BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0012.p (338 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ykuT [M] COG0668 Small-conductance mechanosensitive channel 116 6e-26 BH2666 [M] COG0668 Small-conductance mechanosensitive channel 100 3e-21 all1413 [M] COG0668 Small-conductance mechanosensitive channel 96 1e-19 ZybiO_2 [M] COG0668 Small-conductance mechanosensitive channel 87 3e-17 ybiO_2 [M] COG0668 Small-conductance mechanosensitive channel 87 3e-17 >BS_ykuT [M] COG0668 Small-conductance mechanosensitive channel Length = 267 Score = 116 bits (290), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 67/198 (33%), Positives = 100/198 (50%) Query: 77 RFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXXXXXXXXXXXXXQSLVKDVIT 136 R T+ L N+ + F+ ++VL FH+D Q LV D++T Sbjct: 58 RAHTLRSLTLNIFAYTLIFIFFVMVLDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVT 117 Query: 137 GFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTGEVHIIPNGTIINVTNYSLAN 196 GFFI+ E Q VGD I T+ G VE +GLRTT++ S +H IPN I NV+N+S Sbjct: 118 GFFILLEKQLDVGDYITVSTFDGIVEQVGLRTTQIRSFDETLHYIPNRNITNVSNHSRGT 177 Query: 197 ALAVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYPNILGIQSMSTSEYVIRVAAN 256 A+VD+ V ER ++ + ++ + + PN++GIQ + TSE VIRV A Sbjct: 178 MQALVDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIAK 237 Query: 257 CLPNARDAAERQIQSDIK 274 + ER ++ +IK Sbjct: 238 TENMEQWRVERVLRKEIK 255 >BH2666 [M] COG0668 Small-conductance mechanosensitive channel Length = 276 Score = 100 bits (250), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 60/218 (27%), Positives = 108/218 (49%), Gaps = 1/218 (0%) Query: 57 KVIDRSMEKESRGRLLGDSRRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXXXXX 116 ++I S + ++ L S R T+ L N ++ F+ +L+ F + Sbjct: 40 RIISNSFARMAKNNQL-SSGRVVTLEKLSLNAFSYTLMFIFATTLLTIFGLNPSALIAGA 98 Query: 117 XXXXXXXXXXXQSLVKDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLSSTG 176 Q LV D++TGFFI+ E Q VGD + G G VE +GLRT + G Sbjct: 99 GIVGLAIGFGAQGLVSDIVTGFFILLEKQIDVGDYVTAGGVDGIVEEVGLRTALIRGFDG 158 Query: 177 EVHIIPNGTIINVTNYSLANALAVVDVPVKIERGLEATLALIGEALKGIEERNSNVIAYP 236 +H IPN I NV+N+S N A+VD+ + ++ ++++ + + E++ +I P Sbjct: 159 TLHYIPNRNIANVSNHSRGNMRALVDISISYNDNIDEAISVMQKVCDQLAEQDERIIEGP 218 Query: 237 NILGIQSMSTSEYVIRVAANCLPNARDAAERQIQSDIK 274 +++G+Q++ S+ VIR+ A + + ER ++ +K Sbjct: 219 DVIGVQNLGDSDVVIRIIAKTENMEQWSVERLLRKQLK 256 >all1413 [M] COG0668 Small-conductance mechanosensitive channel Length = 536 Score = 95.5 bits (236), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 71/232 (30%), Positives = 105/232 (45%), Gaps = 10/232 (4%) Query: 53 KVVYKVIDR-SMEKESRGRLLGDSR------RFSTVGGLLKNVVTFICNFVMIMLVLSEF 105 ++ Y +IDR + S G LL R ST G+ K++ T +C V I+L L Sbjct: 271 RLTYALIDRFTTTLISSGALLTPEASERMQLRVSTFSGVTKSIATIVCVGVGILLALVAL 330 Query: 106 HFDXXXXXXXXXXXXXXXXXXXQSLVKDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIG 165 D Q+L+KD I GF II