BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0028.p (380 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0275 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma... 230 3e-60 STM0262 [QR] COG0500 SAM-dependent methyltransferases 150 3e-36 alr2010 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA h... 150 4e-36 alr7569 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA h... 132 9e-31 FN0536 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA ho... 78 2e-14 >ML0275 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 380 Score = 230 bits (586), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 134/382 (35%), Positives = 225/382 (58%), Gaps = 8/382 (2%) Query: 1 MKISILKNELNESIGHVSKAISSRTTIPILTGIKFEVSHQGVTLTASDTDISIQSFIPAE 60 +K SI KN ++ +AI S+ TIP LT +K EV G+TL S+ ISI++F+P + Sbjct: 2 IKFSINKNAFQNALRITKQAIGSKVTIPALTKLKIEVEENGITLIGSNGQISIKNFLPVD 61 Query: 61 DDSQTIVKVEQPGSVVLPAKFFVEIIKKLPSKEIHMEVKEGFQTYISSGSTEIQIVGLDP 120 + +++ + GSV+L A FF ++ +LP + KE Q ++SG +EI + GLD Sbjct: 62 NKDASML-ISGTGSVLLEAAFFENVVSQLPEVTLEFTEKEQKQVLLTSGKSEITLKGLDS 120 Query: 121 EEFPVLPSIEENDTVSLPGDLLKNMIKQTAFSISTQETTPILTGI-LWNLSDNEFKFTAT 179 E +P L I E ++ + +LK + +T F++STQE PI TG+ L LS E K AT Sbjct: 121 EIYPHLQEISEGSSLKMKVKVLKEIFTETVFAVSTQENRPIFTGVHLETLSTGELKAVAT 180 Query: 180 DRHRLATRAAHLEGTESVQFANVVIAGKTLNELSKIIPDQNMLVDIVVADNQVLFKIDKV 239 D HR++ R LE +E ++F +V++ K++N + + ++I ++ +Q+LF+ + + Sbjct: 181 DSHRMSQRLLPLEDSE-LKF-DVILPSKSINSFKNVFTNDEEEIEIFISGSQMLFRNETI 238 Query: 240 LFYTRILDGIYPDTSRIIPTA--YKTELTLDTKKLSESIDRAYLLSREEKTNIVRMQTLE 297 +Y+R+++G YPDT+R+IP Y +L D +L ++DRA LL+ V++ T+ Sbjct: 239 SYYSRLIEGSYPDTNRLIPNEADYTLDLVFDAAQLRHTMDRARLLTVMTTNGTVKL-TVS 297 Query: 298 NGDVEISSSSSELGKVREELEVIDFKGEPLKISFNSKYMLDVLKVVESEQLVIAFTGMMS 357 V +++S E+G V EEL + +G L ISFN +Y++D LKV+++ ++ I F + Sbjct: 298 GDSVVTTANSPEVGSVHEELTALSKEGNDLAISFNPEYLIDALKVIKAPEVRIRFISNVR 357 Query: 358 PIILKPLD-ESRSLYVILPYRT 378 P L+P + ES + +I P RT Sbjct: 358 PFTLQPRNEESGFVQLITPVRT 379 >STM0262 [QR] COG0500 SAM-dependent methyltransferases Length = 240 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 0/378 (0%), Positives = 0/378 (0%), Gaps = 15/378 (3%) Query: 1 MKISILKNELNESIGHVSKAISSRTTIPILTGIKFEVSHQGVTLTASDTDISIQSFIPAE 60 MK + L + + + + + ++S + A Sbjct: 1 MKPARLSQTVVAPGCWGELPWGNYYREALEQQLNPWFAKMYGFHLLKIGNLSAEINSEA- 59 Query: 61 DDSQTIVKVEQPGSVVLPAKFFVEIIKKLPSKEIHMEVKEGFQTYISSGSTEIQIVGLDP 120 V +V + L V + + L Sbjct: 60 ------CAVSHQVNVSSQGSPMQVLADPLHLPFADKSV-DVCLLAHTLPWCTDPHRLLRE 112 Query: 121 EEFPVLPSIEENDTVSLPGDLLKNMIKQTAFSISTQETTPILTGILWNLSDNEFKFTATD 180 + ++ + P L+ + + + T + + F Sbjct: 113 