BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0002.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr7234 [KG] COG1940 Transcriptional regulator/sugar kinase 104 2e-22 HI0144 [KG] COG1940 Transcriptional regulator/sugar kinase 100 5e-21 alr1982 [KG] COG1940 Transcriptional regulator/sugar kinase 98 1e-20 all5002 [KG] COG1940 Transcriptional regulator/sugar kinase 97 3e-20 BH1425 [KG] COG1940 Transcriptional regulator/sugar kinase 95 1e-19 >mlr7234 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 339 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 82/266 (30%), Positives = 116/266 (43%), Gaps = 38/266 (14%) Query: 3 DALVSIDMGGSAARLAIFDR--KVMKMEDSVKIDLGAGTEPLEAVSQLAGVIGRWEQAHK 60 +A ++ID+GG+ R A+ DR K++ P +A + VIG+ E A Sbjct: 4 EAALAIDLGGTELRAALVDRDGKILAFA----------AVPTQAQAGPDVVIGQIE-ALA 52 Query: 61 GEIHTVVAGLA----------GLHDAAGTITTWPNRPAWNGFPFREAFTSATSKPVILFD 110 +H GLA L AG P W P + PV L + Sbjct: 53 ATVHAEAPGLAIVGVGVGAPGPLDPLAGIAVGPPTLAGWQDVPLADILERRLGLPVRLEN 112 Query: 111 DANLAAMGEYRFGLAHPVSPLLYVVVGTGIGSALVMQGDVYEGARGASGELGHITVDPNG 170 DAN AA+GE+RFG H L++V V TGIG +V G + G RG + E+GH+T+ G Sbjct: 113 DANAAALGEWRFGAGHGARSLVFVTVSTGIGGGVVADGRILHGRRGLAAEIGHMTITNEG 172 Query: 171 EPCPCGGFGCLQLYASGRAIERMAAAKGLPITKASDVFRLAAQGNEAARRIIQDS----- 225 E C CG GC + ASG A+ R A A S + RL+A R +++ + Sbjct: 173 ERCVCGVVGCFEAIASGTALGRRANAA-TSAFDGSTLRRLSANAEVTGRHVVEAARLQDD 231 Query: 226 ---------IRLLAIGIANAVRLLDP 242 R L +G N + L P Sbjct: 232 LALALLEEEARWLGVGFTNLLHLYSP 257 >HI0144 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 300 Score = 99.8 bits (247), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 68/218 (31%), Positives = 106/218 (48%), Gaps = 8/218 (3%) Query: 92 FPFREAFTSATSKPVILFDDANLAAMGEYRFGLAHPVSPLLYVVVGTGIGSALVMQGDVY 151 FP + + T KP+ L +DA A EY+ + VS +++ V TG+G +V+ + Sbjct: 85 FPLKASIAKHTDKPIGLLNDAQAATYAEYQLQNSEQVSNFVFITVSTGVGGGIVLNQILQ 144 Query: 152 EGARGASGELGHITVDPNGEPCPCGGFGCLQLYASGRAIERMAAAKGLPITKASDVFRLA 211 G+RG +G +GH DPNG C CG GC++ ASGRAIE +++ P +VF Sbjct: 145 TGSRGIAGHIGHTLADPNGAICGCGRRGCVEAIASGRAIEAVSSQWEEP-CDPKEVFERF 203 Query: 212 AQGNEAARRIIQDSIRLLAIGIANAVRLLDPXXXXXXXXXXXRFPEDYRSLEKAVQQLLG 271 + +E A +++ S + +A IA+ V LD E Y SL V++ L Sbjct: 204 RKNDEKATALVERSAKAIANLIADLVISLD--IQKIAIGGSVGLAEGYLSL---VEKYLQ 258 Query: 272 TLPQ-KNVQVSLSVLGDDAALWGGLAYGLQQVSIKNKE 308 P ++ + G DA L G AY ++ V + E Sbjct: 259 DFPSIYCCEIETAKFGQDAGLIGA-AYWVKDVLLDKPE 295 >alr1982 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 359 Score = 98.2 bits (243), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 14/238 (5%), Positives = 29/238 (12%), Gaps = 21/238 (8%) Query: 