BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0003.p (518 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 367 e-101 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 290 4e-78 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 259 8e-69 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 83 9e-16 BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 76 1e-13 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 367 bits (941), Expect = e-101, Method: Compositional matrix adjust. Identities = 194/463 (41%), Positives = 288/463 (62%), Gaps = 15/463 (3%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M+ L + P DT+ E+E+LTNT L + P E+++N++ A+G+LP+V L++ T Sbjct: 57 MVILYHQQPPKDTVIKEIEELTNTNLDLIWVPDAVKEDRVNAALASGNLPKVITLQDFRT 116 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 + + + GQFWEIGP L EFPNL+++ IL N VDGK+Y +Y RPL+RQG++ R Sbjct: 117 SSVLN-AFRSGQFWEIGPYLDEFPNLSEMNETILENVSVDGKIYGIYRERPLSRQGVVIR 175 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 +DW + LGL+ P N++EL+ +A+AFT DP+G+G DT G DRN+L++GAFKT++S+ G Sbjct: 176 QDWLENLGLEMPTNLEELYEVARAFTYDDPNGSGADDTVGFTDRNDLIFGAFKTLASYHG 235 Query: 242 TPNSWG-EKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAG 300 TPN WG + +G+L PEF +Y +TM++ K D G +N +F TSK +F++G+AG Sbjct: 236 TPNEWGFDDEGKLMPEFMTDEYVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAG 295 Query: 301 MYIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKD 360 +Y+ G+M D L P + + +AGPDG+ W G+N + PKS+V+ Sbjct: 296 IYV-GNMVDARNLRDKAKDINPEMEVAITNRIAGPDGEDRVWAQGGHNGIFAIPKSSVET 354 Query: 361 EAELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVI 420 EAELK+ILAFFD++ +V NLM +GIE HY +DG+ E IE + + V Sbjct: 355 EAELKRILAFFDRIAEEDVYNLMAYGIEDVHYETIDGEY-----FERIEENLDDWAAEV- 408 Query: 421 GEAETNGMYQSLNVL-----PGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQI 475 + + + + +N L P E L +N ++ V +P L S TYT +G ELQ+I Sbjct: 409 -QPLVSLVAREMNYLKAYGDPLYEEYERLTEDNNEIIVTNPAEPLFSPTYTERGTELQKI 467 Query: 476 ISDATYKYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYKK 518 I DATYKYI G++D+ GFE EV+ W GG+ IIEE N Y++ Sbjct: 468 IDDATYKYILGELDEDGFEAEVERWLTNGGESIIEELNEDYER 510 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 290 bits (742), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 170/412 (41%), Positives = 240/412 (58%), Gaps = 32/412 (7%) Query: 62 MLPLNTTETPPDTIKTEVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTT 121 M L + P D E+EKLTNT+L + P E++LN++ A G+LPQ+ +++ Sbjct: 37 MAILYHQQPPKDRAIKEIEKLTNTELDITWVPDAVKEDRLNAALAAGNLPQIVTIQDIKN 96 Query: 122 FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGIIYR 181 M + + G FWEIG + ++PNLNK+ I N +DGKLY +Y RPL+RQGI+ R Sbjct: 97 SSVM-NAFRSGMFWEIGDYIKDYPNLNKMNKLINKNVTIDGKLYGIYRERPLSRQGIVIR 155 Query: 182 KDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFG 241 KDW D L L+ P +DEL+ +AKAFTE DPD +GK DT G+ DRN+L+YGAFKT+ S+ G Sbjct: 156 KDWLDNLNLKTPKTLDELYEVAKAFTEDDPDKDGKDDTFGLADRNDLIYGAFKTIGSYEG 