BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0016.p (1683 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0024 [NU] COG3539 P pilus assembly protein, pilin FimA 232 4e-60 mll2585_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 118 8e-26 AGl2532_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 106 3e-22 SMa1587_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 103 3e-21 SMb20932_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase 91 2e-17 >STM0024 [NU] COG3539 P pilus assembly protein, pilin FimA Length = 335 Score = 232 bits (591), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 0/437 (0%), Positives = 0/437 (0%), Gaps = 39/437 (8%) Query: 518 WTLVWNDEFNDGTIDPAKWTYDLGDGTAVGNPGWGNNELEWYTNEEKNVKEADGKLIITA 577 L + + G N + + Sbjct: 212 GALYSGNFNHAGQKPEGVRAKKFSVPVKCSGLDSQVNLTMRLIATPDSHV--PQAIASDN 269 Query: 578 RKEA----QGGKEYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWMLPEDYVYGSW 633 A + + Sbjct: 270 ADVGVVVETDEGNALIPNDVQSVAPFITDSAGRANITLQAYPVSTTGETPAEGAFTALAS 329 Query: 634 AASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLPGN--STIEQFHTYSLEWE 691 Sbjct: 330 - 388 Query: 692 PGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPTAET 751 Sbjct: 389 ------ 442 Query: 752 VFPQSMEIDYVRVYELTGRPYRQPVPVTLTKE------PYLEGSLPPLADGNLIYNNGFT 805 Sbjct: 443 ------- 495 Query: 806 EHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISG----RNYLKVNISSPGGNSYSIQP 861 Sbjct: 496 ----- 550 Query: 862 QAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSPTLKAELTSTLKHYETMF 921 Sbjct: 551 -- 608 Query: 922 QMKENPDIAARVEFNLG 938 Sbjct: 609 625 Score = 75.9 bits (185), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 8/171 (4%), Positives = 21/171 (12%), Gaps = 29/171 (16%) Query: 791 PPLADGNLIYNNGFTEHKEGDTGMGIPNTAHWVLYKESGADAAVSLEPISGRNYLKVNIS 850 L G L K + + + Sbjct: 40 TTLTAEQN------------QVG------KTVQLEKSQEVNVQAVCP----AGASTYSQT 77 Query: 851 SPGGNSYSIQP-QAIVSLAKGRFYKLSFDAKTDHSRNIGVRLTGGESRGYQAYSP----- 904 S + + G + Sbjct: 78 YRSYVSPYPVVETSGNWKYLKLDPDYLEGGMRIEDSSAGDIYPPMNNVLMGYDENVKAGQ 137 Query: 905 -TLKAELTSTLKHYETMFQMKENPDIAARVEFNLGTNASPVWLGNARLEEI 954 + + + + A+ L + + Sbjct: 138 PFYVRDSNLEFQLKIVKPFVGTVNISPKTMFNVYVMTAAGDPLTDVVYSIL 188 Score = 53.9 bits (128), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 0/126 (0%), Positives = 0/126 (0%), Gaps = 14/126 (11%) Query: 1555 AQDGELAVALTGTGTANYSAQVDYANLKLVQGKTYTLAFAARSDVDRLIEVAVEHKGGDY 1614 Sbjct: 529 - 587 Query: 1615 TKYLPAKAVALTGSMNTYSYTFTMDSGTDAGAHLVFLLGLVAGNSEGTNSAIQAGNHIYI 1674 Sbjct: 588 ------------- 634 Query: 1675 DNVSLT 1680 Sbjct: 635 640 >mll2585_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 118 bits (296), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 21/268 (7%), Positives = 42/268 (15%), Gaps = 40/268 (14%) Query: 520 LVWNDEFNDGTIDPAK---WTYDLGDGTAVGNPGWGNNELEWY-------TNEEKNVKEA 569 + G + G N ++ E + + E Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 570 DGKLIITARKEAQGGK------EYTSSRIKTKDLFSKKYGKFEIRAKAPAGKGYWPAIWM 623 + + + + K K A + K Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 624 LPEDYVYGSWAASGEIDIMEGWGSRPGTVAGTIHYGSQWPDNVYSGKEYVLPGNSTIEQF 683 L + + G A + D + + EY G Sbjct: 128 LYQV---HWPQR--PTNCFGKLGYSWTDSAPAVSLL----DTLDALAEYQRAGKIRYIGV 178 Query: 684 