EDQ+A+GDVI G G VE + Sbjct: 331 GIDIVPLLAGASLVGVAVSLASQNLIKDAINGFLIILEDQYALGDVINVGNVGGLVENLN 390 Query: 166 LRTTRLLSSTGEVHIIPNGTIINVTNYSLANALAVVDVPVKIERGLEATLALIGEALKGI 225 LR T++ S G + IPN I V N S + A + +PV + ++ L LI + + Sbjct: 391 LRMTQVRDSEGRLITIPNSEIKIVANLSSRWSRADLTIPVDYQADIDQALKLINDVAVKM 450 Query: 226 EER---NSNVIAYPNILGIQSMSTSEYVIRVAANCLPNARDAAERQIQSDIK 274 ++ +I P +LGI + +IRV P + R+ + IK Sbjct: 451 DQDPLWQRQIIETPQVLGIDNFGDRGLIIRVWIKTQPLKQWDVAREYRRRIK 502 >ZybiO_2 [M] COG0668 Small-conductance mechanosensitive channel Length = 291 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 67/227 (29%), Positives = 108/227 (47%), Gaps = 8/227 (3%) Query: 54 VVYKVIDRSMEKESRGRLLGDSRRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXX 113 V+ +I+ + + GR L S R T+ L +N + I + + IM+VLSE + Sbjct: 33 VLASLIENRLASDIHGRPL-PSARTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLL 91 Query: 114 XXXXXXXXXXXXXXQSLVKDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLS 173 Q+LVKD+ITG FI FE+ GD++ G GTVE + +R+ + Sbjct: 92 AGAGALGLAISFGSQTLVKDIITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQ 151 Query: 174 STGEVHIIPNGTIINVTNY--SLANALAVVDVPVKIERGLEATLAL---IGEALKGIEER 228 TG HIIP +I N+ + + +A DV + E +A AL + E ++ EE Sbjct: 152 DTGAYHIIPWSSITTFANFVRGIGSVVANYDVD-RHEDADKANQALKDAVAELMEN-EEI 209 Query: 229 NSNVIAYPNILGIQSMSTSEYVIRVAANCLPNARDAAERQIQSDIKR 275 +I PN GI +S + + +RV+ LP + + S +K+ Sbjct: 210 RGLIIGEPNFAGIVGLSNTAFTLRVSFTTLPLKQWTVRFALDSQVKK 256 >ybiO_2 [M] COG0668 Small-conductance mechanosensitive channel Length = 291 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 67/227 (29%), Positives = 108/227 (47%), Gaps = 8/227 (3%) Query: 54 VVYKVIDRSMEKESRGRLLGDSRRFSTVGGLLKNVVTFICNFVMIMLVLSEFHFDXXXXX 113 V+ +I+ + + GR L S R T+ L +N + I + + IM+VLSE + Sbjct: 33 VLASLIENRLASDIHGRPL-PSARTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLL 91 Query: 114 XXXXXXXXXXXXXXQSLVKDVITGFFIIFEDQFAVGDVIQTGTYKGTVEMIGLRTTRLLS 173 Q+LVKD+ITG FI FE+ GD++ G GTVE + +R+ + Sbjct: 92 AGAGALGLAISFGSQTLVKDIITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQ 151 Query: 174 STGEVHIIPNGTIINVTNY--SLANALAVVDVPVKIERGLEATLAL---IGEALKGIEER 228 TG HIIP +I N+ + + +A DV + E +A AL + E ++ EE Sbjct: 152 DTGAYHIIPWSSITTFANFVRGIGSVVANYDVD-RHEDADKANQALKDAVAELMEN-EEI 209 Query: 229 NSNVIAYPNILGIQSMSTSEYVIRVAANCLPNARDAAERQIQSDIKR 275 +I PN GI +S + + +RV+ LP + + S +K+ Sbjct: 210 RGLIIGEPNFAGIVGLSNTAFTLRVSFTTLPLKQWTVRFALDSQVKK 256 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,127,283 Number of extensions: 565718 Number of successful extensions: 1124 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 1115 Number of HSP's successfully gapped: 35 Length of query: 338 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 231 Effective length of database: 26,037,693 Effective search space: 6014707083 Effective search space used: 6014707083 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)