BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0028.p (589 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0696 [M] COG0463 Glycosyltransferases involved in cell wall b... 120 6e-27 TM0757_1 [M] COG0463 Glycosyltransferases involved in cell wall ... 66 2e-10 RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall b... 63 1e-09 HI0653 [M] COG0463 Glycosyltransferases involved in cell wall bi... 57 6e-08 FN0542 [M] COG0463 Glycosyltransferases involved in cell wall bi... 57 8e-08 >lin0696 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 301 Score = 120 bits (301), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 0/429 (0%), Positives = 0/429 (0%), Gaps = 33/429 (7%) Query: 1 MIVKNEADNLPQCLKSVRGVADEIXXXXXXXXXXXXQIARSYGARIVNFPWSGDFXXXXX 60 + + + + D Sbjct: 9 GFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHM 68 Query: 61 XXXXXXXXQWILVLDADEELDQGSAGEL----------LLCAEHMEYEAFFLRIHNHRGT 110 + + + ++ + E Sbjct: 69 EEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREG------------K 116 Query: 111 SRSSETITVNPILRMFRNRPAYRFSGIIHEQIASVIVREKPAAAMHLSTVVIHHYGYADG 170 + + ++ + + + +A L+ I Sbjct: 117 APVILAVNKVDNVQEKADLLPHLQFLASQMNFLDI---VPISAETGLNVDTIAAIVRKHL 173 Query: 171 VVAKKDKIRRNVDLLKEQLKRSPGDAFHHFNMAVEYMRLGEYDHALEHIHVSLEHVEPDT 230 A + ++ S + + + + Sbjct: 174 PEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDING 233 Query: 231 SYVHLLHKYEI-RCLAVKGDVQGALAACERGITLFPDYPDLHHIKGVLLLQTAAWKEAKE 289 + + ++ + + + P + + A + A Sbjct: 234 LILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALR 293 Query: 290 ALRQALD---IGVSPPGYHTEAGCGTYATLSLLGQLCQETGEDYEAAAFYTKAARLHPEP 346 +L D Sbjct: 294 SLGYVDD--- 350 Query: 347 WPLIARLVRTMKCSGRESELNGWLELHLPPAAAEHRKLAVLLLNEGCYGAAAELLEKSGF 406 Sbjct: 351 - 409 Query: 407 NDALGPMGQ 415 Sbjct: 410 418 >TM0757_1 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 200 Score = 65.9 bits (159), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 55/192 (28%), Positives = 82/192 (42%), Gaps = 12/192 (6%) Query: 1 MIVKNEADNLPQCLKSVRGVADEIXXXXXXXXXXXXQIARSYGARIVNFPWSGDFXXXXX 60 MIVK+E N+ + L+S++ V DEI +I R Y ++ W DF Sbjct: 10 MIVKDEEHNIRRALESIKDVVDEIVVVDTGSTDRTPEIVREYTDKLYFHEWKNDFSEARN 69 Query: 61 XXXXXXXXQWILVLDADEELDQGSAGELLLCAEHM--EYEAFFLRIHNHRGTSRSSETIT 118 +W+L+LDADEE + + E + + +L ++ I Sbjct: 70 FSLKFPTCEWVLILDADEEASEEFRQNIRSFLESLPEDVNTVYLPTVSYLDWDFKRTEIA 129 Query: 119 VNPILRMFRNRPAYRFSGIIHEQIASVIVREKPAAAMHLSTVVIHHYGYADGVVAKKDKI 178 P R+FRN Y + I+H Q KP + VI+HYGY KK+K Sbjct: 130 STP--RIFRNGTVY-YKNIVHNQAVY-----KPKVVH--APFVIYHYGYIWTRKLKKNKY 179 Query: 179 RRNVDLLKEQLK 190 R L++EQLK Sbjct: 180 ERTATLIREQLK 191 >RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 362 Score = 63.2 bits (152), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 85/369 (23%), Positives = 141/369 (38%), Gaps = 49/369 (13%) Query: 1 MIVKNEADNLPQCLKSVRGVADEIXXXXXXXXXXXXQIARSYGARIVNFPWSGDFXXXXX 60 +I +NEA + +CL+S R D + IA + GA++ +F W DF Sbjct: 17 VIARNEAACIERCLRSARPHVDRMIVLDTGSTDATVAIAEACGAQVHHFTWVDDFSAARN 76 Query: 61 