BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0020.p (289 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA2147 [E] COG0346 Lactoylglutathione lyase and related lyases 249 5e-66 pli0045 [E] COG2113 ABC-type proline/glycine betaine transport s... 233 2e-61 PA3236 [E] COG2113 ABC-type proline/glycine betaine transport sy... 211 1e-54 BMEII0550 [E] COG2113 ABC-type proline/glycine betaine transport... 185 8e-47 lin1015 [E] COG2113 ABC-type proline/glycine betaine transport s... 181 1e-45 >NMA2147 [E] COG0346 Lactoylglutathione lyase and related lyases Length = 315 Score = 249 bits (635), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 126/251 (50%), Positives = 167/251 (66%), Gaps = 3/251 (1%) Query: 35 YVAWDSEIASTNVVKEVLESKLGASVEMLQVDAGPMWAGIADGSADAMVAAWLPSTHASY 94 YV WD EIASTNV+++VLE VE++ VDAGP++ G+ADG D +AWLP TH SY Sbjct: 62 YVLWDGEIASTNVIQQVLEQAGYEDVEIVAVDAGPLYQGLADGQFDFTTSAWLPYTHQSY 121 Query: 95 LEKYGQDMEDLGANLEGTKTGLAVPAYMDIKSIEDLKNAGVAKSLNNQIIGIEPGAGIMT 154 E YG ++ + NLE K GL VP+Y+ I SIE+L + N QIIGI+PGAGIM Sbjct: 122 WEAYGDQLDSVQTNLEDCKIGLVVPSYVTIDSIEELNSE--KDKFNGQIIGIDPGAGIMQ 179 Query: 155 ASQKAIDAYGLSDYTLLESSSAAMAQELQKAYTDNKPIVVTGWTPHWMFANMDLKYLDDP 214 AS+ AI Y L D L+ SSAAM L+K+ + VVT W+PHW F DLKYLDDP Sbjct: 180 ASETAITDYDL-DMELVSGSSAAMTTSLKKSIDSEEWTVVTLWSPHWAFNRWDLKYLDDP 238 Query: 215 KGVYGGAEQIHTMVRKGLKEDMPDVYRFLDQFQWTPEDMAQVMGEIQGGKSPEDAAKAWV 274 KG YG A+ + T+ R GL+E+ P++Y L +FQWT +D+ VM +I+ G +PE+AA WV Sbjct: 239 KGAYGDADHVETLARLGLEEEKPNLYGILTRFQWTHDDIQTVMMDIENGTAPEEAAANWV 298 Query: 275 SEHEDQVNTWI 285 + + ++VN WI Sbjct: 299 ANNPEKVNEWI 309 >pli0045 [E] COG2113 ABC-type proline/glycine betaine transport systems, periplasmic components Length = 289 Score = 233 bits (595), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 119/252 (47%), Positives = 165/252 (65%), Gaps = 8/252 (3%) Query: 36 VAWDSEIASTNVVKEVLESKLGASVEMLQVDAGPMWAGIADGSADAMVAAWLPSTHASYL 95 V W+SEIASTNV+ +VL+ + G +V++ VD M++ +A+G DAMV W+P+TH +Y Sbjct: 43 VNWESEIASTNVLAQVLK-EAGFNVQITTVDPAIMFSSVAEGQLDAMVGGWVPTTHQAYA 101 Query: 96 EKYGQDMEDLGANLEGTKTGLAVPAYM-DIKSIEDLKNAGVAKSLNNQIIGIEPGAGIMT 154 EKYG M DLGANLEG + L VP YM DI SI DL + ++ I IEPGAG+ Sbjct: 102 EKYGDSMVDLGANLEGAISALTVPTYMEDINSITDLTDEN-----DSTITAIEPGAGVTN 156 Query: 155 ASQKAIDAY-GLSDYTLLESSSAAMAQELQKAYTDNKPIVVTGWTPHWMFANMDLKYLDD 213 ++Q A+ Y LSD+ + SS+ AM EL +A + + IVV GW PHWMF + DLK LDD Sbjct: 157 SAQNAVKEYDNLSDWEVSVSSTGAMIAELDQAINNEEDIVVVGWKPHWMFMDYDLKMLDD 216 Query: 214 PKGVYGGAEQIHTMVRKGLKEDMPDVYRFLDQFQWTPEDMAQVMGEIQGGKSPEDAAKAW 273 P+ V+GG E+IH+ R+GLKED P+ Y+ +D F W EDM+ VM E+ PE+AA W Sbjct: 217 PENVFGGYEEIHSYAREGLKEDNPEAYKIIDNFYWEVEDMSSVMEELATDVEPEEAADNW 276 Query: 274 VSEHEDQVNTWI 285 + + + V+ W+ Sbjct: 277 IEANREIVDGWL 288 >PA3236 [E] COG2113 ABC-type proline/glycine betaine transport systems, periplasmic components Length = 285 Score = 211 bits (536), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 108/249 (43%), Positives = 149/249 (59%), Gaps = 4/249 (1%) Query: 38 WDSEIASTNVVKEVLESKLGASVEMLQVDAGPMWAGIADGSADAMVAAWLPSTHASYLEK 97 W +A+T+V EV+ KLG V+++ V AG MW G+A G DAM++AWLP TH +Y EK Sbjct: 39 WSDSVATTHVAAEVIREKLGYEVKLMPVAAGIMWQGVARGKLDAMLSAWLPGTHGAYYEK 98 Query: 98 YGQDMEDLGANLEGTKTGLAVPAYMDIKSIEDLKNAGVAKSLNNQIIGIEPGAGIMTASQ 157 + +L N + GL VP Y+ SI DL+ + +++GI+ GAG+M + Sbjct: 99 MKDKVVNLAINYPDARIGLIVPEYVSANSIADLQAQ--KDAFGGRVVGIDAGAGVMIKTD 