ADRVLIDDGWLVISGFNPLSLMGLRKLVPVLRKTPPYNSRMFTLMRQLDWLSLLNFEVLH 172 Query: 181 RHRLATRAAHLEGTESVQFANVVIAGKTLNELSKIIPDQNMLVDIVVADN-QVLFKIDKV 239 R +G + I +L L + N V Sbjct: 173 YSRFHVLPWKKQGGRLLNT---HIPALGCLQLIVARKRTIPLTLNPLRHNKSKTPIRQTV 229 Query: 240 LFYTRILDGIYPDTSRIIPTAYKTELTLDTKKLSESIDRAYLLSREEKTNIVRMQTLENG 299 Sbjct: 230 - 288 Query: 300 DVEISSSSSELGKVREELEVIDFKGEPLKISFNSKYMLDVLKVVESEQLVIAFTGMMSPI 359 Sbjct: 289 -- 346 Query: 360 ILKPLDESRSLYVILPYR 377 Sbjct: 347 364 >alr2010 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA homolog) Length = 387 Score = 150 bits (379), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 106/400 (26%), Positives = 203/400 (50%), Gaps = 33/400 (8%) Query: 1 MKISILKNELNESIGHVSKAISSRTTIPILTGIKFEVSHQ--GVTLTASDTDISIQSFIP 58 MK+ +++L+ ++ V++A+ SR T P+L + + + V+LTA D + I++ Sbjct: 1 MKLVCAQSDLSTNLSLVNRAVPSRPTHPVLANVLLQADAETNQVSLTAFDLSLGIRTSFN 60 Query: 59 AEDDSQTIVKVEQPGSVVLPAKFFVEIIKKLPSKEIHME---------VKEGFQTYISSG 109 A+ V Q G++ LPAK V+I +LP EI ++ EG ++ Sbjct: 61 AD--------VWQSGAIALPAKLLVDITSRLPEGEITLDDESATDGTATGEGLIVSLTPK 112 Query: 110 STEIQIVGLDPEEFPVLPSIEENDTVSLPGDLLKNMIKQTAFSISTQETTPILTGILWNL 169 + + Q+ + EFP LP IE + L L ++ + F+ S ET +LTG+ + Sbjct: 113 TGKYQLRAMGAGEFPELPLIENTTAIYLTATSLIEGLRGSLFATSGDETKQVLTGVHLTV 172 Query: 170 SDNEFKFTATDRHRLA----TRAAHLEGTESVQFANVVIAGKTLNELSKIIPDQNML--- 222 + +F ATD HRLA T LE + Q V + + L EL +++ Sbjct: 173 KQDTLEFAATDGHRLAVVETTNERPLEDND--QQLEVTVPARALRELERMLAHNAASEEP 230 Query: 223 VDIVVADNQVLFKIDKVLFYTRILDGIYPDTSRIIPTAYKTELTLDTKKLSESIDRAYLL 282 + + + QV+F +R L+G YP ++IP ++ ++T++ ++ +++R +L Sbjct: 231 IALYLDQGQVVFAWQNQRLTSRTLEGQYPAYRQLIPRQFERQVTIERRQFLSTLERIAVL 290 Query: 283 SREEKTNIVRMQTLENGDVE--ISSSSSELGKVREELEVIDFKGEPLKISFNSKYMLDVL 340 + ++K NIV++ T+++ E +S + E+G RE + + GE ++I+FN KY+++ L Sbjct: 291 A-DQKNNIVKL-TIDSTAQELTLSCEAQEMGSGRESMNA-EIAGEDIEIAFNVKYLMEGL 347 Query: 341 KVVESEQLVIAFTGMMSPIILKPLDESRSLYVILPYRTTN 380 K + S ++ + ++P+I PL + Y+ +P + N Sbjct: 348 KALPSPEIQMHINQNLTPVIFTPLGGLKMTYLAMPVQLRN 387 >alr7569 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA homolog) Length = 359 Score = 132 bits (332), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 0/385 (0%), Positives = 0/385 (0%), Gaps = 25/385 (6%) Query: 1 MKISILKNELNESIGHVSKAISSRTTIPILTGIKFEVSHQG-VTLTASDTDISIQSFIPA 59 M+ + + ++ + ++ +K G + + D Sbjct: 1 MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFG---- 56 Query: 60 EDDSQTIVKVEQPGSVVLPAKFFVEIIKKLPSKEIHMEVKEGFQTYISSGSTEIQIVGLD 119 ++ K + + + + + + E + Sbjct: 57 ----VARLEEALRLRAGGITKPVLLLEGFF-DARDLPTI-SAQHFHTAVHNEEQLAALEE 110 Query: 120 PEEFPVLPSIEENDTVSLPGDLLKNMIKQTAFSISTQETTPILTGILWNLSDNEFKFTAT 179 + + DT + + ++ + I+ + + + Sbjct: 111 ASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGA 170 Query: 180 DRHRLATRAAHL--EGTESVQFANVVIAGKTLNELSKIIPDQ--NMLVDIVVADNQVLFK 235 +LA + + G L S + +L + +++ Sbjct: 171 TEKQLAIFNTFCEGKPGQRSIA---ASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGA 227 Query: 236 IDKVLFYTRILDGIYPDTSRIIPTAYKTELTLDTKKLSESIDR-AYLLSREEKTNIVR-M 293 + + T +++ + + Sbjct: 228 DFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPR--AAPSG 285 Query: 294 QTLENGDVEISSSSSELGKVREE--LEVIDFKGEPLKISFNSKYMLDVLKVVESEQLVIA 351 + E+ + K I + ++ + + Sbjct: 286 TPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYEL 345 Query: 352 FTGMMSPIILKPLDE-SRSLYVILP 375 Sbjct: 346 370 >FN0536 [L] COG0592 DNA polymerase sliding clamp subunit (PCNA homolog) Length = 381 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 90/390 (23%), Positives = 178/390 (45%), Gaps = 29/390 (7%) Query: 1 MKISILKNELNESIGHVSKAISSRTTIPILTGIKFEVSHQGVTLTASDTDISIQSFIPAE 60 M I + + +I V K++ PIL+ I +V + T ++ D +I++ I Sbjct: 1 MHIKVNRQNFLSAIRIVEKSVKENKIKPILSCIYAKVKGNKIYFTGTNLDTTIKTSIDVN 60 Query: 61 DDSQTIVKVEQPGSVVLPAKFFVEIIKKLPSKEIHMEVKEGFQTYISS--GSTEIQIVGL 118 + V + G V E +K++ + + + V+ G +I + +TE + Sbjct: 61 E-------VIREGEVAFYYSIIDEYLKEIKDEFVVLRVENGNILFIETEDSTTEYDVFSA 113 Query: 119 D--PEEFPVLPSIEENDTVSLPGDLLKNMIKQTAFSISTQETTPI----LTGILWNLSDN 172 + P F + E N +P L N+ ++ FS T + + + IL +L Sbjct: 114 EDYPNTFENIVLNENNFKFEMPSQELVNIFEKVLFSADTPDNIAMNCIRIESILKHLH-- 171 Query: 173 EFKFTATDRHRLATRAAHLEGTESVQFANVVIAGKTLNELSKIIPDQNMLVDIVVADNQV 232 F +T+ +RL +++ S F+ V A + + I N ++ I D + Sbjct: 172 ---FVSTNTYRLTFLKKNIDKDIS-DFSVSVPADTISSIIKIIKGLDNEVIKIYKEDAHL 227 Query: 233 LFKIDKVLFYTRILDGIYPDTSRIIPT-AYKTELTLDTKKLSESIDRAYLLSR---EEKT 288 F+ + T++++ +P+ + I+ +Y +L ++ KL+ + R + SR E K Sbjct: 228 YFQYKDTMIITKLIELRFPNYAEILSNISYDKKLHMNNDKLTNLLKRILIFSRSNSESKY 287 Query: 289 NIVR--MQTLENGDVEISSSSSELGKVREELEVIDFKGEPLKISFNSKYMLDVLKVVESE 346 + EN + S+ +E+ ++ EEL+V +F+GE LKIS NSKY+L+ ++ + E Sbjct: 288 SSTYEFKHNEENKNKMAISALNEIARINEELDV-NFEGEDLKISLNSKYLLEFIQNIPKE 346 Query: 347 -QLVIAFTGMMSPIILKPLDESRSLYVILP 375 +LV+ F S + + D +Y+++P Sbjct: 347 KELVLEFMYSNSAVKVYEKDNDEYIYILMP 376 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,164,492 Number of extensions: 959721 Number of successful extensions: 1580 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1563 Number of HSP's successfully gapped: 6 Length of query: 380 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 272 Effective length of database: 25,922,359 Effective search space: 7050881648 Effective search space used: 7050881648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)