215 Query: 242 TPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFVSGKAGM 301 P W E G+ P+F +Y DTM++ KK RD GYMN+DF TSKT +F GKAG+ Sbjct: 216 MPTDWKESGGKFTPDFMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKAGI 275 Query: 302 YIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAVKDE 361 YIG +D +N + + L+ + + GPDGK W G+N V FPK++VK E Sbjct: 276 YIGNM---VDAVNLRDHASDKSMKLEIINRIKGPDGKERVWASGGHNGVFAFPKTSVKTE 332 Query: 362 AELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDGKAKPADDKELIEREVKGFKDSVIG 421 AELK+ILAFFD++ +V +LM +GI+G HY +DK +E S + Sbjct: 333 AELKRILAFFDRIAEEDVYSLMTYGIDGVHYN-------KGEDKTFTRKE------SQVK 379 Query: 422 EAETNGMYQSLNVL-------------PGRIHAEELLLENVKVGVADPTAAL 460 + +T+ Q L+ L P R EEL +N K+ V++P + Sbjct: 380 DWQTD--IQPLSALIAIDKAYLKNTGDPLRTAYEELTEDNEKIIVSNPAESC 429 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 259 bits (662), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 161/480 (33%), Positives = 261/480 (54%), Gaps = 45/480 (9%) Query: 62 MLPLNTTETPPDTIKT--EVEKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQ 119 M PL + E+P D + +EK T ++ + P +Y +K N A+G +P +K++ Sbjct: 41 MAPLLSPESPSDKSPSLKALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAIVIKDK 100 Query: 120 TT-FIQMKESMKDGQFWEIGPLLSEFPNLNKLKPEILNNTKVDGKLYTLYIGRPLARQGI 178 + FI+ S+K G FWE+ P L ++ NL++ +IL N+ V+G++Y +Y R L R + Sbjct: 101 SAGFIK---SVKAGAFWELSPYLKDYKNLSQADEKILKNSSVNGEVYGIYRTRDLIRACM 157 Query: 179 IYRKDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYG---AFKT 235 I R DW +GL P +D+ + + KAF E+DPDGNGK DT G+V + G + Sbjct: 158 IIRTDWLKNVGLDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNGSPWDV 217 Query: 236 VSSWFGTPNSWGEKDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATSKTDAVNMFV 295 + WFG PN +G ++G+L P+F +Y D + FFKK D G +N+DFA + V Sbjct: 218 LQIWFGAPNRYGVENGKLIPDFTTKEYMDALTFFKKLYDEGLINKDFAVMDSAKWNDPVV 277 Query: 296 SGKAGMY---------IGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPG 346 GKAG+ I +M++ D NKD ++D + GP+GK + G Sbjct: 278 KGKAGVIVDTGSRASQIQSAMEEADESNKD--------IIDIVGSLEGPNGK-RTFPTSG 328 Query: 347 YNNVVLFPKSAVKDEAELKKILAFFDKMMTPEVANLMYWGIEGTHYTVVDG--KAKPADD 404 Y+ ++ PKS+VK E ELK++L+F DKM E L G++G +Y + DG + ++ Sbjct: 329 YSGMITIPKSSVKTEKELKEVLSFLDKMNDKEAQILTNNGVKGRNYELKDGVFTSLEKNN 388 Query: 405 KELI-ERE-VKGFKDSVIG-----EAETNGMYQSLNVLPGRIHAEELLLENVKVGVADPT 457 K L+ E E + F S+ E + ++Q H ++++ E K+ V +P Sbjct: 389 KSLLYEHEGLAQFSMSIPKSEYYIEDQKTKLFQ---------HRKDIITEGEKIAVFNPA 439 Query: 458 AALDSATYTSKGVELQQIISDATYKYIYGQIDKAGFEKEVQAWKERGGQKIIEEYNAVYK 517 +L S YT KG +L II DA ++I G+ID+ GF+ V+ WK+ GG +++++ N +Y+ Sbjct: 440 ESLVSDVYTQKGAQLDNIILDARTQFIIGEIDEKGFDDAVELWKKSGGNELMKDLNKLYQ 499 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 83.2 bits (204), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 88/320 (27%), Positives = 137/320 (42%), Gaps = 59/320 (18%) Query: 80 