI---DIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLGAQHMLVVSAMGANAHS 133 Query: 121 RAFYSRTKGKLENGLGAIGFQGLHLFRPSLLLGDREESRPGERVAAIVMKALDFAMVGKA 180 FY+R KG++E L A + L + RPS+LLGDR + R E + A + + L Sbjct: 134 PFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNETLFAPLFRLL-------P 186 Query: 181 AKYRAVPAAKVARAMMNIAL 200 ++++ A VAR M+ ++ Sbjct: 187 GNWKSIDARDVARVMLAESM 206 >ECs4033 [MG] COG0702 Predicted nucleoside-diphosphate-sugar epimerases Length = 226 Score = 97.1 bits (240), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 68/200 (34%), Positives = 102/200 (51%), Gaps = 18/200 (9%) Query: 6 VVLGATGLVGSALTEDLLNGEWAE-VRVLVRRPMK----LRHPGLKQVIIDWERLAEYKE 60 ++ GATGLVG L L+N + RRP+ + +P Q+ + LA+ + Sbjct: 20 LITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGVFNPHDPQLT---DALAQVTD 76 Query: 61 QFAGASAVFCCLGTTIKKAGSQEKFERVDLEYPLAAAALAKTCGVKQFLAVSSMGANVRS 120 VFCCLGTT ++AGS+E F D + A + G + L VS+MGAN S Sbjct: 77 PI---DIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLGAQHMLVVSAMGANAHS 133 Query: 121 RAFYSRTKGKLENGLGAIGFQGLHLFRPSLLLGDREESRPGERVAAIVMKALDFAMVGKA 180 FY+R KG++E L A + L + RPS+LLGDR + R E + A + + L Sbjct: 134 PFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNETLFAPLFRLL-------P 186 Query: 181 AKYRAVPAAKVARAMMNIAL 200 ++++ A VAR M+ A+ Sbjct: 187 GNWKSIDARDVARVMLAEAM 206 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,467,406 Number of extensions: 593271 Number of successful extensions: 1162 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1158 Number of HSP's successfully gapped: 11 Length of query: 220 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 118 Effective length of database: 26,614,363 Effective search space: 3140494834 Effective search space used: 3140494834 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)