BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0021.p (628 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value FN1723 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved ... 584 e-166 FN0007 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved ... 521 e-147 DR0027 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved ... 434 e-121 >FN1723 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved in cell division Length = 176 Score = 584 bits (1506), Expect = e-166, Method: Compositional matrix adjust. Identities = 0/627 (0%), Positives = 0/627 (0%), Gaps = 17/627 (2%) Query: 8 YDVVVIXXXXXXXXXXXXXXXXXCRTLMITINLDMVAFMPCNPSIGGPAKGHVVREIDAL 67 +V F+ S + L Sbjct: 4 LNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTL 63 Query: 68 GGEMGRNIDKTFIQLRMLNTGKGPAVHALRAQADKFLYQHAMKETMENTPNLTLRQGMVE 127 + + + + A + ++ + + Sbjct: 64 SLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKD 123 Query: 128 ELIVEN----GRCAGVITKTGTVYHSKTVILTTGTYLRGKVIMGELTYESGPNNQQPSVR 183 + Sbjct: 124 VNDLPELLKA 183 Query: 184 LSENLRELGFDLVRFKTGTPPRVHKDTIDFSKTEIQPGDEKPKFFSFETKSSDNE----- 238 Sbjct: 184 - 242 Query: 239 QLPCWLTYTSEVTHQIINDNLHRAPMFTGIIEGTGPRYCPSIEDKVVRFSDKSQHQIFLE 298 Sbjct: 243 302 Query: 299 PEGKNTAEYYVQGLSTSLPEDVQLAILRSIPGMEKVEMMRNGYAIEYDAMVPTQLWPSLE 358 Sbjct: 303 362 Query: 359 TKRLPGLFTAGQINGTSGYEEXXXXXXXXXXXXXRKVQEKEPVVLDRSQGYIGVLIDDLV 418 Sbjct: 363 422 Query: 419 TKGTNEPYRLLTSRAEYRLLLRHDNADLRLTPIGYEIGLIPKQRYEAFLDKKXXXXXXXX 478 Sbjct: 423 482 Query: 479 XXXXTKVKPV--EVNAALAEYESAPIVDGSNLLTLMRRPEVAYSFVDLVSPSPEGL---- 532 Sbjct: 483 542 Query: 533 -DEEMKEQVEIQIKYAGYIEKQLLHVEKLQKMEKKKIPDDINYNEIHGLAMEARQKLTKI 591 Sbjct: 543 602 Query: 592 APISIGQASRIAGVTPADISILLVHLE 618 Sbjct: 603 629 >FN0007 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved in cell division Length = 176 Score = 521 bits (1343), Expect = e-147, Method: Compositional matrix adjust. Identities = 0/628 (0%), Positives = 0/628 (0%), Gaps = 26/628 (4%) Query: 8 YDVVVIXXXXXXXXXXXXXXXXXCRTLMITINLDMVAFMPCNPSIGGPAKGHVVREIDAL 67 I + + + Sbjct: 5 NVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLS 64 Query: 68 GGEMGRNIDKTFIQLRMLNTGKGPAVHALRAQADKFLYQHAMKETMENTPNLTLRQGMVE 127 ++ + + D+ + + + Sbjct: 65 LDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDV 124 Query: 128 ELIVEN----------GRCAGVITKTGTVYHSKTVILTTGTYLRGKVIMGELTYESGPNN 177 + E Sbjct: 125 NDLPELLKAQIAHFFE 184 Query: 178 QQPSVRLSENLRELGFDLVRFKTGTPPRVHKDTIDFSKTEIQPGDEKPKFFSFETKSSDN 237 Sbjct: 185 244 Query: 238 EQLPCWLTYTSEVTHQIINDNLHRAPMFTGIIEGTGPRYCPSIEDKVVRFSDKSQHQIFL 297 Sbjct: 245 304 Query: 298 EPEGKNTAEYYVQGLSTSLPEDVQLAILRSIPGMEKVEMMRNGYAIEYDAMVPTQLWPSL 357 Sbjct: 305 364 Query: 358 ETKRLPGLFTAGQINGTSGYEEXXXXXXXXXXXXXRKVQEKEPVVLDRSQGYIGVLIDDL 417 Sbjct: 365 424 Query: 418 VTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTPIGYEIGLIPKQRYEAFLDKKXXXXXXX 477 Sbjct: 425 --- 481 Query: 478 XXXXXTKVKPVEVNAALAEYESAPI----VDGSNLLTLMRRPEVAYS----FVDLVSPSP 529 Sbjct: 482 ----- 536 Query: 530 EGLDEEMKEQVEIQIKYAGYIEKQLLHVEKLQKMEKKKIPDDINYNEIHGLAMEARQKLT 589 Sbjct: 537 596 Query: 590 KIAPISIGQASRIAGVTPADISILLVHL 617 Sbjct: 597 624 >DR0027 [D] COG0445 NAD/FAD-utilizing enzyme apparently involved in cell division Length = 176 Score = 434 bits (1117), Expect = e-121, Method: Compositional matrix adjust. Identities = 0/589 (0%), Positives = 0/589 (0%), Gaps = 21/589 (3%) Query: 31 CRTLMITINLDMVAFMPCNPSIGGPAKGHVVREIDALGGEMGRNIDKTFIQLRMLNTGKG 90 + I+ + A I+ G +D L G Sbjct: 26 ADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSV 85 Query: 91 PAVHALRAQADKFLYQHAMKETMENTPNLTLRQGMVEELIVENGRCAGVITKTGTVYHSK 150 + K L+ ++++ I Y Sbjct: 86 IRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDL 145 Query: 151 TVILTTGTYLRGKVIMGELTYESGPNNQQPSVRLSENLRELGFDLVRFKTGTPPRVHKDT 210 Sbjct: 146 205 Query: 211 IDFSKTEIQPGDEKPKFFSFETKSSDNEQLPCWLTYTSEVTHQIINDNLHRAPMFTGIIE 270 Sbjct: 206 - 264 Query: 271 GTGPRYCPSIEDKVVRFSDKSQHQIFLEPEGKNTAEYYVQGLSTSLPEDVQLAILRSIPG 330 Sbjct: 265 324 Query: 331 MEKVEMMRNGYAIEYDAMVPTQLWPSLETKRLPGLFTAGQINGTSGYEEXXXXXXXXXXX 390 Sbjct: 325 384 Query: 391 XXRKVQEKEPVVLDRSQGYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTP 450 Sbjct: 385 444 Query: 451 IGYEIGLIPKQRYEAFLDKKXXXXXXXXXXXXTKVKPVEVNAALAEYESAPIVDGSNLLT 510 Sbjct: 445 ------------------ 486 Query: 511 LMRRPEVAYSFVDLVSPS-PEGLDEEMKEQVEIQIKYAGYIEKQLLHVEKLQKMEKKKIP 569 Sbjct: 487 - 545 Query: 570 DDINYNEIHGLAMEARQKLTKIAPISIGQASRIAGVTPADISILLVHLE 618 Sbjct: 546 594 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,527,507 Number of extensions: 1864305 Number of successful extensions: 3365 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 3358 Number of HSP's successfully gapped: 4 Length of query: 628 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 515 Effective length of database: 25,345,689 Effective search space: 13053029835 Effective search space used: 13053029835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)