EKLTNTKLTYQFYPADTYEEKLNSSFATGSLPQVTYLKNQTTFIQMKESMKDGQFWEIGP 139 EK T K+ Y F +E++L + A +LP + + +++ GQF ++G Sbjct: 56 EKKTGIKIEY-FNATTDFEQRLARAAAGNALPDLIF----------NDAVAIGQFVQLG- 103 Query: 140 LLSEFPNLNKLKPEILNNTK------VDGKLYTLYIGRPLARQ--GIIYRKDWADKLGLQ 191 ++ N + E + +T +DGK Y G P + Q + RKDW +KLGL+ Sbjct: 104 IVEPIEPKNIIGGENIFDTAWKSTQYIDGKYY----GVPTSAQTFALFVRKDWREKLGLE 159 Query: 192 PPANVDELFAMAKAFTEQDPDGNGKKDTTGIVDRNELVYGAFKTVSSWFGTPNSWG---- 247 P N ++ +AKAFT DPDGNGK DT G + G +SWF + W Sbjct: 160 QPKNWQDISTLAKAFTFNDPDGNGKNDTYGFILPGSTTRG----YASWFISSYLWQAGGD 215 Query: 248 ----EKDGQLAPEFEFPQYYDTMDFFK-----KARDAGYMNQDFAATSKTDAVNMFVSGK 298 +G+ E P +T+ F + K G +N + DA+ F SG+ Sbjct: 216 FIRPAGEGKFIASLEEPAATETLSFMRGMVCDKTVQPGAIN-----ATTADAIPSFRSGQ 270 Query: 299 AGMYIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPGYNNVVLFPKSAV 358 +GM+ G I +KD P + ++ P G Q + V KS+ Sbjct: 271 SGMFFSGPYH-IALFDKD-----PGT--EAFEVIPPPTGPKGQATL-AEGTTVFMMKSSQ 321 Query: 359 KDEAELKKILAFFDKMMTPE 378 K A K F + M++PE Sbjct: 322 KKAAAQK----FIEFMISPE 337 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 76.3 bits (186), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 94/387 (24%), Positives = 152/387 (39%), Gaps = 67/387 (17%) Query: 181 RKDWADKLGLQPPANVDELFAMAKAFTEQDPDGNGKKDTTGI-------------VDRNE 227 R+DW DK L+PP ++EL + F +DP+ GK DT GI + + Sbjct: 179 RQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGFRGGNIADSS 238 Query: 228 LVYGAFKTVSSWFGTPNSWGE-KDGQLAPEFEFPQYYDTMDFFKKARDAGYMNQDFAATS 286 V+GAF P +W + DG L P D + + + GY+ DFA S Sbjct: 239 WVFGAFGDF-----VPGAWSKGDDGSLVYGSIQPSMKDGLAKIHEWYEKGYLATDFAILS 293 Query: 287 KTDAVNMFVSGKAGMYIGGSMQDIDTLNKDLVKNYPNAVLDTHSMVAGPDGKFAQWMIPG 346 DA F+SG++G+ + + + + P+A++ V+GP+G+ + G Sbjct: 294 AGDAQESFISGRSGIMAAPPWAHGYPIGE-MAQVVPDAIVKPFPHVSGPNGQAGR---RG 349 Query: 347 YNNVV---LFPKSAVKDEAELKKILAFFDKMMT---PEVANLMYWGIEGTHYTVVDGKAK 400 VV LF KD + K ++D + E Y EG Y + DG + Sbjct: 350 EGYVVGSFLFN----KDFEHMDKFFEYWDAIYGYTFGESDYFEYGLFEGYDYVMEDG--E 403 Query: 401 PADDKELIEREVKGFKDSVIGEAE----TN------GMYQSLNVL--------------- 435 P D E E+ G + V+ TN MY L Sbjct: 404 PVYDHAQFE-EITG--EEVVDPGRYFLPTNVPTIPFEMYSLLEEFHETGREPETGYEAGL 460 Query: 436 ----PGRIHAEELLLENVKVGVADPTAALDSATYTSKGVELQQIISDATYKYIYGQIDKA 491 P I A ++ + + + + T S+ L+++ + IYG Sbjct: 461 AGRDPDYIEAAAIVNRQNDLRIINEFNGPPTPTMVSRSEHLERMEDELFVDIIYGNKPLD 520 Query: 492 GFEKEVQAWKERGGQKIIEEYNAVYKK 518 F++ V+ W++ GG+ I EE N Y+ Sbjct: 521 AFDQFVEEWRQSGGEAITEEVNEWYES 547 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,192,649 Number of extensions: 1714266 Number of successful extensions: 2271 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2263 Number of HSP's successfully gapped: 6 Length of query: 518 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 407 Effective length of database: 25,576,357 Effective search space: 10409577299 Effective search space used: 10409577299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)