BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0019.p (277 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0549 [E] COG4176 ABC-type proline/glycine betaine transport... 169 5e-42 AGl1619 [E] COG4176 ABC-type proline/glycine betaine transport s... 164 1e-40 SMc00671 [E] COG4176 ABC-type proline/glycine betaine transport ... 161 1e-39 AGc1629 [E] COG4176 ABC-type proline/glycine betaine transport s... 155 6e-38 SMc02379 [E] COG4176 ABC-type proline/glycine betaine transport ... 155 8e-38 >BMEII0549 [E] COG4176 ABC-type proline/glycine betaine transport system, permease component Length = 301 Score = 169 bits (427), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 95/261 (36%), Positives = 126/261 (48%) Query: 17 NWLTTYLGPFFDFIHAVIGGMVNGIEXXXXXXXXXXXXXXXXXXXYWIGKWRMXXXXXXX 76 N++ + P D I A IG + +GI+ W W+ Sbjct: 16 NYILDHFTPALDMIAAAIGFVTDGIQNALIAVPPFAGVAILSLLALWRVGWKFAVFTLLA 75 Query: 77 XXXXXXXXXXXPSMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQNTVTPVLDFMQTMPA 136 +M+ IG+PLG+ A+S+ V + V P+LD +QTMPA Sbjct: 76 LALIIHMDLWSGTMESLSLVLASTVIAVVIGIPLGIAMARSDAVASIVRPILDLIQTMPA 135 Query: 137 FVYLLPAVSFFSLGVVPGVIASIIFAIPPTIRLTNLGIRQVSPELVEAADAFGSTSGQKL 196 FVYL+PA FF LG VPG IA++IFA+PP +RLTNLGIRQV E VEA AFG T+ Q L Sbjct: 136 FVYLIPAAMFFGLGAVPGTIATVIFAMPPVVRLTNLGIRQVHAEFVEAGLAFGCTARQLL 195 Query: 197 FKLQLPIALPTIMAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRAVSQAKTXXXXXXXXX 256 FK+QLP A+P+IMAG+NQT GA G+G V + + Sbjct: 196 FKVQLPNAMPSIMAGVNQTIMLSLSMVVIASMIGAGGLGNTVLTGIQRLNVGLGFEGGLA 255 Query: 257 XXXXXXLLDRLTQKLVKPKKG 277 +LDR+TQ K G Sbjct: 256 VVILAVILDRITQSFGKGGTG 276 >AGl1619 [E] COG4176 ABC-type proline/glycine betaine transport system, permease component Length = 134 Score = 164 bits (415), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 0/164 (0%), Positives = 0/164 (0%) Query: 106 IGVPLGVLCAQSNGVQNTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPP 165 Sbjct: 176 235 Query: 166 TIRLTNLGIRQVSPELVEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXX 225 Sbjct: 236 295 Query: 226 XXXXGAQGVGAYVYRAVSQAKTXXXXXXXXXXXXXXXLLDRLTQ 269 Sbjct: 296 339 >SMc00671 [E] COG4176 ABC-type proline/glycine betaine transport system, permease component Length = 285 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 84/187 (44%), Positives = 110/187 (58%) Query: 89 SMQXXXXXXXXXXXXXXIGVPLGVLCAQSNGVQNTVTPVLDFMQTMPAFVYLLPAVSFFS 148 +MQ IGVP+G+L A+S V+N PVLD MQTMP+FVYL+PA+ F Sbjct: 92 TMQTLALMLMATIVSVVIGVPMGILVAKSRVVRNITLPVLDVMQTMPSFVYLIPALMLFG 151 Query: 149 LGVVPGVIASIIFAIPPTIRLTNLGIRQVSPELVEAADAFGSTSGQKLFKLQLPIALPTI 208 LG VP ++A+II+A+PP IRLT+LGIRQV E+VEAA AFG + GQ LF ++LP+A PTI Sbjct: 152 LGKVPAILATIIYAVPPLIRLTDLGIRQVDAEVVEAATAFGGSPGQILFGVELPLATPTI 211 Query: 209 MAGINQTXXXXXXXXXXXXXXGAQGVGAYVYRAVSQAKTXXXXXXXXXXXXXXXLLDRLT 268 MAG+NQT GA+G+G V + +LDR+T Sbjct: 212 MAGLNQTIMMALSMVVVASMIGARGLGEQVLNGIQTLDVGKGLEAGIGIVILAVVLDRIT 271 Query: 269 QKLVKPK 275 Q KP+ Sbjct: 272 QGFGKPR 278 >AGc1629 [E] COG4176 ABC-type proline/glycine betaine transport system, permease component Length = 60 Score = 155 bits (392), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 0/139 (0%), Positives = 0/139 (0%) Query: 106 IGVPLGVLCAQSNGVQNTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPP 165 Sbjct: 124 183 Query: 166 TIRLTNLGIRQVSPELVEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXX 225 Sbjct: 184 243 Query: 226 XXXXGAQGVGAYVYRAVSQ 244 Sbjct: 244 262 >SMc02379 [E] COG4176 ABC-type proline/glycine betaine transport system, permease component Length = 295 Score = 155 bits (391), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 78/164 (47%), Positives = 106/164 (64%) Query: 106 IGVPLGVLCAQSNGVQNTVTPVLDFMQTMPAFVYLLPAVSFFSLGVVPGVIASIIFAIPP 165 IG+P+G++ ++S+ QN V PVLD MQTMP+FVYL+P V +G VPG+IA +I+AIPP Sbjct: 117 IGIPMGIIMSRSDRFQNIVNPVLDVMQTMPSFVYLIPVVMLLGIGKVPGLIAVVIYAIPP 176 Query: 166 TIRLTNLGIRQVSPELVEAADAFGSTSGQKLFKLQLPIALPTIMAGINQTXXXXXXXXXX 225 IRLTNLGIR V +++EAADAFGS++ QKL +Q+P+ALPTIMAGINQT Sbjct: 177 MIRLTNLGIRLVDKDVLEAADAFGSSNWQKLKNVQMPLALPTIMAGINQTIMMALAMVVI 236 Query: 226 XXXXGAQGVGAYVYRAVSQAKTXXXXXXXXXXXXXXXLLDRLTQ 269 G QG+G V +A++ + DR++Q Sbjct: 237 ASMIGVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQ 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,117,884 Number of extensions: 440195 Number of successful extensions: 2217 Number of sequences better than 1.0e-05: 77 Number of HSP's gapped: 2226 Number of HSP's successfully gapped: 78 Length of query: 277 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 172 Effective length of database: 26,268,361 Effective search space: 4518158092 Effective search space used: 4518158092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)