XXXXXXXXQWILVLDADEELDQGSAGEL-----------LLCAEHMEYEAFFLRIHNHRG 109 W LV+DADE L+ G AGEL ++C + ++ + G Sbjct: 77 AALQFADADWNLVMDADEWLESG-AGELREACTFGPLLGVVCIQSLD---------DAGG 126 Query: 110 TSRSSETITVNPILRMFRNRPAYRFSGIIHEQIASVIVREKPAAAMHLSTVVIHHYGYAD 169 ++ + T + R R+ G +HEQ + + R + +V H GY Sbjct: 127 ATQRTNTWIPRLLPR------GVRYQGRVHEQPVASLRRVR-------LPLVFGHDGY-- 171 Query: 170 GVVAKKDKIR-RNVDLLKEQLKRSPGDAFHHFNMAVEYMRLG-EYDHALEHIHVSLEHVE 227 V AK +K R RN LL +L P D + + + E G + A + + Sbjct: 172 -VSAKLEKKRGRNAALLHAELADRPDDPYVLYQLGKETEINGKDPAQAADLYAQAAAGAP 230 Query: 228 PDTSYVHLLHKYEIRCLAVKGDVQGALAACERGITLFPDYPDLHHIKGVLLLQTAA---- 283 + Y H + CL G + A+ + + PD + G L+ A Sbjct: 231 ANAPYRHDACIRLLLCLRQSGRLDEAITRAGEWMDTWAASPDYFFVLGHLMFDAALRHPA 290 Query: 284 -----W-KEAKEALRQALDIGVSPPGYHTEAGCGTYATLSLLGQLCQETGEDYEAAAFYT 337 W A+ A + L IG P + G G++A L + + + +A + Sbjct: 291 QAAEHWLPMAEHAFLRCLAIGDQPKLDDSVFGRGSFAAAENLAVVYRALNQPLKADEYQA 350 Query: 338 KAARLHPEP 346 + RL +P Sbjct: 351 LSQRLKADP 359 >HI0653 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 254 Score = 57.4 bits (137), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 51/181 (28%), Positives = 74/181 (40%), Gaps = 16/181 (8%) Query: 1 MIVKNEADNLPQCLKSVRGVADEIXXXXXXXXXXXXQIARSYGARIV-NFPWSGDFXXXX 59 MI+KNEA +L CL +V+ DEI +IA SYGA+ N W G F Sbjct: 8 MIIKNEAQDLANCLDTVKDWVDEIIILDSGSTDNTKEIALSYGAKFYENSDWQG-FGKQR 66 Query: 60 XXXXXXXXXQWILVLDADE----ELDQGSAGELLLCAEHMEYEAFFLRIHNHRGTSRSSE 115 ++L LDADE +L Q + E+ YE R+ G Sbjct: 67 QLAQQYVTSDYVLWLDADERVTPKLQQAILSAVKNDRENTVYE--IPRVSEVFGREIRHS 124 Query: 116 TITVNPILRMFRNRPAYRFSGIIHEQI---ASVIVREKPAAAMHLSTVVIHHY-----GY 167 + ++R++R A ++HE++ A V + H + IHHY GY Sbjct: 125 GWYPDYVVRLYRTNYAQYNDSLVHEKVEFPAGTKVEKLTGDLEHFTYKSIHHYLVKSAGY 184 Query: 168 A 168 A Sbjct: 185 A 185 >FN0542 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 263 Score = 57.0 bits (136), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 47/177 (26%), Positives = 72/177 (40%), Gaps = 23/177 (12%) Query: 1 MIVKNEADNLPQCLKSVRGVADEIXXXXXXXXXXXXQIARSYGARIVNFPWSGDFXXXXX 60 MI NE NL + LKSV+ ADEI +IA+ +GA+ V W G + Sbjct: 11 MITLNEEKNLERTLKSVQDFADEIVIVDSGSTDKTEEIAKKFGAKFVYQQWLG-YGPQRN 69 Query: 61 XXXXXXXXQWILVLDADEELDQGSAGELLLCAEHMEYEAF--------FLRIHNHRGTSR 112 WIL +DADEE+ A ++ E+ Y+ + F + H G S Sbjct: 70 RAIELSTSDWILNIDADEEISPELANKIKGIKENSRYKVYKINFMSVCFNKKIKHGGWSN 129 Query: 113 SSETITVNPILRMFRNRPAYRFSGIIHEQIASVIVREKPAAAMHLSTVVIHHYGYAD 169 + +R+FR +HE+ V + +H I+H+ Y+D Sbjct: 130 TYR-------IRLFRKNAGSYNENSVHEE----FVTNQEIVKLH---KYIYHHSYSD 172 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,630,844 Number of extensions: 1718283 Number of successful extensions: 2633 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2646 Number of HSP's successfully gapped: 7 Length of query: 589 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 477 Effective length of database: 25,461,023 Effective search space: 12144907971 Effective search space used: 12144907971 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)