156 Query: 158 KAIDAYGLSDYTLLESSSAAMAQELQKAYTDNKPIVVTGWTPHWMFANMDLKYLDDPKGV 217 +AI YGL DY L+ SS + M EL +A + KP+VVTGW PHWMFA LK+LDDPK V Sbjct: 157 EAIKQYGL-DYKLVASSGSGMIAELTRAENEKKPVVVTGWIPHWMFAKWKLKFLDDPKKV 215 Query: 218 YGGAEQIHTMVRKGLKEDMPDVYRFLDQFQWTP-EDMAQVMGEIQGGKSPEDAAKAWVSE 276 YG E + T+ L+ V+ FL +F W ++ QVM IQ G PEDAAK WV+ Sbjct: 216 YGDTEHVDTVANPALEAKAKPVWEFLKKFGWKDGNEVGQVMLAIQEGAKPEDAAKQWVAA 275 Query: 277 HEDQVNTWI 285 + +V W+ Sbjct: 276 NPGRVKDWV 284 >BMEII0550 [E] COG2113 ABC-type proline/glycine betaine transport systems, periplasmic components Length = 287 Score = 185 bits (469), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 100/252 (39%), Positives = 142/252 (56%), Gaps = 5/252 (1%) Query: 38 WDSEIASTNVVKEVLESKLGASVEMLQVDAGPMWAGIADGSADAMVAAWLPSTHASYLEK 97 W T + ++++E LG VE++Q D P++ G++ G DAM+ AWLP THA Y ++ Sbjct: 37 WSDAEFVTKLARKLIEDNLGQKVELVQTDVAPLYQGVSRGDIDAMMMAWLPETHADYYKR 96 Query: 98 YGQDMEDLGANLEGTKTGLAVPAYM---DIKSIEDLKNAGVAKSLNNQIIGIEPGAGIMT 154 +E+LG EG K G VP Y+ ++ SIEDLK V + L +I GI+PGAG+ Sbjct: 97 VEGKVENLGPLYEGAKLGWIVPDYIPESEVSSIEDLKKPEVREKLKGEIQGIDPGAGLTR 156 Query: 155 ASQKAIDAYGLSDYTLLESSSAAMAQELQKAYTDNKPIVVTGWTPHWMFANMDLKYLDDP 214 SQ+AI YGL DY L SS AAM + +A V T W+PHWMF L+Y+ DP Sbjct: 157 LSQEAIKKYGL-DYKLNISSEAAMLTTVDRANRSEGWFVATSWSPHWMFGKYKLRYIADP 215 Query: 215 KGVYGGAEQIHTMVRKGLKEDMPDVYRFLDQFQWTPEDMAQVMGEIQGGKSPEDAAKAWV 274 KG GGAE I + RKG KED P V L + ++ M + Q S E A ++ Sbjct: 216 KGALGGAEHIDAVARKGFKEDNPKVAALLAKMSIPINELEAAMFDAQ-ETSYEKAVDKYI 274 Query: 275 SEHEDQVNTWIA 286 ++H D++ W++ Sbjct: 275 ADHPDRIKEWLS 286 >lin1015 [E] COG2113 ABC-type proline/glycine betaine transport systems, periplasmic components Length = 300 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 86/157 (54%), Positives = 108/157 (68%), Gaps = 2/157 (1%) Query: 135 VAKSLNNQIIGIEPGAGIMTASQKAIDAYGLSD--YTLLESSSAAMAQELQKAYTDNKPI 192 + + +N I GI+ GAGIM A+Q AI Y L D + L SS+AAM LQKA D +PI Sbjct: 34 LGEQINYTITGIDAGAGIMLATQNAIKDYHLDDDNWQLQTSSTAAMTSTLQKAMKDKRPI 93 Query: 193 VVTGWTPHWMFANMDLKYLDDPKGVYGGAEQIHTMVRKGLKEDMPDVYRFLDQFQWTPED 252 VVTGWTPHWMF DLK+LDDPK V+G AE IHT+VRKGLKE+ P Y+ LD F WT ED Sbjct: 94 VVTGWTPHWMFTKFDLKFLDDPKNVFGNAENIHTIVRKGLKEEKPSAYKVLDNFFWTAED 153 Query: 253 MAQVMGEIQGGKSPEDAAKAWVSEHEDQVNTWIAGIE 289 M++VM E+ G PE+AAK W+ + ++V W G+E Sbjct: 154 MSEVMLEVNDGVDPEEAAKKWIKNNPEKVAKWTDGVE 190 Score = 103 bits (256), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 56/98 (57%), Positives = 68/98 (69%), Gaps = 2/98 (2%) Query: 35 YVAWDSEIASTNVVKEVLESKLGASVEMLQVDAGPMWAGIADGSADAMVAAWLPSTHASY 94 YVAWDSEIASTNVV E L+ ++G + ++ PMWA +A +AD MVAAWLP+T Y Sbjct: 201 YVAWDSEIASTNVVAEALK-QVGYKPTIQAMEIQPMWASVATDAADGMVAAWLPNTSGIY 259 Query: 95 LEKYGQDMEDLGANLEGTKTGLAVPAYM-DIKSIEDLK 131 + Y EDLG NL+G K GLAVP YM +I SIEDLK Sbjct: 260 YKDYKGKFEDLGPNLKGAKIGLAVPKYMTNINSIEDLK 297 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.129 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,208,821 Number of extensions: 842764 Number of successful extensions: 1315 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 1276 Number of HSP's successfully gapped: 21 Length of query: 289 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 184 Effective length of database: 26,268,361 Effective search space: 4833378424 Effective search space used: 4833378424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)