HTYSLEWEPGELRWYVDGVLYSTKNDWYSLSNGQPANNAYPAPFNQEFHLLMNLAVGGNF 743 + L P P++ Sbjct: 179 SNETAFGVMRYLHLADKHD--------------LPRIVTIQNPYSLLNRSFEVGLAEV-S 223 Query: 744 DGNPTAETVFPQSMEIDYVRVYELTGRP 771 + Y +P Sbjct: 224 QYEGVELLAYSCLGFGTLTGKYLNGAKP 251 >AGl2532_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 106 bits (265), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 14/234 (5%), Positives = 28/234 (11%), Gaps = 44/234 (18%) Query: 552 GNNELEWYTN-------EEKNVKEADGKLIITARKEAQG------GKEYTSSRIKTKDLF 598 N + LI A G T Sbjct: 18 LGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHG 77 Query: 599 SKKYGKFEIRAKAPAGKGYWPAIWMLPEDYVYGSWAASGEIDIMEGWGSRPGTVAGTIHY 658 S++ + P+ D E + + + Sbjct: 78 SREKLIIASKVSGPSRNNDKGIRPDQALD---RKNIR--EALHDSLKRLQTDYLDLYQVH 132 Query: 659 GSQWPDNVYSGKEYVLPGNSTI---EQFHTYSLEWEPGELRWYVDGVLYSTKNDWYSLSN 715 L+ + G + Sbjct: 133 WP-------QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR----------- 174 Query: 716 GQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPTAETVFPQS-MEIDYVRVYELT 768 L T + + + Sbjct: 175 ---YIGVSNETAFGVMRYLHLADKHD-LPRIVTIQNPYSLLNRSFEVGLAEVSQ 224 >SMa1587_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 211 Score = 103 bits (257), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 0/232 (0%), Positives = 0/232 (0%), Gaps = 43/232 (18%) Query: 552 GNNELEWYTNEEKNVKEA-------DGKLIITARKEAQG------GKEYTSSRIKTKDLF 598 L + K + + Y S Sbjct: 34 IAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTC 93 Query: 599 SKKYGKFEIRAKAPAGKGYWPAIWMLPEDYVYGSWAASGEIDIMEGWGSRPGTVAGTIHY 658 + Sbjct: 94 RILLEQHNGEVPEDRAALEALPGVGRKTA---NVVLN--TAFGWPTIAVDTHIFRVCNRT 148 Query: 659 GSQWPDNVYSGKEYVLPGNSTIEQ---FHTYSLEWEPGELRWYVDGVLYSTKNDWYSLSN 715 + + V + + L Sbjct: 149 QF-------APGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYT--------------C 187 Query: 716 GQPANNAYPAPFNQEFHLLMNLAVGGNFDGNPTAETVFPQSMEIDYVRVYEL 767 Sbjct: 188 - 238 >SMb20932_2 [G] COG2273 Beta-glucanase/Beta-glucan synthetase Length = 346 Score = 90.9 bits (224), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 8/231 (3%), Positives = 25/231 (10%), Gaps = 43/231 (18%) Query: 553 NNELEWYTNEEKNVKEA-------DGKLIITARKEAQG------GKEYTSSRIKTKDLFS 599 + +T ++ + + + Sbjct: 35 QLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRN 94 Query: 600 KKYGKFEIRAKAPAGKGYWPAIWMLPEDYVYGSWAASGEIDIMEGWGSRPGTVAGTIHYG 659 G +A + + + + Sbjct: 95 NDKGIRPDQALDRKNIREALHDSLKRLQ---TDYLD--LYQVHWPQRPTNCFGKLGYSWT 149 Query: 660 SQWPDNVYSGKEYVLPGNSTIEQ---FHTYSLEWEPGELRWYVDGVLYSTKNDWYSLSNG 716 S L + Sbjct: 150 D-------SAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV--------------MR 188 Query: 717 QPANNAYPAPFNQEFHLLMNLAVGGNFDGNPTAETVFPQSMEIDYVRVYEL 767 + AE + +E+ Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNR-SFEVGLAEVSQYEGVELLAYSCLGF 238 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 117,844,955 Number of extensions: 5058185 Number of successful extensions: 7868 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 7849 Number of HSP's successfully gapped: 8 Length of query: 1683 Length of database: 38,378,431 Length adjustment: 120 Effective length of query: 1563 Effective length of database: 24,538,351 Effective search space: 38353442613 Effective search space used: 38353442613 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 123 (52.0 bits)