78 GTITTWPNRPAWNGFPFREAFTSATSKPVILFDDANLAAMGEYRFGLAHPVSPLLYVVVG 137 + R + + ++A+L L + L Sbjct: 77 LLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQ 136 Query: 138 TGIGSALVMQGDVYEGARGASGELGHITVDPNGEPCPCGGFGCLQLYASGRAIERMA--- 194 + Q G A Sbjct: 137 AEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGG 196 Query: 195 ----------------AAKGLPITKASDVFRLAAQGNEAARRIIQDSIRLLAIGIANAVR 238 G+ + + ++R G Sbjct: 197 ILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYG 256 Query: 239 LLDPXXXXXXXXXXXRFPEDYRSLEKAVQQLLGTLPQKNVQVSLSVLGDDAALWGGLA 296 D L + + D Sbjct: 257 GTWVSERDTRLGVVAMGYGDGYPRAAPSG--TPVLVNGREVPIVGRVAMDMICVDLGP 312 >all5002 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 311 Score = 97.1 bits (240), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 73/290 (25%), Positives = 132/290 (45%), Gaps = 10/290 (3%) Query: 5 LVSIDMGGSAARLAIFDRKVMKMEDSVKIDLGAGTEPLEAVSQLAGVIGRWEQAHKGEIH 64 ++ ID+GG+A +L F + S+ + P + I + + + E Sbjct: 19 VIGIDLGGTAIKLGRFSKD-GTCSQSLTVATPQPATPEAVFLVMVDAIAQIDPDN--ETI 75 Query: 65 TVVAGLAGLHDAAGTITTWP-NRPAWNGFPFREAFTSATSKPVILFDDANLAAMGEYRFG 123 + G G DA G I N P W P + + T+KP ++ +DAN A + E G Sbjct: 76 AIGVGTPGPADAQGRIAQIAINLPQWENVPLADWLEAKTNKPTVIENDANCAGIAEAWLG 135 Query: 124 LAHPVSPLLYVVVGTGIGSALVMQGDVYEGARGASGELGHITVDPNGEPCPCGGFGCLQL 183 + + +GTG+G A+ + G ++ G RGA+GELG IT+ P+G C G G L+ Sbjct: 136 AGRHCQNFIMLTLGTGVGGAIFLDGKLFVGHRGAAGELGLITLQPDGPMCKSGNPGSLEQ 195 Query: 184 YASGRAIERMAAAKGLPITKASDVFRLAAQGNEAARRIIQDSIRLLAIGIANAVRLLDPX 243 Y S +AI R+ + +++ LA G+ A + Q+ + L IG+ + + +L P Sbjct: 196 YTSIKAIRRLTG------KEPAELGMLAQAGDPEALQFWQEYGQNLGIGLTSLIYVLTPQ 249 Query: 244 XXXXXXXXXXRFPEDYRSLEKAVQQLLGTLPQKNVQVSLSVLGDDAALWG 293 +L+ +++ + + + +Q+ + LG+ A + G Sbjct: 250 AIVLGGGVSASCEFFLPTLKAEIEKRVMSTSRAGLQILPAELGNSAGMVG 299 >BH1425 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 330 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 45/107 (42%), Positives = 62/107 (57%), Gaps = 1/107 (0%) Query: 89 WNGFPFREAFTSATSKPVILFDDANLAAMGEYRFGLAHPVSPLLYVVVGTGIGSALVMQG 148 W FP ++ T PVI+ +DAN+AA+GE G +L + +GTG+G +V G Sbjct: 92 WRDFPLKDKLEEETKLPVIVDNDANIAALGEMWKGAGDGAKNMLLITLGTGVGGGIVANG 151 Query: 149 DVYEGARGASGELGHITVDPN-GEPCPCGGFGCLQLYASGRAIERMA 194 ++ G G +GE+GHITV P G PC CG GCL+ AS I R+A Sbjct: 152 NILHGVNGMAGEIGHITVIPEGGAPCNCGKTGCLETVASATGIARIA 198 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,775,908 Number of extensions: 904718 Number of successful extensions: 2999 Number of sequences better than 1.0e-05: 107 Number of HSP's gapped: 2925 Number of HSP's successfully gapped: 107 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)